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Q9SCX8 (PPA17_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 17

EC=3.1.3.2
Alternative name(s):
Acid phosphatase type 5
Peroxidase
EC=1.11.1.7
Gene names
Name:PAP17
Synonyms:ACP5
Ordered Locus Names:At3g17790
ORF Names:MEB5.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Metallo-phosphoesterase involved in phosphate metabolism. Has a peroxidase activity. Ref.1

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Inhibited by phosphate and molybdate. Ref.1

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.2

Induction

By phosphate starvation, during senescence, by ABA, by H2O2, and by salt stress. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 338307Purple acid phosphatase 17
PRO_5000065319

Regions

Region253 – 2553Substrate binding By similarity

Sites

Active site2271Proton donor By similarity
Metal binding531Iron By similarity
Metal binding861Iron By similarity
Metal binding861Zinc By similarity
Metal binding891Iron By similarity
Metal binding1241Zinc By similarity
Metal binding2181Zinc By similarity
Metal binding2531Zinc By similarity
Metal binding2551Iron By similarity

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict91M → I in AAM61192. Ref.7
Sequence conflict1151Q → R in AAM61192. Ref.7
Sequence conflict2451G → S in AAM61192. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9SCX8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5183AE005E859740

FASTA33838,297
        10         20         30         40         50         60 
MNSGRRSLMS ATASLSLLLC IFTTFVVVSN GELQRFIEPA KSDGSVSFIV IGDWGRRGSF 

        70         80         90        100        110        120 
NQSLVAYQMG KIGEKIDLDF VVSTGDNFYD NGLFSEHDPN FEQSFSNIYT APSLQKQWYS 

       130        140        150        160        170        180 
VLGNHDYRGD AEAQLSSVLR EIDSRWICLR SFVVDAELVE MFFVDTTPFV KEYYTEADGH 

       190        200        210        220        230        240 
SYDWRAVPSR NSYVKALLRD LEVSLKSSKA RWKIVVGHHA MRSIGHHGDT KELNEELLPI 

       250        260        270        280        290        300 
LKENGVDLYM NGHDHCLQHM SDEDSPIQFL TSGAGSKAWR GDINPVTINP KLLKFYYDGQ 

       310        320        330 
GFMSARFTHS DAEIVFYDVF GEILHKWVTS KQLLHSSV 

« Hide

References

« Hide 'large scale' references
[1]"A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions."
del Pozo J.C., Allona I., Rubio V., Leyva A., de la Pena A., Aragoncillo C., Paz-Ares J.
Plant J. 19:579-589(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, ENZYME REGULATION, INDUCTION.
Strain: cv. Landsberg erecta.
[2]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ133747 mRNA. Translation: CAC09923.1.
AJ243527 Genomic DNA. Translation: CAB63938.1.
AY818189 mRNA. Translation: AAV69751.1.
AB019230 Genomic DNA. Translation: BAB02702.1.
CP002686 Genomic DNA. Translation: AEE76007.1.
BT003135 mRNA. Translation: AAO24567.1.
AK228106 mRNA. Translation: BAF00065.1.
AY084629 mRNA. Translation: AAM61192.1.
RefSeqNP_566587.1. NM_112660.5.
UniGeneAt.22111.
At.71032.
At.74878.
At.74926.

3D structure databases

ProteinModelPortalQ9SCX8.
SMRQ9SCX8. Positions 46-326.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9SCX8.
PRIDEQ9SCX8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G17790.1; AT3G17790.1; AT3G17790.
GeneID821047.
KEGGath:AT3G17790.

Organism-specific databases

TAIRAT3G17790.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000239892.
InParanoidQ9SCX8.
OMATNYELIY.
PhylomeDBQ9SCX8.

Enzyme and pathway databases

BioCycARA:AT3G17790-MONOMER.

Gene expression databases

GenevestigatorQ9SCX8.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA17_ARATH
AccessionPrimary (citable) accession number: Q9SCX8
Secondary accession number(s): Q8LFV2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names