UniProtKB - Q9SEE5 (GALK1_ARATH)
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Protein
Galactokinase
Gene
GAL1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Sugar-1-kinase with a very high substrate specificity for the alpha-anomeric configuration of D-galacose (D-Gal). Converts also efficiently 2-deoxy-D-Gal to 2-deoxy-D-al-1-phosphate.2 Publications
Catalytic activityi
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.1 Publication
Cofactori
Mg2+1 Publication, Mn2+1 Publication, Ca2+1 PublicationNote: Magnesium. Can also use other divalent cations like manganese or calcium.1 Publication
Kineticsi
- KM=701 µM for D-galactose1 Publication
- KM=701 µM for ATP1 Publication
- Vmax=3.5 µmol/min/µg enzyme toward D-galactose1 Publication
- Vmax=3.3 µmol/min/µg enzyme toward ATP1 Publication
pH dependencei
Optimum pH is 7-8.1 Publication
Temperature dependencei
Optimum temperature is 30-42 degrees Celsius.1 Publication
: galactose metabolism Pathwayi
This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 56 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 210 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 159 – 169 | ATPSequence analysisAdd BLAST | 11 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- galactokinase activity Source: TAIR
- galactose binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- carbohydrate phosphorylation Source: TAIR
- galactose metabolic process Source: TAIR
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism, Galactose metabolism |
Ligand | ATP-binding, Calcium, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G64440-MONOMER. ARA:AT3G06580-MONOMER. MetaCyc:AT3G06580-MONOMER. |
UniPathwayi | UPA00214. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GAL1 Synonyms:GALK Ordered Locus Names:At3g06580 ORF Names:F5E6.9 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G06580. |
TAIRi | locus:2084344. AT3G06580. |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 62 | E → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 206 | S → G: Decreased specificity and phosphorylation of GalNAc. 1 Publication | 1 | |
Mutagenesisi | 262 | Y → F: No effect on specificity or activity. 1 Publication | 1 | |
Mutagenesisi | 437 | A → S: Reduced phosphorylation activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000184651 | 2 – 496 | GalactokinaseAdd BLAST | 495 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q9SEE5. |
PRIDEi | Q9SEE5. |
PTM databases
iPTMneti | Q9SEE5. |
Expressioni
Tissue specificityi
Expressed in roots, stems, leaves, flowers and young siliques. Higher expression in the elongating middle stem region than in the lower or upper stem region.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SEE5. baseline and differential. |
Genevisiblei | Q9SEE5. AT. |
Interactioni
Protein-protein interaction databases
BioGridi | 5172. 2 interactors. |
STRINGi | 3702.AT3G06580.1. |
Structurei
3D structure databases
ProteinModelPortali | Q9SEE5. |
SMRi | Q9SEE5. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0631. Eukaryota. COG0153. LUCA. |
HOGENOMi | HOG000241099. |
InParanoidi | Q9SEE5. |
KOi | K18674. |
OMAi | YECSHPD. |
OrthoDBi | EOG0936072Y. |
PhylomeDBi | Q9SEE5. |
Family and domain databases
Gene3Di | 3.30.230.10. 2 hits. 3.30.70.890. 1 hit. |
InterProi | View protein in InterPro IPR000705. Galactokinase. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal-bd. IPR013750. GHMP_kinase_C_dom. IPR036554. GHMP_kinase_C_sf. IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. |
Pfami | View protein in Pfam PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. |
PIRSFi | PIRSF000530. Galactokinase. 1 hit. |
PRINTSi | PR00473. GALCTOKINASE. |
SUPFAMi | SSF54211. SSF54211. 1 hit. SSF55060. SSF55060. 2 hits. |
TIGRFAMsi | TIGR00131. gal_kin. 1 hit. |
PROSITEi | View protein in PROSITE PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SEE5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKPEEVSVP IFTSLEPVYG EGSLLQEATQ RFDVLKANFN DVFGASPQLF
60 70 80 90 100
ARSPGRVNLI GEHIDYEGYS VLPMAIRQDT IIAIRKCEDQ KQLRIANVND
110 120 130 140 150
KYTMCTYPAD PDQEIDLKNH KWGHYFICAY KGFHEYAKSK GVNLGSPVGL
160 170 180 190 200
DVLVDGIVPT GSGLSSSAAF VCSATIAIMA VFGHNFEKKE LAQLTCECER
210 220 230 240 250
HIGTQSGGMD QAISIMAKTG FAELIDFNPV RATDVKLPDG GSFVIAHSLA
260 270 280 290 300
ESQKAVTAAK NYNNRVVECR LASIILGVKL GMEPKEAISK VKTLSDVEGL
310 320 330 340 350
CVSFAGDRGS SDPLLAVKEY LKEEPYTAEE IEKILEEKLP SIVNNDPTSL
360 370 380 390 400
AVLNAATHFK LHQRAAHVYS EARRVHGFKD TVNSNLSDEE KLKKLGDLMN
410 420 430 440 450
ESHYSCSVLY ECSCPELEEL VQVCKENGAL GARLTGAGWG GCAVALVKEF
460 470 480 490
DVTQFIPAVK EKYYKKRVEK GVVKKEDMEL YLFASKPSSG AAIFNL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 274 – 304 | IILGV…LCVSF → DQYLVLSSEWNQKKQYQKLR LFLMWRDYVCHS in CAA68163 (PubMed:9225860).CuratedAdd BLAST | 31 | |
Sequence conflicti | 351 | A → T in AAB94084 (PubMed:10344205).Curated | 1 | |
Sequence conflicti | 402 | S → T in CAA68163 (PubMed:9225860).Curated | 1 | |
Sequence conflicti | 429 | A → P in AAF15552 (Ref. 3) Curated | 1 | |
Sequence conflicti | 464 – 465 | YK → HN in CAA68163 (PubMed:9225860).Curated | 2 | |
Sequence conflicti | 484 | A → G in CAA68163 (PubMed:9225860).Curated | 1 | |
Sequence conflicti | 491 | A → S in CAA68163 (PubMed:9225860).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X99851 mRNA. Translation: CAA68163.1. AF024623 mRNA. Translation: AAB94084.1. AF152851 Genomic DNA. Translation: AAF15552.1. AC020580 Genomic DNA. Translation: AAG51339.1. CP002686 Genomic DNA. Translation: AEE74417.1. AY136356 mRNA. Translation: AAM97022.1. BT010374 mRNA. Translation: AAQ56817.1. |
PIRi | T51592. |
RefSeqi | NP_187310.1. NM_111534.4. |
UniGenei | At.21429. |
Genome annotation databases
EnsemblPlantsi | AT3G06580.1; AT3G06580.1; AT3G06580. |
GeneIDi | 819837. |
Gramenei | AT3G06580.1; AT3G06580.1; AT3G06580. |
KEGGi | ath:AT3G06580. |
Similar proteinsi
Entry informationi
Entry namei | GALK1_ARATH | |
Accessioni | Q9SEE5Primary (citable) accession number: Q9SEE5 Secondary accession number(s): O24021, O48936 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 28, 2018 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |