Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Purple acid phosphatase 17

Gene

PAP17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metallo-phosphoesterase involved in phosphate metabolism. Has a peroxidase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.
2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphate and molybdate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531IronBy similarity
Metal bindingi86 – 861IronBy similarity
Metal bindingi86 – 861ZincBy similarity
Metal bindingi89 – 891IronBy similarity
Metal bindingi124 – 1241ZincBy similarity
Metal bindingi218 – 2181ZincBy similarity
Active sitei227 – 2271Proton donorBy similarity
Metal bindingi253 – 2531ZincBy similarity
Metal bindingi255 – 2551IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW
  3. peroxidase activity Source: UniProtKB-EC
  4. phosphatase activity Source: TAIR

GO - Biological processi

  1. cellular phosphate ion homeostasis Source: TAIR
  2. dephosphorylation Source: GOC
  3. response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G17790-MONOMER.
ReactomeiREACT_327526. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 17 (EC:3.1.3.2)
Alternative name(s):
Acid phosphatase type 5
Peroxidase (EC:1.11.1.7)
Gene namesi
Name:PAP17
Synonyms:ACP5
Ordered Locus Names:At3g17790
ORF Names:MEB5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17790.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cell surface Source: TAIR
  2. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 338307Purple acid phosphatase 17PRO_5000065319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCX8.
PRIDEiQ9SCX8.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

By phosphate starvation, during senescence, by ABA, by H2O2, and by salt stress.1 Publication

Gene expression databases

GenevestigatoriQ9SCX8.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9SCX8.
SMRiQ9SCX8. Positions 46-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 2553Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
InParanoidiQ9SCX8.
OMAiVHAEVTN.
PhylomeDBiQ9SCX8.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGRRSLMS ATASLSLLLC IFTTFVVVSN GELQRFIEPA KSDGSVSFIV
60 70 80 90 100
IGDWGRRGSF NQSLVAYQMG KIGEKIDLDF VVSTGDNFYD NGLFSEHDPN
110 120 130 140 150
FEQSFSNIYT APSLQKQWYS VLGNHDYRGD AEAQLSSVLR EIDSRWICLR
160 170 180 190 200
SFVVDAELVE MFFVDTTPFV KEYYTEADGH SYDWRAVPSR NSYVKALLRD
210 220 230 240 250
LEVSLKSSKA RWKIVVGHHA MRSIGHHGDT KELNEELLPI LKENGVDLYM
260 270 280 290 300
NGHDHCLQHM SDEDSPIQFL TSGAGSKAWR GDINPVTINP KLLKFYYDGQ
310 320 330
GFMSARFTHS DAEIVFYDVF GEILHKWVTS KQLLHSSV
Length:338
Mass (Da):38,297
Last modified:April 30, 2000 - v1
Checksum:i5183AE005E859740
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91M → I in AAM61192 (Ref. 7) Curated
Sequence conflicti115 – 1151Q → R in AAM61192 (Ref. 7) Curated
Sequence conflicti245 – 2451G → S in AAM61192 (Ref. 7) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133747 mRNA. Translation: CAC09923.1.
AJ243527 Genomic DNA. Translation: CAB63938.1.
AY818189 mRNA. Translation: AAV69751.1.
AB019230 Genomic DNA. Translation: BAB02702.1.
CP002686 Genomic DNA. Translation: AEE76007.1.
BT003135 mRNA. Translation: AAO24567.1.
AK228106 mRNA. Translation: BAF00065.1.
AY084629 mRNA. Translation: AAM61192.1.
RefSeqiNP_566587.1. NM_112660.5.
UniGeneiAt.22111.
At.71032.
At.74878.
At.74926.

Genome annotation databases

EnsemblPlantsiAT3G17790.1; AT3G17790.1; AT3G17790.
GeneIDi821047.
KEGGiath:AT3G17790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133747 mRNA. Translation: CAC09923.1.
AJ243527 Genomic DNA. Translation: CAB63938.1.
AY818189 mRNA. Translation: AAV69751.1.
AB019230 Genomic DNA. Translation: BAB02702.1.
CP002686 Genomic DNA. Translation: AEE76007.1.
BT003135 mRNA. Translation: AAO24567.1.
AK228106 mRNA. Translation: BAF00065.1.
AY084629 mRNA. Translation: AAM61192.1.
RefSeqiNP_566587.1. NM_112660.5.
UniGeneiAt.22111.
At.71032.
At.74878.
At.74926.

3D structure databases

ProteinModelPortaliQ9SCX8.
SMRiQ9SCX8. Positions 46-326.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9SCX8.
PRIDEiQ9SCX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17790.1; AT3G17790.1; AT3G17790.
GeneIDi821047.
KEGGiath:AT3G17790.

Organism-specific databases

TAIRiAT3G17790.

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000239892.
InParanoidiQ9SCX8.
OMAiVHAEVTN.
PhylomeDBiQ9SCX8.

Enzyme and pathway databases

BioCyciARA:AT3G17790-MONOMER.
ReactomeiREACT_327526. Vitamin B2 (riboflavin) metabolism.

Gene expression databases

GenevestigatoriQ9SCX8.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions."
    del Pozo J.C., Allona I., Rubio V., Leyva A., de la Pena A., Aragoncillo C., Paz-Ares J.
    Plant J. 19:579-589(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, ENZYME REGULATION, INDUCTION.
    Strain: cv. Landsberg erecta.
  2. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA17_ARATH
AccessioniPrimary (citable) accession number: Q9SCX8
Secondary accession number(s): Q8LFV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2009
Last sequence update: April 30, 2000
Last modified: March 31, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.