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Protein

26S proteasome regulatory subunit 6B homolog

Gene

RPT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi196 – 203ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.8. 399.
ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5687128. MAPK6/MAPK4 signaling.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-ATH-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 6B homolog
Alternative name(s):
26S protease subunit 6B homolog
26S proteasome AAA-ATPase subunit RPT3
Protein BMAA insensitive morphology 409
Regulatory particle triple-A ATPase subunit 3
Gene namesi
Name:RPT3
Synonyms:BIM409
Ordered Locus Names:At5g58290
ORF Names:MCK7.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G58290.
TAIRilocus:2161258. AT5G58290.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • cytosolic proteasome complex Source: GO_Central
  • membrane Source: TAIR
  • nuclear proteasome complex Source: GO_Central
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR
  • proteasome complex Source: TAIR

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158G → E: Impairs the light-specific hypocotyl elongation response elicited by a glutamate receptor agonist, BMAA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000846922 – 40826S proteasome regulatory subunit 6B homologAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9SEI4.
PRIDEiQ9SEI4.

PTM databases

iPTMnetiQ9SEI4.

Expressioni

Tissue specificityi

Expressed in dark-grown etiolated seedlings, roots, leaves, stems and flowers.

Gene expression databases

ExpressionAtlasiQ9SEI4. baseline and differential.
GenevisibleiQ9SEI4. AT.

Interactioni

Subunit structurei

Component of the 19S regulatory particule (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi21185. 8 interactors.
IntActiQ9SEI4. 7 interactors.
STRINGi3702.AT5G58290.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SEI4.
SMRiQ9SEI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 75Sequence analysisAdd BLAST48

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0727. Eukaryota.
COG1222. LUCA.
HOGENOMiHOG000225143.
KOiK03063.
OMAiLIIRNDP.
OrthoDBiEOG09360AE8.
PhylomeDBiQ9SEI4.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR035256. PSMC4.
PANTHERiPTHR23073:SF50. PTHR23073:SF50. 1 hit.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAAVASMV LDPKASPALM DLSTADEEDL YGRLKSLERQ LEFTDIQEEY
60 70 80 90 100
VKDEQKNLKR ELLRAQEEVK RIQSVPLVIG QFMEMVDQNN GIVGSTTGSN
110 120 130 140 150
YYVRILSTIN RELLKPSASV ALHRHSNALV DVLPPEADSS ISLLSQSEKP
160 170 180 190 200
DVSYNDIGGC DIQKQEIREA VELPLTHHEL YKQIGIDPPR GVLLYGPPGT
210 220 230 240 250
GKTMLAKAVA NHTTAAFIRV VGSEFVQKYL GEGPRMVRDV FRLAKENAPA
260 270 280 290 300
IIFIDEVDAI ATARFDAQTG ADREVQRILM ELLNQMDGFD QTVNVKVIMA
310 320 330 340 350
TNRADTLDPA LLRPGRLDRK IEFPLPDRRQ KRLVFQVCTS KMNLSDEVDL
360 370 380 390 400
EDYVSRPDKI SAAEIAAICQ EAGMHAVRKN RYVILPKDFE KGYRANVKKP

DTDFEFYK
Length:408
Mass (Da):45,751
Last modified:May 1, 2000 - v1
Checksum:iA1045D85BF3BBEE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123392 mRNA. Translation: AAF22523.1.
AB019228 Genomic DNA. Translation: BAA96920.1.
CP002688 Genomic DNA. Translation: AED97029.1.
AY070466 mRNA. Translation: AAL49932.1.
BT020373 mRNA. Translation: AAV85728.1.
RefSeqiNP_200637.1. NM_125214.6.
UniGeneiAt.45955.

Genome annotation databases

EnsemblPlantsiAT5G58290.1; AT5G58290.1; AT5G58290.
GeneIDi835941.
GrameneiAT5G58290.1; AT5G58290.1; AT5G58290.
KEGGiath:AT5G58290.

Similar proteinsi

Entry informationi

Entry nameiPRS6B_ARATH
AccessioniPrimary (citable) accession number: Q9SEI4
Secondary accession number(s): Q5PNS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families