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Q9SCV8

- BGAL4_ARATH

UniProt

Q9SCV8 - BGAL4_ARATH

Protein

Beta-galactosidase 4

Gene

BGAL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Preferentially hydrolyzes para-nitrophenyl-beta-D-galactoside. Can hydrolyze para-nitrophenyl-beta-D-fucoside with 5 time less efficiency.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Kineticsi

    Measured at pH 4.5 and 37 degrees Celsius for all experiments.

    1. KM=3.4 µM for para-nitrophenyl-beta-D-galactoside1 Publication
    2. KM=2.2 µM for ortho-nitrophenyl-beta-D-galactoside1 Publication

    Vmax=467 nmol/sec/mg enzyme with para-nitrophenyl-beta-D-galactoside as substrate1 Publication

    Vmax=1630 nmol/sec/mg enzyme with ortho-nitrophenyl-beta-D-galactoside as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei185 – 1851Proton donorSequence Analysis
    Active sitei254 – 2541NucleophileSequence Analysis

    GO - Molecular functioni

    1. beta-galactosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G56870-MONOMER.
    SABIO-RKQ9SCV8.

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase 4 (EC:3.2.1.23)
    Short name:
    Lactase 4
    Gene namesi
    Name:BGAL4
    Ordered Locus Names:At5g56870
    ORF Names:MPI10.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56870.

    Subcellular locationi

    Secretedextracellular spaceapoplast 1 Publication

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 724697Beta-galactosidase 4PRO_5000065879Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9SCV8.
    PRIDEiQ9SCV8.

    Expressioni

    Tissue specificityi

    Ubiquitous, with higher expression levels in roots and siliques.3 Publications

    Inductioni

    By sugar starvation and exposure to darkness for 24 hours.1 Publication

    Gene expression databases

    GenevestigatoriQ9SCV8.

    Interactioni

    Protein-protein interaction databases

    BioGridi21033. 1 interaction.
    STRINGi3702.AT5G56870.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SCV8.
    SMRiQ9SCV8. Positions 28-724.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000239919.
    InParanoidiQ9SCV8.
    OMAiWSKSSCA.
    PhylomeDBiQ9SCV8.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SCV8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLNFRDKSC IFLAILCCLS LSCIVKASVS YDRKAVIING QRRILLSGSI    50
    HYPRSTPEMW PGLIQKAKEG GLDVIETYVF WNGHEPSPGQ YYFGDRYDLV 100
    KFIKLVHQAG LYVNLRIGPY VCAEWNFGGF PVWLKFVPGM AFRTDNEPFK 150
    AAMKKFTEKI VWMMKAEKLF QTQGGPIILA QIENEYGPVE WEIGAPGKAY 200
    TKWVAQMALG LSTGVPWIMC KQEDAPGPII DTCNGYYCED FKPNSINKPK 250
    MWTENWTGWY TDFGGAVPYR PVEDIAYSVA RFIQKGGSLV NYYMYHGGTN 300
    FDRTAGEFMA SSYDYDAPLD EYGLPREPKY SHLKALHKAI KLSEPALLSA 350
    DATVTSLGAK QEAYVFWSKS SCAAFLSNKD ENSAARVLFR GFPYDLPPWS 400
    VSILPDCKTE VYNTAKVNAP SVHRNMVPTG TKFSWGSFNE ATPTANEAGT 450
    FARNGLVEQI SMTWDKSDYF WYITDITIGS GETFLKTGDS PLLTVMSAGH 500
    ALHVFVNGQL SGTAYGGLDH PKLTFSQKIK LHAGVNKIAL LSVAVGLPNV 550
    GTHFEQWNKG VLGPVTLKGV NSGTWDMSKW KWSYKIGVKG EALSLHTNTE 600
    SSGVRWTQGS FVAKKQPLTW YKSTFATPAG NEPLALDMNT MGKGQVWING 650
    RNIGRHWPAY KAQGSCGRCN YAGTFDAKKC LSNCGEASQR WYHVPRSWLK 700
    SQNLIVVFEE LGGDPNGISL VKRT 724
    Length:724
    Mass (Da):80,591
    Last modified:May 1, 2000 - v1
    Checksum:i57D314FEA3C9861D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti290 – 2901V → I in AAK96780. (PubMed:14593172)Curated
    Sequence conflicti290 – 2901V → I in AAL47393. (PubMed:14593172)Curated
    Sequence conflicti561 – 5611V → A in BAD94714. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ270300 mRNA. Translation: CAB64740.1.
    AB020747 Genomic DNA. Translation: BAA97206.1.
    CP002688 Genomic DNA. Translation: AED96817.1.
    AY054589 mRNA. Translation: AAK96780.1.
    AY064690 mRNA. Translation: AAL47393.1.
    AK222026 mRNA. Translation: BAD94714.1.
    RefSeqiNP_200498.1. NM_125070.2.
    UniGeneiAt.21539.

    Genome annotation databases

    EnsemblPlantsiAT5G56870.1; AT5G56870.1; AT5G56870.
    GeneIDi835789.
    KEGGiath:AT5G56870.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ270300 mRNA. Translation: CAB64740.1 .
    AB020747 Genomic DNA. Translation: BAA97206.1 .
    CP002688 Genomic DNA. Translation: AED96817.1 .
    AY054589 mRNA. Translation: AAK96780.1 .
    AY064690 mRNA. Translation: AAL47393.1 .
    AK222026 mRNA. Translation: BAD94714.1 .
    RefSeqi NP_200498.1. NM_125070.2.
    UniGenei At.21539.

    3D structure databases

    ProteinModelPortali Q9SCV8.
    SMRi Q9SCV8. Positions 28-724.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21033. 1 interaction.
    STRINGi 3702.AT5G56870.1-P.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Proteomic databases

    PaxDbi Q9SCV8.
    PRIDEi Q9SCV8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56870.1 ; AT5G56870.1 ; AT5G56870 .
    GeneIDi 835789.
    KEGGi ath:AT5G56870.

    Organism-specific databases

    GeneFarmi 493. 90.
    TAIRi AT5G56870.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000239919.
    InParanoidi Q9SCV8.
    OMAi WSKSSCA.
    PhylomeDBi Q9SCV8.

    Enzyme and pathway databases

    BioCyci ARA:AT5G56870-MONOMER.
    SABIO-RK Q9SCV8.

    Gene expression databases

    Genevestigatori Q9SCV8.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
      Gy I., Kreis M., Lecharny A.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 526-724.
      Strain: cv. Columbia.
    6. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
      Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
      Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
      Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
      Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    8. "Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
      Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
      Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiBGAL4_ARATH
    AccessioniPrimary (citable) accession number: Q9SCV8
    Secondary accession number(s): Q56WL4, Q93Y27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3