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Q9SE94

- MTHR1_MAIZE

UniProt

Q9SE94 - MTHR1_MAIZE

Protein

Methylenetetrahydrofolate reductase 1

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211Proton donor/acceptorBy similarity
    Binding sitei81 – 811FADBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei175 – 1751FADBy similarity
    Binding sitei182 – 1821FADBy similarity
    Binding sitei193 – 1931SubstrateBy similarity
    Binding sitei285 – 2851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 266NADBy similarity
    Nucleotide bindingi52 – 532NAD and FADBy similarity
    Nucleotide bindingi111 – 1133FADBy similarity
    Nucleotide bindingi157 – 1604FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine metabolic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
    Alternative name(s):
    ZmMTHFR1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ9SE94.
    MaizeGDBi146840.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 593593Methylenetetrahydrofolate reductase 1PRO_0000190251Add
    BLAST

    Proteomic databases

    PRIDEiQ9SE94.
    ProMEXiQ9SE94.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SE94.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000246234.
    KOiK00297.
    OMAiYLEFFVS.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9SE94-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVIEKILEA AGDGRTAFSF EYFPPKTEEG VENLFERMDR MVAHGPSFCD    50
    ITWGAGGSTA DLTLEIANRM QNMVCVETMM HLTCTNMPVE KIDHALETIK 100
    SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVQHIRA KYGDYFGITV 150
    AGYPEAHPDA IQGEGGATLE AYSNDLAYLK RKVDAGADLI VTQLFYDTDI 200
    FLKFVNDCRQ IGITCPIVPG IMPINNYKGF LRMTGFCKTK IPSEITAALD 250
    PIKDNEEAVR QYGIHLGTEM CKKILATGIK TLHLYTLNMD KSAIGILMNL 300
    GLIEESKVSR PLPWRPATNV FRVKEDVRPI FWANRPKSYL KRTLGWDQYP 350
    HGRWGDSRNP SYGALTDHQF TRPRGRGKKL QEEWAVPLKS VEDISERFTN 400
    FCQGKLTSSP WSELDGLQPE TKIIDDQLVN INQKGFLTIN SQPAVNGEKS 450
    DSPTVGWGGP GGYVYQKAYL EFFCAKEKLD QLIEKIKAFP SLTYIAVNKD 500
    GETFSNISPN AVNAVTWGVF PGKEIIQPTV VDHASFMVWK DEAFEIWTRG 550
    WGCMFPEGDS SRELLEKVQK TYYLVSLVDN DYVQGDLFAA FKI 593
    Length:593
    Mass (Da):66,429
    Last modified:May 1, 2000 - v1
    Checksum:i4568996775B638B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174486 mRNA. Translation: AAD51733.1.
    RefSeqiNP_001104947.1. NM_001111477.1.
    UniGeneiZm.475.

    Genome annotation databases

    GeneIDi541794.
    KEGGizma:541794.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174486 mRNA. Translation: AAD51733.1 .
    RefSeqi NP_001104947.1. NM_001111477.1.
    UniGenei Zm.475.

    3D structure databases

    ProteinModelPortali Q9SE94.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9SE94.
    ProMEXi Q9SE94.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 541794.
    KEGGi zma:541794.

    Organism-specific databases

    Gramenei Q9SE94.
    MaizeGDBi 146840.

    Phylogenomic databases

    HOGENOMi HOG000246234.
    KOi K00297.
    OMAi YLEFFVS.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
      Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
      J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION.

    Entry informationi

    Entry nameiMTHR1_MAIZE
    AccessioniPrimary (citable) accession number: Q9SE94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3