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Protein

Purple acid phosphatase 17

Gene

PAP17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metallo-phosphoesterase involved in phosphate metabolism. Has a peroxidase activity.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.
2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by phosphate and molybdate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53IronBy similarity1
Metal bindingi86IronBy similarity1
Metal bindingi86ZincBy similarity1
Metal bindingi89IronBy similarity1
Metal bindingi124ZincBy similarity1
Metal bindingi218ZincBy similarity1
Active sitei227Proton donorBy similarity1
Metal bindingi253ZincBy similarity1
Metal bindingi255IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • peroxidase activity Source: UniProtKB-EC
  • phosphatase activity Source: TAIR

GO - Biological processi

  • cellular phosphate ion homeostasis Source: TAIR
  • response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 17 (EC:3.1.3.2)
Alternative name(s):
Acid phosphatase type 5
Peroxidase (EC:1.11.1.7)
Gene namesi
Name:PAP17
Synonyms:ACP5
Ordered Locus Names:At3g17790
ORF Names:MEB5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17790.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_500006531932 – 338Purple acid phosphatase 17Add BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCX8.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Inductioni

By phosphate starvation, during senescence, by ABA, by H2O2, and by salt stress.1 Publication

Gene expression databases

GenevisibleiQ9SCX8. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G17790.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SCX8.
SMRiQ9SCX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ9SCX8.
KOiK14379.
OMAiKATAHEM.
OrthoDBiEOG09360IST.
PhylomeDBiQ9SCX8.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGRRSLMS ATASLSLLLC IFTTFVVVSN GELQRFIEPA KSDGSVSFIV
60 70 80 90 100
IGDWGRRGSF NQSLVAYQMG KIGEKIDLDF VVSTGDNFYD NGLFSEHDPN
110 120 130 140 150
FEQSFSNIYT APSLQKQWYS VLGNHDYRGD AEAQLSSVLR EIDSRWICLR
160 170 180 190 200
SFVVDAELVE MFFVDTTPFV KEYYTEADGH SYDWRAVPSR NSYVKALLRD
210 220 230 240 250
LEVSLKSSKA RWKIVVGHHA MRSIGHHGDT KELNEELLPI LKENGVDLYM
260 270 280 290 300
NGHDHCLQHM SDEDSPIQFL TSGAGSKAWR GDINPVTINP KLLKFYYDGQ
310 320 330
GFMSARFTHS DAEIVFYDVF GEILHKWVTS KQLLHSSV
Length:338
Mass (Da):38,297
Last modified:May 1, 2000 - v1
Checksum:i5183AE005E859740
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9M → I in AAM61192 (Ref. 7) Curated1
Sequence conflicti115Q → R in AAM61192 (Ref. 7) Curated1
Sequence conflicti245G → S in AAM61192 (Ref. 7) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133747 mRNA. Translation: CAC09923.1.
AJ243527 Genomic DNA. Translation: CAB63938.1.
AY818189 mRNA. Translation: AAV69751.1.
AB019230 Genomic DNA. Translation: BAB02702.1.
CP002686 Genomic DNA. Translation: AEE76007.1.
BT003135 mRNA. Translation: AAO24567.1.
AK228106 mRNA. Translation: BAF00065.1.
AY084629 mRNA. Translation: AAM61192.1.
RefSeqiNP_566587.1. NM_112660.6.
UniGeneiAt.22111.
At.71032.
At.74878.
At.74926.

Genome annotation databases

EnsemblPlantsiAT3G17790.1; AT3G17790.1; AT3G17790.
GeneIDi821047.
GrameneiAT3G17790.1; AT3G17790.1; AT3G17790.
KEGGiath:AT3G17790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133747 mRNA. Translation: CAC09923.1.
AJ243527 Genomic DNA. Translation: CAB63938.1.
AY818189 mRNA. Translation: AAV69751.1.
AB019230 Genomic DNA. Translation: BAB02702.1.
CP002686 Genomic DNA. Translation: AEE76007.1.
BT003135 mRNA. Translation: AAO24567.1.
AK228106 mRNA. Translation: BAF00065.1.
AY084629 mRNA. Translation: AAM61192.1.
RefSeqiNP_566587.1. NM_112660.6.
UniGeneiAt.22111.
At.71032.
At.74878.
At.74926.

3D structure databases

ProteinModelPortaliQ9SCX8.
SMRiQ9SCX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G17790.1.

Proteomic databases

PaxDbiQ9SCX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17790.1; AT3G17790.1; AT3G17790.
GeneIDi821047.
GrameneiAT3G17790.1; AT3G17790.1; AT3G17790.
KEGGiath:AT3G17790.

Organism-specific databases

TAIRiAT3G17790.

Phylogenomic databases

eggNOGiKOG2679. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000239892.
InParanoidiQ9SCX8.
KOiK14379.
OMAiKATAHEM.
OrthoDBiEOG09360IST.
PhylomeDBiQ9SCX8.

Enzyme and pathway databases

ReactomeiR-ATH-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

PROiQ9SCX8.

Gene expression databases

GenevisibleiQ9SCX8. AT.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA17_ARATH
AccessioniPrimary (citable) accession number: Q9SCX8
Secondary accession number(s): Q8LFV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.