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Protein

Isocitrate lyase

Gene

ICL

Organism
Dendrobium crumenatum (Tropical pigeon orchid)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in storage lipid mobilization during the growth of higher plant seedling.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathway: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL)
  2. no protein annotated in this organism
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi174 – 1741MagnesiumBy similarity
Active sitei212 – 2121Proton acceptorBy similarity
Binding sitei249 – 2491SubstrateBy similarity
Binding sitei471 – 4711SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitratsysaseBy similarity
Gene namesi
Name:ICL
OrganismiDendrobium crumenatum (Tropical pigeon orchid)
Taxonomic identifieri51096 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesOrchidaceaeEpidendroideaeDendrobiinaeDendrobium

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Isocitrate lyasePRO_0000068805Add
BLAST

Expressioni

Developmental stagei

Expressed maximally during postgerminative growth.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9SE26.
SMRiQ9SE26. Positions 8-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1053Substrate bindingBy similarity
Regioni213 – 2142Substrate bindingBy similarity
Regioni436 – 4405Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi572 – 5743Microbody targeting signalSequence Analysis

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SE26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSVPPMI TEEEARFEAE VSAVESWWRT DRFRLTRRPY SARDVVSLRG
60 70 80 90 100
TLHHSYASDQ MAKKLWRTLK SHQSAGTASR TFGALDPVQV TMMAKHLDTI
110 120 130 140 150
YVSGWQCSST HTATNEPGPD LADYPYNTVP NKVEHLFFAQ LYHDRKQHEA
160 170 180 190 200
RVSMTREQRA KTPYVDYLRP IIADGDTGFG GATATVKLCK LFVERGAAGV
210 220 230 240 250
HIEDQSSVTK KCGHMAGKVL VAVSEHINRL VAARLQFDVM GVETVLVART
260 270 280 290 300
DAVAATLIQS NVDLRDHQFI LGATNPDFKR RSLAAVLSAA MAAGKTGAVL
310 320 330 340 350
QAIEDDWLSR AGLMTFSDAV INGINRQNLP EYEKQRRLNE WAAATEYSKC
360 370 380 390 400
VSNEQGREIA ERLGAGEIFW DWDIARTREG FYRFRGSVEA AVVRGRAFAP
410 420 430 440 450
HADLIWMETS SPDLVECGKF AQGMKASHPE IMLAYNLSPS FNWDAAGMTD
460 470 480 490 500
EEMRDFIPRI AKMGFCWQFI TLGGFHADAL VTDTFAREFA KQGMLAYVER
510 520 530 540 550
IQREERNNGV DTLAHQKWSG ANYYDRYLKT VQGGISSTAA MGKGVTEEQF
560 570
KEESRTGTRG LDRGGITVNA KSRL
Length:574
Mass (Da):63,993
Last modified:May 1, 2000 - v1
Checksum:i0B568ADE3248DE2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF193815 mRNA. Translation: AAF04598.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF193815 mRNA. Translation: AAF04598.1.

3D structure databases

ProteinModelPortaliQ9SE26.
SMRiQ9SE26. Positions 8-534.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of DcrIcl, an isocitrate lyase gene from the tropical orchid, Dendrobium crumenatum."
    Vellupillai M., Goh C.-J., Swarup S.
    Plant Gene Register PGR99-178
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiACEA_DENCR
AccessioniPrimary (citable) accession number: Q9SE26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.