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Q9SCX9

- GPDA1_ARATH

UniProt

Q9SCX9 - GPDA1_ARATH

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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic

Gene

DHAPRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in glycerolipid metabolism.By similarity

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Kineticsi

  1. KM=12.8 µM for glycerone phosphate1 Publication

Vmax=3.2 µmol/sec/mg enzyme toward glycerone phosphate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921NADBy similarity
Binding sitei149 – 1491NADBy similarity
Binding sitei172 – 1721NAD; via amide nitrogenBy similarity
Binding sitei172 – 1721SubstrateBy similarity
Binding sitei205 – 2051NAD; via amide nitrogenBy similarity
Active sitei257 – 2571Proton acceptorBy similarity
Binding sitei321 – 3211NADBy similarity
Binding sitei350 – 3501NAD; via amide nitrogenBy similarity
Binding sitei352 – 3521NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 666NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
  3. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  4. phospholipid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_185985. Synthesis of PA.
REACT_250956. Triglyceride Biosynthesis.
UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic (EC:1.1.1.8)
Gene namesi
Name:DHAPRD
Ordered Locus Names:At5g40610
ORF Names:MNF13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G40610.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232ChloroplastSequence AnalysisAdd
BLAST
Chaini33 – 400368Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplasticPRO_0000287871Add
BLAST

Proteomic databases

PaxDbiQ9SCX9.
PRIDEiQ9SCX9.

Expressioni

Tissue specificityi

Expressed in young seedlings, flowers and siliques. Expressed at low levels in roots.1 Publication

Gene expression databases

GenevestigatoriQ9SCX9.

Structurei

3D structure databases

ProteinModelPortaliQ9SCX9.
SMRiQ9SCX9. Positions 56-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni321 – 3222Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0240.
HOGENOMiHOG000246855.
InParanoidiQ9SCX9.
KOiK00006.
OMAiKIFCKGQ.
PhylomeDBiQ9SCX9.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCX9-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN
60 70 80 90 100
DDSKSKVTVV GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE
110 120 130 140 150
KLNDVINKTN ENVKYLPGIK LGRNVVADPD LENAVKDANM LVFVTPHQFM
160 170 180 190 200
DGICKKLDGK ITGDVEAISL VKGMEVKKEG PCMISSLISK QLGINCCVLM
210 220 230 240 250
GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM VTPVHDVEGV
260 270 280 290 300
ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK
310 320 330 340 350
DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK
360 370 380 390 400
LQGVSTAREV YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL
Length:400
Mass (Da):43,791
Last modified:May 1, 2000 - v1
Checksum:i9A37BC876E8663CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242602 mRNA. Translation: CAB64726.1.
AB009052 Genomic DNA. Translation: BAB08532.1.
CP002688 Genomic DNA. Translation: AED94573.1.
BT000967 mRNA. Translation: AAN41367.1.
AY080863 mRNA. Translation: AAL87336.1.
RefSeqiNP_198877.1. NM_123425.4.
UniGeneiAt.695.

Genome annotation databases

EnsemblPlantsiAT5G40610.1; AT5G40610.1; AT5G40610.
GeneIDi834060.
KEGGiath:AT5G40610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242602 mRNA. Translation: CAB64726.1 .
AB009052 Genomic DNA. Translation: BAB08532.1 .
CP002688 Genomic DNA. Translation: AED94573.1 .
BT000967 mRNA. Translation: AAN41367.1 .
AY080863 mRNA. Translation: AAL87336.1 .
RefSeqi NP_198877.1. NM_123425.4.
UniGenei At.695.

3D structure databases

ProteinModelPortali Q9SCX9.
SMRi Q9SCX9. Positions 56-383.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9SCX9.
PRIDEi Q9SCX9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G40610.1 ; AT5G40610.1 ; AT5G40610 .
GeneIDi 834060.
KEGGi ath:AT5G40610.

Organism-specific databases

TAIRi AT5G40610.

Phylogenomic databases

eggNOGi COG0240.
HOGENOMi HOG000246855.
InParanoidi Q9SCX9.
KOi K00006.
OMAi KIFCKGQ.
PhylomeDBi Q9SCX9.

Enzyme and pathway databases

UniPathwayi UPA00940 .
Reactomei REACT_185985. Synthesis of PA.
REACT_250956. Triglyceride Biosynthesis.

Gene expression databases

Genevestigatori Q9SCX9.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and biochemical characterizations of a plastidic glycerol-3-phosphate dehydrogenase from Arabidopsis."
    Wei Y., Periappuram C., Datla R., Selvaraj G., Zou J.
    Plant Physiol. Biochem. 39:841-848(2001)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGPDA1_ARATH
AccessioniPrimary (citable) accession number: Q9SCX9
Secondary accession number(s): Q8RXI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3