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Q9SE60

- MTHR1_ARATH

UniProt

Q9SE60 - MTHR1_ARATH

Protein

Methylenetetrahydrofolate reductase 1

Gene

MTHFR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    The probable reversibility of the MTHFR reaction in plants suggests that they can metabolize the methyl group of 5,10-methylenetetrahydrofolate to serine, sugars and starch.1 Publication

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Plant MTHFRs strongly prefer NADH over NADPH. Not inhibited by methionine or S-adenosylmethionine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211Proton donor/acceptorBy similarity
    Binding sitei81 – 811FADBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei175 – 1751FADBy similarity
    Binding sitei182 – 1821FADBy similarity
    Binding sitei193 – 1931SubstrateBy similarity
    Binding sitei285 – 2851SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 266NADBy similarity
    Nucleotide bindingi52 – 532NAD and FADBy similarity
    Nucleotide bindingi111 – 1133FADBy similarity
    Nucleotide bindingi157 – 1604FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciARA:AT3G59970-MONOMER.
    ARA:GQT-1418-MONOMER.
    ARA:GQT-258-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
    Short name:
    AtMTHFR1
    Gene namesi
    Name:MTHFR1
    Ordered Locus Names:At3g59970
    ORF Names:F24G16.240
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G59970.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 592592Methylenetetrahydrofolate reductase 1PRO_0000190249Add
    BLAST

    Proteomic databases

    PaxDbiQ9SE60.
    PRIDEiQ9SE60.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9SE60.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SE60.
    SMRiQ9SE60. Positions 1-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    InParanoidiQ9SE60.
    KOiK00297.
    OMAiYLEFFVS.
    PhylomeDBiQ9SE60.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.
    TIGR00677. fadh2_euk. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9SE60-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKVVDKIKSV TEQGQTAFSF EFFPPKTEDG VENLFERMDR LVSYGPTFCD    50
    ITWGAGGSTA DLTLEIASRM QNVICVETMM HLTCTNMPIE KIDHALETIR 100
    SNGIQNVLAL RGDPPHGQDK FVQVEGGFAC ALDLVNHIRS KYGDYFGITV 150
    AGYPEAHPDV IEADGLATPE SYQSDLAYLK KKVDAGADLI VTQLFYDTDI 200
    FLKFVNDCRQ IGINCPIVPG IMPISNYKGF LRMAGFCKTK IPAELTAALE 250
    PIKDNDEAVK AYGIHFATEM CKKILAHGIT SLHLYTLNVD KSAIGILMNL 300
    GLIDESKISR SLPWRRPANV FRTKEDVRPI FWANRPKSYI SRTKGWNDFP 350
    HGRWGDSHSA AYSTLSDYQF ARPKGRDKKL QQEWVVPLKS IEDVQEKFKE 400
    LCIGNLKSSP WSELDGLQPE TKIINEQLGK INSNGFLTIN SQPSVNAAKS 450
    DSPAIGWGGP GGYVYQKAYL EFFCSKDKLD TLVEKSKAFP SITYMAVNKS 500
    ENWVSNTGES DVNAVTWGVF PAKEVIQPTI VDPASFKVWK DEAFEIWSRS 550
    WANLYPEDDP SRKLLEEVKN SYYLVSLVDN NYINGDIFSV FA 592
    Length:592
    Mass (Da):66,288
    Last modified:May 1, 2000 - v1
    Checksum:i72D7453AF1AF1573
    GO
    Isoform 2 (identifier: Q9SE60-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         397-421: KFKELCIGNLKSSPWSELDGLQPET → VGFTLRTLVQIVSISFPHTHLHIFM
         422-592: Missing.

    Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:421
    Mass (Da):47,258
    Checksum:iD53A9DAEE1233C18
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei397 – 42125KFKEL…LQPET → VGFTLRTLVQIVSISFPHTH LHIFM in isoform 2. CuratedVSP_018093Add
    BLAST
    Alternative sequencei422 – 592171Missing in isoform 2. CuratedVSP_018094Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF181966 mRNA. Translation: AAD55787.1.
    AL138647 Genomic DNA. Translation: CAB75816.1.
    CP002686 Genomic DNA. Translation: AEE79992.1.
    CP002686 Genomic DNA. Translation: AEE79994.1.
    AY122922 mRNA. Translation: AAM67455.1.
    AY070034 mRNA. Translation: AAL49791.1.
    PIRiT47821.
    RefSeqiNP_191556.1. NM_115860.3. [Q9SE60-1]
    NP_850723.1. NM_180392.1. [Q9SE60-2]
    UniGeneiAt.1168.
    At.23509.
    At.69007.
    At.72842.

    Genome annotation databases

    EnsemblPlantsiAT3G59970.3; AT3G59970.3; AT3G59970. [Q9SE60-1]
    GeneIDi825167.
    KEGGiath:AT3G59970.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF181966 mRNA. Translation: AAD55787.1 .
    AL138647 Genomic DNA. Translation: CAB75816.1 .
    CP002686 Genomic DNA. Translation: AEE79992.1 .
    CP002686 Genomic DNA. Translation: AEE79994.1 .
    AY122922 mRNA. Translation: AAM67455.1 .
    AY070034 mRNA. Translation: AAL49791.1 .
    PIRi T47821.
    RefSeqi NP_191556.1. NM_115860.3. [Q9SE60-1 ]
    NP_850723.1. NM_180392.1. [Q9SE60-2 ]
    UniGenei At.1168.
    At.23509.
    At.69007.
    At.72842.

    3D structure databases

    ProteinModelPortali Q9SE60.
    SMRi Q9SE60. Positions 1-302.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SE60.
    PRIDEi Q9SE60.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G59970.3 ; AT3G59970.3 ; AT3G59970 . [Q9SE60-1 ]
    GeneIDi 825167.
    KEGGi ath:AT3G59970.

    Organism-specific databases

    GeneFarmi 5157. 490.
    TAIRi AT3G59970.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    InParanoidi Q9SE60.
    KOi K00297.
    OMAi YLEFFVS.
    PhylomeDBi Q9SE60.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci ARA:AT3G59970-MONOMER.
    ARA:GQT-1418-MONOMER.
    ARA:GQT-258-MONOMER.

    Gene expression databases

    Genevestigatori Q9SE60.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and functional expression of cDNAs encoding NADH-dependent methylenetetrahydrofolate reductase from higher plants."
      Roje S., Wang H., McNeil S.D., Raymond R.K., Appling D.R., Shachar-Hill Y., Bohnert H.J., Hanson A.D.
      J. Biol. Chem. 274:36089-36096(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, ENZYME REGULATION.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMTHR1_ARATH
    AccessioniPrimary (citable) accession number: Q9SE60
    Secondary accession number(s): Q3EAG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3