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Protein

Methylenetetrahydrofolate reductase 1

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptorBy similarity
Binding sitei81 – 811FADBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei175 – 1751FADBy similarity
Binding sitei182 – 1821FADBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei285 – 2851SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NADBy similarity
Nucleotide bindingi52 – 532NAD and FADBy similarity
Nucleotide bindingi111 – 1133FADBy similarity
Nucleotide bindingi157 – 1604FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BRENDAi1.5.1.20. 6752.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Alternative name(s):
ZmMTHFR1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Chromosome 1

Organism-specific databases

GrameneiQ9SE94.
MaizeGDBi146840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Methylenetetrahydrofolate reductase 1PRO_0000190251Add
BLAST

Proteomic databases

PRIDEiQ9SE94.
ProMEXiQ9SE94.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9SE94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246234.
KOiK00297.
OMAiYLEFFVS.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SE94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVIEKILEA AGDGRTAFSF EYFPPKTEEG VENLFERMDR MVAHGPSFCD
60 70 80 90 100
ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDHALETIK
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVQHIRA KYGDYFGITV
160 170 180 190 200
AGYPEAHPDA IQGEGGATLE AYSNDLAYLK RKVDAGADLI VTQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPSEITAALD
260 270 280 290 300
PIKDNEEAVR QYGIHLGTEM CKKILATGIK TLHLYTLNMD KSAIGILMNL
310 320 330 340 350
GLIEESKVSR PLPWRPATNV FRVKEDVRPI FWANRPKSYL KRTLGWDQYP
360 370 380 390 400
HGRWGDSRNP SYGALTDHQF TRPRGRGKKL QEEWAVPLKS VEDISERFTN
410 420 430 440 450
FCQGKLTSSP WSELDGLQPE TKIIDDQLVN INQKGFLTIN SQPAVNGEKS
460 470 480 490 500
DSPTVGWGGP GGYVYQKAYL EFFCAKEKLD QLIEKIKAFP SLTYIAVNKD
510 520 530 540 550
GETFSNISPN AVNAVTWGVF PGKEIIQPTV VDHASFMVWK DEAFEIWTRG
560 570 580 590
WGCMFPEGDS SRELLEKVQK TYYLVSLVDN DYVQGDLFAA FKI
Length:593
Mass (Da):66,429
Last modified:May 1, 2000 - v1
Checksum:i4568996775B638B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174486 mRNA. Translation: AAD51733.1.
RefSeqiNP_001104947.1. NM_001111477.1.
UniGeneiZm.475.

Genome annotation databases

EnsemblPlantsiGRMZM2G347056_T01; GRMZM2G347056_P01; GRMZM2G347056.
GeneIDi541794.
KEGGizma:541794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174486 mRNA. Translation: AAD51733.1.
RefSeqiNP_001104947.1. NM_001111477.1.
UniGeneiZm.475.

3D structure databases

ProteinModelPortaliQ9SE94.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9SE94.
ProMEXiQ9SE94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM2G347056_T01; GRMZM2G347056_P01; GRMZM2G347056.
GeneIDi541794.
KEGGizma:541794.

Organism-specific databases

GrameneiQ9SE94.
MaizeGDBi146840.

Phylogenomic databases

HOGENOMiHOG000246234.
KOiK00297.
OMAiYLEFFVS.

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi1.5.1.20. 6752.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
    Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
    J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION.

Entry informationi

Entry nameiMTHR1_MAIZE
AccessioniPrimary (citable) accession number: Q9SE94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.