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Protein

Chalcone synthase

Gene

CHS

Organism
Cardamine amara (Large bitter-cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
Gene namesi
Name:CHS
OrganismiCardamine amara (Large bitter-cress)
Taxonomic identifieri50461 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCardamineaeCardamine

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 395394Chalcone synthasePRO_0000215963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylvalineBy similarity

Keywords - PTMi

Acetylation

Structurei

3D structure databases

ProteinModelPortaliQ9SEP2.
SMRiQ9SEP2. Positions 8-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SEP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMGDTPSLD EIRKAQRADG PAGILAIGTA NPANYVIQAE YPDYYFRITN
60 70 80 90 100
SEHMTDLKEK FKRMCDKSTI RKRHMHVTEE FLKENPNMCA YMAPSLDARQ
110 120 130 140 150
DIVVVEVPKL GKEAAVKAIK EWGQPKSKIT HLVFCTTSGV DMPGADYQLT
160 170 180 190 200
KLLGLRPSVK RLMMYQQGCF AGGTVLRLAK DLAENNRGAR VLVVCSEITA
210 220 230 240 250
VTFRGPSDTH LDSLVGQALF SDGAAALIVG SDPDTSVGEK PIFEMVSAAQ
260 270 280 290 300
TILPDSDGAI DGHLREVGLT FHLLKDVPGL ISKNIEKSLD EAFKPLGISD
310 320 330 340 350
WNSLFWIAHP GGPAILDQVE IKLGLKEEKM RATRHVMREY GNMSSACVLF
360 370 380 390
ILDEMRKKSA KDGVATTGEG LEWGVLFGFG PGLTVETVVL HSVPL
Length:395
Mass (Da):43,204
Last modified:May 1, 2000 - v1
Checksum:i1DADE8DF44DA7CA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112085 Genomic DNA. Translation: AAF23560.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112085 Genomic DNA. Translation: AAF23560.1.

3D structure databases

ProteinModelPortaliQ9SEP2.
SMRiQ9SEP2. Positions 8-395.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae)."
    Koch M.A., Haubold B., Mitchell-Olds T.
    Mol. Biol. Evol. 17:1483-1498(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Cama.

Entry informationi

Entry nameiCHSY_CARAN
AccessioniPrimary (citable) accession number: Q9SEP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: May 1, 2000
Last modified: March 4, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.