Q9SEH7 (PMT2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putrescine N-methyltransferase 2
Short name=PMT 2
EC=2.1.1.53

Gene names

Name:

PMT2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + putrescine = S-adenosyl-L-homocysteine + N-methylputrescine.
Pathway	Alkaloid biosynthesis; nicotine biosynthesis.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	putrescine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Putrescine N-methyltransferase 2		PRO_0000156542
Regions
	Region	201 – 202	2	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	220	1	Proton acceptor By similarity
	Binding site	95	1	S-adenosyl-L-methionine By similarity
	Binding site	170	1	S-adenosyl-L-methionine By similarity
	Binding site	289	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SEH7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DD2E024121D5111D	FASTA	353	38,746
	10 20 30 40 50 60 MEVISTNTNG STIFKSGAIP MNGHHNGTSK HQNGHKNGTS EQQNGTISLD NGNELLGNSN 70 80 90 100 110 120 CIKPGWFSEF SALWPGEAFS LKVEKLLFQG KSDYQDVMLF ESATYGKVLT LDGAIQHTEN 130 140 150 160 170 180 GGFPYTEMIV HLPLGSIPNP KKVLIIGGGI GFTLFEMLRY PTIEKIDIVE IDDVVVDVSR 190 200 210 220 230 240 KFFPYLAANF NDPRVTLVLG DGAAFVKAAQ AEYYDAIIVD SSDPIGPAKD LFERPFFEAV 250 260 270 280 290 300 AKALRPGGVV CTQAESIWLH MHIIKQIIAN CRQVFKGSVN YAWTTVPTYP TGVIGYMLCS 310 320 330 340 350 TEGPEIDFKN PVNPIDKETA QVKSKLAPLK FYNSDIHKAA FILPSFARSM IES

References

[1]	"Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco." Riechers D.E., Timko M.P. Plant Mol. Biol. 41:387-401(1999) [PubMed: 10598105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Xanthi.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF126809 Genomic DNA. Translation: AAF14878.1.
3D structure databases
ProteinModelPortal	Q9SEH7.
SMR	Q9SEH7. Positions 64-352.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. SpeE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT2_TOBAC

Accession

Primary (citable) accession number: Q9SEH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	September 21, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program