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Q9SE60

- MTHR1_ARATH

UniProt

Q9SE60 - MTHR1_ARATH

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Protein

Methylenetetrahydrofolate reductase 1

Gene

MTHFR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD.By similarity

Enzyme regulationi

Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donor/acceptorBy similarity
Binding sitei81 – 811FADBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei175 – 1751FADBy similarity
Binding sitei182 – 1821FADBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei285 – 2851SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 266NADBy similarity
Nucleotide bindingi52 – 532NAD and FADBy similarity
Nucleotide bindingi111 – 1133FADBy similarity
Nucleotide bindingi157 – 1604FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciARA:AT3G59970-MONOMER.
ARA:GQT-1418-MONOMER.
ARA:GQT-258-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Short name:
AtMTHFR1
Gene namesi
Name:MTHFR1
Ordered Locus Names:At3g59970
ORF Names:F24G16.240
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G59970.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Methylenetetrahydrofolate reductase 1PRO_0000190249Add
BLAST

Proteomic databases

PaxDbiQ9SE60.
PRIDEiQ9SE60.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SE60. baseline.
GenevestigatoriQ9SE60.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9SE60.
SMRiQ9SE60. Positions 1-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiQ9SE60.
KOiK00297.
OMAiYLEFFVS.
PhylomeDBiQ9SE60.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SE60-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVVDKIKSV TEQGQTAFSF EFFPPKTEDG VENLFERMDR LVSYGPTFCD
60 70 80 90 100
ITWGAGGSTA DLTLEIASRM QNVICVETMM HLTCTNMPIE KIDHALETIR
110 120 130 140 150
SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVNHIRS KYGDYFGITV
160 170 180 190 200
AGYPEAHPDV IEADGLATPE SYQSDLAYLK KKVDAGADLI VTQLFYDTDI
210 220 230 240 250
FLKFVNDCRQ IGINCPIVPG IMPISNYKGF LRMAGFCKTK IPAELTAALE
260 270 280 290 300
PIKDNDEAVK AYGIHFATEM CKKILAHGIT SLHLYTLNVD KSAIGILMNL
310 320 330 340 350
GLIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWNDFP
360 370 380 390 400
HGRWGDSHSA AYSTLSDYQF ARPKGRDKKL QQEWVVPLKS IEDVQEKFKE
410 420 430 440 450
LCIGNLKSSP WSELDGLQPE TKIINEQLGK INSNGFLTIN SQPSVNAAKS
460 470 480 490 500
DSPAIGWGGP GGYVYQKAYL EFFCSKDKLD TLVEKSKAFP SITYMAVNKS
510 520 530 540 550
ENWVSNTGES DVNAVTWGVF PAKEVIQPTI VDPASFKVWK DEAFEIWSRS
560 570 580 590
WANLYPEDDP SRKLLEEVKN SYYLVSLVDN NYINGDIFSV FA
Length:592
Mass (Da):66,288
Last modified:May 1, 2000 - v1
Checksum:i72D7453AF1AF1573
GO
Isoform 2 (identifier: Q9SE60-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-421: KFKELCIGNLKSSPWSELDGLQPET → VGFTLRTLVQIVSISFPHTHLHIFM
     422-592: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Show »
Length:421
Mass (Da):47,258
Checksum:iD53A9DAEE1233C18
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei397 – 42125KFKEL…LQPET → VGFTLRTLVQIVSISFPHTH LHIFM in isoform 2. CuratedVSP_018093Add
BLAST
Alternative sequencei422 – 592171Missing in isoform 2. CuratedVSP_018094Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF181966 mRNA. Translation: AAD55787.1.
AL138647 Genomic DNA. Translation: CAB75816.1.
CP002686 Genomic DNA. Translation: AEE79992.1.
CP002686 Genomic DNA. Translation: AEE79994.1.
AY122922 mRNA. Translation: AAM67455.1.
AY070034 mRNA. Translation: AAL49791.1.
PIRiT47821.
RefSeqiNP_191556.1. NM_115860.3. [Q9SE60-1]
NP_850723.1. NM_180392.1. [Q9SE60-2]
UniGeneiAt.1168.
At.23509.
At.69007.
At.72842.

Genome annotation databases

EnsemblPlantsiAT3G59970.3; AT3G59970.3; AT3G59970. [Q9SE60-1]
GeneIDi825167.
KEGGiath:AT3G59970.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF181966 mRNA. Translation: AAD55787.1 .
AL138647 Genomic DNA. Translation: CAB75816.1 .
CP002686 Genomic DNA. Translation: AEE79992.1 .
CP002686 Genomic DNA. Translation: AEE79994.1 .
AY122922 mRNA. Translation: AAM67455.1 .
AY070034 mRNA. Translation: AAL49791.1 .
PIRi T47821.
RefSeqi NP_191556.1. NM_115860.3. [Q9SE60-1 ]
NP_850723.1. NM_180392.1. [Q9SE60-2 ]
UniGenei At.1168.
At.23509.
At.69007.
At.72842.

3D structure databases

ProteinModelPortali Q9SE60.
SMRi Q9SE60. Positions 1-302.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SE60.
PRIDEi Q9SE60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G59970.3 ; AT3G59970.3 ; AT3G59970 . [Q9SE60-1 ]
GeneIDi 825167.
KEGGi ath:AT3G59970.

Organism-specific databases

GeneFarmi 5157. 490.
TAIRi AT3G59970.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
InParanoidi Q9SE60.
KOi K00297.
OMAi YLEFFVS.
PhylomeDBi Q9SE60.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci ARA:AT3G59970-MONOMER.
ARA:GQT-1418-MONOMER.
ARA:GQT-258-MONOMER.

Gene expression databases

ExpressionAtlasi Q9SE60. baseline.
Genevestigatori Q9SE60.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
    Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
    J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, ENZYME REGULATION.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.

Entry informationi

Entry nameiMTHR1_ARATH
AccessioniPrimary (citable) accession number: Q9SE60
Secondary accession number(s): Q3EAG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3