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Protein

Purple acid phosphatase 2

Gene

PAP2

Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Absorptioni

Abs(max)=560 nm1 Publication

Kineticsi

  1. KM=95 µM for p-NPP (at pH 4.9 and 25 degrees Celsius)1 Publication
  2. KM=120 µM for ATP (at pH 4.9 and 25 degrees Celsius)1 Publication
  3. KM=180 µM for ADP (at pH 4.9 and 25 degrees Celsius)1 Publication
  4. KM=360 µM for AMP (at pH 4.9 and 25 degrees Celsius)1 Publication
  5. KM=75 µM for pyrophosphate (at pH 4.9 and 25 degrees Celsius)1 Publication
  6. KM=490 µM for beta-glycerophosphate (at pH 4.9 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi164IronBy similarity1
    Metal bindingi193IronBy similarity1
    Metal bindingi193ManganeseBy similarity1
    Metal bindingi196IronBy similarity1
    Metal bindingi230ManganeseBy similarity1
    Binding sitei230SubstrateBy similarity1
    Metal bindingi315ManganeseBy similarity1
    Active sitei325Proton donorBy similarity1
    Metal bindingi352ManganeseBy similarity1
    Metal bindingi354IronBy similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 2 (EC:3.1.3.2)
    Alternative name(s):
    Manganese(II) purple acid phosphatase 2
    Gene namesi
    Name:PAP2
    OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
    Taxonomic identifieri4120 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 32Sequence analysisAdd BLAST32
    ChainiPRO_500005735233 – 465Purple acid phosphatase 2Add BLAST433

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
    Disulfide bondi374InterchainBy similarity
    Glycosylationi400N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi425N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SDZ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni352 – 354Substrate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    PF16656. Pur_ac_phosph_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SDZ9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGASRTGCYL LAVVLAAVMN AAIAGITSSF IRKVEKTVDM PLDSDVFRVP
    60 70 80 90 100
    PGYNAPQQVH ITQGDHVGKA MIVSWVTVDE PGSSKVVYWS ENSQHKKVAR
    110 120 130 140 150
    GNIRTYTYFN YTSGYIHHCT IRNLEYNTKY YYEVGIGNTT RSFWFTTPPE
    160 170 180 190 200
    VGPDVPYTFG LIGDLGQSFD SNRTLTHYER NPIKGQAVLF VGDLSYADNY
    210 220 230 240 250
    PNHDNVRWDT WGRFVERSTA YQPWIWTAGN HEIDFAPEIG ETKPFKPFTK
    260 270 280 290 300
    RYHVPYKASG STETFWYPIK RASAYIIVLS SYSAYGKYTP QYKWLEEELP
    310 320 330 340 350
    KVNRTETPWL IVLMHSPWYN SYNYHYMEGE TMRVMYEPWF VQHKVDLVFA
    360 370 380 390 400
    GHVHAYERSE RVSNVAYDIV NGKCTPVRDQ SAPVYITIGD GGNLEGLATN
    410 420 430 440 450
    MTDPQPEYSA FREASFGHAT LDIKNRTHAY YSWHRNQDGY AVEADSMWVS
    460
    NRFWHPVDDS TTTKL
    Length:465
    Mass (Da):53,401
    Last modified:May 1, 2000 - v1
    Checksum:i49C0DCBA1CD8DF72
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200826 mRNA. Translation: AAF19822.1.
    PIRiT51095.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200826 mRNA. Translation: AAF19822.1.
    PIRiT51095.

    3D structure databases

    ProteinModelPortaliQ9SDZ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15154.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    PF16656. Pur_ac_phosph_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPPAF2_IPOBA
    AccessioniPrimary (citable) accession number: Q9SDZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 1, 2000
    Last modified: November 30, 2016
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.