Q9SCW1 (BGAL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 1
Short name=Lactase 1
EC=3.2.1.23

Gene names

Name:	BGAL1
Ordered Locus Names:	At3g13750
ORF Names:	MMM17.1

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Ubiquitous, at low levels. Ref.6 Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	beta-galactosidase activity Inferred from direct assay. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRL1	Q9ZPS9	1	EBI-2293915,EBI-2292728

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 847	815	Beta-galactosidase 1		PRO_5000065877
Regions
	Domain	761 – 847	87	SUEL-type lectin
Sites
	Active site	190	1	Proton donor Potential
	Active site	259	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	469	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	512	1	V → L in AAM13196. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9SCW1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 91C13DE26A4CF4AD	FASTA	847	93,659
	10 20 30 40 50 60 MGSKPNAMKN VVAMAAVSAL FLLGFLVCSV SGSVSYDSRA ITINGKRRIL ISGSIHYPRS 70 80 90 100 110 120 TPEMWPDLIR KAKEGGLDVI QTYVFWNGHE PSPGKYYFEG NYDLVKFVKL VQQSGLYLHL 130 140 150 160 170 180 RIGPYVCAEW NFGGFPVWLK YIPGISFRTD NGPFKAQMQR FTTKIVNMMK AERLFESQGG 190 200 210 220 230 240 PIILSQIENE YGPMEYELGA PGRSYTNWAA KMAVGLGTGV PWVMCKQDDA PDPIINACNG 250 260 270 280 290 300 FYCDYFSPNK AYKPKMWTEA WTGWFTKFGG PVPYRPAEDM AFSVARFIQK GGSFINYYMY 310 320 330 340 350 360 HGGTNFGRTA GGPFIATSYD YDAPLDEYGL ERQPKWGHLK DLHRAIKLCE PALVSGEPTR 370 380 390 400 410 420 MPLGNYQEAH VYKSKSGACS AFLANYNPKS YAKVSFGNNH YNLPPWSISI LPDCKNTVYN 430 440 450 460 470 480 TARVGAQTSR MKMVRVPVHG GLSWQAYNED PSTYIDESFT MVGLVEQINT TRDTSDYLWY 490 500 510 520 530 540 MTDVKVDANE GFLRNGDLPT LTVLSAGHAM HVFINGQLSG SAYGSLDSPK LTFRKGVNLR 550 560 570 580 590 600 AGFNKIAILS IAVGLPNVGP HFETWNAGVL GPVSLNGLNG GRRDLSWQKW TYKVGLKGES 610 620 630 640 650 660 LSLHSLSGSS SVEWAEGAFV AQKQPLTWYK TTFSAPAGDS PLAVDMGSMG KGQIWINGQS 670 680 690 700 710 720 LGRHWPAYKA VGSCSECSYT GTFREDKCLR NCGEASQRWY HVPRSWLKPS GNLLVVFEEW 730 740 750 760 770 780 GGDPNGITLV RREVDSVCAD IYEWQSTLVN YQLHASGKVN KPLHPKAHLQ CGPGQKITTV 790 800 810 820 830 840 KFASFGTPEG TCGSYRQGSC HAHHSYDAFN KLCVGQNWCS VTVAPEMFGG DPCPNVMKKL AVEAVCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana." Gy I., Kreis M., Lecharny A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 578-847. Strain: cv. Columbia.
[6]	"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana." Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I. Plant Cell Physiol. 47:55-63(2006) [PubMed: 16267099] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ270297 mRNA. Translation: CAB64737.1. AP001307 Genomic DNA. Translation: BAB01923.1. CP002686 Genomic DNA. Translation: AEE75407.1. AY093197 mRNA. Translation: AAM13196.1. AK222229 mRNA. Translation: BAD95407.1.
IPI	IPI00516654.
RefSeq	NP_187988.1. NM_112225.4.
UniGene	At.21793. At.67729.
3D structure databases
ProteinModelPortal	Q9SCW1.
SMR	Q9SCW1. Positions 765-847.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SCW1. 1 interaction.
STRING	Q9SCW1.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q9SCW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G13750.1; AT3G13750.1; AT3G13750.
GeneID	820584.
GenomeReviews	Gene locus AT3G13750 in contig BA000014_GR.
KEGG	ath:AT3G13750.
NMPDR	fig|3702.1.peg.13492.
Organism-specific databases
GeneFarm	485. 90.
TAIR	At3g13750.
Phylogenomic databases
GeneTree	EPGT00070000027970.
HOGENOM	HBG318019.
InParanoid	Q9SCW1.
OMA	SWQAYNE.
PhylomeDB	Q9SCW1.
ProtClustDB	CLSN2684807.
Gene expression databases
Genevestigator	Q9SCW1.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS00435. PEROXIDASE_1. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL1_ARATH

Accession

Primary (citable) accession number: Q9SCW1
Secondary accession number(s): Q56W18, Q8RWC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program