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Protein

Purple acid phosphatase 1

Gene

PAP1

Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Absorptioni

Abs(max)=560 nm2 Publications

Kineticsi

  1. KM=95 µM for p-NPP (at pH 4.9 and 25 degrees Celsius)2 Publications
  2. KM=120 µM for ATP (at pH 4.9 and 25 degrees Celsius)2 Publications
  3. KM=180 µM for ADP (at pH 4.9 and 25 degrees Celsius)2 Publications
  4. KM=360 µM for AMP (at pH 4.9 and 25 degrees Celsius)2 Publications
  5. KM=75 µM for pyrophosphate (at pH 4.9 and 25 degrees Celsius)2 Publications
  6. KM=490 µM for beta-glycerophosphate (at pH 4.9 and 25 degrees Celsius)2 Publications
  7. KM=44 µM for 4-nitrophenol phosphate (at pH 3.5)2 Publications
  8. KM=68 µM for 4-nitrophenol phosphate (at pH 4)2 Publications
  9. KM=68 µM for 4-nitrophenol phosphate (at pH 4.5)2 Publications
  10. KM=93 µM for 4-nitrophenol phosphate (at pH 5)2 Publications
  11. KM=330 µM for 4-nitrophenol phosphate (at pH 6)2 Publications
  12. KM=930 µM for 4-nitrophenol phosphate (at pH 6.5)2 Publications
  13. KM=1800 µM for 4-nitrophenol phosphate (at pH 7)2 Publications
  14. KM=5000 µM for 4-nitrophenol phosphate (at pH 8)2 Publications

    pH dependencei

    Optimum pH is 4.5.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi172IronBy similarity1
    Metal bindingi201IronBy similarity1
    Metal bindingi201Manganese1
    Metal bindingi204IronBy similarity1
    Metal bindingi238Manganese1
    Binding sitei238SubstrateBy similarity1
    Metal bindingi323Manganese1
    Active sitei333Proton donorBy similarity1
    Metal bindingi360Manganese1
    Metal bindingi362IronBy similarity1

    GO - Molecular functioni

    Keywordsi

    Molecular functionHydrolase
    LigandIron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15153
    BRENDAi3.1.3.2 2773

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 1 (EC:3.1.3.2)
    Alternative name(s):
    Manganese(II) purple acid phosphatase 1
    Gene namesi
    Name:PAP1
    OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
    Taxonomic identifieri4120 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 38Sequence analysisAdd BLAST38
    ChainiPRO_500005735139 – 473Purple acid phosphatase 1Add BLAST435

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi118N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi180N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi311N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi382InterchainBy similarity
    Glycosylationi433N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9SE00

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Structurei

    Secondary structure

    1473
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi42 – 45Combined sources4
    Helixi52 – 54Combined sources3
    Beta strandi64 – 70Combined sources7
    Beta strandi72 – 76Combined sources5
    Beta strandi78 – 86Combined sources9
    Turni89 – 92Combined sources4
    Beta strandi93 – 98Combined sources6
    Beta strandi105 – 108Combined sources4
    Beta strandi110 – 112Combined sources3
    Beta strandi123 – 129Combined sources7
    Beta strandi137 – 142Combined sources6
    Helixi145 – 147Combined sources3
    Beta strandi149 – 154Combined sources6
    Beta strandi165 – 170Combined sources6
    Helixi177 – 188Combined sources12
    Beta strandi194 – 198Combined sources5
    Helixi205 – 207Combined sources3
    Helixi209 – 211Combined sources3
    Helixi214 – 227Combined sources14
    Helixi238 – 240Combined sources3
    Helixi245 – 247Combined sources3
    Helixi254 – 259Combined sources6
    Helixi265 – 267Combined sources3
    Beta strandi274 – 279Combined sources6
    Beta strandi282 – 286Combined sources5
    Helixi298 – 309Combined sources12
    Turni312 – 314Combined sources3
    Beta strandi317 – 321Combined sources5
    Turni332 – 337Combined sources6
    Helixi338 – 350Combined sources13
    Beta strandi354 – 358Combined sources5
    Beta strandi360 – 367Combined sources8
    Beta strandi369 – 371Combined sources3
    Beta strandi392 – 396Combined sources5
    Beta strandi417 – 421Combined sources5
    Beta strandi425 – 431Combined sources7
    Beta strandi433 – 443Combined sources11
    Beta strandi452 – 458Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1XZWX-ray2.50A/B39-464[»]
    ProteinModelPortaliQ9SE00
    SMRiQ9SE00
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9SE00

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni360 – 362Substrate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.380, 1 hit
    3.60.21.10, 1 hit
    InterProiView protein in InterPro
    IPR004843 Calcineurin-like_PHP_ApaH
    IPR029052 Metallo-depent_PP-like
    IPR008963 Purple_acid_Pase-like_N
    IPR015914 Purple_acid_Pase_N
    IPR025733 Purple_acid_PPase_C_dom
    PfamiView protein in Pfam
    PF00149 Metallophos, 1 hit
    PF14008 Metallophos_C, 1 hit
    PF16656 Pur_ac_phosph_N, 1 hit
    SUPFAMiSSF49363 SSF49363, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SE00-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLVVVGLWC LILGLILNPT KFCDAGVTSS YVRKSLSALP NAEDVDMPWD
    60 70 80 90 100
    SDVFAVPSGY NAPQQVHITQ GDYEGRGVII SWTTPYDKAG ANKVVYWSEN
    110 120 130 140 150
    SKSQKRAMGT VVTYKYYNYT SAFIHHCTIK DLEYDTKYYY RLGFGDAKRQ
    160 170 180 190 200
    FWFVTPPKPG PDVPYVFGLI GDIGQTHDSN TTLTHYEQNS AKGQAVLFMG
    210 220 230 240 250
    DLSYSNRWPN HDNNRWDTWG RFSERSVAYQ PWIWTAGNHE IDYAPDIGEY
    260 270 280 290 300
    QPFVPFTNRY PTPHEASGSG DPLWYAIKRA SAHIIVLSSY SGFVKYSPQY
    310 320 330 340 350
    KWFTSELEKV NRSETPWLIV LVHAPLYNSY EAHYMEGEAM RAIFEPYFVY
    360 370 380 390 400
    YKVDIVFSGH VHSYERSERV SNVAYNIVNA KCTPVSDESA PVYITIGDGG
    410 420 430 440 450
    NSEGLASEMT QPQPSYSAFR EASFGHGIFD IKNRTHAHFS WHRNQDGASV
    460 470
    EADSLWLLNR YWASEDASSM SAM
    Length:473
    Mass (Da):53,815
    Last modified:May 1, 2000 - v1
    Checksum:iBAE4B807DADD95A7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200825 mRNA Translation: AAF19821.1
    PIRiA59200

    Similar proteinsi

    Entry informationi

    Entry nameiPPAF1_IPOBA
    AccessioniPrimary (citable) accession number: Q9SE00
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 1, 2000
    Last modified: May 23, 2018
    This is version 76 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

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