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Q9SCV9 (BGAL3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 3

Short name=Lactase 3
EC=3.2.1.23
Gene names
Name:BGAL3
Ordered Locus Names:At4g36360
ORF Names:C7A10.1000, F23E13.200
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Ubiquitous. Ref.8

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence CAA18137.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB16852.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB80302.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from direct assay PubMed 19767039. Source: TAIR

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BRL1Q9ZPS91EBI-2294048,EBI-2292728

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9SCV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9SCV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     811-811: Missing.
Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 856825Beta-galactosidase 3
PRO_0000293091

Regions

Domain760 – 84687SUEL-type lectin

Sites

Active site1891Proton donor Potential
Active site2581Nucleophile Potential

Amino acid modifications

Glycosylation4681N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence8111Missing in isoform 2.
VSP_027466

Experimental info

Sequence conflict6561V → L in CAA85537. Ref.7
Sequence conflict6831K → L in CAA85537. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 245C03A09493C785

FASTA85695,193
        10         20         30         40         50         60 
MREMGTGDSA SRLILWFCLG FLILGVGFVQ CGVTYDRKAL LINGQRRILF SGSIHYPRST 

        70         80         90        100        110        120 
PDMWEDLIQK AKDGGIDVIE TYVFWNLHEP SPGKYDFEGR NDLVRFVKTI HKAGLYAHLR 

       130        140        150        160        170        180 
IGPYVCAEWN FGGFPVWLKY VPGISFRTDN EPFKRAMKGF TERIVELMKS ENLFESQGGP 

       190        200        210        220        230        240 
IILSQIENEY GRQGQLLGAE GHNYMTWAAK MAIATETGVP WVMCKEDDAP DPVINTCNGF 

       250        260        270        280        290        300 
YCDSFAPNKP YKPLIWTEAW SGWFTEFGGP MHHRPVQDLA FGVARFIQKG GSFVNYYMYH 

       310        320        330        340        350        360 
GGTNFGRTAG GPFVTTSYDY DAPIDEYGLI RQPKYGHLKE LHRAIKMCEK ALVSADPVVT 

       370        380        390        400        410        420 
SIGNKQQAHV YSAESGDCSA FLANYDTESA ARVLFNNVHY NLPPWSISIL PDCRNAVFNT 

       430        440        450        460        470        480 
AKVGVQTSQM EMLPTDTKNF QWESYLEDLS SLDDSSTFTT HGLLEQINVT RDTSDYLWYM 

       490        500        510        520        530        540 
TSVDIGDSES FLHGGELPTL IIQSTGHAVH IFVNGQLSGS AFGTRQNRRF TYQGKINLHS 

       550        560        570        580        590        600 
GTNRIALLSV AVGLPNVGGH FESWNTGILG PVALHGLSQG KMDLSWQKWT YQVGLKGEAM 

       610        620        630        640        650        660 
NLAFPTNTPS IGWMDASLTV QKPQPLTWHK TYFDAPEGNE PLALDMEGMG KGQIWVNGES 

       670        680        690        700        710        720 
IGRYWTAFAT GDCSHCSYTG TYKPNKCQTG CGQPTQRWYH VPRAWLKPSQ NLLVIFEELG 

       730        740        750        760        770        780 
GNPSTVSLVK RSVSGVCAEV SEYHPNIKNW QIESYGKGQT FHRPKVHLKC SPGQAIASIK 

       790        800        810        820        830        840 
FASFGTPLGT CGSYQQGECH AATSYAILER KCVGKARCAV TISNSNFGKD PCPNVLKRLT 

       850 
VEAVCAPETS VSTWRP 

« Hide

Isoform 2 [UniParc].

Checksum: 7314CADC0D9A9E1A
Show »

FASTA85595,065

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. expand/collapse author list , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-856 (ISOFORM 1).
Strain: cv. Columbia.
[7]"The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
Raynal M., Grellet F., Laudie M., Meyer Y., Cooke R., Delseny M.
Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 624-740.
Strain: cv. Columbia.
Tissue: Green siliques.
[8]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270299 mRNA. Translation: CAB64739.1.
Z99708 Genomic DNA. Translation: CAB16852.1. Different initiation.
AL022141 Genomic DNA. Translation: CAA18137.1. Sequence problems.
AL161589 Genomic DNA. Translation: CAB80302.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE86646.1.
CP002687 Genomic DNA. Translation: AEE86647.1.
AY056285 mRNA. Translation: AAL07134.1.
AY091432 mRNA. Translation: AAM14371.1.
AK230040 mRNA. Translation: BAF01862.1.
Z37275 mRNA. Translation: CAA85537.1.
PIRB85429.
T04600.
RefSeqNP_568001.1. NM_119799.3. [Q9SCV9-1]
NP_849506.1. NM_179175.2. [Q9SCV9-2]
UniGeneAt.703.

3D structure databases

ProteinModelPortalQ9SCV9.
SMRQ9SCV9. Positions 33-735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9SCV9. 1 interaction.
STRING3702.AT4G36360.1-P.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9SCV9.
PRIDEQ9SCV9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G36360.1; AT4G36360.1; AT4G36360. [Q9SCV9-1]
GeneID829788.
KEGGath:AT4G36360.

Organism-specific databases

GeneFarm491. 90.
TAIRAT4G36360.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9SCV9.
OMAQTSQMEM.
PhylomeDBQ9SCV9.

Enzyme and pathway databases

BioCycARA:AT4G36360-MONOMER.
ARA:GQT-587-MONOMER.

Gene expression databases

ArrayExpressQ9SCV9.
GenevestigatorQ9SCV9.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL3_ARATH
AccessionPrimary (citable) accession number: Q9SCV9
Secondary accession number(s): O23243 expand/collapse secondary AC list , Q0WLZ5, Q42150, Q42317
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names