Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Receptor-like protein kinase FERONIA

Gene

FER

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor-like protein kinase that mediates the female control of male gamete delivery during fertilization, including growth cessation of compatible pollen tubes ensuring a reproductive isolation barriers, by regulating MLO7 subcellular polarization upon pollen tube perception in the female gametophyte synergids. Required for cell elongation during vegetative growth, mostly in a brassinosteroids- (BR-) independent manner. Acts as an upstream regulator for the Rac/Rop-signaling pathway that controls ROS-mediated root hair development. Seems to regulates a cross-talk between brassinosteroids and ethylene signaling pathways during hypocotyl elongation. Negative regulator of brassinosteroid response in light-grown hypocotyls, but required for brassinosteroid response in etiolated seedlings. Mediates sensitivity to powdery mildew (e.g. Golovinomyces orontii). Positive regulator of auxin-promoted growth that represses the abscisic acid (ABA) signaling via the activation of ABI2 phosphatase. Required for RALF1-mediated extracellular alkalinization in a signaling pathway preventing cell expansion.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei565ATPPROSITE-ProRule annotation1
Active sitei661Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi542 – 550ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • brassinosteroid mediated signaling pathway Source: UniProtKB-KW
  • defense response to fungus Source: TAIR
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • pollen tube reception Source: TAIR
  • post-embryonic development Source: TAIR
  • protein autophosphorylation Source: TAIR
  • response to brassinosteroid Source: UniProtKB
  • response to ethylene Source: UniProtKB
  • root development Source: UniProtKB
  • single fertilization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Abscisic acid signaling pathway, Brassinosteroid signaling pathway, Ethylene signaling pathway, Fertilization

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-like protein kinase FERONIA (EC:2.7.11.1)
Alternative name(s):
Protein SIRENE
Gene namesi
Name:FER
Synonyms:AAK1, SIR, SRN
Ordered Locus Names:At3g51550
ORF Names:F26O13.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G51550.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 447ExtracellularSequence analysisAdd BLAST420
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 895CytoplasmicSequence analysisAdd BLAST427

GO - Cellular componenti

  • filiform apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality due to impaired fertilization. The pollen tube fails to arrest and continues to grow inside the female gametophyte. Disturbed MLO7 relocalization to the filiform apparatus upon pollen tube arrival at the micropyle. Severe cell elongation defect in RNAi mutants with decreased FER expression. In fer-2 seedlings, altered responsiveness to brassinosteroids but increased ethylene response during the regulation of hypocotyl elongation. Increased resistance to powdery mildew (e.g. Golovinomyces orontii). Abscisic acid- (ABA)- hypersensitive response. Longer roots. Insensitivity to RALF1-mediated root-growth inbibition but hypersensitive to cation lithium inhibition.5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi565K → R: Loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000038655628 – 895Receptor-like protein kinase FERONIAAdd BLAST868

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi124N-linked (GlcNAc...)Sequence analysis1
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi219N-linked (GlcNAc...)Sequence analysis1
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Glycosylationi330N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1
Modified residuei858Phosphoserine1 Publication1
Modified residuei866PhosphoserineCombined sources1
Modified residuei871Phosphoserine1 Publication1
Modified residuei874Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylated.
Phosphorylated at Ser-858, Ser-871 and Ser-874 upon activation by RALF1.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9SCZ4.

PTM databases

iPTMnetiQ9SCZ4.
SwissPalmiQ9SCZ4.

Expressioni

Tissue specificityi

Expressed in leaves, buds, flowers, siliques, young ovules primordia, and young anthers with immature pollen, but not detected in mature pollen. Highest expression in the synergid cells of the female gametophyte.2 Publications

Developmental stagei

Detected in floral apices, young ovule primordia, and young anthers with immature pollen, but not in older anthers with mature pollen. After fertilization, expressed in globular embryos.1 Publication

Inductioni

By brassinosteroids (BR).1 Publication

Gene expression databases

GenevisibleiQ9SCZ4. AT.

Interactioni

Subunit structurei

Interacts with ROPGEF1 (PubMed:20876100). Interacts with RALF1; triggering phosphorylation status and subsequent activation (PubMed:24458638). Interacts with LRE and LLG1 (PubMed:26052747).3 Publications

Protein-protein interaction databases

BioGridi9636. 7 interactors.
DIPiDIP-59394N.
STRINGi3702.AT3G51550.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SCZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini536 – 810Protein kinasePROSITE-ProRule annotationAdd BLAST275

