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Protein

NEDD8-conjugating enzyme Ubc12

Gene

RCE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like protein NEDD8/RUB1 from the ECR1-AXR1 E1 complex and catalyzes its covalent attachment to other proteins.2 Publications

Miscellaneous

Reduction in RCE1 levels leads to reduced organ length and defects in gravitropism.

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • NEDD8 transferase activity Source: TAIR

GO - Biological processi

  • protein neddylation Source: GO_Central
  • response to auxin Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-8951664 Neddylation
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme Ubc12 (EC:2.3.2.-)
Alternative name(s):
RUB1 carrier protein 1
RUB1-conjugating enzyme 1
Gene namesi
Name:RCE1
Ordered Locus Names:At4g36800
ORF Names:C7A10.560
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G36800
TAIRilocus:2115305 AT4G36800

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824941 – 184NEDD8-conjugating enzyme Ubc12Add BLAST184

Proteomic databases

PaxDbiQ9SDY5
PRIDEiQ9SDY5

PTM databases

iPTMnetiQ9SDY5

Expressioni

Tissue specificityi

Expressed in shoot, root and floral meristems, and in vascular tissues of leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9SDY5 baseline and differential
GenevisibleiQ9SDY5 AT

Interactioni

Subunit structurei

Interacts with RBX1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RBX1AQ940X72EBI-595116,EBI-532404

Protein-protein interaction databases

BioGridi15114, 5 interactors
IntActiQ9SDY5, 4 interactors
STRINGi3702.AT4G36800.1

Structurei

3D structure databases

ProteinModelPortaliQ9SDY5
SMRiQ9SDY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0420 Eukaryota
ENOG410XS81 LUCA
HOGENOMiHOG000233456
InParanoidiQ9SDY5
KOiK10579
OMAiMIKIWSM
OrthoDBiEOG09360LXI
PhylomeDBiQ9SDY5

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SDY5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGLFKVKEK QREQAQNATR GGASVKKQSA GELRLHKDIS ELNLPSSCSI
60 70 80 90 100
SFPNGKDDLM NFEVSIKPDD GYYHNGTFVF TFQVSPVYPH EAPKVKCKTK
110 120 130 140 150
VYHPNIDLEG NVCLNILRED WKPVLNINTV IYGLFHLFTE PNSEDPLNHD
160 170 180
AAAVLRDNPK LFETNVRRAM TGGYVGQTFF PRCI
Length:184
Mass (Da):20,787
Last modified:May 1, 2000 - v1
Checksum:i9F541991CED5C1E4
GO
Isoform 2 (identifier: Q9SDY5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-184: YHPNIDLEGN...VGQTFFPRCI → GLSSFYMPYI...FGRKRLPEHL

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:149
Mass (Da):17,007
Checksum:iF8E0DCAEDC3E9FB0
GO

Sequence cautioni

The sequence CAB16820 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80346 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034924102 – 184YHPNI…FPRCI → GLSSFYMPYIVYSFYFKIVC VVETLCWFSCLGLSSQYRFG RKRLPEHL in isoform 2. 1 PublicationAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202771 mRNA Translation: AAF19827.1
Z99708 Genomic DNA Translation: CAB16820.1 Sequence problems.
AL161590 Genomic DNA Translation: CAB80346.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86703.1
CP002687 Genomic DNA Translation: AEE86704.1
AY048210 mRNA Translation: AAK82473.1
AY097381 mRNA Translation: AAM19897.1
AK230295 mRNA Translation: BAF02096.1
PIRiE85434
RefSeqiNP_001154289.1, NM_001160817.1 [Q9SDY5-1]
NP_568008.4, NM_119844.6 [Q9SDY5-1]
UniGeneiAt.11882
At.24761
At.43436

Genome annotation databases

EnsemblPlantsiAT4G36800.1; AT4G36800.1; AT4G36800 [Q9SDY5-1]
AT4G36800.2; AT4G36800.2; AT4G36800 [Q9SDY5-1]
GeneIDi829833
GrameneiAT4G36800.1; AT4G36800.1; AT4G36800 [Q9SDY5-1]
AT4G36800.2; AT4G36800.2; AT4G36800 [Q9SDY5-1]
KEGGiath:AT4G36800

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRCE1_ARATH
AccessioniPrimary (citable) accession number: Q9SDY5
Secondary accession number(s): O23202, Q0WLB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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