Q9SCV9 (BGAL3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 3
Short name=Lactase 3
EC=3.2.1.23

Gene names

Name:	BGAL3
Ordered Locus Names:	At4g36360
ORF Names:	C7A10.1000, F23E13.200

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Ubiquitous. Ref.8
Sequence similarities	Belongs to the glycosyl hydrolase 35 family. Contains 1 SUEL-type lectin domain.
Sequence caution	The sequence CAA18137.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB16852.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB80302.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from direct assay. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRL1	Q9ZPS9	1	EBI-2294048,EBI-2292728

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9SCV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9SCV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     811-811: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 856	825	Beta-galactosidase 3		PRO_0000293091
Regions
	Domain	760 – 846	87	SUEL-type lectin
Sites
	Active site	189	1	Proton donor Potential
	Active site	258	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	811	1	Missing in isoform 2.		VSP_027466
Experimental info
	Sequence conflict	656	1	V → L in CAA85537. Ref.7
	Sequence conflict	683	1	K → L in CAA85537. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 245C03A09493C785	FASTA	856	95,193
	10 20 30 40 50 60 MREMGTGDSA SRLILWFCLG FLILGVGFVQ CGVTYDRKAL LINGQRRILF SGSIHYPRST 70 80 90 100 110 120 PDMWEDLIQK AKDGGIDVIE TYVFWNLHEP SPGKYDFEGR NDLVRFVKTI HKAGLYAHLR 130 140 150 160 170 180 IGPYVCAEWN FGGFPVWLKY VPGISFRTDN EPFKRAMKGF TERIVELMKS ENLFESQGGP 190 200 210 220 230 240 IILSQIENEY GRQGQLLGAE GHNYMTWAAK MAIATETGVP WVMCKEDDAP DPVINTCNGF 250 260 270 280 290 300 YCDSFAPNKP YKPLIWTEAW SGWFTEFGGP MHHRPVQDLA FGVARFIQKG GSFVNYYMYH 310 320 330 340 350 360 GGTNFGRTAG GPFVTTSYDY DAPIDEYGLI RQPKYGHLKE LHRAIKMCEK ALVSADPVVT 370 380 390 400 410 420 SIGNKQQAHV YSAESGDCSA FLANYDTESA ARVLFNNVHY NLPPWSISIL PDCRNAVFNT 430 440 450 460 470 480 AKVGVQTSQM EMLPTDTKNF QWESYLEDLS SLDDSSTFTT HGLLEQINVT RDTSDYLWYM 490 500 510 520 530 540 TSVDIGDSES FLHGGELPTL IIQSTGHAVH IFVNGQLSGS AFGTRQNRRF TYQGKINLHS 550 560 570 580 590 600 GTNRIALLSV AVGLPNVGGH FESWNTGILG PVALHGLSQG KMDLSWQKWT YQVGLKGEAM 610 620 630 640 650 660 NLAFPTNTPS IGWMDASLTV QKPQPLTWHK TYFDAPEGNE PLALDMEGMG KGQIWVNGES 670 680 690 700 710 720 IGRYWTAFAT GDCSHCSYTG TYKPNKCQTG CGQPTQRWYH VPRAWLKPSQ NLLVIFEELG 730 740 750 760 770 780 GNPSTVSLVK RSVSGVCAEV SEYHPNIKNW QIESYGKGQT FHRPKVHLKC SPGQAIASIK 790 800 810 820 830 840 FASFGTPLGT CGSYQQGECH AATSYAILER KCVGKARCAV TISNSNFGKD PCPNVLKRLT 850 VEAVCAPETS VSTWRP
	Isoform 2 [UniParc]. Checksum: 7314CADC0D9A9E1A	FASTA	855	95,065
