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Protein

Beta-D-glucopyranosyl abscisate beta-glucosidase

Gene

BGLU18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes abscisic acid glucose ester (ABA-GE) which represents the predominant form of conjugated ABA (biologically inactive). No activity with beta-D-glucopyranosyl zeatin. The hydrolysis of ABA-GE in the endoplasmic reticulum (ER) forms free ABA and contributes to increase its cellular levels under dehydration conditions. ABA-GE hydrolyzing activity is enhanced by dehydration stress-induced polymerization into higher molecular weight forms. The ABA produced by BGLU18 contributes to the initiation of intracellular signaling as well as the increase in the extracellular ABA level.2 Publications

Miscellaneous

Polymerization into higher molecular weight forms displays diurnal fluctuation, similar to the ABA level.

Catalytic activityi

D-glucopyranosyl abscisate + H2O = D-glucose + abscisate.1 Publication

Kineticsi

  1. KM=0.41 mM for ABA-beta-D-glucopyranosyl ester1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei58SubstrateBy similarity1
    Binding sitei161SubstrateBy similarity1
    Binding sitei206SubstrateBy similarity1
    Active sitei207Proton donorCurated1
    Binding sitei351SubstrateBy similarity1
    Active sitei422NucleophileBy similarity1
    Binding sitei473SubstrateBy similarity1

    GO - Molecular functioni

    • abscisic acid glucose ester beta-glucosidase activity Source: TAIR
    • beta-glucosidase activity Source: UniProtKB
    • identical protein binding Source: TAIR

    GO - Biological processi

    • abscisic acid-activated signaling pathway Source: UniProtKB-KW
    • abscisic acid metabolic process Source: TAIR
    • carbohydrate metabolic process Source: InterPro
    • defense response to fungus Source: TAIR
    • glycosyl compound metabolic process Source: GO_Central
    • positive regulation of abscisic acid-activated signaling pathway Source: UniProtKB
    • protein polymerization Source: UniProtKB
    • regulation of stomatal movement Source: UniProtKB
    • response to abscisic acid Source: UniProtKB
    • response to insect Source: TAIR
    • response to salt stress Source: TAIR
    • response to water deprivation Source: TAIR
    • water homeostasis Source: UniProtKB

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processAbscisic acid signaling pathway

    Enzyme and pathway databases

    BioCyciARA:AT1G52400-MONOMER
    MetaCyc:AT1G52400-MONOMER
    BRENDAi3.2.1.175 399
    ReactomeiR-ATH-189085 Digestion of dietary carbohydrate

    Protein family/group databases

    CAZyiGH1 Glycoside Hydrolase Family 1

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-glucopyranosyl abscisate beta-glucosidase (EC:3.2.1.175)
    Alternative name(s):
    Beta-glucosidase 1
    Short name:
    AtBG1
    Beta-glucosidase 18
    Short name:
    AtBGLU18
    Beta-glucosidase homolog 1
    Gene namesi
    Name:BGLU18
    Synonyms:BG1, BGL1
    Ordered Locus Names:At1g52400
    ORF Names:F19K6.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G52400
    TAIRilocus:2018179 AT1G52400

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Reduced growth, yellow leaf, defective stomatal closure in the dark, increased transpirational water loss, sensitive response to dehydration, lower germination efficiency and decreased ABA levels.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi207E → Q: Loss of activity towards ABA-GE. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 26Sequence analysisAdd BLAST26
    ChainiPRO_000001176627 – 528Beta-D-glucopyranosyl abscisate beta-glucosidaseAdd BLAST502

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi226 ↔ 237By similarity
    Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi499N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9SE50
    PRIDEiQ9SE50

    2D gel databases

    SWISS-2DPAGEiQ9SE50

    Expressioni

    Tissue specificityi

    Seeds and hydathodes of rosette and cauline leaves.1 Publication

    Inductioni

    By ABA, dehydration, wounding and salt treatment.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9SE50 baseline and differential
    GenevisibleiQ9SE50 AT

    Interactioni

    GO - Molecular functioni

    • identical protein binding Source: TAIR

    Protein-protein interaction databases

    BioGridi26895, 3 interactors
    STRINGi3702.AT1G52400.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SE50
    SMRiQ9SE50
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni480 – 481Substrate bindingBy similarity2

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi525 – 528Prevents secretion from ERPROSITE-ProRule annotation4

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG0626 Eukaryota
    COG2723 LUCA
    HOGENOMiHOG000088630
    InParanoidiQ9SE50
    KOiK15748
    OMAiCENHNAD
    OrthoDBiEOG0936063X
    PhylomeDBiQ9SE50

