Reviewed, UniProtKB/Swiss-Prot Q9SCV8 (BGAL4_ARATH)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 4
      Short name=Lactase 4
    EC=3.2.1.23

Gene names

Name:	BGAL4
Ordered Locus Names:	At5g56870
ORF Names:	MPI10.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Preferentially hydrolyzes para-nitrophenyl-beta-D-galactoside. Can hydrolyzes para-nitrophenyl-beta-D-fucoside with 5 time less efficiency. Ref.6
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Probable.
Tissue specificity	Ubiquitous, with higher expression levels in roots and siliques. Ref.6 Ref.5 Ref.7
Induction	By sugar starvation and exposure to darkness for 24 hours. Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.
Biophysicochemical properties	Kinetic parameters: Measured at pH 4.5 and 37 degrees Celsius for all experiments. KM=3.4 µM for para-nitrophenyl-beta-D-galactoside KM=2.2 µM for ortho-nitrophenyl-beta-D-galactoside Vmax=467 nmol/sec/mg enzyme with para-nitrophenyl-beta-D-galactoside as substrate Vmax=1630 nmol/sec/mg enzyme with ortho-nitrophenyl-beta-D-galactoside as substrate

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Traceable author statement. Source: TAIR cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 724	697	Beta-galactosidase 4		PRO_5000065879
Sites
	Active site	185	1	Proton donor Potential
	Active site	254	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	255	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	290	1	V → I in AAK96780. Ref.3
	Sequence conflict	290	1	V → I in AAL47393. Ref.3
	Sequence conflict	561	1	V → A in BAD94714. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9SCV8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 57D314FEA3C9861D	FASTA	724	80,591
	10 20 30 40 50 60 MVLNFRDKSC IFLAILCCLS LSCIVKASVS YDRKAVIING QRRILLSGSI HYPRSTPEMW 70 80 90 100 110 120 PGLIQKAKEG GLDVIETYVF WNGHEPSPGQ YYFGDRYDLV KFIKLVHQAG LYVNLRIGPY 130 140 150 160 170 180 VCAEWNFGGF PVWLKFVPGM AFRTDNEPFK AAMKKFTEKI VWMMKAEKLF QTQGGPIILA 190 200 210 220 230 240 QIENEYGPVE WEIGAPGKAY TKWVAQMALG LSTGVPWIMC KQEDAPGPII DTCNGYYCED 250 260 270 280 290 300 FKPNSINKPK MWTENWTGWY TDFGGAVPYR PVEDIAYSVA RFIQKGGSLV NYYMYHGGTN 310 320 330 340 350 360 FDRTAGEFMA SSYDYDAPLD EYGLPREPKY SHLKALHKAI KLSEPALLSA DATVTSLGAK 370 380 390 400 410 420 QEAYVFWSKS SCAAFLSNKD ENSAARVLFR GFPYDLPPWS VSILPDCKTE VYNTAKVNAP 430 440 450 460 470 480 SVHRNMVPTG TKFSWGSFNE ATPTANEAGT FARNGLVEQI SMTWDKSDYF WYITDITIGS 490 500 510 520 530 540 GETFLKTGDS PLLTVMSAGH ALHVFVNGQL SGTAYGGLDH PKLTFSQKIK LHAGVNKIAL 550 560 570 580 590 600 LSVAVGLPNV GTHFEQWNKG VLGPVTLKGV NSGTWDMSKW KWSYKIGVKG EALSLHTNTE 610 620 630 640 650 660 SSGVRWTQGS FVAKKQPLTW YKSTFATPAG NEPLALDMNT MGKGQVWING RNIGRHWPAY 670 680 690 700 710 720 KAQGSCGRCN YAGTFDAKKC LSNCGEASQR WYHVPRSWLK SQNLIVVFEE LGGDPNGISL VKRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana." Gy I., Kreis M., Lecharny A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 526-724. Strain: cv. Columbia.
[5]	"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana." Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I. Plant Cell Physiol. 47:55-63(2006) [PubMed: 16267099] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[7]	"Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis." Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N. Plant Cell Physiol. 48:405-413(2007) [PubMed: 17234672] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ270300 mRNA. Translation: CAB64740.1. AB020747 Genomic DNA. Translation: BAA97206.1. AY054589 mRNA. Translation: AAK96780.1. AY064690 mRNA. Translation: AAL47393.1. AK222026 mRNA. Translation: BAD94714.1.
IPI	IPI00530701.
RefSeq	NP_200498.1.
UniGene	At.21539
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9SCV8.
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q9SCV8.
Genome annotation databases
GeneID	835789.
GenomeReviews	Gene locus AT5G56870 in contig BA000015_GR.
KEGG	ath:AT5G56870.
NMPDR	fig|3702.1.peg.27627.
Organism-specific databases
GeneFarm	493. 90.
TAIR	At5g56870.
Phylogenomic databases
OMA	VENEYGP.
Enzyme and pathway databases
BRENDA	3.2.1.23. 302.
Gene expression databases
Genevestigator	Q9SCV8.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL4_ARATH

Accession

Primary (citable) accession number: Q9SCV8
Secondary accession number(s): Q56WL4, Q93Y27

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)