Q9SE60 (MTHR1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate reductase 1
Short name=AtMTHFR1
EC=1.5.1.20

Gene names

Name:	MTHFR1
Ordered Locus Names:	At3g59970
ORF Names:	F24G16.240

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch. Ref.1
Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine. Ref.1
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SE60-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SE60-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-421: KFKELCIGNLKSSPWSELDGLQPET → VGFTLRTLVQIVSISFPHTHLHIFM
     422-592: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Methylenetetrahydrofolate reductase 1		PRO_0000190249
Natural variations
	Alternative sequence	397 – 421	25	KFKEL…LQPET → VGFTLRTLVQIVSISFPHTH LHIFM in isoform 2.		VSP_018093
	Alternative sequence	422 – 592	171	Missing in isoform 2.		VSP_018094

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 72D7453AF1AF1573	FASTA	592	66,288
	10 20 30 40 50 60 MKVVDKIKSV TEQGQTAFSF EFFPPKTEDG VENLFERMDR LVSYGPTFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLEIASRM QNVICVETMM HLTCTNMPIE KIDHALETIR SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVEGGFAC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IEADGLATPE SYQSDLAYLK 190 200 210 220 230 240 KKVDAGADLI VTQLFYDTDI FLKFVNDCRQ IGINCPIVPG IMPISNYKGF LRMAGFCKTK 250 260 270 280 290 300 IPAELTAALE PIKDNDEAVK AYGIHFATEM CKKILAHGIT SLHLYTLNVD KSAIGILMNL 310 320 330 340 350 360 GLIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWNDFP HGRWGDSHSA 370 380 390 400 410 420 AYSTLSDYQF ARPKGRDKKL QQEWVVPLKS IEDVQEKFKE LCIGNLKSSP WSELDGLQPE 430 440 450 460 470 480 TKIINEQLGK INSNGFLTIN SQPSVNAAKS DSPAIGWGGP GGYVYQKAYL EFFCSKDKLD 490 500 510 520 530 540 TLVEKSKAFP SITYMAVNKS ENWVSNTGES DVNAVTWGVF PAKEVIQPTI VDPASFKVWK 550 560 570 580 590 DEAFEIWSRS WANLYPEDDP SRKLLEEVKN SYYLVSLVDN NYINGDIFSV FA
	Isoform 2 [UniParc]. Checksum: D53A9DAEE1233C18	FASTA	421	47,258
	10 20 30 40 50 60 MKVVDKIKSV TEQGQTAFSF EFFPPKTEDG VENLFERMDR LVSYGPTFCD ITWGAGGSTA 70 80 90 100 110 120 DLTLEIASRM QNVICVETMM HLTCTNMPIE KIDHALETIR SNGIQNVLAL RGDPPHGQDK 130 140 150 160 170 180 FVQVEGGFAC ALDLVNHIRS KYGDYFGITV AGYPEAHPDV IEADGLATPE SYQSDLAYLK 190 200 210 220 230 240 KKVDAGADLI VTQLFYDTDI FLKFVNDCRQ IGINCPIVPG IMPISNYKGF LRMAGFCKTK 250 260 270 280 290 300 IPAELTAALE PIKDNDEAVK AYGIHFATEM CKKILAHGIT SLHLYTLNVD KSAIGILMNL 310 320 330 340 350 360 GLIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWNDFP HGRWGDSHSA 370 380 390 400 410 420 AYSTLSDYQF ARPKGRDKKL QQEWVVPLKS IEDVQEVGFT LRTLVQIVSI SFPHTHLHIF M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants." Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D. J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, ENZYME REGULATION.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF181966 mRNA. Translation: AAD55787.1. AL138647 Genomic DNA. Translation: CAB75816.1. CP002686 Genomic DNA. Translation: AEE79992.1. CP002686 Genomic DNA. Translation: AEE79994.1. AY122922 mRNA. Translation: AAM67455.1. AY070034 mRNA. Translation: AAL49791.1.
IPI	IPI00534913. IPI00544875.
PIR	T47821.
RefSeq	NP_191556.1. NM_115860.3. NP_850723.1. NM_180392.1.
UniGene	At.1168. At.23509. At.69007. At.72842.
3D structure databases
ProteinModelPortal	Q9SE60.
SMR	Q9SE60. Positions 1-302.
ModBase	Search...
Proteomic databases
PaxDb	Q9SE60.
PRIDE	Q9SE60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G59970.3; AT3G59970.3; AT3G59970.
GeneID	825167.
KEGG	ath:AT3G59970.
Organism-specific databases
GeneFarm	5157. 490.
TAIR	At3g59970.
Phylogenomic databases
eggNOG	COG0685.
HOGENOM	HOG000246234.
InParanoid	Q9SE60.
KO	K00297.
OMA	VVGWGPS.
PhylomeDB	Q9SE60.
ProtClustDB	PLN02540.
Enzyme and pathway databases
UniPathway	UPA00193.
Gene expression databases
ArrayExpress	Q9SE60.
Genevestigator	Q9SE60.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit. TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR1_ARATH

Accession

Primary (citable) accession number: Q9SE60
Secondary accession number(s): Q3EAG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program