Q9SD85 (F3PH_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 89.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Flavonoid 3'-monooxygenase EC=1.14.13.21 Alternative name(s): Cytochrome P450 75B1 Flavonoid 3'-hydroxylase Short name=AtF3'H Protein TRANSPARENT TESTA 7 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 513 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Convert naringenin to eriodictyol and dihydrokaempferol to dihydroquercetin. |
| Catalytic activity | A flavonoid + NADPH + O2 = a 3'-hydroxyflavonoid + NADP+ + H2O. |
| Cofactor | Heme group By similarity. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type III membrane protein Potential. |
| Tissue specificity | High expression in siliques and to a lower extent in stems, flowers and senescing leaves. |
| Induction | By UV light treatment. |
| Miscellaneous | May act as a membrane anchor for localization of other, soluble, flavonoid enzymes to the endoplasmic reticulum. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Flavonoid biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to auxin stimulusInferred from expression pattern. Source: TAIR |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3'-monooxygenase activityInferred from electronic annotation. Source: EC heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 513 | 513 | Flavonoid 3'-monooxygenase | PRO_0000052136 | |||||
Regions | |||||||||
| Transmembrane | 1 – 21 | 21 | Helical; Potential | ||||||
| Topological domain | 22 – 513 | 492 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Metal binding | 445 | 1 | Iron (heme axial ligand) By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme." Schoenbohm C., Martens S., Eder C., Forkmann G., Weisshaar B. Biol. Chem. 381:749-753(2000) [PubMed: 11030432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia and cv. Landsberg erecta. |
| [2] | "Isolation of a flavonoid 3'-hydroxylase gene corresponding to the Tt7 locus of Arabidopsis thaliana." Cordiner T.D., Barri-Rewell G., Brugliera F., Cobbett C., Holton T.A. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta. |
| [3] | "Sequence of flavonoid 3'hydroxylase (F3'H)." Saslowsky D., Winkel-Shirley B. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta. |
| [4] | "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F.Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [5] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [6] | "Localization of flavonoid enzymes in Arabidopsis roots." Saslowsky D., Winkel-Shirley B. Plant J. 27:37-48(2001) [PubMed: 11489181] [Abstract] Cited for: CHARACTERIZATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF271651 mRNA. Translation: AAG16746.1. AF271650 mRNA. Translation: AAG16745.1. AF155171 Genomic DNA. Translation: AAF73253.1. AF241646 AF241645 Genomic DNA. Translation: AAF60189.1.AL133421 Genomic DNA. Translation: CAB62611.1. CP002688 Genomic DNA. Translation: AED91232.1. |
| IPI | IPI00549090. |
| PIR | T45624. |
| RefSeq | NP_196416.1. NM_120881.2. |
| UniGene | At.12059. |
3D structure databases | |
| ProteinModelPortal | Q9SD85. |
| SMR | Q9SD85. Positions 28-502. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9SD85. |
Proteomic databases | |
| PRIDE | Q9SD85. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT5G07990.1; AT5G07990.1; AT5G07990. |
| GeneID | 830693. |
| GenomeReviews | Gene locus AT5G07990 in contig BA000015_GR. |
| KEGG | ath:AT5G07990. |
| NMPDR | fig|3702.1.peg.22940. |
Organism-specific databases | |
| GeneFarm | 1262. 94. |
| TAIR | At5g07990. |
Phylogenomic databases | |
| GeneTree | EPGT00070000027890. |
| HOGENOM | HBG749920. |
| InParanoid | Q9SD85. |
| OMA | HIASESC. |
| PhylomeDB | Q9SD85. |
| ProtClustDB | PLN02687. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.21. 399. |
Gene expression databases | |
| ArrayExpress | Q9SD85. |
| Genevestigator | Q9SD85. |
| GermOnline | AT5G07990. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| KO | K05280. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| SUPFAM | SSF48264. Cytochrome_P450. 1 hit. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | F3PH_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9SD85 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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