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Protein

Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplastic

Gene

FKBP13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Responsive of the major PPIase activity in the chloroplast thylakoid lumen. Regulates the accumulation of Rieske protein, an essential component of the photosynthetic electron transport chain.3 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).Curated

Enzyme regulationi

PPIase activity is optimal in oxidized form (S-S) and minimal in reduced form (SH). Reduction of the oxidized form is mediated by thioredoxin (TRX-M).1 Publication

GO - Molecular functioni

  • FK506 binding Source: GO_Central
  • peptidyl-prolyl cis-trans isomerase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Enzyme and pathway databases

BRENDAi5.2.1.8. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplastic1 Publication (EC:5.2.1.8Curated)
Short name:
PPIase FKBP131 Publication
Alternative name(s):
FK506-binding protein 1
FK506-binding protein 131 Publication
Short name:
AtFKBP131 Publication
Immunophilin FKBP131 Publication
Rotamase
Gene namesi
Name:FKBP131 Publication
Synonyms:FKBP22-11 Publication, FKBPK
Ordered Locus Names:At5g45680Imported
ORF Names:MRA19.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45680.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid lumen Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: GO_Central
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84C → S: Reduced PPIase activity; when associated with S-96. 1 Publication1
Mutagenesisi96C → S: Reduced PPIase activity; when associated with S-84. 1 Publication1
Mutagenesisi185C → S: Reduced PPIase activity; when associated with S-190. 1 Publication1
Mutagenesisi190C → S: Reduced PPIase activity; when associated with S-185. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 79Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000002552680 – 208Peptidyl-prolyl cis-trans isomerase FKBP13, chloroplasticAdd BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi84 ↔ 961 Publication
Disulfide bondi185 ↔ 1901 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9SCY2.
PRIDEiQ9SCY2.

Expressioni

Tissue specificityi

Expressed in stems, leaves and developing flower buds, but not in roots.

Gene expression databases

GenevisibleiQ9SCY2. AT.

Interactioni

Subunit structurei

Interacts in vitro with LTO1 (PubMed:25412899). The precursor, but not the mature form of the protein, interacts with the Rieske protein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
trxAP0AA252EBI-2895757,EBI-368542From a different organism.

Protein-protein interaction databases

BioGridi19856. 2 interactors.
IntActiQ9SCY2. 1 interactor.
STRINGi3702.AT5G45680.1.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi94 – 99Combined sources6
Beta strandi111 – 119Combined sources9
Beta strandi125 – 128Combined sources4
Helixi129 – 132Combined sources4
Beta strandi136 – 139Combined sources4
Beta strandi142 – 145Combined sources4
Helixi147 – 154Combined sources8
Beta strandi167 – 172Combined sources6
Helixi174 – 176Combined sources3
Turni177 – 181Combined sources5
Beta strandi183 – 186Combined sources4
Beta strandi189 – 192Combined sources4
Beta strandi198 – 207Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U79X-ray1.85A/B/C/D/E80-208[»]
1Y0OX-ray1.89A/B/C/D/E80-208[»]
ProteinModelPortaliQ9SCY2.
SMRiQ9SCY2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9SCY2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 208PPIase FKBP-typePROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated
Contains 1 PPIase FKBP-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0549. Eukaryota.
COG0545. LUCA.
HOGENOMiHOG000154887.
InParanoidiQ9SCY2.
KOiK01802.
OMAiCGRREAI.
OrthoDBiEOG09360OGI.
PhylomeDBiQ9SCY2.

Family and domain databases

InterProiIPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
[Graphical view]
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLGFSVGT CSPPSEKRKC RFLVNNSLNK AEAINLRNKQ KVSSDPELSF
60 70 80 90 100
AQLSSCGRRE AIIGFGFSIG LLDNVSALAE TTSCEFSVSP SGLAFCDKVV
110 120 130 140 150
GYGPEAVKGQ LIKAHYVGKL ENGKVFDSSY NRGKPLTFRI GVGEVIKGWD
160 170 180 190 200
QGILGSDGIP PMLTGGKRTL RIPPELAYGD RGAGCKGGSC LIPPASVLLF

DIEYIGKA
Length:208
Mass (Da):22,039
Last modified:March 1, 2001 - v2
Checksum:i4E6640FE7955A48F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → T in CAD35362 (PubMed:12424338).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ490171 mRNA. Translation: CAD35362.1.
AB012245 Genomic DNA. Translation: BAB09210.1.
CP002688 Genomic DNA. Translation: AED95283.1.
AY065047 mRNA. Translation: AAL57682.1.
AY091680 mRNA. Translation: AAM10279.1.
AJ242483 mRNA. Translation: CAB64723.1.
RefSeqiNP_199380.1. NM_123935.5.
UniGeneiAt.28277.

Genome annotation databases

EnsemblPlantsiAT5G45680.1; AT5G45680.1; AT5G45680.
GeneIDi834607.
GrameneiAT5G45680.1; AT5G45680.1; AT5G45680.
KEGGiath:AT5G45680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ490171 mRNA. Translation: CAD35362.1.
AB012245 Genomic DNA. Translation: BAB09210.1.
CP002688 Genomic DNA. Translation: AED95283.1.
AY065047 mRNA. Translation: AAL57682.1.
AY091680 mRNA. Translation: AAM10279.1.
AJ242483 mRNA. Translation: CAB64723.1.
RefSeqiNP_199380.1. NM_123935.5.
UniGeneiAt.28277.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U79X-ray1.85A/B/C/D/E80-208[»]
1Y0OX-ray1.89A/B/C/D/E80-208[»]
ProteinModelPortaliQ9SCY2.
SMRiQ9SCY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19856. 2 interactors.
IntActiQ9SCY2. 1 interactor.
STRINGi3702.AT5G45680.1.

Proteomic databases

PaxDbiQ9SCY2.
PRIDEiQ9SCY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45680.1; AT5G45680.1; AT5G45680.
GeneIDi834607.
GrameneiAT5G45680.1; AT5G45680.1; AT5G45680.
KEGGiath:AT5G45680.

Organism-specific databases

TAIRiAT5G45680.

Phylogenomic databases

eggNOGiKOG0549. Eukaryota.
COG0545. LUCA.
HOGENOMiHOG000154887.
InParanoidiQ9SCY2.
KOiK01802.
OMAiCGRREAI.
OrthoDBiEOG09360OGI.
PhylomeDBiQ9SCY2.

Enzyme and pathway databases

BRENDAi5.2.1.8. 399.

Miscellaneous databases

EvolutionaryTraceiQ9SCY2.
PROiQ9SCY2.

Gene expression databases

GenevisibleiQ9SCY2. AT.

Family and domain databases

InterProiIPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
[Graphical view]
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFKB13_ARATH
AccessioniPrimary (citable) accession number: Q9SCY2
Secondary accession number(s): Q8M8T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The interaction between FKBP and the Rieske protein probably occurs before they are imported into the thylakoid.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.