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Protein

Probable DNA helicase MCM8

Gene

MCM8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable DNA helicase that plays a role in meiotic double-strand break (DSB) repair, but seems not required for recombination with the homologous chromosome. May be involved with RAD51 in a backup pathway that repairs meiotic DSB without giving meiotic crossover, in parallel to the meiotic homologous recombination which relies on DMC1.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 207C4-typeSequence analysisAdd BLAST28
Nucleotide bindingi403 – 410ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • DNA replication initiation Source: InterPro
  • double-strand break repair via homologous recombination Source: TAIR
  • female meiotic nuclear division Source: TAIR
  • male meiotic nuclear division Source: TAIR
  • pollen development Source: TAIR

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair, Meiosis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-68689 CDC6 association with the ORC:origin complex
R-ATH-68867 Assembly of the pre-replicative complex
R-ATH-68949 Orc1 removal from chromatin
R-ATH-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA helicase MCM8 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 8
Short name:
AtMCM8
Gene namesi
Name:MCM8
Ordered Locus Names:At3g09660
ORF Names:F11F8.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G09660
TAIRilocus:2074934 AT3G09660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced fertility and seed numbers due to defects in gametogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004260011 – 801Probable DNA helicase MCM8Add BLAST801

Expressioni

Gene expression databases

ExpressionAtlasiQ9SF37 baseline and differential
GenevisibleiQ9SF37 AT

Structurei

3D structure databases

ProteinModelPortaliQ9SF37
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini351 – 558MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi534 – 537Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri180 – 207C4-typeSequence analysisAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000224129
InParanoidiQ9SF37
KOiK10737
OrthoDBiEOG093602Z8

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR001208 MCM_dom
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SF37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFYGGGSMA SGRPESTGID SLGIGKILAV YIKDNENLAI DEDKLQLTAE
60 70 80 90 100
LIRVFSASPG RDIVSQVNED GGGSFSLSLD LQQFKKISDI ENFFINLEDN
110 120 130 140 150
PKGVIPCMNA AVHKVLFDQW ETNEFENVMK INVRLHNYPE SSISLKNLRA
160 170 180 190 200
AYIGKLVTVH GTVVKVSTVK PLVTQMAFDC GKCKTGITRE FTDGKFSPPL
210 220 230 240 250
KCDSHGCKSK TFTPIRSSAQ TIDFQKIRVQ ELQKPEDHEE GRVPRTVECE
260 270 280 290 300
LMEDLVDICI PGDVVTVTGI IGVINNYMDI GGGKSKTKNQ GFYYLFIEAV
310 320 330 340 350
SVKNTKRQSA FENSEDSSSS AQTADVGDLY SFSQRDLEFI VKFKEEYGSD
360 370 380 390 400
TFRRILHSVC PSIYGHEIVK AGITLSLFGG VRKHSMDRNK VPVRGDIHVI
410 420 430 440 450
IVGDPGLGKS QLLQAAAAIS PRGIYVCGNA TTRAGLTVAV VKDSMTNDYA
460 470 480 490 500
FEAGAMVLAD GGLCCIDEFD KMTTEHQALL EAMEQQCVSV AKAGLVASLS
510 520 530 540 550
ARTSVIAAAN PVGGHYNRAK TVNENLKMSA ALLSRFDLVF ILLDKPDELL
560 570 580 590 600
DKQVSEHIMS LHSASGETSP ALKKFKPVNA ASQNAGYANM HAEGNSLLSR
610 620 630 640 650
LRLDSEKDDD FSPVPGQLLR KYISYARNFV NPKMSKDAGE IIQKFYLKLR
660 670 680 690 700
DHNTSADSTP ITARQLESLV RLAQARARVD LREEITVQDA MDVVEIMKES
710 720 730 740 750
LYDKLIDEHG VVDFGRSGGM SQQKEAKRFL SALDKQSELQ QKDCFSVSEM
760 770 780 790 800
YSLADRIGLR VPDIDTFLEN LNIAGYLLKK GPKTYQVLSS SYSRSQSSRS

R
Length:801
Mass (Da):88,125
Last modified:April 16, 2014 - v2
Checksum:i811B5AA36E2D58C9
GO

Sequence cautioni

The sequence AAF23296 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KC109786 mRNA Translation: AGC54635.1
AC016661 Genomic DNA Translation: AAF23296.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74794.2
RefSeqiNP_001319512.1, NM_001337836.1
UniGeneiAt.40062
At.53240

Genome annotation databases

EnsemblPlantsiAT3G09660.1; AT3G09660.1; AT3G09660
GeneIDi820123
GrameneiAT3G09660.1; AT3G09660.1; AT3G09660
KEGGiath:AT3G09660

Similar proteinsi

Entry informationi

Entry nameiMCM8_ARATH
AccessioniPrimary (citable) accession number: Q9SF37
Secondary accession number(s): L7UY20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health