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Q9SDZ9

- PPAF2_IPOBA

UniProt

Q9SDZ9 - PPAF2_IPOBA

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Protein
Purple acid phosphatase 2
Gene
PAP2
Organism
Ipomoea batatas (Sweet potato) (Convolvulus batatas)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Binds 1 iron ion per subunit By similarity.1 Publication
Binds 1 manganese ion per subunit. Can also use zinc, copper and magnesium ions.1 Publication

Absorptioni

Abs(max)=560 nm1 Publication

Kineticsi

  1. KM=95 µM for p-NPP (at pH 4.9 and 25 degrees Celsius)
  2. KM=120 µM for ATP (at pH 4.9 and 25 degrees Celsius)
  3. KM=180 µM for ADP (at pH 4.9 and 25 degrees Celsius)
  4. KM=360 µM for AMP (at pH 4.9 and 25 degrees Celsius)
  5. KM=75 µM for pyrophosphate (at pH 4.9 and 25 degrees Celsius)
  6. KM=490 µM for beta-glycerophosphate (at pH 4.9 and 25 degrees Celsius)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi164 – 1641Iron By similarity
Metal bindingi193 – 1931Iron By similarity
Metal bindingi193 – 1931Manganese By similarity
Metal bindingi196 – 1961Iron By similarity
Metal bindingi230 – 2301Manganese By similarity
Binding sitei230 – 2301Substrate By similarity
Metal bindingi315 – 3151Manganese By similarity
Active sitei325 – 3251Proton donor By similarity
Metal bindingi352 – 3521Manganese By similarity
Metal bindingi354 – 3541Iron By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15154.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 2 (EC:3.1.3.2)
Alternative name(s):
Manganese(II) purple acid phosphatase 2
Gene namesi
Name:PAP2
OrganismiIpomoea batatas (Sweet potato) (Convolvulus batatas)
Taxonomic identifieri4120 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed prediction
Add
BLAST
Chaini33 – 465433Purple acid phosphatase 2
PRO_5000057352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi138 – 1381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi172 – 1721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi303 – 3031N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi374 – 374Interchain By similarity
Glycosylationi400 – 4001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi425 – 4251N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9SDZ9.
SMRiQ9SDZ9. Positions 39-461.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni352 – 3543Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SDZ9-1 [UniParc]FASTAAdd to Basket

« Hide

MGASRTGCYL LAVVLAAVMN AAIAGITSSF IRKVEKTVDM PLDSDVFRVP    50
PGYNAPQQVH ITQGDHVGKA MIVSWVTVDE PGSSKVVYWS ENSQHKKVAR 100
GNIRTYTYFN YTSGYIHHCT IRNLEYNTKY YYEVGIGNTT RSFWFTTPPE 150
VGPDVPYTFG LIGDLGQSFD SNRTLTHYER NPIKGQAVLF VGDLSYADNY 200
PNHDNVRWDT WGRFVERSTA YQPWIWTAGN HEIDFAPEIG ETKPFKPFTK 250
RYHVPYKASG STETFWYPIK RASAYIIVLS SYSAYGKYTP QYKWLEEELP 300
KVNRTETPWL IVLMHSPWYN SYNYHYMEGE TMRVMYEPWF VQHKVDLVFA 350
GHVHAYERSE RVSNVAYDIV NGKCTPVRDQ SAPVYITIGD GGNLEGLATN 400
MTDPQPEYSA FREASFGHAT LDIKNRTHAY YSWHRNQDGY AVEADSMWVS 450
NRFWHPVDDS TTTKL 465
Length:465
Mass (Da):53,401
Last modified:May 1, 2000 - v1
Checksum:i49C0DCBA1CD8DF72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200826 mRNA. Translation: AAF19822.1.
PIRiT51095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200826 mRNA. Translation: AAF19822.1 .
PIRi T51095.

3D structure databases

ProteinModelPortali Q9SDZ9.
SMRi Q9SDZ9. Positions 39-461.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15154.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean."
    Schenk G., Ge Y., Carrington L.E., Wynne C.J., Searle I.R., Carroll B.J., Hamilton S., de Jersey J.
    Arch. Biochem. Biophys. 370:183-189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Golden.

Entry informationi

Entry nameiPPAF2_IPOBA
AccessioniPrimary (citable) accession number: Q9SDZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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