Reviewed, UniProtKB/Swiss-Prot Q9SCX8 (PPA17_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 17
    EC=3.1.3.2
Alternative name(s):
    Acid phosphatase type 5
    Peroxidase
    EC=1.11.1.7

Gene names

Name:	PAP17
Synonyms:	ACP5
Ordered Locus Names:	At3g17790
ORF Names:	MEB5.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metallo-phosphoesterase involved in phosphate metabolism. Has a peroxidase activity. Ref.1
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate. Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by phosphate and molibdate. Ref.1
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.2
Induction	By phosphate starvation, during senescence, by ABA, by H2O2, and by salt stress. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular phosphate ion homeostasis Ref.1 Inferred from expression pattern. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to hydrogen peroxide Ref.1 Inferred from expression pattern. Source: TAIR
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peroxidase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 338	307	Purple acid phosphatase 17		PRO_5000065319
Regions
	Region	253 – 255	3	Substrate binding By similarity
Sites
	Active site	227	1	Proton donor By similarity
	Metal binding	218	1	Zinc By similarity
	Metal binding	253	1	Zinc By similarity
	Metal binding	255	1	Iron By similarity
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	9	1	M → I in AAM61192. Ref.6
	Sequence conflict	115	1	Q → R in AAM61192. Ref.6
	Sequence conflict	245	1	G → S in AAM61192. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9SCX8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5183AE005E859740	FASTA	338	38,297
	10 20 30 40 50 60 MNSGRRSLMS ATASLSLLLC IFTTFVVVSN GELQRFIEPA KSDGSVSFIV IGDWGRRGSF 70 80 90 100 110 120 NQSLVAYQMG KIGEKIDLDF VVSTGDNFYD NGLFSEHDPN FEQSFSNIYT APSLQKQWYS 130 140 150 160 170 180 VLGNHDYRGD AEAQLSSVLR EIDSRWICLR SFVVDAELVE MFFVDTTPFV KEYYTEADGH 190 200 210 220 230 240 SYDWRAVPSR NSYVKALLRD LEVSLKSSKA RWKIVVGHHA MRSIGHHGDT KELNEELLPI 250 260 270 280 290 300 LKENGVDLYM NGHDHCLQHM SDEDSPIQFL TSGAGSKAWR GDINPVTINP KLLKFYYDGQ 310 320 330 GFMSARFTHS DAEIVFYDVF GEILHKWVTS KQLLHSSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions." del Pozo J.C., Allona I., Rubio V., Leyva A., de la Pena A., Aragoncillo C., Paz-Ares J. Plant J. 19:579-589(1999) [PubMed: 10504579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, ENZYME REGULATION, INDUCTION. Strain: cv. Landsberg erecta.
[2]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ133747 mRNA. Translation: CAC09923.1. AJ243527 Genomic DNA. Translation: CAB63938.1. AY818189 mRNA. Translation: AAV69751.1. AB019230 Genomic DNA. Translation: BAB02702.1. BT003135 mRNA. Translation: AAO24567.1. AK228106 mRNA. Translation: BAF00065.1. AY084629 mRNA. Translation: AAM61192.1.
IPI	IPI00546945.
RefSeq	NP_566587.1.
UniGene	At.22111 At.71032 Rra.13239 Rsa.1107
3D structure databases
SMR	Q9SCX8. Positions 43-331.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SCX8.
Proteomic databases
PRIDE	Q9SCX8.
Genome annotation databases
GeneID	821047.
GenomeReviews	Gene locus AT3G17790 in contig BA000014_GR.
KEGG	ath:AT3G17790.
NMPDR	fig|3702.1.peg.13974.
Organism-specific databases
TAIR	At3g17790.
Phylogenomic databases
eggNOG	KOG2679.
InParanoid	Q9SCX8.
OMA	QRSKRWN.
Gene expression databases
ArrayExpress	Q9SCX8.
Genevestigator	Q9SCX8.
Family and domain databases
InterPro	IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA17_ARATH

Accession

Primary (citable) accession number: Q9SCX8
Secondary accession number(s): Q8LFV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)