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Q9SCV4

- BGAL8_ARATH

UniProt

Q9SCV4 - BGAL8_ARATH

Protein

Beta-galactosidase 8

Gene

BGAL8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei188 – 1881Proton donorSequence Analysis
    Active sitei257 – 2571NucleophileSequence Analysis

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G28470-MONOMER.
    ARA:GQT-1273-MONOMER.

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase 8 (EC:3.2.1.23)
    Short name:
    Lactase 8
    Alternative name(s):
    Protein AR782
    Gene namesi
    Name:BGAL8
    Ordered Locus Names:At2g28470
    ORF Names:T17D12.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G28470.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 852823Beta-galactosidase 8PRO_5000065882Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi766 – 7661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi807 – 8071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9SCV4.
    PRIDEiQ9SCV4.

    Expressioni

    Tissue specificityi

    Expressed in roots, flowers and siliques.2 Publications

    Gene expression databases

    ArrayExpressiQ9SCV4.
    GenevestigatoriQ9SCV4.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G28470.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SCV4.
    SMRiQ9SCV4. Positions 31-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini766 – 85287SUEL-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated
    Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000239919.
    InParanoidiQ9SCV4.
    OMAiNTAQINS.
    PhylomeDBiQ9SCV4.

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9SCV4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEIAAKMVKV RKMEMILLLI LVIVVAATAA NVTYDHRALV IDGKRKVLIS    50
    GSIHYPRSTP EMWPELIQKS KDGGLDVIET YVFWSGHEPE KNKYNFEGRY 100
    DLVKFVKLAA KAGLYVHLRI GPYVCAEWNY GGFPVWLHFV PGIKFRTDNE 150
    PFKEEMQRFT TKIVDLMKQE KLYASQGGPI ILSQIENEYG NIDSAYGAAA 200
    KSYIKWSASM ALSLDTGVPW NMCQQTDAPD PMINTCNGFY CDQFTPNSNN 250
    KPKMWTENWS GWFLGFGDPS PYRPVEDLAF AVARFYQRGG TFQNYYMYHG 300
    GTNFDRTSGG PLISTSYDYD APIDEYGLLR QPKWGHLRDL HKAIKLCEDA 350
    LIATDPTITS LGSNLEAAVY KTESGSCAAF LANVDTKSDA TVTFNGKSYN 400
    LPAWSVSILP DCKNVAFNTA KINSATESTA FARQSLKPDG GSSAELGSQW 450
    SYIKEPIGIS KADAFLKPGL LEQINTTADK SDYLWYSLRT DIKGDETFLD 500
    EGSKAVLHIE SLGQVVYAFI NGKLAGSGHG KQKISLDIPI NLVTGTNTID 550
    LLSVTVGLAN YGAFFDLVGA GITGPVTLKS AKGGSSIDLA SQQWTYQVGL 600
    KGEDTGLATV DSSEWVSKSP LPTKQPLIWY KTTFDAPSGS EPVAIDFTGT 650
    GKGIAWVNGQ SIGRYWPTSI AGNGGCTESC DYRGSYRANK CLKNCGKPSQ 700
    TLYHVPRSWL KPSGNILVLF EEMGGDPTQI SFATKQTGSN LCLTVSQSHP 750
    PPVDTWTSDS KISNRNRTRP VLSLKCPIST QVIFSIKFAS FGTPKGTCGS 800
    FTQGHCNSSR SLSLVQKACI GLRSCNVEVS TRVFGEPCRG VVKSLAVEAS 850
    CS 852
    Length:852
    Mass (Da):93,210
    Last modified:July 10, 2007 - v2
    Checksum:i54DCFB09790EE045
    GO

    Sequence cautioni

    The sequence AAD21482.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti568 – 5681V → M in CAB64744. 1 PublicationCurated
    Sequence conflicti647 – 6482FT → TA in BAA13685. (PubMed:9287109)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ270304 mRNA. Translation: CAB64744.1.
    AC006587 Genomic DNA. Translation: AAD21482.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08127.1.
    D88744 mRNA. Translation: BAA13685.1.
    PIRiC84685.
    RefSeqiNP_850121.1. NM_179790.2. [Q9SCV4-1]
    UniGeneiAt.20550.
    At.66398.

    Genome annotation databases

    EnsemblPlantsiAT2G28470.1; AT2G28470.1; AT2G28470. [Q9SCV4-1]
    GeneIDi817395.
    KEGGiath:AT2G28470.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ270304 mRNA. Translation: CAB64744.1 .
    AC006587 Genomic DNA. Translation: AAD21482.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC08127.1 .
    D88744 mRNA. Translation: BAA13685.1 .
    PIRi C84685.
    RefSeqi NP_850121.1. NM_179790.2. [Q9SCV4-1 ]
    UniGenei At.20550.
    At.66398.

    3D structure databases

    ProteinModelPortali Q9SCV4.
    SMRi Q9SCV4. Positions 31-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G28470.1-P.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Proteomic databases

    PaxDbi Q9SCV4.
    PRIDEi Q9SCV4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G28470.1 ; AT2G28470.1 ; AT2G28470 . [Q9SCV4-1 ]
    GeneIDi 817395.
    KEGGi ath:AT2G28470.

    Organism-specific databases

    GeneFarmi 508. 90.
    TAIRi AT2G28470.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000239919.
    InParanoidi Q9SCV4.
    OMAi NTAQINS.
    PhylomeDBi Q9SCV4.

    Enzyme and pathway databases

    BioCyci ARA:AT2G28470-MONOMER.
    ARA:GQT-1273-MONOMER.

    Gene expression databases

    ArrayExpressi Q9SCV4.
    Genevestigatori Q9SCV4.

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    IPR000922. Lectin_gal-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF02140. Gal_Lectin. 1 hit.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF49785. SSF49785. 3 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
      Gy I., Kreis M., Lecharny A.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant."
      Hirayama T., Ishida C., Kuromori T., Obata S., Shimoda C., Yamamoto M., Shinozaki K., Ohto C.
      FEBS Lett. 413:16-20(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 647-852.
    5. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
      Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
      Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
      Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
      Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGAL8_ARATH
    AccessioniPrimary (citable) accession number: Q9SCV4
    Secondary accession number(s): Q96257, Q9SK11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3