Methylenetetrahydrofolate reductase 1

Functionⁱ

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityⁱ

5-methyltetrahydrofolate + NAD(P)⁺ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactorⁱ

FADBy similarity

Enzyme regulationⁱ

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayⁱ: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	21	Proton donor/acceptorBy similarity	1
Binding siteⁱ	81	FADBy similarity	1
Binding siteⁱ	113	SubstrateBy similarity	1
Binding siteⁱ	153	FADBy similarity	1
Binding siteⁱ	175	FADBy similarity	1
Binding siteⁱ	182	FADBy similarity	1
Binding siteⁱ	193	SubstrateBy similarity	1
Binding siteⁱ	285	SubstrateBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	21 – 26	NADBy similarity	6
Nucleotide bindingⁱ	52 – 53	NAD and FADBy similarity	2
Nucleotide bindingⁱ	111 – 113	FADBy similarity	3
Nucleotide bindingⁱ	157 – 160	FADBy similarity	4

GO - Molecular functionⁱ

methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

methionine metabolic process Source: InterPro
tetrahydrofolate interconversion Source: UniProtKB-UniPathway

Complete GO annotation...

Keywordsⁱ

Molecular function	Oxidoreductase
Ligand	FAD, Flavoprotein, NAD

Enzyme and pathway databases

BRENDAⁱ	1.5.1.20. 6752.
UniPathwayⁱ	UPA00193.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20) Alternative name(s): ZmMTHFR1
Organismⁱ	Zea mays (Maize)
Taxonomic identifierⁱ	4577 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Tripsacinae › Zea
Proteomesⁱ	UP000007305 Componentⁱ: Unplaced

Organism-specific databases

MaizeGDBⁱ	146840.

MaizeGDBⁱ

146840.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000190251	1 – 593	Methylenetetrahydrofolate reductase 1Add BLAST		593

Proteomic databases

PaxDbⁱ	Q9SE94.
PRIDEⁱ	Q9SE94.
Protein Mass spectra EXtraction More...ProMEXⁱ	Q9SE94.

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9SE94. baseline and differential.
Genevisibleⁱ	Q9SE94. ZM.

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

STRINGⁱ	4577.GRMZM2G347056_P01.

STRINGⁱ

4577.GRMZM2G347056_P01.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9SE94.
SMRⁱ	Q9SE94.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the methylenetetrahydrofolate reductase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0564. Eukaryota. COG0685. LUCA.
HOGENOMⁱ	HOG000246234.
KEGG Orthology (KO) More...KOⁱ	K00297.
OMAⁱ	MESGDKW.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093604CD.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00537. MTHFR. 1 hit.
Gene3Dⁱ	3.20.20.220. 1 hit.
InterProⁱ	View protein in InterPro IPR029041. FAD-linked_oxidoreductase-like. IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02219. MTHFR. 1 hit.
SUPFAMⁱ	SSF51730. SSF51730. 1 hit.
TIGRFAMsⁱ	TIGR00677. fadh2_euk. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9SE94-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKVIEKILEA AGDGRTAFSF EYFPPKTEEG VENLFERMDR MVAHGPSFCD 
        60         70         80         90        100
ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDHALETIK 
       110        120        130        140        150
SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVQHIRA KYGDYFGITV 
       160        170        180        190        200
AGYPEAHPDA IQGEGGATLE AYSNDLAYLK RKVDAGADLI VTQLFYDTDI 
       210        220        230        240        250
FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPSEITAALD 
       260        270        280        290        300
PIKDNEEAVR QYGIHLGTEM CKKILATGIK TLHLYTLNMD KSAIGILMNL 
       310        320        330        340        350
GLIEESKVSR PLPWRPATNV FRVKEDVRPI FWANRPKSYL KRTLGWDQYP 
       360        370        380        390        400
HGRWGDSRNP SYGALTDHQF TRPRGRGKKL QEEWAVPLKS VEDISERFTN 
       410        420        430        440        450
FCQGKLTSSP WSELDGLQPE TKIIDDQLVN INQKGFLTIN SQPAVNGEKS 
       460        470        480        490        500
DSPTVGWGGP GGYVYQKAYL EFFCAKEKLD QLIEKIKAFP SLTYIAVNKD 
       510        520        530        540        550
GETFSNISPN AVNAVTWGVF PGKEIIQPTV VDHASFMVWK DEAFEIWTRG 
       560        570        580        590 
WGCMFPEGDS SRELLEKVQK TYYLVSLVDN DYVQGDLFAA FKI

Length:593

Mass (Da):66,429

Last modified:May 1, 2000 - v1

Checksum:ⁱ4568996775B638B4

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF174486 mRNA. Translation: AAD51733.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001104947.1. NM_001111477.2.
UniGeneⁱ	Zm.475.

Genome annotation databases

EnsemblPlantsⁱ	Zm00001d034602_T001; Zm00001d034602_P001; Zm00001d034602.
GeneIDⁱ	541794.
Grameneⁱ	Zm00001d034602_T001; Zm00001d034602_P001; Zm00001d034602.
KEGGⁱ	zma:541794.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF174486 mRNA. Translation: AAD51733.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001104947.1. NM_001111477.2.
UniGeneⁱ	Zm.475.

3D structure databases

ProteinModelPortalⁱ	Q9SE94.
SMRⁱ	Q9SE94.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	4577.GRMZM2G347056_P01.

STRINGⁱ

4577.GRMZM2G347056_P01.

Proteomic databases

PaxDbⁱ	Q9SE94.
PRIDEⁱ	Q9SE94.
Protein Mass spectra EXtraction More...ProMEXⁱ	Q9SE94.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	Zm00001d034602_T001; Zm00001d034602_P001; Zm00001d034602.
GeneIDⁱ	541794.
Grameneⁱ	Zm00001d034602_T001; Zm00001d034602_P001; Zm00001d034602.
KEGGⁱ	zma:541794.

Organism-specific databases

MaizeGDBⁱ	146840.

MaizeGDBⁱ

146840.

Phylogenomic databases

eggNOGⁱ	KOG0564. Eukaryota. COG0685. LUCA.
HOGENOMⁱ	HOG000246234.
KEGG Orthology (KO) More...KOⁱ	K00297.
OMAⁱ	MESGDKW.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093604CD.

Enzyme and pathway databases

UniPathwayⁱ	UPA00193.
BRENDAⁱ	1.5.1.20. 6752.

Gene expression databases

ExpressionAtlasⁱ	Q9SE94. baseline and differential.
Genevisibleⁱ	Q9SE94. ZM.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00537. MTHFR. 1 hit.
Gene3Dⁱ	3.20.20.220. 1 hit.
InterProⁱ	View protein in InterPro IPR029041. FAD-linked_oxidoreductase-like. IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02219. MTHFR. 1 hit.
SUPFAMⁱ	SSF51730. SSF51730. 1 hit.
TIGRFAMsⁱ	TIGR00677. fadh2_euk. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MTHR1_MAIZE
Accessionⁱ	Q9SE94Primary (citable) accession number: Q9SE94
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	May 1, 2000
	Last modified:	July 5, 2017
	This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9SE94 (MTHR1_MAIZE)

Your basket is currently empty.

Methylenetetrahydrofolate reductase 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tetrahydrofolate interconversion

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Enzyme regulationⁱ

Pathwayⁱ: tetrahydrofolate interconversion

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