Reviewed, UniProtKB/Swiss-Prot Q9SEH5 (PMT3_TOBAC)

Names and origin

Protein names

Recommended name:
    Putrescine N-methyltransferase 3
      Short name=PMT 3
    EC=2.1.1.53

Gene names

Name:

PMT3

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + putrescine = S-adenosyl-L-homocysteine + N-methylputrescine.
Pathway	Alkaloid biosynthesis; nicotine biosynthesis.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	putrescine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Putrescine N-methyltransferase 3		PRO_0000156543
Regions
	Region	229 – 230	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	123	1	S-adenosyl-L-methionine By similarity
	Binding site	198	1	S-adenosyl-L-methionine By similarity
	Binding site	248	1	S-adenosyl-L-methionine By similarity
	Binding site	317	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SEH5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A19DE2B83CE4D530	FASTA	381	41,795
	10 20 30 40 50 60 MEVISTNTNG STIFKNGAIP MNGYQNGTSK HQNGHQNGTS EHRNGHQNGI SEHQNGHQNG 70 80 90 100 110 120 TSEHQNGHQN GTISHDNGNE LQLLGSSNSI KPGWFSEFSA LWPGEAFSLK VEKLLFQGKS 130 140 150 160 170 180 DYQDVMLFES ATYGKVLTLD GAIQHTENGG FPYTEMIVHL PLGSIPNPKK VLIIGGGIGF 190 200 210 220 230 240 TLFEMLRYPT IEKIDIVEID DVVVDVSRKF FPYLAANFSD PRVTLVLGDG AAFVKAAQAG 250 260 270 280 290 300 YYDAIIVDSS DPIGPAKDLF ERPFFEAVAK ALRPGGVVCT QAESIWLHMH IIKQIIANCR 310 320 330 340 350 360 QVFKGSVNYA WTTVPTYPTG VIGYMLCSTE GPEVDFKNPV NPIDKETTQV KSKLAPLKFY 370 380 NSDIHKAAFI LPSFARSMIE S

References

[1]

"Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco." Riechers D.E., Timko M.P. Plant Mol. Biol. 41:387-401(1999) [PubMed: 10598105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Xanthi.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF126811 Genomic DNA. Translation: AAF14880.1.
3D structure databases
SMR	Q9SEH5. Positions 88-380.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.53. 298.
Family and domain databases
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT3_TOBAC

Accession

Primary (citable) accession number: Q9SEH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)