Reviewed, UniProtKB/Swiss-Prot Q9SE83 (DRP2A_ARATH)

Names and origin

Protein names

Recommended name:
    Dynamin-2A
    EC=3.6.5.5
Alternative name(s):
    Dynamin-related protein 2A
    Dynamin-like protein 6

Gene names

Name:	DRP2A
Synonyms:	ADL6
Ordered Locus Names:	At1g10290
ORF Names:	F14N23.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-associated force-producing protein involved in clathrin-mediated vesicle trafficking from the trans-Golgi network to the central vacuole. Able to bind and hydrolyze GTP. Binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P). Ref.1 Ref.4 Ref.5
Catalytic activity	GTP + H2O = GDP + phosphate.
Subunit structure	Binds PtdIns3P. Interacts with SH3P3 protein. May homooligomerize or heterooligomerize. Ref.5 Ref.7
Subcellular location	Cytoplasm › cytosol. Golgi apparatus membrane; Peripheral membrane protein. Cytoplasm › cytoskeleton › phragmoplast. Note: Localized in the forming cell plate during cytokinesis. Ref.1 Ref.7
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.
Sequence caution	The sequence AAD32879.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Membrane Microtubule
Domain	Coiled coil
Ligand	GTP-binding Lipid-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Ref.1 Inferred from mutant phenotype. Source: TAIR
Cellular component	Golgi apparatus Ref.1 Inferred from direct assay. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW phragmoplast Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro lipid binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 914	914	Dynamin-2A		PRO_0000206582
Regions
	Domain	572 – 696	125	PH
	Domain	730 – 823	94	GED
	Nucleotide binding	45 – 52	8	GTP Potential
	Nucleotide binding	143 – 147	5	GTP By similarity
	Nucleotide binding	204 – 207	4	GTP By similarity
	Coiled coil	781 – 805	25	Potential
	Compositional bias	879 – 914	36	Pro-rich
Amino acid modifications
	Modified residue	533	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	51	1	K → E: Affects the trafficking between the Golgi apparatus and the vacuole. Ref.1
	Sequence conflict	617	1	T → S in BAD95292. Ref.3
	Sequence conflict	622	1	E → V in BAF00882. Ref.3
	Sequence conflict	633	1	V → G in BAD95292. Ref.3
	Sequence conflict	840	1	A → T in AAF22291. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9SE83-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: B643C7F1900A2227	FASTA	914	99,166
	10 20 30 40 50 60 MEAIDELSQL SDSMKQAASL LADEDPDETS SSKRPATFLN VVALGNVGAG KSAVLNSLIG 70 80 90 100 110 120 HPVLPTGENG ATRAPIIIEL SRESSLSSKA IILQIDNKSQ QVSASALRHS LQDRLSKGAS 130 140 150 160 170 180 GKNRDEINLK LRTSTAPPLK LVDLPGLDQR IVDESMIAEY AQHNDAILLV IVPASQASEI 190 200 210 220 230 240 SSSRALKIAK EYDPESTRTI GIIGKIDQAA ENSKALAAVQ ALLSNQGPPK TTDIPWVAVI 250 260 270 280 290 300 GQSVSIASAQ SGSGENSLET AWRAESESLK SILTGAPQSK LGRIALVDTL ASQIRSRMKL 310 320 330 340 350 360 RLPSVLSGLQ GKSQIVQDEL ARLGEQLVNS AEGTRAIALE LCREFEDKFL LHLAGGEGSG 370 380 390 400 410 420 WKVVASFEGN FPNRIKQLPL DRHFDLNNVK RVVLEADGYQ PYLISPEKGL RSLIKIVLEL 430 440 450 460 470 480 AKDPARLCVD EVHRVLVDIV SASANATPGL GRYPPFKREV VAIASAALDG FKNEAKKMVV 490 500 510 520 530 540 ALVDMERAFV PPQHFIRLVQ RRMERQRREE ELKGRSSKKG QDAEQSLLSR ATSPQPDGPT 550 560 570 580 590 600 AGGSLKSMKD KPSPQDKETP EVSGLKTAGP EGEITAGYLM KKSAKTNGWS RRWFVLNEKT 