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Protein

Alpha-glucan phosphorylase 2, cytosolic

Gene

PHS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity).By similarity

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

GO - Molecular functioni

GO - Biological processi

  • glycogen catabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G46970-MONOMER.
BRENDAi2.4.1.1. 399.
ReactomeiR-ATH-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucan phosphorylase 2, cytosolic (EC:2.4.1.1)
Short name:
AtPHS2
Alternative name(s):
Alpha-glucan phosphorylase, H isozyme
Starch phosphorylase H
Gene namesi
Name:PHS2
Ordered Locus Names:At3g46970
ORF Names:F13I12.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G46970.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 841841Alpha-glucan phosphorylase 2, cytosolicPRO_0000188541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei687 – 6871N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ9SD76.
PRIDEiQ9SD76.

PTM databases

iPTMnetiQ9SD76.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SD76. baseline and differential.
GenevisibleiQ9SD76. AT.

Interactioni

Protein-protein interaction databases

BioGridi9170. 1 interaction.
STRINGi3702.AT3G46970.1.

Structurei

Secondary structure

1
841
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi27 – 4014Combined sources
Helixi51 – 8030Combined sources
Beta strandi84 – 885Combined sources
Helixi98 – 1047Combined sources
Helixi108 – 11710Combined sources
Helixi122 – 1265Combined sources
Helixi138 – 15215Combined sources
Beta strandi157 – 1626Combined sources
Beta strandi170 – 1745Combined sources
Beta strandi177 – 1815Combined sources
Beta strandi194 – 20613Combined sources
Beta strandi208 – 2114Combined sources
Beta strandi217 – 23519Combined sources
Beta strandi242 – 25110Combined sources
Helixi254 – 2563Combined sources
Helixi259 – 2635Combined sources
Helixi267 – 2704Combined sources
Helixi272 – 2809Combined sources
Helixi291 – 31828Combined sources
Beta strandi323 – 3253Combined sources
Helixi328 – 3303Combined sources
Helixi331 – 3344Combined sources
Beta strandi335 – 3428Combined sources
Turni343 – 3464Combined sources
Helixi347 – 35610Combined sources
Turni357 – 3593Combined sources
Helixi363 – 37311Combined sources
Beta strandi374 – 3774Combined sources
Helixi383 – 3853Combined sources
Beta strandi388 – 3903Combined sources
Helixi391 – 3977Combined sources
Helixi399 – 41921Combined sources
Helixi421 – 4266Combined sources
Helixi427 – 4304Combined sources
Beta strandi431 – 4344Combined sources
Beta strandi437 – 4393Combined sources
Beta strandi441 – 4433Combined sources
Helixi444 – 4518Combined sources
Beta strandi453 – 4597Combined sources
Helixi460 – 4689Combined sources
Turni469 – 4713Combined sources
Helixi472 – 4776Combined sources
Beta strandi481 – 4844Combined sources
Helixi492 – 4976Combined sources
Helixi500 – 51011Combined sources
Helixi514 – 5163Combined sources
Helixi519 – 52810Combined sources
Helixi532 – 55726Combined sources
Beta strandi565 – 5728Combined sources
Helixi576 – 5783Combined sources
Helixi580 – 59617Combined sources
Helixi599 – 6024Combined sources
Beta strandi608 – 6136Combined sources
Helixi621 – 63818Combined sources
Turni641 – 6466Combined sources
Beta strandi647 – 6526Combined sources
Helixi657 – 66610Combined sources
Beta strandi668 – 6725Combined sources
Helixi683 – 6908Combined sources
Beta strandi694 – 6974Combined sources
Helixi702 – 7109Combined sources
Helixi712 – 7143Combined sources
Beta strandi715 – 7173Combined sources
Turni722 – 7243Combined sources
Helixi725 – 7339Combined sources
Helixi741 – 75111Combined sources
Turni752 – 7554Combined sources
Helixi761 – 7644Combined sources
Helixi765 – 7673Combined sources
Beta strandi769 – 7724Combined sources
Helixi781 – 80020Combined sources
Helixi802 – 81413Combined sources
Helixi817 – 8193Combined sources
Helixi821 – 83111Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BQEX-ray1.70A/B1-841[»]
4BQFX-ray2.35A/B1-841[»]
4BQIX-ray1.90A/B1-841[»]
ProteinModelPortaliQ9SD76.
SMRiQ9SD76. Positions 15-841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiQ9SD76.
KOiK00688.
OMAiSGEENNY.
OrthoDBiEOG093602M4.
PhylomeDBiQ9SD76.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SD76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANANGKAAT SLPEKISAKA NPEADDATEI AGNIVYHAKY SPHFSPLKFG
60 70 80 90 100
PEQALYATAE SLRDRLIQLW NETYVHFNKV DPKQTYYLSM EYLQGRALTN
110 120 130 140 150
AIGNLNLQGP YADALRTLGY ELEEIAEQEK DAALGNGGLG RLASCFLDSM
160 170 180 190 200
ATLNLPAWGY GLRYRHGLFK QIITKKGQEE IPEDWLEKFS PWEIVRHDVV
210 220 230 240 250
FPVRFFGKVQ VNPDGSRKWV DGDVVQALAY DVPIPGYGTK NTISLRLWEA
260 270 280 290 300
KARAEDLDLF QFNEGEYELA AQLHSRAQQI CTVLYPGDAT ENGKLLRLKQ
310 320 330 340 350
QFFLCSASLQ DIISRFHERS TTEGSRKWSE FPSKVAVQMN DTHPTLAIPE
360 370 380 390 400
LMRLLMDDNG LGWDEAWDVT SKTVAYTNHT VLPEALEKWS QSLMWKLLPR
410 420 430 440 450
HMEIIEEIDK RFVQTIRDTR VDLEDKISSL SILDNNPQKP VVRMANLCVV
460 470 480 490 500
SSHTVNGVAQ LHSDILKAEL FADYVSIWPN KFQNKTNGIT PRRWLRFCSP
510 520 530 540 550
ELSDIITKWL KTDKWITDLD LLTGLRQFAD NEELQSEWAS AKTANKKRLA
560 570 580 590 600
QYIERVTGVS IDPTSLFDIQ VKRIHEYKRQ LMNILGVVYR FKKLKEMKPE
610 620 630 640 650
ERKKTVPRTV MIGGKAFATY TNAKRIVKLV NDVGDVVNSD PEVNEYLKVV
660 670 680 690 700
FVPNYNVTVA EMLIPGSELS QHISTAGMEA SGTSNMKFAL NGCLIIGTLD
710 720 730 740 750
GANVEIREEV GEENFFLFGA TADQVPRLRK EREDGLFKPD PRFEEAKQFV
760 770 780 790 800
KSGVFGSYDY GPLLDSLEGN TGFGRGDYFL VGYDFPSYMD AQAKVDEAYK
810 820 830 840
DRKGWLKMSI LSTAGSGKFS SDRTIAQYAK EIWNIEACPV P
Length:841
Mass (Da):95,159
Last modified:May 1, 2000 - v1
Checksum:i4B50C195FBB3F7A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311A → P in AAL10498 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133292 Genomic DNA. Translation: CAB61943.1.
CP002686 Genomic DNA. Translation: AEE78225.1.
AY056807 mRNA. Translation: AAL10498.1.
AY090236 mRNA. Translation: AAL90900.1.
BT003012 mRNA. Translation: AAO23577.1.
PIRiT45633.
RefSeqiNP_190281.1. NM_114564.2.
UniGeneiAt.879.

