Reviewed, UniProtKB/Swiss-Prot Q9SEV0 (BAN_ARATH)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin reductase
      Short name=LAR
    EC=1.3.1.77
Alternative name(s):
    Protein BANYULS
    Anthocyanin spotted testa
      Short name=ast

Gene names

Name:	BAN
Ordered Locus Names:	At1g61720
ORF Names:	T13M11.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Produces catechins, precursors of tannins, from leucoanthocyanidin precursors.
Catalytic activity	A flavan-3-ol + 2 NAD(P)+ = an anthocyanidin + 2 NAD(P)H.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subunit structure	Homo- or heterodimer Potential.
Tissue specificity	Flowers and young siliques. Detected specifically in the endothelium of seed coat.
Developmental stage	From fertilization until pre-globular embryo stage.
Miscellaneous	Regulated by TRANSPARENT TESTA 2, TRANSPARENT TESTA 8 and TRANSPARENT TESTA GLABRA 1.
Sequence similarities	Belongs to the dihydroflavonol-4-reductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Coding sequence diversity	Polymorphism
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of flavonoid biosynthetic process Inferred from mutant phenotype. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthocyanidin reductase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Leucoanthocyanidin reductase		PRO_0000215574
Natural variations
	Natural variant	340	1	K → KES in strain: cv. Wassilewskija.
Experimental info
	Sequence conflict	55	1	K → Q in AAF23859. Ref.1
	Sequence conflict	146	1	I → L in AAF23859. Ref.1
	Sequence conflict	156 – 157	2	VE → ID in AAF23859. Ref.1
	Sequence conflict	179	1	T → K in AAF23859. Ref.1
	Sequence conflict	185	1	K → E in AAF23859. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SEV0-1 [UniParc]. Last modified December 13, 2002. Version 2. Checksum: C5C15F7359DB68FB	FASTA	340	37,906
	10 20 30 40 50 60 MDQTLTHTGS KKACVIGGTG NLASILIKHL LQSGYKVNTT VRDPENEKKI AHLRKLQELG 70 80 90 100 110 120 DLKIFKADLT DEDSFESSFS GCEYIFHVAT PINFKSEDPE KDMIKPAIQG VINVLKSCLK 130 140 150 160 170 180 SKSVKRVIYT SSAAAVSINN LSGTGIVMNE ENWTDVEFLT EEKPFNWGYP ISKVLAEKTA 190 200 210 220 230 240 WEFAKENKIN LVTVIPALIA GNSLLSDPPS SLSLSMSFIT GKEMHVTGLK EMQKLSGSIS 250 260 270 280 290 300 FVHVDDLARA HLFLAEKETA SGRYICCAYN TSVPEIADFL IQRYPKYNVL SEFEEGLSIP 310 320 330 340 KLTLSSQKLI NEGFRFEYGI NEMYDQMIEY FESKGLIKAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development." Devic M., Guilleminot J., Debeaujon I., Bechtold N., Bensaude E., Koornneef M., Pelletier G., Delseny M. Plant J. 19:387-398(1999) [PubMed: 10504561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. En-1 and cv. Wassilewskija. Tissue: Silique.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 98-340. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF092912 mRNA. Translation: AAF23859.1. AC005882 Genomic DNA. Translation: AAD21417.1. AK175960 mRNA. Translation: BAD43723.1.
IPI	IPI00523362.
PIR	H96642.
RefSeq	NP_176365.1.
UniGene	At.11057
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9SEV0.
Genome annotation databases
GeneID	842469.
GenomeReviews	Gene locus AT1G61720 in contig CT485782_GR.
KEGG	ath:AT1G61720.
NMPDR	fig|3702.1.peg.5565.
Organism-specific databases
TAIR	At1g61720.
Phylogenomic databases
OMA	Q9SEV0. SINTTIA.
Enzyme and pathway databases
BRENDA	1.3.1.77. 302.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAN_ARATH

Accession

Primary (citable) accession number: Q9SEV0
Secondary accession number(s): Q680A7, Q9SYA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	December 13, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)