610 620 630 640 650 660 GKLGYTKKQE ERNFRGTITL EECTIEEIPE DEVEKSKSSK DKKANGPDSK GPGLVFKITC 670 680 690 700 710 720 KVPYKTVLKA HNALVLKAES VVDKNEWINK LQKVIQARGG QVGSVSMRQS LSEGSLDKMV 730 740 750 760 770 780 RKPIDPEEEL RWMSQEVRGY VEAVLNSLAA NVPKAVVLCQ VEKAKEDMLN QLYSSISAIG 790 800 810 820 830 840 NERIESLIQE DQNVKRRRER YQKQSSLLSK LTRQLSIHDN RAAAASSYSD NSGTESSPRA 850 860 870 880 890 900 SGGSSGDDWM NAFNSAANGP SDSLSKYGSG GHSRRYSDPA QNGDAASPGS GSNRRTTPNR 910 LPPAPPPTGS AYRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis." Jin J.B., Kim Y.A., Kim S.J., Lee S.H., Kim D.H., Cheong G.-W., Hwang I. Plant Cell 13:1511-1526(2001) [PubMed: 11449048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-51.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The intermolecular interaction between the PH domain and the C-terminal domain of Arabidopsis dynamin-like 6 determines lipid binding specificity." Lee S.H., Jin J.B., Song J., Min M.K., Park D.S., Kim Y.-W., Hwang I. J. Biol. Chem. 277:31842-31849(2002) [PubMed: 12105222] [Abstract] Cited for: FUNCTION, LIPID-BINDING.
[5]	"Regulation of ADL6 activity by its associated molecular network." Lam B.C.-H., Sage T.L., Bianchi F., Blumwald E. Plant J. 31:565-576(2002) [PubMed: 12207647] [Abstract] Cited for: FUNCTION, INTERACTION WITH SH3P3.
[6]	"A unified nomenclature for Arabidopsis dynamin-related large GTPases based on homology and possible functions." Hong Z., Bednarek S.Y., Blumwald E., Hwang I., Jurgens G., Menzel D., Osteryoung K.W., Raikhel N.V., Shinozaki K., Tsutsumi N., Verma D.P.S. Plant Mol. Biol. 53:261-265(2003) [PubMed: 14750516] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Phragmoplastin dynamics: multiple forms, microtubule association and their roles in cell plate formation in plants." Hong Z., Geisler-Lee C.J., Zhang Z., Verma D.P.S. Plant Mol. Biol. 53:297-312(2003) [PubMed: 14750520] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION.
[8]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-533, MASS SPECTROMETRY. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF180732 mRNA. Translation: AAF22291.1. AC005489 Genomic DNA. Translation: AAD32879.1. Sequence problems. AK221193 mRNA. Translation: BAD95292.1. AK228995 mRNA. Translation: BAF00882.1.
IPI	IPI00539942.
PIR	B86237.
RefSeq	NP_172500.1.
UniGene	At.11881 Rra.24052
3D structure databases
SMR	Q9SE83. Positions 40-312, 574-697.
ModBase	Search...
Proteomic databases
PRIDE	Q9SE83.
Genome annotation databases
GeneID	837568.
GenomeReviews	Gene locus AT1G10290 in contig CT485782_GR.
KEGG	ath:AT1G10290.
NMPDR	fig|3702.1.peg.1266.
Organism-specific databases
GeneFarm	4740. 463.
TAIR	At1g10290.
Phylogenomic databases
eggNOG	KOG0446.
HOGENOM	HBG593279.
InParanoid	Q9SE83.
OMA	MINEYAG.
PhylomeDB	Q9SE83.
Enzyme and pathway databases
BRENDA	3.6.5.5. 302.
Gene expression databases
ArrayExpress	Q9SE83.
Genevestigator	Q9SE83.
GermOnline	AT1G10290. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. IPR020850. GTPase_effector_domain_GED. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. PF00169. PH. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRP2A_ARATH

Accession

Primary (citable) accession number: Q9SE83
Secondary accession number(s): Q0WPR7, Q56YX7, Q9SY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)