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Q9SCV8

- BGAL4_ARATH

UniProt

Q9SCV8 - BGAL4_ARATH

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Protein

Beta-galactosidase 4

Gene

BGAL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Preferentially hydrolyzes para-nitrophenyl-beta-D-galactoside. Can hydrolyze para-nitrophenyl-beta-D-fucoside with 5 time less efficiency.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Kineticsi

Measured at pH 4.5 and 37 degrees Celsius for all experiments.

  1. KM=3.4 µM for para-nitrophenyl-beta-D-galactoside1 Publication
  2. KM=2.2 µM for ortho-nitrophenyl-beta-D-galactoside1 Publication

Vmax=467 nmol/sec/mg enzyme with para-nitrophenyl-beta-D-galactoside as substrate1 Publication

Vmax=1630 nmol/sec/mg enzyme with ortho-nitrophenyl-beta-D-galactoside as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei185 – 1851Proton donorSequence Analysis
Active sitei254 – 2541NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G56870-MONOMER.
SABIO-RKQ9SCV8.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 4 (EC:3.2.1.23)
Short name:
Lactase 4
Gene namesi
Name:BGAL4
Ordered Locus Names:At5g56870
ORF Names:MPI10.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G56870.

Subcellular locationi

Secretedextracellular spaceapoplast 1 Publication

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 724697Beta-galactosidase 4PRO_5000065879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV8.
PRIDEiQ9SCV8.

Expressioni

Tissue specificityi

Ubiquitous, with higher expression levels in roots and siliques.3 Publications

Inductioni

By sugar starvation and exposure to darkness for 24 hours.1 Publication

Gene expression databases

GenevestigatoriQ9SCV8.

Interactioni

Protein-protein interaction databases

BioGridi21033. 1 interaction.
STRINGi3702.AT5G56870.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SCV8.
SMRiQ9SCV8. Positions 28-724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV8.
OMAiWSKSSCA.
PhylomeDBiQ9SCV8.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SCV8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLNFRDKSC IFLAILCCLS LSCIVKASVS YDRKAVIING QRRILLSGSI
60 70 80 90 100
HYPRSTPEMW PGLIQKAKEG GLDVIETYVF WNGHEPSPGQ YYFGDRYDLV
110 120 130 140 150
KFIKLVHQAG LYVNLRIGPY VCAEWNFGGF PVWLKFVPGM AFRTDNEPFK
160 170 180 190 200
AAMKKFTEKI VWMMKAEKLF QTQGGPIILA QIENEYGPVE WEIGAPGKAY
210 220 230 240 250
TKWVAQMALG LSTGVPWIMC KQEDAPGPII DTCNGYYCED FKPNSINKPK
260 270 280 290 300
MWTENWTGWY TDFGGAVPYR PVEDIAYSVA RFIQKGGSLV NYYMYHGGTN
310 320 330 340 350
FDRTAGEFMA SSYDYDAPLD EYGLPREPKY SHLKALHKAI KLSEPALLSA
360 370 380 390 400
DATVTSLGAK QEAYVFWSKS SCAAFLSNKD ENSAARVLFR GFPYDLPPWS
410 420 430 440 450
VSILPDCKTE VYNTAKVNAP SVHRNMVPTG TKFSWGSFNE ATPTANEAGT
460 470 480 490 500
FARNGLVEQI SMTWDKSDYF WYITDITIGS GETFLKTGDS PLLTVMSAGH
510 520 530 540 550
ALHVFVNGQL SGTAYGGLDH PKLTFSQKIK LHAGVNKIAL LSVAVGLPNV
560 570 580 590 600
GTHFEQWNKG VLGPVTLKGV NSGTWDMSKW KWSYKIGVKG EALSLHTNTE
610 620 630 640 650
SSGVRWTQGS FVAKKQPLTW YKSTFATPAG NEPLALDMNT MGKGQVWING
660 670 680 690 700
RNIGRHWPAY KAQGSCGRCN YAGTFDAKKC LSNCGEASQR WYHVPRSWLK
710 720
SQNLIVVFEE LGGDPNGISL VKRT
Length:724
Mass (Da):80,591
Last modified:May 1, 2000 - v1
Checksum:i57D314FEA3C9861D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti290 – 2901V → I in AAK96780. (PubMed:14593172)Curated
Sequence conflicti290 – 2901V → I in AAL47393. (PubMed:14593172)Curated
Sequence conflicti561 – 5611V → A in BAD94714. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270300 mRNA. Translation: CAB64740.1.
AB020747 Genomic DNA. Translation: BAA97206.1.
CP002688 Genomic DNA. Translation: AED96817.1.
AY054589 mRNA. Translation: AAK96780.1.
AY064690 mRNA. Translation: AAL47393.1.
AK222026 mRNA. Translation: BAD94714.1.
RefSeqiNP_200498.1. NM_125070.2.
UniGeneiAt.21539.

Genome annotation databases

EnsemblPlantsiAT5G56870.1; AT5G56870.1; AT5G56870.
GeneIDi835789.
KEGGiath:AT5G56870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270300 mRNA. Translation: CAB64740.1 .
AB020747 Genomic DNA. Translation: BAA97206.1 .
CP002688 Genomic DNA. Translation: AED96817.1 .
AY054589 mRNA. Translation: AAK96780.1 .
AY064690 mRNA. Translation: AAL47393.1 .
AK222026 mRNA. Translation: BAD94714.1 .
RefSeqi NP_200498.1. NM_125070.2.
UniGenei At.21539.

3D structure databases

ProteinModelPortali Q9SCV8.
SMRi Q9SCV8. Positions 28-724.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21033. 1 interaction.
STRINGi 3702.AT5G56870.1-P.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbi Q9SCV8.
PRIDEi Q9SCV8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G56870.1 ; AT5G56870.1 ; AT5G56870 .
GeneIDi 835789.
KEGGi ath:AT5G56870.

Organism-specific databases

GeneFarmi 493. 90.
TAIRi AT5G56870.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000239919.
InParanoidi Q9SCV8.
OMAi WSKSSCA.
PhylomeDBi Q9SCV8.

Enzyme and pathway databases

BioCyci ARA:AT5G56870-MONOMER.
SABIO-RK Q9SCV8.

Gene expression databases

Genevestigatori Q9SCV8.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 526-724.
    Strain: cv. Columbia.
  6. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  8. "Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis."
    Lee E.-J., Matsumura Y., Soga K., Hoson T., Koizumi N.
    Plant Cell Physiol. 48:405-413(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiBGAL4_ARATH
AccessioniPrimary (citable) accession number: Q9SCV8
Secondary accession number(s): Q56WL4, Q93Y27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3