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Q9SCV5 (BGAL7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 7

Short name=Lactase 7
EC=3.2.1.23
Gene names
Name:BGAL7
Ordered Locus Names:At5g20710
ORF Names:T1M15.110
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Expressed in flowers. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence AAK76465.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Traceable author statement PubMed 15517348. Source: TAIR

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 826801Beta-galactosidase 7
PRO_0000293092

Regions

Domain740 – 82687SUEL-type lectin

Sites

Active site1841Proton donor Potential
Active site2531Nucleophile Potential

Amino acid modifications

Glycosylation1541N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Glycosylation3801N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Glycosylation6651N-linked (GlcNAc...) Potential
Glycosylation7061N-linked (GlcNAc...) Potential
Glycosylation7971N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6741E → K in AAK76465. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9SCV5 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 3EDA7EBA3E5D234B

FASTA82692,712
        10         20         30         40         50         60 
MKMKHFTRLL SLFFILITSL SLAKSTIVSH DERAITINGK RRILLSGSIH YPRSTADMWP 

        70         80         90        100        110        120 
DLINKAKDGG LDAIETYVFW NAHEPKRREY DFSGNLDVVR FIKTIQDAGL YSVLRIGPYV 

       130        140        150        160        170        180 
CAEWNYGGFP VWLHNMPNMK FRTVNPSFMN EMQNFTTKIV KMMKEEKLFA SQGGPIILAQ 

       190        200        210        220        230        240 
IENEYGNVIS SYGAEGKAYI DWCANMANSL DIGVPWLMCQ QPNAPQPMLE TCNGFYCDQY 

       250        260        270        280        290        300 
EPTNPSTPKM WTENWTGWFK NWGGKHPYRT AEDLAFSVAR FFQTGGTFQN YYMYHGGTNF 

       310        320        330        340        350        360 
GRVAGGPYIT TSYDYHAPLD EFGNLNQPKW GHLKQLHTVL KSMEKSLTYG NISRIDLGNS 

       370        380        390        400        410        420 
IKATIYTTKE GSSCFIGNVN ATADALVNFK GKDYHVPAWS VSVLPDCDKE AYNTAKVNTQ 

       430        440        450        460        470        480 
TSIMTEDSSK PERLEWTWRP ESAQKMILKG SGDLIAKGLV DQKDVTNDAS DYLWYMTRLH 

       490        500        510        520        530        540 
LDKKDPLWSR NMTLRVHSNA HVLHAYVNGK YVGNQFVKDG KFDYRFERKV NHLVHGTNHI 

       550        560        570        580        590        600 
SLLSVSVGLQ NYGPFFESGP TGINGPVSLV GYKGEETIEK DLSQHQWDYK IGLNGYNDKL 

       610        620        630        640        650        660 
FSIKSVGHQK WANEKLPTGR MLTWYKAKFK APLGKEPVIV DLNGLGKGEA WINGQSIGRY 

       670        680        690        700        710        720 
WPSFNSSDDG CKDECDYRGA YGSDKCAFMC GKPTQRWYHV PRSFLNASGH NTITLFEEMG 

       730        740        750        760        770        780 
GNPSMVNFKT VVVGTVCARA HEHNKVELSC HNRPISAVKF ASFGNPLGHC GSFAVGTCQG 

       790        800        810        820 
DKDAAKTVAK ECVGKLNCTV NVSSDTFGST LDCGDSPKKL AVELEC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-826.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-826.
Strain: cv. Columbia.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF296832 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92880.1.
AJ270303 mRNA. Translation: CAB64743.1.
AY045791 mRNA. Translation: AAK76465.1. Different initiation.
RefSeqNP_568399.4. NM_122078.4.
UniGeneAt.22291.

3D structure databases

ProteinModelPortalQ9SCV5.
SMRQ9SCV5. Positions 24-735, 740-826.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9SCV5.
PRIDEQ9SCV5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G20710.1; AT5G20710.1; AT5G20710.
GeneID832194.
KEGGath:AT5G20710.

Organism-specific databases

GeneFarm534. 90.
TAIRAT5G20710.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9SCV5.
OMAHDERAIT.
PhylomeDBQ9SCV5.
ProtClustDBCLSN2918868.

Enzyme and pathway databases

BioCycARA:AT5G20710-MONOMER.

Gene expression databases

GenevestigatorQ9SCV5.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL7_ARATH
AccessionPrimary (citable) accession number: Q9SCV5
Secondary accession number(s): Q94AU4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: March 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names