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Protein

Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplastic

Gene

FKBP13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Responsive of the major PPIase activity in the chloroplast thylakoid lumen. Regulates the accumulation of Rieske protein, an essential component of the photosynthetic electron transport chain.3 Publications

Miscellaneous

The interaction between FKBP and the Rieske protein probably occurs before they are imported into the thylakoid.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).Curated

Enzyme regulationi

PPIase activity is optimal in oxidized form (S-S) and minimal in reduced form (SH). Reduction of the oxidized form is mediated by thioredoxin (TRX-M).1 Publication

GO - Molecular functioni

  • FK506 binding Source: GO_Central
  • peptidyl-prolyl cis-trans isomerase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Rotamase

Enzyme and pathway databases

BRENDAi5.2.1.8 399

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplastic1 Publication (EC:5.2.1.8Curated)
Short name:
PPIase FKBP131 Publication
Alternative name(s):
FK506-binding protein 1
FK506-binding protein 131 Publication
Short name:
AtFKBP131 Publication
Immunophilin FKBP131 Publication
Rotamase
Gene namesi
Name:FKBP131 Publication
Synonyms:FKBP22-11 Publication, FKBPK
Ordered Locus Names:At5g45680Imported
ORF Names:MRA19.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G45680
TAIRilocus:2171958 AT5G45680

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84C → S: Reduced PPIase activity; when associated with S-96. 1 Publication1
Mutagenesisi96C → S: Reduced PPIase activity; when associated with S-84. 1 Publication1
Mutagenesisi185C → S: Reduced PPIase activity; when associated with S-190. 1 Publication1
Mutagenesisi190C → S: Reduced PPIase activity; when associated with S-185. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 79Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000002552680 – 208Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplasticAdd BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi84 ↔ 961 Publication
Disulfide bondi185 ↔ 1901 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9SCY2
PRIDEiQ9SCY2

Expressioni

Tissue specificityi

Expressed in stems, leaves and developing flower buds, but not in roots.

Gene expression databases

ExpressionAtlasiQ9SCY2 baseline and differential
GenevisibleiQ9SCY2 AT

Interactioni

Subunit structurei

Interacts in vitro with LTO1 (PubMed:25412899). The precursor, but not the mature form of the protein, interacts with the Rieske protein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP0AA252EBI-2895757,EBI-368542From Escherichia coli (strain K12).

Protein-protein interaction databases

BioGridi19856, 2 interactors
IntActiQ9SCY2, 1 interactor
STRINGi3702.AT5G45680.1

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi94 – 99Combined sources6
Beta strandi111 – 119Combined sources9
Beta strandi125 – 128Combined sources4
Helixi129 – 132Combined sources4
Beta strandi136 – 139Combined sources4
Beta strandi142 – 145Combined sources4
Helixi147 – 154Combined sources8
Beta strandi167 – 172Combined sources6
Helixi174 – 176Combined sources3
Turni177 – 181Combined sources5
Beta strandi183 – 186Combined sources4
Beta strandi189 – 192Combined sources4
Beta strandi198 – 207Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U79X-ray1.85A/B/C/D/E80-208[»]
1Y0OX-ray1.89A/B/C/D/E80-208[»]
ProteinModelPortaliQ9SCY2
SMRiQ9SCY2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SCY2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 208PPIase FKBP-typePROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0549 Eukaryota
COG0545 LUCA
HOGENOMiHOG000154887
InParanoidiQ9SCY2
KOiK01802
OMAiETTSCEF
OrthoDBiEOG09360OGI
PhylomeDBiQ9SCY2

Family and domain databases

InterProiView protein in InterPro
IPR023566 PPIase_FKBP
IPR001179 PPIase_FKBP_dom
PANTHERiPTHR10516 PTHR10516, 1 hit
PfamiView protein in Pfam
PF00254 FKBP_C, 1 hit
PROSITEiView protein in PROSITE
PS50059 FKBP_PPIASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLGFSVGT CSPPSEKRKC RFLVNNSLNK AEAINLRNKQ KVSSDPELSF
60 70 80 90 100
AQLSSCGRRE AIIGFGFSIG LLDNVSALAE TTSCEFSVSP SGLAFCDKVV
110 120 130 140 150
GYGPEAVKGQ LIKAHYVGKL ENGKVFDSSY NRGKPLTFRI GVGEVIKGWD
160 170 180 190 200
QGILGSDGIP PMLTGGKRTL RIPPELAYGD RGAGCKGGSC LIPPASVLLF

DIEYIGKA
Length:208
Mass (Da):22,039
Last modified:March 1, 2001 - v2
Checksum:i4E6640FE7955A48F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → T in CAD35362 (PubMed:12424338).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ490171 mRNA Translation: CAD35362.1
AB012245 Genomic DNA Translation: BAB09210.1
CP002688 Genomic DNA Translation: AED95283.1
AY065047 mRNA Translation: AAL57682.1
AY091680 mRNA Translation: AAM10279.1
AJ242483 mRNA Translation: CAB64723.1
RefSeqiNP_199380.1, NM_123935.5
UniGeneiAt.28277

Genome annotation databases

EnsemblPlantsiAT5G45680.1; AT5G45680.1; AT5G45680
GeneIDi834607
GrameneiAT5G45680.1; AT5G45680.1; AT5G45680
KEGGiath:AT5G45680

Similar proteinsi

Entry informationi

Entry nameiFKB13_ARATH
AccessioniPrimary (citable) accession number: Q9SCY2
Secondary accession number(s): Q8M8T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health