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Q9SE94

- MTHR1_MAIZE

UniProt

Q9SE94 - MTHR1_MAIZE

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Protein
Methylenetetrahydrofolate reductase 1
Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptor By similarity
Binding sitei81 – 811FAD By similarity
Binding sitei113 – 1131Substrate By similarity
Binding sitei153 – 1531FAD By similarity
Binding sitei175 – 1751FAD By similarity
Binding sitei182 – 1821FAD By similarity
Binding sitei193 – 1931Substrate By similarity
Binding sitei285 – 2851Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NAD By similarity
Nucleotide bindingi52 – 532NAD and FAD By similarity
Nucleotide bindingi111 – 1133FAD By similarity
Nucleotide bindingi157 – 1604FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Alternative name(s):
ZmMTHFR1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ9SE94.
MaizeGDBi146840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Methylenetetrahydrofolate reductase 1
PRO_0000190251Add
BLAST

Proteomic databases

PRIDEiQ9SE94.
ProMEXiQ9SE94.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9SE94.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246234.
KOiK00297.
OMAiYLEFFVS.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SE94-1 [UniParc]FASTAAdd to Basket

« Hide

MKVIEKILEA AGDGRTAFSF EYFPPKTEEG VENLFERMDR MVAHGPSFCD    50
ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDHALETIK 100
SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVQHIRA KYGDYFGITV 150
AGYPEAHPDA IQGEGGATLE AYSNDLAYLK RKVDAGADLI VTQLFYDTDI 200
FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPSEITAALD 250
PIKDNEEAVR QYGIHLGTEM CKKILATGIK TLHLYTLNMD KSAIGILMNL 300
GLIEESKVSR PLPWRPATNV FRVKEDVRPI FWANRPKSYL KRTLGWDQYP 350
HGRWGDSRNP SYGALTDHQF TRPRGRGKKL QEEWAVPLKS VEDISERFTN 400
FCQGKLTSSP WSELDGLQPE TKIIDDQLVN INQKGFLTIN SQPAVNGEKS 450
DSPTVGWGGP GGYVYQKAYL EFFCAKEKLD QLIEKIKAFP SLTYIAVNKD 500
GETFSNISPN AVNAVTWGVF PGKEIIQPTV VDHASFMVWK DEAFEIWTRG 550
WGCMFPEGDS SRELLEKVQK TYYLVSLVDN DYVQGDLFAA FKI 593
Length:593
Mass (Da):66,429
Last modified:May 1, 2000 - v1
Checksum:i4568996775B638B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF174486 mRNA. Translation: AAD51733.1.
RefSeqiNP_001104947.1. NM_001111477.1.
UniGeneiZm.475.

Genome annotation databases

GeneIDi541794.
KEGGizma:541794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF174486 mRNA. Translation: AAD51733.1 .
RefSeqi NP_001104947.1. NM_001111477.1.
UniGenei Zm.475.

3D structure databases

ProteinModelPortali Q9SE94.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9SE94.
ProMEXi Q9SE94.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 541794.
KEGGi zma:541794.

Organism-specific databases

Gramenei Q9SE94.
MaizeGDBi 146840.

Phylogenomic databases

HOGENOMi HOG000246234.
KOi K00297.
OMAi YLEFFVS.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
    Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
    J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION.

Entry informationi

Entry nameiMTHR1_MAIZE
AccessioniPrimary (citable) accession number: Q9SE94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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