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Protein

Beta-galactosidase 4

Gene

BGAL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Preferentially hydrolyzes para-nitrophenyl-beta-D-galactoside. Can hydrolyze para-nitrophenyl-beta-D-fucoside with 5 time less efficiency.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Kineticsi

Measured at pH 4.5 and 37 degrees Celsius for all experiments.
  1. KM=3.4 µM for para-nitrophenyl-beta-D-galactoside1 Publication
  2. KM=2.2 µM for ortho-nitrophenyl-beta-D-galactoside1 Publication
  1. Vmax=467 nmol/sec/mg enzyme with para-nitrophenyl-beta-D-galactoside as substrate1 Publication
  2. Vmax=1630 nmol/sec/mg enzyme with ortho-nitrophenyl-beta-D-galactoside as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Proton donorSequence analysis1
Active sitei254NucleophileSequence analysis1

GO - Molecular functioni

  • beta-galactosidase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G56870-MONOMER
SABIO-RKiQ9SCV8

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 4 (EC:3.2.1.23)
Short name:
Lactase 4
Gene namesi
Name:BGAL4
Ordered Locus Names:At5g56870
ORF Names:MPI10.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56870
TAIRilocus:2170282 AT5G56870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_500006587928 – 724Beta-galactosidase 4Add BLAST697

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi255N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV8
PRIDEiQ9SCV8

Expressioni

Tissue specificityi

Ubiquitous, with higher expression levels in roots and siliques.3 Publications

Inductioni

By sugar starvation and exposure to darkness for 24 hours.1 Publication

Gene expression databases

ExpressionAtlasiQ9SCV8 baseline and differential
GenevisibleiQ9SCV8 AT

Interactioni

Protein-protein interaction databases

BioGridi21033, 1 interactor
STRINGi3702.AT5G56870.1

Structurei

3D structure databases

ProteinModelPortaliQ9SCV8
SMRiQ9SCV8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496 Eukaryota
COG1874 LUCA
HOGENOMiHOG000239919
InParanoidiQ9SCV8
OMAiWGSFNEA
OrthoDBiEOG093601RS
PhylomeDBiQ9SCV8

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF01301 Glyco_hydro_35, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SUPFAMiSSF49785 SSF49785, 3 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLNFRDKSC IFLAILCCLS LSCIVKASVS YDRKAVIING QRRILLSGSI
60 70 80 90 100
HYPRSTPEMW PGLIQKAKEG GLDVIETYVF WNGHEPSPGQ YYFGDRYDLV
110 120 130 140 150
KFIKLVHQAG LYVNLRIGPY VCAEWNFGGF PVWLKFVPGM AFRTDNEPFK
160 170 180 190 200
AAMKKFTEKI VWMMKAEKLF QTQGGPIILA QIENEYGPVE WEIGAPGKAY
210 220 230 240 250
TKWVAQMALG LSTGVPWIMC KQEDAPGPII DTCNGYYCED FKPNSINKPK
260 270 280 290 300
MWTENWTGWY TDFGGAVPYR PVEDIAYSVA RFIQKGGSLV NYYMYHGGTN
310 320 330 340 350
FDRTAGEFMA SSYDYDAPLD EYGLPREPKY SHLKALHKAI KLSEPALLSA
360 370 380 390 400
DATVTSLGAK QEAYVFWSKS SCAAFLSNKD ENSAARVLFR GFPYDLPPWS
410 420 430 440 450
VSILPDCKTE VYNTAKVNAP SVHRNMVPTG TKFSWGSFNE ATPTANEAGT
460 470 480 490 500
FARNGLVEQI SMTWDKSDYF WYITDITIGS GETFLKTGDS PLLTVMSAGH
510 520 530 540 550
ALHVFVNGQL SGTAYGGLDH PKLTFSQKIK LHAGVNKIAL LSVAVGLPNV
560 570 580 590 600
GTHFEQWNKG VLGPVTLKGV NSGTWDMSKW KWSYKIGVKG EALSLHTNTE
610 620 630 640 650
SSGVRWTQGS FVAKKQPLTW YKSTFATPAG NEPLALDMNT MGKGQVWING
660 670 680 690 700
RNIGRHWPAY KAQGSCGRCN YAGTFDAKKC LSNCGEASQR WYHVPRSWLK
710 720
SQNLIVVFEE LGGDPNGISL VKRT
Length:724
Mass (Da):80,591
Last modified:May 1, 2000 - v1
Checksum:i57D314FEA3C9861D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290V → I in AAK96780 (PubMed:14593172).Curated1
Sequence conflicti290V → I in AAL47393 (PubMed:14593172).Curated1
Sequence conflicti561V → A in BAD94714 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270300 mRNA Translation: CAB64740.1
AB020747 Genomic DNA Translation: BAA97206.1
CP002688 Genomic DNA Translation: AED96817.1
AY054589 mRNA Translation: AAK96780.1
AY064690 mRNA Translation: AAL47393.1
AK222026 mRNA Translation: BAD94714.1
RefSeqiNP_200498.1, NM_125070.3
UniGeneiAt.21539

Genome annotation databases

EnsemblPlantsiAT5G56870.1; AT5G56870.1; AT5G56870
GeneIDi835789
GrameneiAT5G56870.1; AT5G56870.1; AT5G56870
KEGGiath:AT5G56870

Similar proteinsi

Entry informationi

Entry nameiBGAL4_ARATH
AccessioniPrimary (citable) accession number: Q9SCV8
Secondary accession number(s): Q56WL4, Q93Y27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health