Reviewed, UniProtKB/Swiss-Prot Q9SEK3 (HXK1_SPIOL)

Names and origin

Protein names

Recommended name:
    Hexokinase-1
    EC=2.7.1.1
Alternative name(s):
    SoHxK1

Gene names

Name:

HXK1

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol. Seems not to be involved in cell sugar sensing By similarity.
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast outer membrane; Single-pass membrane protein Ref.1.
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Hexokinase-1		PRO_0000197617
Regions
	Transmembrane	4 – 24	21	Potential
	Nucleotide binding	101 – 106	6	ATP Potential
	Region	171 – 197	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9SEK3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B4C91FF7DB26FE29	FASTA	498	54,135
	10 20 30 40 50 60 MRKAAVGAAV VCTAAVCAAA AVLVRQRMKS SSKWGRVMAI LKELDDNCGT PLGKLRQVAD 70 80 90 100 110 120 AMTVEMHAGL ASEGASKLKM LISYVDNLPT GDEHGLFYAL DLGGTNFRVL RVKLGGKEKR 130 140 150 160 170 180 VVEQEFDEVS IPPELMVGTS EQLFDYIAEA LAKFVATESE GLHPEPNKQR ELGFTFSFPV 190 200 210 220 230 240 KQTSIASGTL IRWTKGFNIE DTVGEDVVAE LTKAMLRKGV DMRVTALVND TVGTLAGGRY 250 260 270 280 290 300 YKEDVIAAVI LGTGTNAAYV ERASAIHKWH GPLPKSGEMV INMEWGNFRS SYLPLTEYDI 310 320 330 340 350 360 ALDEESLNPG EQIFEKMISG MYLGEIVRRV LYRMADEASL FGDTVPSKLK TPFILRTPDM 370 380 390 400 410 420 SAMHHDTSPD LKVVASKLKD VLGIPNSSLK VRKIIVDVCD VIASRGACIS AAGILGIIKK 430 440 450 460 470 480 LGRDTLKQGE NQKSVIALDG GLFEHYAKFR ECMEDSLKEL LGDEVAETIV IEHSNDGSGI 490 GAALLAASHS QYLEEDES

References

[1]	"Spinach hexokinase I is located in the outer envelope membrane of plastids." Wiese A., Groener F., Sonnewald U., Deppner H., Lerchl J., Fluegge U.I., Weber A. FEBS Lett. 461:13-18(1999) [PubMed: 10561488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF118132 mRNA. Translation: AAF18584.1.
3D structure databases
SMR	Q9SEK3. Positions 46-491.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.1. 286.
Family and domain databases
InterPro	IPR001312. Hexokinase. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProtoNet	Search...

Entry information

Entry name

HXK1_SPIOL

Accession

Primary (citable) accession number: Q9SEK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)