	10 20 30 40 50 60 MREMGTGDSA SRLILWFCLG FLILGVGFVQ CGVTYDRKAL LINGQRRILF SGSIHYPRST 70 80 90 100 110 120 PDMWEDLIQK AKDGGIDVIE TYVFWNLHEP SPGKYDFEGR NDLVRFVKTI HKAGLYAHLR 130 140 150 160 170 180 IGPYVCAEWN FGGFPVWLKY VPGISFRTDN EPFKRAMKGF TERIVELMKS ENLFESQGGP 190 200 210 220 230 240 IILSQIENEY GRQGQLLGAE GHNYMTWAAK MAIATETGVP WVMCKEDDAP DPVINTCNGF 250 260 270 280 290 300 YCDSFAPNKP YKPLIWTEAW SGWFTEFGGP MHHRPVQDLA FGVARFIQKG GSFVNYYMYH 310 320 330 340 350 360 GGTNFGRTAG GPFVTTSYDY DAPIDEYGLI RQPKYGHLKE LHRAIKMCEK ALVSADPVVT 370 380 390 400 410 420 SIGNKQQAHV YSAESGDCSA FLANYDTESA ARVLFNNVHY NLPPWSISIL PDCRNAVFNT 430 440 450 460 470 480 AKVGVQTSQM EMLPTDTKNF QWESYLEDLS SLDDSSTFTT HGLLEQINVT RDTSDYLWYM 490 500 510 520 530 540 TSVDIGDSES FLHGGELPTL IIQSTGHAVH IFVNGQLSGS AFGTRQNRRF TYQGKINLHS 550 560 570 580 590 600 GTNRIALLSV AVGLPNVGGH FESWNTGILG PVALHGLSQG KMDLSWQKWT YQVGLKGEAM 610 620 630 640 650 660 NLAFPTNTPS IGWMDASLTV QKPQPLTWHK TYFDAPEGNE PLALDMEGMG KGQIWVNGES 670 680 690 700 710 720 IGRYWTAFAT GDCSHCSYTG TYKPNKCQTG CGQPTQRWYH VPRAWLKPSQ NLLVIFEELG 730 740 750 760 770 780 GNPSTVSLVK RSVSGVCAEV SEYHPNIKNW QIESYGKGQT FHRPKVHLKC SPGQAIASIK 790 800 810 820 830 840 FASFGTPLGT CGSYQQGECH AATSYAILER CVGKARCAVT ISNSNFGKDP CPNVLKRLTV 850 EAVCAPETSV STWRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana." Gy I., Kreis M., Lecharny A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-856 (ISOFORM 1). Strain: cv. Columbia.
[7]	"The Arabidopsis thaliana transcribed genome: the GDR cDNA program." Raynal M., Grellet F., Laudie M., Meyer Y., Cooke R., Delseny M. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 624-740. Strain: cv. Columbia. Tissue: Green siliques.
[8]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ270299 mRNA. Translation: CAB64739.1. Z99708 Genomic DNA. Translation: CAB16852.1. Different initiation. AL022141 Genomic DNA. Translation: CAA18137.1. Sequence problems. AL161589 Genomic DNA. Translation: CAB80302.1. Different initiation. CP002687 Genomic DNA. Translation: AEE86646.1. CP002687 Genomic DNA. Translation: AEE86647.1. AY056285 mRNA. Translation: AAL07134.1. AY091432 mRNA. Translation: AAM14371.1. AK230040 mRNA. Translation: BAF01862.1. Z37275 mRNA. Translation: CAA85537.1.
IPI	IPI00539409. IPI00547588.
PIR	B85429. T04600.
RefSeq	NP_568001.1. NM_119799.3. NP_849506.1. NM_179175.2.
UniGene	At.703.
3D structure databases
ProteinModelPortal	Q9SCV9.
SMR	Q9SCV9. Positions 59-147, 763-846.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9SCV9. 1 interaction.
STRING	Q9SCV9.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q9SCV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G36360.1; AT4G36360.1; AT4G36360.
GeneID	829788.
GenomeReviews	Gene locus AT4G36360 in contig CT486007_GR.
KEGG	ath:AT4G36360.
NMPDR	fig|3702.1.peg.21729.
Organism-specific databases
GeneFarm	491. 90.
TAIR	At4g36360.
Phylogenomic databases
eggNOG	KOG0496.
GeneTree	EPGT00070000027970.
HOGENOM	HBG318019.
InParanoid	Q9SCV9.
OMA	LPNVGGH.
PhylomeDB	Q9SCV9.
ProtClustDB	CLSN2689785.
Gene expression databases
Genevestigator	Q9SCV9.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. IPR000922. Lectin_gal-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02140. Gal_Lectin. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. PS50228. SUEL_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL3_ARATH

Accession

Primary (citable) accession number: Q9SCV9
Secondary accession number(s): O23243 , Q0WLZ5, Q42150, Q42317

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program