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Q9SCV4 (BGAL8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 8

Short name=Lactase 8
EC=3.2.1.23
Alternative name(s):
Protein AR782
Gene names
Name:BGAL8
Ordered Locus Names:At2g28470
ORF Names:T17D12.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length852 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Expressed in roots, flowers and siliques. Ref.5 Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence AAD21482.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from direct assay PubMed 15593128. Source: TAIR

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SCV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 852823Beta-galactosidase 8
PRO_5000065882

Regions

Domain766 – 85287SUEL-type lectin

Sites

Active site1881Proton donor Potential
Active site2571Nucleophile Potential

Amino acid modifications

Glycosylation311N-linked (GlcNAc...) Potential
Glycosylation2581N-linked (GlcNAc...) Potential
Glycosylation4751N-linked (GlcNAc...) Potential
Glycosylation7661N-linked (GlcNAc...) Potential
Glycosylation8071N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5681V → M in CAB64744. Ref.1
Sequence conflict647 – 6482FT → TA in BAA13685. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 54DCFB09790EE045

FASTA85293,210
        10         20         30         40         50         60 
MEIAAKMVKV RKMEMILLLI LVIVVAATAA NVTYDHRALV IDGKRKVLIS GSIHYPRSTP 

        70         80         90        100        110        120 
EMWPELIQKS KDGGLDVIET YVFWSGHEPE KNKYNFEGRY DLVKFVKLAA KAGLYVHLRI 

       130        140        150        160        170        180 
GPYVCAEWNY GGFPVWLHFV PGIKFRTDNE PFKEEMQRFT TKIVDLMKQE KLYASQGGPI 

       190        200        210        220        230        240 
ILSQIENEYG NIDSAYGAAA KSYIKWSASM ALSLDTGVPW NMCQQTDAPD PMINTCNGFY 

       250        260        270        280        290        300 
CDQFTPNSNN KPKMWTENWS GWFLGFGDPS PYRPVEDLAF AVARFYQRGG TFQNYYMYHG 

       310        320        330        340        350        360 
GTNFDRTSGG PLISTSYDYD APIDEYGLLR QPKWGHLRDL HKAIKLCEDA LIATDPTITS 

       370        380        390        400        410        420 
LGSNLEAAVY KTESGSCAAF LANVDTKSDA TVTFNGKSYN LPAWSVSILP DCKNVAFNTA 

       430        440        450        460        470        480 
KINSATESTA FARQSLKPDG GSSAELGSQW SYIKEPIGIS KADAFLKPGL LEQINTTADK 

       490        500        510        520        530        540 
SDYLWYSLRT DIKGDETFLD EGSKAVLHIE SLGQVVYAFI NGKLAGSGHG KQKISLDIPI 

       550        560        570        580        590        600 
NLVTGTNTID LLSVTVGLAN YGAFFDLVGA GITGPVTLKS AKGGSSIDLA SQQWTYQVGL 

       610        620        630        640        650        660 
KGEDTGLATV DSSEWVSKSP LPTKQPLIWY KTTFDAPSGS EPVAIDFTGT GKGIAWVNGQ 

       670        680        690        700        710        720 
SIGRYWPTSI AGNGGCTESC DYRGSYRANK CLKNCGKPSQ TLYHVPRSWL KPSGNILVLF 

       730        740        750        760        770        780 
EEMGGDPTQI SFATKQTGSN LCLTVSQSHP PPVDTWTSDS KISNRNRTRP VLSLKCPIST 

       790        800        810        820        830        840 
QVIFSIKFAS FGTPKGTCGS FTQGHCNSSR SLSLVQKACI GLRSCNVEVS TRVFGEPCRG 

       850 
VVKSLAVEAS CS 

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant."
Hirayama T., Ishida C., Kuromori T., Obata S., Shimoda C., Yamamoto M., Shinozaki K., Ohto C.
FEBS Lett. 413:16-20(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 647-852.
[5]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270304 mRNA. Translation: CAB64744.1.
AC006587 Genomic DNA. Translation: AAD21482.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08127.1.
D88744 mRNA. Translation: BAA13685.1.
PIRC84685.
RefSeqNP_850121.1. NM_179790.2.
UniGeneAt.20550.
At.66398.

3D structure databases

ProteinModelPortalQ9SCV4.
SMRQ9SCV4. Positions 28-733, 771-852.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G28470.1-P.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9SCV4.
PRIDEQ9SCV4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G28470.1; AT2G28470.1; AT2G28470. [Q9SCV4-1]
GeneID817395.
KEGGath:AT2G28470.

Organism-specific databases

GeneFarm508. 90.
TAIRAT2G28470.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9SCV4.
OMAFYQRGGT.
PhylomeDBQ9SCV4.
ProtClustDBCLSN2690970.

Enzyme and pathway databases

BioCycARA:AT2G28470-MONOMER.
ARA:GQT-1273-MONOMER.

Gene expression databases

ArrayExpressQ9SCV4.
GenevestigatorQ9SCV4.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL8_ARATH
AccessionPrimary (citable) accession number: Q9SCV4
Secondary accession number(s): Q96257, Q9SK11
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names