Reviewed, UniProtKB/Swiss-Prot Q9SE94 (MTHR1_MAIZE)

Names and origin

Protein names	Recommended name:     Methylenetetrahydrofolate reductase 1     EC=1.5.1.20 Alternative name(s):     ZmMTHFR1
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch. Ref.1
Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine. Ref.1
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Methylenetetrahydrofolate reductase 1		PRO_0000190251

Sequences

	Sequence		Length	Mass (Da)
	Q9SE94-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4568996775B638B4	FASTA	593	66,429
	10 20 30 40 50 60 MKVIEKILEA AGDGRTAFSF EYFPPKTEEG VENLFERMDR MVAHGPSFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDHALETIK SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVEGGFAC ALDLVQHIRA KYGDYFGITV AGYPEAHPDA IQGEGGATLE AYSNDLAYLK 190 200 210 220 230 240 RKVDAGADLI VTQLFYDTDI FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK 250 260 270 280 290 300 IPSEITAALD PIKDNEEAVR QYGIHLGTEM CKKILATGIK TLHLYTLNMD KSAIGILMNL 310 320 330 340 350 360 GLIEESKVSR PLPWRPATNV FRVKEDVRPI FWANRPKSYL KRTLGWDQYP HGRWGDSRNP 370 380 390 400 410 420 SYGALTDHQF TRPRGRGKKL QEEWAVPLKS VEDISERFTN FCQGKLTSSP WSELDGLQPE 430 440 450 460 470 480 TKIIDDQLVN INQKGFLTIN SQPAVNGEKS DSPTVGWGGP GGYVYQKAYL EFFCAKEKLD 490 500 510 520 530 540 QLIEKIKAFP SLTYIAVNKD GETFSNISPN AVNAVTWGVF PGKEIIQPTV VDHASFMVWK 550 560 570 580 590 DEAFEIWTRG WGCMFPEGDS SRELLEKVQK TYYLVSLVDN DYVQGDLFAA FKI

References

[1]

"Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants." Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D. J. Biol. Chem. 274:36089-36096(1999) [PubMed: 10593891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF174486 mRNA. Translation: AAD51733.1.
RefSeq	NP_001104947.1.
UniGene	Zm.475
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Genome annotation databases
GeneID	541794.
Organism-specific databases
Gramene	Q9SE94.
MaizeGDB	146840.
Enzyme and pathway databases
BRENDA	1.5.1.20. 289.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR1_MAIZE

Accession

Primary (citable) accession number: Q9SE94

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)