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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic

Gene

DHAPRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in glycerolipid metabolism.By similarity

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Kineticsi

  1. KM=12.8 µM for glycerone phosphate1 Publication
  1. Vmax=3.2 µmol/sec/mg enzyme toward glycerone phosphate1 Publication

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92NADBy similarity1
Binding sitei149NADBy similarity1
Binding sitei172NAD; via amide nitrogenBy similarity1
Binding sitei172SubstrateBy similarity1
Binding sitei205NAD; via amide nitrogenBy similarity1
Active sitei257Proton acceptorBy similarity1
Binding sitei321NADBy similarity1
Binding sitei350NAD; via amide nitrogenBy similarity1
Binding sitei352NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 66NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-ATH-1483166 Synthesis of PA
UniPathwayiUPA00940

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic (EC:1.1.1.8)
Gene namesi
Name:DHAPRD
Ordered Locus Names:At5g40610
ORF Names:MNF13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40610
TAIRilocus:2168758 AT5G40610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32ChloroplastSequence analysisAdd BLAST32
ChainiPRO_000028787133 – 400Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplasticAdd BLAST368

Proteomic databases

PaxDbiQ9SCX9

Expressioni

Tissue specificityi

Expressed in young seedlings, flowers and siliques. Expressed at low levels in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ9SCX9 baseline and differential
GenevisibleiQ9SCX9 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G40610.1

Structurei

3D structure databases

ProteinModelPortaliQ9SCX9
SMRiQ9SCX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni321 – 322Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2711 Eukaryota
COG0240 LUCA
HOGENOMiHOG000246855
InParanoidiQ9SCX9
KOiK00006
OMAiPHQFIHK
OrthoDBiEOG09360DM6
PhylomeDBiQ9SCX9

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006168 G3P_DH_NAD-dep
IPR006109 G3P_DH_NAD-dep_C
IPR017751 G3P_DH_NAD-dep_euk
IPR011128 G3P_DH_NAD-dep_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF07479 NAD_Gly3P_dh_C, 1 hit
PF01210 NAD_Gly3P_dh_N, 1 hit
PIRSFiPIRSF000114 Glycerol-3-P_dh, 1 hit
PRINTSiPR00077 GPDHDRGNASE
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR03376 glycerol3P_DH, 1 hit
PROSITEiView protein in PROSITE
PS00957 NAD_G3PDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN
60 70 80 90 100
DDSKSKVTVV GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE
110 120 130 140 150
KLNDVINKTN ENVKYLPGIK LGRNVVADPD LENAVKDANM LVFVTPHQFM
160 170 180 190 200
DGICKKLDGK ITGDVEAISL VKGMEVKKEG PCMISSLISK QLGINCCVLM
210 220 230 240 250
GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM VTPVHDVEGV
260 270 280 290 300
ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK
310 320 330 340 350
DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK
360 370 380 390 400
LQGVSTAREV YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL
Length:400
Mass (Da):43,791
Last modified:May 1, 2000 - v1
Checksum:i9A37BC876E8663CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242602 mRNA Translation: CAB64726.1
AB009052 Genomic DNA Translation: BAB08532.1
CP002688 Genomic DNA Translation: AED94573.1
BT000967 mRNA Translation: AAN41367.1
AY080863 mRNA Translation: AAL87336.1
RefSeqiNP_198877.1, NM_123425.5
UniGeneiAt.695

Genome annotation databases

EnsemblPlantsiAT5G40610.1; AT5G40610.1; AT5G40610
GeneIDi834060
GrameneiAT5G40610.1; AT5G40610.1; AT5G40610
KEGGiath:AT5G40610

Similar proteinsi

Entry informationi

Entry nameiGPDA1_ARATH
AccessioniPrimary (citable) accession number: Q9SCX9
Secondary accession number(s): Q8RXI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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