    Family and domain databases

    InterProiView protein in InterPro
    IPR001360 Glyco_hydro_1
    IPR033132 Glyco_hydro_1_N_CS
    IPR017853 Glycoside_hydrolase_SF
    PANTHERiPTHR10353 PTHR10353, 1 hit
    PfamiView protein in Pfam
    PF00232 Glyco_hydro_1, 1 hit
    PRINTSiPR00131 GLHYDRLASE1
    SUPFAMiSSF51445 SSF51445, 1 hit
    PROSITEiView protein in PROSITE
    PS00014 ER_TARGET, 1 hit
    PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9SE50-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVRFEKVHLV LGLALVLTLV GAPTKAQGPV CGAGLPDKFS RLNFPEGFIW
    60 70 80 90 100
    GTATAAFQVE GAVNEGCRGP SMWDTFTKKF PHRCENHNAD VAVDFYHRYK
    110 120 130 140 150
    EDIQLMKDLN TDAFRLSIAW PRIFPHGRMS KGISKVGVQF YHDLIDELLK
    160 170 180 190 200
    NNIIPLVTVF HWDTPQDLED EYGGFLSGRI VQDFTEYANF TFHEYGHKVK
    210 220 230 240 250
    HWITFNEPWV FSRAGYDNGK KAPGRCSPYI PGYGQHCQDG RSGYEAYQVS
    260 270 280 290 300
    HNLLLSHAYA VDAFRNCKQC AGGKIGIAHS PAWFEPQDLE HVGGSIERVL
    310 320 330 340 350
    DFILGWHLAP TTYGDYPQSM KDRVGHRLPK FTEAEKKLLK GSTDYVGMNY
    360 370 380 390 400
    YTSVFAKEIS PDPKSPSWTT DSLVDWDSKS VDGYKIGSKP FNGKLDVYSK
    410 420 430 440 450
    GLRYLLKYIK DNYGDPEVII AENGYGEDLG EKHNDVNFGT QDHNRKYYIQ
    460 470 480 490 500
    RHLLSMHDAI CKDKVNVTGY FVWSLMDNFE WQDGYKARFG LYYIDFQNNL
    510 520
    TRHQKVSGKW YSEFLKPQFP TSKLREEL
    Length:528
    Mass (Da):60,459
    Last modified:February 5, 2008 - v2
    Checksum:iF148D04C0A6F27B8
    GO
    Isoform 2 (identifier: Q9SE50-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         461-461: C → W
         462-528: Missing.

    Note: Derived from EST data. No experimental confirmation available.
    Show »
    Length:461
    Mass (Da):52,371
    Checksum:iA83D86C9F943776D
    GO

    Sequence cautioni

    The sequence BAD94819 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti134S → N in AAF22295 (Ref. 2) Curated1
    Sequence conflicti210V → G in AAN60329 (Ref. 6) Curated1
    Sequence conflicti214A → V in AAN60329 (Ref. 6) Curated1
    Sequence conflicti253L → S in AAN31804 (Ref. 6) Curated1
    Sequence conflicti266N → K in CAC19786 (PubMed:11080278).Curated1
    Sequence conflicti266N → K in AAN60329 (Ref. 6) Curated1
    Sequence conflicti365S → N in CAC19786 (PubMed:11080278).Curated1
    Sequence conflicti431E → G in BAD94819 (Ref. 7) Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_041996461C → W in isoform 2. Curated1
    Alternative sequenceiVSP_041997462 – 528Missing in isoform 2. CuratedAdd BLAST67

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ251301 mRNA Translation: CAC19786.1
    AF183827 mRNA Translation: AAF22295.1
    AC037424 Genomic DNA Translation: AAG51546.1
    CP002684 Genomic DNA Translation: AEE32799.1
    CP002684 Genomic DNA Translation: AEE32800.1
    CP002684 Genomic DNA Translation: AEE32801.1
    AY039855 mRNA Translation: AAK63959.1
    AY056415 mRNA Translation: AAL08271.1
    BT000515 mRNA Translation: AAN18084.1
    BT000657 mRNA Translation: AAN31804.1
    AF083771 mRNA Translation: AAN60329.1
    AK222051 mRNA Translation: BAD94819.1 Different initiation.
    PIRiC96564
    RefSeqiNP_001031175.1, NM_001036098.2 [Q9SE50-2]
    NP_001185204.1, NM_001198275.1 [Q9SE50-1]
    NP_175649.1, NM_104118.3 [Q9SE50-1]
    UniGeneiAt.24169

    Genome annotation databases

    EnsemblPlantsiAT1G52400.1; AT1G52400.1; AT1G52400 [Q9SE50-1]
    AT1G52400.2; AT1G52400.2; AT1G52400 [Q9SE50-2]
    AT1G52400.3; AT1G52400.3; AT1G52400 [Q9SE50-1]
    GeneIDi841670
    GrameneiAT1G52400.1; AT1G52400.1; AT1G52400 [Q9SE50-1]
    AT1G52400.2; AT1G52400.2; AT1G52400 [Q9SE50-2]
    AT1G52400.3; AT1G52400.3; AT1G52400 [Q9SE50-1]
    KEGGiath:AT1G52400

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiBGL18_ARATH
    AccessioniPrimary (citable) accession number: Q9SE50
    Secondary accession number(s): F4ICX8
    , Q56WI9, Q8H169, Q8H7A3, Q93V63, Q9FNZ3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: February 5, 2008
    Last modified: April 25, 2018
    This is version 132 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health