true2009-05-052023-09-1391PPAF1_IPOBABinuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean.Schenk G.Ge Y.Carrington L.E.Wynne C.J.Searle I.R.Carroll B.J.Hamilton S.de Jersey J.doi:10.1006/abbi.1999.14071999Arch. Biochem. Biophys.370183-189NUCLEOTIDE SEQUENCE [MRNA]CATALYTIC ACTIVITYCOFACTORBIOPHYSICOCHEMICAL PROPERTIEScv. GoldenPhosphate forms an unusual tripodal complex with the Fe-Mn center of sweet potato purple acid phosphatase.Schenk G.Gahan L.R.Carrington L.E.Mitic N.Valizadeh M.Hamilton S.E.de Jersey J.Guddat L.W.doi:10.1073/pnas.04072391022005Proc. Natl. Acad. Sci. U.S.A.102273-278X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 39-464 IN COMPLEX WITH SUBSTRATE AND COFACTORBIOPHYSICOCHEMICAL PROPERTIES2.50A/B=39-4644 sites, No reported glycans2773MPP_PAPsPurple acid phosphatase-like, N-terminalCalcineurin-like_PHP_ApaHMetallo-depent_PP-likeMPP_PAPPPA-likePurple_acid_Pase-like_NPurple_acid_Pase_NPurple_acid_PPase_C_domACID PHOSPHATASE RELATEDFE(3+)-ZN(2+) PURPLE ACID PHOSPHATASE 12MetallophosMetallophos_CPur_ac_phosph_NMetallo-dependent phosphatasesPurple acid phosphatase, N-terminal domainPurple acid phosphatase 13.1.3.2Manganese(II) purple acid phosphatase 1PAP1Binds 1 Fe cation per subunit.Binds 1 Mn(2+) ion per subunit. Can also use Zn(2+), Cu(2+) and Mg(2+) ions.56095 uM for p-NPP (at pH 4.9 and 25 degrees Celsius)120 uM for ATP (at pH 4.9 and 25 degrees Celsius)180 uM for ADP (at pH 4.9 and 25 degrees Celsius)360 uM for AMP (at pH 4.9 and 25 degrees Celsius)75 uM for pyrophosphate (at pH 4.9 and 25 degrees Celsius)490 uM for beta-glycerophosphate (at pH 4.9 and 25 degrees Celsius)44 uM for 4-nitrophenol phosphate (at pH 3.5)68 uM for 4-nitrophenol phosphate (at pH 4)68 uM for 4-nitrophenol phosphate (at pH 4.5)93 uM for 4-nitrophenol phosphate (at pH 5)330 uM for 4-nitrophenol phosphate (at pH 6)930 uM for 4-nitrophenol phosphate (at pH 6.5)1800 uM for 4-nitrophenol phosphate (at pH 7)5000 uM for 4-nitrophenol phosphate (at pH 8)Optimum pH is 4.5.Homodimer; disulfide-linked.Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.138Purple acid phosphatase 14970839473Proton donor333172201204238323360362N-linked (GlcNAc...) asparagine118N-linked (GlcNAc...) asparagine180N-linked (GlcNAc...) asparagine311N-linked (GlcNAc...) asparagine433Interchain3824245525464707276788689929398105108110112123129137142145147149154165170177188194198205207209211214227240245247254259265267274279282286298309312314317321332337338350354358367369371392396417421425431443452458substrate2000-05-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