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Q9SDZ9

- PPAF2_IPOBA

UniProt

Q9SDZ9 - PPAF2_IPOBA

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Protein

Purple acid phosphatase 2

Gene

PAP2

Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Absorptioni

Abs(max)=560 nm1 Publication

Kineticsi

  1. KM=95 µM for p-NPP (at pH 4.9 and 25 degrees Celsius)1 Publication
  2. KM=120 µM for ATP (at pH 4.9 and 25 degrees Celsius)1 Publication
  3. KM=180 µM for ADP (at pH 4.9 and 25 degrees Celsius)1 Publication
  4. KM=360 µM for AMP (at pH 4.9 and 25 degrees Celsius)1 Publication
  5. KM=75 µM for pyrophosphate (at pH 4.9 and 25 degrees Celsius)1 Publication
  6. KM=490 µM for beta-glycerophosphate (at pH 4.9 and 25 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi164 – 1641IronBy similarity
Metal bindingi193 – 1931IronBy similarity
Metal bindingi193 – 1931ManganeseBy similarity
Metal bindingi196 – 1961IronBy similarity
Metal bindingi230 – 2301ManganeseBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Metal bindingi315 – 3151ManganeseBy similarity
Active sitei325 – 3251Proton donorBy similarity
Metal bindingi352 – 3521ManganeseBy similarity
Metal bindingi354 – 3541IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15154.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 2 (EC:3.1.3.2)
Alternative name(s):
Manganese(II) purple acid phosphatase 2
Gene namesi
Name:PAP2
OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
Taxonomic identifieri4120 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 465433Purple acid phosphatase 2PRO_5000057352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi374 – 374InterchainBy similarity
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9SDZ9.
SMRiQ9SDZ9. Positions 39-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni352 – 3543Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SDZ9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGASRTGCYL LAVVLAAVMN AAIAGITSSF IRKVEKTVDM PLDSDVFRVP
60 70 80 90 100
PGYNAPQQVH ITQGDHVGKA MIVSWVTVDE PGSSKVVYWS ENSQHKKVAR
110 120 130 140 150
GNIRTYTYFN YTSGYIHHCT IRNLEYNTKY YYEVGIGNTT RSFWFTTPPE
160 170 180 190 200
VGPDVPYTFG LIGDLGQSFD SNRTLTHYER NPIKGQAVLF VGDLSYADNY
210 220 230 240 250
PNHDNVRWDT WGRFVERSTA YQPWIWTAGN HEIDFAPEIG ETKPFKPFTK
260 270 280 290 300
RYHVPYKASG STETFWYPIK RASAYIIVLS SYSAYGKYTP QYKWLEEELP
310 320 330 340 350
KVNRTETPWL IVLMHSPWYN SYNYHYMEGE TMRVMYEPWF VQHKVDLVFA
360 370 380 390 400
GHVHAYERSE RVSNVAYDIV NGKCTPVRDQ SAPVYITIGD GGNLEGLATN
410 420 430 440 450
MTDPQPEYSA FREASFGHAT LDIKNRTHAY YSWHRNQDGY AVEADSMWVS
460
NRFWHPVDDS TTTKL
Length:465
Mass (Da):53,401
Last modified:May 1, 2000 - v1
Checksum:i49C0DCBA1CD8DF72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200826 mRNA. Translation: AAF19822.1.
PIRiT51095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200826 mRNA. Translation: AAF19822.1 .
PIRi T51095.

3D structure databases

ProteinModelPortali Q9SDZ9.
SMRi Q9SDZ9. Positions 39-461.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15154.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean."
    Schenk G., Ge Y., Carrington L.E., Wynne C.J., Searle I.R., Carroll B.J., Hamilton S., de Jersey J.
    Arch. Biochem. Biophys. 370:183-189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Golden.

Entry informationi

Entry nameiPPAF2_IPOBA
AccessioniPrimary (citable) accession number: Q9SDZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3