Reviewed, UniProtKB/Swiss-Prot Q9SEH4 (PMT4_TOBAC)

Names and origin

Protein names

Recommended name:
    Putrescine N-methyltransferase 4
      Short name=PMT 4
    EC=2.1.1.53

Gene names

Name:

PMT4

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + putrescine = S-adenosyl-L-homocysteine + N-methylputrescine.
Pathway	Alkaloid biosynthesis; nicotine biosynthesis.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	putrescine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Putrescine N-methyltransferase 4		PRO_0000156544
Regions
	Region	267 – 268	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	161	1	S-adenosyl-L-methionine By similarity
	Binding site	236	1	S-adenosyl-L-methionine By similarity
	Binding site	286	1	S-adenosyl-L-methionine By similarity
	Binding site	355	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SEH4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BDEE4417C454ED16	FASTA	419	45,899
	10 20 30 40 50 60 MEVISTNTNG STIFKNGAIP MNGHQSGTSK HLNGYQNGTS KHQNGHHNGT SEHRNGHQNG 70 80 90 100 110 120 ISEHQNGHQN GTSEHRNGHQ NGISEHQNGH QNGTSEHQNG HQNGTSEQQN GTISHDNGNE 130 140 150 160 170 180 LLGNSNSIKL GWFSEFSALW PGEAFSLKVE KLLFQGKSDY QDVMLFESAT YGKVLTLDGA 190 200 210 220 230 240 IQHTENGGFP YTEMIVHLPL GSIPNPKKVL IIGGGIGFTL FEMLRYPTIE KIDIVEIDDV 250 260 270 280 290 300 VVDVSRKSFP YLAANFNDPR VTLVLGDGAA FVKAAQAGYY DAIIVDSSDP IGPAKDLFER 310 320 330 340 350 360 PFFEAVAKAL RPGGVVCTQA ESIWLHMHII KQIIANCRQV FKGSVNYAWT TVPTYPTGVI 370 380 390 400 410 GYMLCSTEGP EVDFKNPINP IDKETTQVKS KLAPLKFYNS DIHKAAFILP SFARSMIES

References

[1]

"Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco." Riechers D.E., Timko M.P. Plant Mol. Biol. 41:387-401(1999) [PubMed: 10598105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Xanthi.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF126812 Genomic DNA. Translation: AAF14881.1.
3D structure databases
SMR	Q9SEH4. Positions 131-418.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.53. 298.
Family and domain databases
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT4_TOBAC

Accession

Primary (citable) accession number: Q9SEH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)