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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic

Gene

DHAPRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in glycerolipid metabolism.By similarity

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Kineticsi

  1. KM=12.8 µM for glycerone phosphate1 Publication
  1. Vmax=3.2 µmol/sec/mg enzyme toward glycerone phosphate1 Publication

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92NADBy similarity1
Binding sitei149NADBy similarity1
Binding sitei172NAD; via amide nitrogenBy similarity1
Binding sitei172SubstrateBy similarity1
Binding sitei205NAD; via amide nitrogenBy similarity1
Active sitei257Proton acceptorBy similarity1
Binding sitei321NADBy similarity1
Binding sitei350NAD; via amide nitrogenBy similarity1
Binding sitei352NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 66NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic (EC:1.1.1.8)
Gene namesi
Name:DHAPRD
Ordered Locus Names:At5g40610
ORF Names:MNF13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40610.

Subcellular locationi

  • Plastidchloroplast 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32ChloroplastSequence analysisAdd BLAST32
ChainiPRO_000028787133 – 400Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplasticAdd BLAST368

Proteomic databases

PaxDbiQ9SCX9.

Expressioni

Tissue specificityi

Expressed in young seedlings, flowers and siliques. Expressed at low levels in roots.1 Publication

Gene expression databases

GenevisibleiQ9SCX9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G40610.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SCX9.
SMRiQ9SCX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni321 – 322Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
HOGENOMiHOG000246855.
InParanoidiQ9SCX9.
KOiK00006.
OMAiNFRSAKH.
OrthoDBiEOG09360DM6.
PhylomeDBiQ9SCX9.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN
60 70 80 90 100
DDSKSKVTVV GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE
110 120 130 140 150
KLNDVINKTN ENVKYLPGIK LGRNVVADPD LENAVKDANM LVFVTPHQFM
160 170 180 190 200
DGICKKLDGK ITGDVEAISL VKGMEVKKEG PCMISSLISK QLGINCCVLM
210 220 230 240 250
GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM VTPVHDVEGV
260 270 280 290 300
ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK
310 320 330 340 350
DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK
360 370 380 390 400
LQGVSTAREV YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL
Length:400
Mass (Da):43,791
Last modified:May 1, 2000 - v1
Checksum:i9A37BC876E8663CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242602 mRNA. Translation: CAB64726.1.
AB009052 Genomic DNA. Translation: BAB08532.1.
CP002688 Genomic DNA. Translation: AED94573.1.
BT000967 mRNA. Translation: AAN41367.1.
AY080863 mRNA. Translation: AAL87336.1.
RefSeqiNP_198877.1. NM_123425.5.
UniGeneiAt.695.

Genome annotation databases

EnsemblPlantsiAT5G40610.1; AT5G40610.1; AT5G40610.
GeneIDi834060.
GrameneiAT5G40610.1; AT5G40610.1; AT5G40610.
KEGGiath:AT5G40610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242602 mRNA. Translation: CAB64726.1.
AB009052 Genomic DNA. Translation: BAB08532.1.
CP002688 Genomic DNA. Translation: AED94573.1.
BT000967 mRNA. Translation: AAN41367.1.
AY080863 mRNA. Translation: AAL87336.1.
RefSeqiNP_198877.1. NM_123425.5.
UniGeneiAt.695.

3D structure databases

ProteinModelPortaliQ9SCX9.
SMRiQ9SCX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G40610.1.

Proteomic databases

PaxDbiQ9SCX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40610.1; AT5G40610.1; AT5G40610.
GeneIDi834060.
GrameneiAT5G40610.1; AT5G40610.1; AT5G40610.
KEGGiath:AT5G40610.

Organism-specific databases

TAIRiAT5G40610.

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
HOGENOMiHOG000246855.
InParanoidiQ9SCX9.
KOiK00006.
OMAiNFRSAKH.
OrthoDBiEOG09360DM6.
PhylomeDBiQ9SCX9.

Enzyme and pathway databases

UniPathwayiUPA00940.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ9SCX9.

Gene expression databases

GenevisibleiQ9SCX9. AT.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDA1_ARATH
AccessioniPrimary (citable) accession number: Q9SCX9
Secondary accession number(s): Q8RXI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.