Q9SD85 (F3PH_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavonoid 3'-monooxygenase
EC=1.14.13.21

Alternative name(s):
Cytochrome P450 75B1
Flavonoid 3'-hydroxylase
Short name=AtF3'H
Protein TRANSPARENT TESTA 7

Gene names

Name:	CYP75B1
Synonyms:	F3'H, TT7
Ordered Locus Names:	At5g07990
ORF Names:	F13G24.190

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Convert naringenin to eriodictyol and dihydrokaempferol to dihydroquercetin.
Catalytic activity	A flavonoid + NADPH + O2 = a 3'-hydroxyflavonoid + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein Potential.
Tissue specificity	High expression in siliques and to a lower extent in stems, flowers and senescing leaves.
Induction	By UV light treatment.
Miscellaneous	May act as a membrane anchor for localization of other, soluble, flavonoid enzymes to the endoplasmic reticulum.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to auxin stimulus Inferred from expression pattern. Source: TAIR
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavonoid 3'-monooxygenase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Flavonoid 3'-monooxygenase		PRO_0000052136
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Topological domain	22 – 513	492	Cytoplasmic Potential
Sites
	Metal binding	445	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SD85 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C0C740FBE4559C40	FASTA	513	56,787
	10 20 30 40 50 60 MATLFLTILL ATVLFLILRI FSHRRNRSHN NRLPPGPNPW PIIGNLPHMG TKPHRTLSAM 70 80 90 100 110 120 VTTYGPILHL RLGFVDVVVA ASKSVAEQFL KIHDANFASR PPNSGAKHMA YNYQDLVFAP 130 140 150 160 170 180 YGHRWRLLRK ISSVHLFSAK ALEDFKHVRQ EEVGTLTREL VRVGTKPVNL GQLVNMCVVN 190 200 210 220 230 240 ALGREMIGRR LFGADADHKA DEFRSMVTEM MALAGVFNIG DFVPSLDWLD LQGVAGKMKR 250 260 270 280 290 300 LHKRFDAFLS SILKEHEMNG QDQKHTDMLS TLISLKGTDL DGDGGSLTDT EIKALLLNMF 310 320 330 340 350 360 TAGTDTSAST VDWAIAELIR HPDIMVKAQE ELDIVVGRDR PVNESDIAQL PYLQAVIKEN 370 380 390 400 410 420 FRLHPPTPLS LPHIASESCE INGYHIPKGS TLLTNIWAIA RDPDQWSDPL AFKPERFLPG 430 440 450 460 470 480 GEKSGVDVKG SDFELIPFGA GRRICAGLSL GLRTIQFLTA TLVQGFDWEL AGGVTPEKLN 490 500 510 MEESYGLTLQ RAVPLVVHPK PRLAPNVYGL GSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme." Schoenbohm C., Martens S., Eder C., Forkmann G., Weisshaar B. Biol. Chem. 381:749-753(2000) [PubMed: 11030432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia and cv. Landsberg erecta.
[2]	"Isolation of a flavonoid 3'-hydroxylase gene corresponding to the Tt7 locus of Arabidopsis thaliana." Cordiner T.D., Barri-Rewell G., Brugliera F., Cobbett C., Holton T.A. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[3]	"Sequence of flavonoid 3'hydroxylase (F3'H)." Saslowsky D., Winkel-Shirley B. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta.
[4]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Localization of flavonoid enzymes in Arabidopsis roots." Saslowsky D., Winkel-Shirley B. Plant J. 27:37-48(2001) [PubMed: 11489181] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF271651 mRNA. Translation: AAG16746.1. AF271650 mRNA. Translation: AAG16745.1. AF155171 Genomic DNA. Translation: AAF73253.1. AF241646 , AF241643, AF241644, AF241645 Genomic DNA. Translation: AAF60189.1. AL133421 Genomic DNA. Translation: CAB62611.1. CP002688 Genomic DNA. Translation: AED91232.1.
IPI	IPI00549090.
PIR	T45624.
RefSeq	NP_196416.1. NM_120881.2.
UniGene	At.12059.
3D structure databases
ProteinModelPortal	Q9SD85.
SMR	Q9SD85. Positions 28-502.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SD85.
Proteomic databases
PRIDE	Q9SD85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G07990.1; AT5G07990.1; AT5G07990.
GeneID	830693.
GenomeReviews	Gene locus AT5G07990 in contig BA000015_GR.
KEGG	ath:AT5G07990.
NMPDR	fig|3702.1.peg.22940.
Organism-specific databases
GeneFarm	1262. 94.
TAIR	At5g07990.
Phylogenomic databases
GeneTree	EPGT00070000027890.
HOGENOM	HBG749920.
InParanoid	Q9SD85.
OMA	HIASESC.
PhylomeDB	Q9SD85.
ProtClustDB	PLN02687.
Enzyme and pathway databases
BRENDA	1.14.13.21. 399.
Gene expression databases
ArrayExpress	Q9SD85.
Genevestigator	Q9SD85.
GermOnline	AT5G07990. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K05280.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F3PH_ARATH

Accession

Primary (citable) accession number: Q9SD85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program