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Q9SCX9

- GPDA1_ARATH

UniProt

Q9SCX9 - GPDA1_ARATH

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic

Gene

DHAPRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in glycerolipid metabolism.By similarity

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Kineticsi

    1. KM=12.8 µM for glycerone phosphate1 Publication

    Vmax=3.2 µmol/sec/mg enzyme toward glycerone phosphate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921NADBy similarity
    Binding sitei149 – 1491NADBy similarity
    Binding sitei172 – 1721NAD; via amide nitrogenBy similarity
    Binding sitei172 – 1721SubstrateBy similarity
    Binding sitei205 – 2051NAD; via amide nitrogenBy similarity
    Active sitei257 – 2571Proton acceptorBy similarity
    Binding sitei321 – 3211NADBy similarity
    Binding sitei350 – 3501NAD; via amide nitrogenBy similarity
    Binding sitei352 – 3521NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi61 – 666NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycerol-3-phosphate catabolic process Source: InterPro
    3. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
    4. phospholipid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_185985. Synthesis of PA.
    UniPathwayiUPA00940.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic (EC:1.1.1.8)
    Gene namesi
    Name:DHAPRD
    Ordered Locus Names:At5g40610
    ORF Names:MNF13.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G40610.

    Subcellular locationi

    Plastidchloroplast 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232ChloroplastSequence AnalysisAdd
    BLAST
    Chaini33 – 400368Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplasticPRO_0000287871Add
    BLAST

    Proteomic databases

    PaxDbiQ9SCX9.
    PRIDEiQ9SCX9.

    Expressioni

    Tissue specificityi

    Expressed in young seedlings, flowers and siliques. Expressed at low levels in roots.1 Publication

    Gene expression databases

    GenevestigatoriQ9SCX9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SCX9.
    SMRiQ9SCX9. Positions 56-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni321 – 3222Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0240.
    HOGENOMiHOG000246855.
    InParanoidiQ9SCX9.
    KOiK00006.
    OMAiKIFCKGQ.
    PhylomeDBiQ9SCX9.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SCX9-1 [UniParc]FASTAAdd to Basket

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    MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN    50
    DDSKSKVTVV GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE 100
    KLNDVINKTN ENVKYLPGIK LGRNVVADPD LENAVKDANM LVFVTPHQFM 150
    DGICKKLDGK ITGDVEAISL VKGMEVKKEG PCMISSLISK QLGINCCVLM 200
    GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM VTPVHDVEGV 250
    ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK 300
    DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK 350
    LQGVSTAREV YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL 400
    Length:400
    Mass (Da):43,791
    Last modified:May 1, 2000 - v1
    Checksum:i9A37BC876E8663CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242602 mRNA. Translation: CAB64726.1.
    AB009052 Genomic DNA. Translation: BAB08532.1.
    CP002688 Genomic DNA. Translation: AED94573.1.
    BT000967 mRNA. Translation: AAN41367.1.
    AY080863 mRNA. Translation: AAL87336.1.
    RefSeqiNP_198877.1. NM_123425.4.
    UniGeneiAt.695.

    Genome annotation databases

    EnsemblPlantsiAT5G40610.1; AT5G40610.1; AT5G40610.
    GeneIDi834060.
    KEGGiath:AT5G40610.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ242602 mRNA. Translation: CAB64726.1 .
    AB009052 Genomic DNA. Translation: BAB08532.1 .
    CP002688 Genomic DNA. Translation: AED94573.1 .
    BT000967 mRNA. Translation: AAN41367.1 .
    AY080863 mRNA. Translation: AAL87336.1 .
    RefSeqi NP_198877.1. NM_123425.4.
    UniGenei At.695.

    3D structure databases

    ProteinModelPortali Q9SCX9.
    SMRi Q9SCX9. Positions 56-383.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9SCX9.
    PRIDEi Q9SCX9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G40610.1 ; AT5G40610.1 ; AT5G40610 .
    GeneIDi 834060.
    KEGGi ath:AT5G40610.

    Organism-specific databases

    TAIRi AT5G40610.

    Phylogenomic databases

    eggNOGi COG0240.
    HOGENOMi HOG000246855.
    InParanoidi Q9SCX9.
    KOi K00006.
    OMAi KIFCKGQ.
    PhylomeDBi Q9SCX9.

    Enzyme and pathway databases

    UniPathwayi UPA00940 .
    Reactomei REACT_185985. Synthesis of PA.

    Gene expression databases

    Genevestigatori Q9SCX9.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and biochemical characterizations of a plastidic glycerol-3-phosphate dehydrogenase from Arabidopsis."
      Wei Y., Periappuram C., Datla R., Selvaraj G., Zou J.
      Plant Physiol. Biochem. 39:841-848(2001)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiGPDA1_ARATH
    AccessioniPrimary (citable) accession number: Q9SCX9
    Secondary accession number(s): Q8RXI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3