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Protein

Beta-galactosidase 8

Gene

BGAL8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Proton donorSequence Analysis
Active sitei257 – 2571NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: GO_Central
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G28470-MONOMER.
ARA:GQT-1273-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 8 (EC:3.2.1.23)
Short name:
Lactase 8
Alternative name(s):
Protein AR782
Gene namesi
Name:BGAL8
Ordered Locus Names:At2g28470
ORF Names:T17D12.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G28470.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: TAIR
  3. vacuole Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 852823Beta-galactosidase 8PRO_5000065882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi766 – 7661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi807 – 8071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV4.
PRIDEiQ9SCV4.

Expressioni

Tissue specificityi

Expressed in roots, flowers and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ9SCV4. baseline and differential.
GenevestigatoriQ9SCV4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G28470.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SCV4.
SMRiQ9SCV4. Positions 31-735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini766 – 85287SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV4.
OMAiKMEMILL.
PhylomeDBiQ9SCV4.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SCV4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIAAKMVKV RKMEMILLLI LVIVVAATAA NVTYDHRALV IDGKRKVLIS
60 70 80 90 100
GSIHYPRSTP EMWPELIQKS KDGGLDVIET YVFWSGHEPE KNKYNFEGRY
110 120 130 140 150
DLVKFVKLAA KAGLYVHLRI GPYVCAEWNY GGFPVWLHFV PGIKFRTDNE
160 170 180 190 200
PFKEEMQRFT TKIVDLMKQE KLYASQGGPI ILSQIENEYG NIDSAYGAAA
210 220 230 240 250
KSYIKWSASM ALSLDTGVPW NMCQQTDAPD PMINTCNGFY CDQFTPNSNN
260 270 280 290 300
KPKMWTENWS GWFLGFGDPS PYRPVEDLAF AVARFYQRGG TFQNYYMYHG
310 320 330 340 350
GTNFDRTSGG PLISTSYDYD APIDEYGLLR QPKWGHLRDL HKAIKLCEDA
360 370 380 390 400
LIATDPTITS LGSNLEAAVY KTESGSCAAF LANVDTKSDA TVTFNGKSYN
410 420 430 440 450
LPAWSVSILP DCKNVAFNTA KINSATESTA FARQSLKPDG GSSAELGSQW
460 470 480 490 500
SYIKEPIGIS KADAFLKPGL LEQINTTADK SDYLWYSLRT DIKGDETFLD
510 520 530 540 550
EGSKAVLHIE SLGQVVYAFI NGKLAGSGHG KQKISLDIPI NLVTGTNTID
560 570 580 590 600
LLSVTVGLAN YGAFFDLVGA GITGPVTLKS AKGGSSIDLA SQQWTYQVGL
610 620 630 640 650
KGEDTGLATV DSSEWVSKSP LPTKQPLIWY KTTFDAPSGS EPVAIDFTGT
660 670 680 690 700
GKGIAWVNGQ SIGRYWPTSI AGNGGCTESC DYRGSYRANK CLKNCGKPSQ
710 720 730 740 750
TLYHVPRSWL KPSGNILVLF EEMGGDPTQI SFATKQTGSN LCLTVSQSHP
760 770 780 790 800
PPVDTWTSDS KISNRNRTRP VLSLKCPIST QVIFSIKFAS FGTPKGTCGS
810 820 830 840 850
FTQGHCNSSR SLSLVQKACI GLRSCNVEVS TRVFGEPCRG VVKSLAVEAS

CS
Length:852
Mass (Da):93,210
Last modified:July 10, 2007 - v2
Checksum:i54DCFB09790EE045
GO

Sequence cautioni

The sequence AAD21482.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti568 – 5681V → M in CAB64744 (Ref. 1) Curated
Sequence conflicti647 – 6482FT → TA in BAA13685 (PubMed:9287109).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270304 mRNA. Translation: CAB64744.1.
AC006587 Genomic DNA. Translation: AAD21482.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08127.1.
D88744 mRNA. Translation: BAA13685.1.
PIRiC84685.
RefSeqiNP_850121.1. NM_179790.2. [Q9SCV4-1]
UniGeneiAt.20550.
At.66398.

Genome annotation databases

EnsemblPlantsiAT2G28470.1; AT2G28470.1; AT2G28470. [Q9SCV4-1]
GeneIDi817395.
KEGGiath:AT2G28470.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270304 mRNA. Translation: CAB64744.1.
AC006587 Genomic DNA. Translation: AAD21482.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08127.1.
D88744 mRNA. Translation: BAA13685.1.
PIRiC84685.
RefSeqiNP_850121.1. NM_179790.2. [Q9SCV4-1]
UniGeneiAt.20550.
At.66398.

3D structure databases

ProteinModelPortaliQ9SCV4.
SMRiQ9SCV4. Positions 31-735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G28470.1-P.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ9SCV4.
PRIDEiQ9SCV4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G28470.1; AT2G28470.1; AT2G28470. [Q9SCV4-1]
GeneIDi817395.
KEGGiath:AT2G28470.

Organism-specific databases

GeneFarmi508. 90.
TAIRiAT2G28470.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV4.
OMAiKMEMILL.
PhylomeDBiQ9SCV4.

Enzyme and pathway databases

BioCyciARA:AT2G28470-MONOMER.
ARA:GQT-1273-MONOMER.

Gene expression databases

ExpressionAtlasiQ9SCV4. baseline and differential.
GenevestigatoriQ9SCV4.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant."
    Hirayama T., Ishida C., Kuromori T., Obata S., Shimoda C., Yamamoto M., Shinozaki K., Ohto C.
    FEBS Lett. 413:16-20(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 647-852.
  5. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGAL8_ARATH
AccessioniPrimary (citable) accession number: Q9SCV4
Secondary accession number(s): Q96257, Q9SK11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: February 4, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.