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000238317.
InParanoidiQ9SCZ4.
OMAiLTWMLQV.
OrthoDBiEOG093601SG.
PhylomeDBiQ9SCZ4.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR024788. Malectin-like_Carb-bd_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12819. Malectin_like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITEGRFRL SLLLLLLLIS AATLISAADY SPTEKILLNC GGGASNLTDT
60 70 80 90 100
DNRIWISDVK SKFLSSSSED SKTSPALTQD PSVPEVPYMT ARVFRSPFTY
110 120 130 140 150
TFPVASGRKF VRLYFYPNSY DGLNATNSLF SVSFGPYTLL KNFSASQTAE
160 170 180 190 200
ALTYAFIIKE FVVNVEGGTL NMTFTPESAP SNAYAFVNGI EVTSMPDMYS
210 220 230 240 250
STDGTLTMVG SSGSVTIDNS TALENVYRLN VGGNDISPSA DTGLYRSWYD
260 270 280 290 300
DQPYIFGAGL GIPETADPNM TIKYPTGTPT YVAPVDVYST ARSMGPTAQI
310 320 330 340 350
NLNYNLTWIF SIDSGFTYLV RLHFCEVSSN ITKINQRVFT IYLNNQTAEP
360 370 380 390 400
EADVIAWTSS NGVPFHKDYV VNPPEGNGQQ DLWLALHPNP VNKPEYYDSL
410 420 430 440 450
LNGVEIFKMN TSDGNLAGTN PIPGPQVTAD PSKVLRPTTR KSKSNTAIIA
460 470 480 490 500
GAASGAVVLA LIIGFCVFGA YRRRKRGDYQ PASDATSGWL PLSLYGNSHS
510 520 530 540 550
AGSAKTNTTG SYASSLPSNL CRHFSFAEIK AATKNFDESR VLGVGGFGKV
560 570 580 590 600
YRGEIDGGTT KVAIKRGNPM SEQGVHEFQT EIEMLSKLRH RHLVSLIGYC
610 620 630 640 650
EENCEMILVY DYMAHGTMRE HLYKTQNPSL PWKQRLEICI GAARGLHYLH
660 670 680 690 700
TGAKHTIIHR DVKTTNILLD EKWVAKVSDF GLSKTGPTLD HTHVSTVVKG
710 720 730 740 750
SFGYLDPEYF RRQQLTEKSD VYSFGVVLFE ALCARPALNP TLAKEQVSLA
760 770 780 790 800
EWAPYCYKKG MLDQIVDPYL KGKITPECFK KFAETAMKCV LDQGIERPSM
810 820 830 840 850
GDVLWNLEFA LQLQESAEEN GKGVCGDMDM DEIKYDDGNC KGKNDKSSDV
860 870 880 890
YEGNVTDSRS SGIDMSIGGR SLASEDSDGL TPSAVFSQIM NPKGR
Length:895
Mass (Da):98,150
Last modified:May 1, 2000 - v1
Checksum:iCFAEFE8E5146BC26
GO

Sequence cautioni

The sequence CAB92960 differs from that shown. Sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7R → Q in ABT18100 (PubMed:17673660).Curated1
Sequence conflicti17 – 18Missing in ABT18100 (PubMed:17673660).Curated2
Sequence conflicti179A → S in ABT18100 (PubMed:17673660).Curated1
Sequence conflicti546G → V in AAK59558 (PubMed:14593172).Curated1
Sequence conflicti761M → V in BAF01824 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF681137 Genomic DNA. Translation: ABT18100.1.
AL133452 Genomic DNA. Translation: CAB63019.1.
CP002686 Genomic DNA. Translation: AEE78805.1.
AY035053 mRNA. Translation: AAK59558.1.
AJ242671 mRNA. Translation: CAB92960.1. Sequence problems.
AK230000 mRNA. Translation: BAF01824.1.
PIRiT45786.
RefSeqiNP_190723.1. NM_115014.5.
UniGeneiAt.656.

Genome annotation databases

EnsemblPlantsiAT3G51550.1; AT3G51550.1; AT3G51550.
GeneIDi824318.
GrameneiAT3G51550.1; AT3G51550.1; AT3G51550.
KEGGiath:AT3G51550.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF681137 Genomic DNA. Translation: ABT18100.1.
AL133452 Genomic DNA. Translation: CAB63019.1.
CP002686 Genomic DNA. Translation: AEE78805.1.
AY035053 mRNA. Translation: AAK59558.1.
AJ242671 mRNA. Translation: CAB92960.1. Sequence problems.
AK230000 mRNA. Translation: BAF01824.1.
PIRiT45786.
RefSeqiNP_190723.1. NM_115014.5.
UniGeneiAt.656.

3D structure databases

ProteinModelPortaliQ9SCZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9636. 7 interactors.
DIPiDIP-59394N.
STRINGi3702.AT3G51550.1.

PTM databases

iPTMnetiQ9SCZ4.
SwissPalmiQ9SCZ4.

Proteomic databases

PaxDbiQ9SCZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G51550.1; AT3G51550.1; AT3G51550.
GeneIDi824318.
GrameneiAT3G51550.1; AT3G51550.1; AT3G51550.
KEGGiath:AT3G51550.

Organism-specific databases

TAIRiAT3G51550.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000238317.
InParanoidiQ9SCZ4.
OMAiLTWMLQV.
OrthoDBiEOG093601SG.
PhylomeDBiQ9SCZ4.

Miscellaneous databases

PROiQ9SCZ4.

Gene expression databases

GenevisibleiQ9SCZ4. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR024788. Malectin-like_Carb-bd_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12819. Malectin_like. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFERON_ARATH
AccessioniPrimary (citable) accession number: Q9SCZ4
Secondary accession number(s): A7U523
, Q0WM33, Q94C93, Q9M4G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Female paralog of ANXUR, a male factor expressed in pollen tube that controls release of the sperm cell.
Named after the Etruscan goddess of fertility Feronia.
'Sirene' means siren in French.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.