Genome annotation databases

EnsemblPlantsiAT3G46970.1; AT3G46970.1; AT3G46970.
GeneIDi823850.
GrameneiAT3G46970.1; AT3G46970.1; AT3G46970.
KEGGiath:AT3G46970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133292 Genomic DNA. Translation: CAB61943.1.
CP002686 Genomic DNA. Translation: AEE78225.1.
AY056807 mRNA. Translation: AAL10498.1.
AY090236 mRNA. Translation: AAL90900.1.
BT003012 mRNA. Translation: AAO23577.1.
PIRiT45633.
RefSeqiNP_190281.1. NM_114564.2.
UniGeneiAt.879.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BQEX-ray1.70A/B1-841[»]
4BQFX-ray2.35A/B1-841[»]
4BQIX-ray1.90A/B1-841[»]
ProteinModelPortaliQ9SD76.
SMRiQ9SD76. Positions 15-841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9170. 1 interaction.
STRINGi3702.AT3G46970.1.

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

PTM databases

iPTMnetiQ9SD76.

Proteomic databases

PaxDbiQ9SD76.
PRIDEiQ9SD76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G46970.1; AT3G46970.1; AT3G46970.
GeneIDi823850.
GrameneiAT3G46970.1; AT3G46970.1; AT3G46970.
KEGGiath:AT3G46970.

Organism-specific databases

TAIRiAT3G46970.

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiQ9SD76.
KOiK00688.
OMAiSGEENNY.
OrthoDBiEOG093602M4.
PhylomeDBiQ9SD76.

Enzyme and pathway databases

BioCyciARA:AT3G46970-MONOMER.
BRENDAi2.4.1.1. 399.
ReactomeiR-ATH-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiQ9SD76.

Gene expression databases

ExpressionAtlasiQ9SD76. baseline and differential.
GenevisibleiQ9SD76. AT.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHS2_ARATH
AccessioniPrimary (citable) accession number: Q9SD76
Secondary accession number(s): Q93ZL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.