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Q9SCV4

- BGAL8_ARATH

UniProt

Q9SCV4 - BGAL8_ARATH

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Protein
Beta-galactosidase 8
Gene
BGAL8, At2g28470, T17D12.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Proton donor Reviewed prediction
Active sitei257 – 2571Nucleophile Reviewed prediction

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G28470-MONOMER.
ARA:GQT-1273-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 8 (EC:3.2.1.23)
Short name:
Lactase 8
Alternative name(s):
Protein AR782
Gene namesi
Name:BGAL8
Ordered Locus Names:At2g28470
ORF Names:T17D12.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G28470.

Subcellular locationi

Secretedextracellular spaceapoplast Reviewed prediction

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 852823Beta-galactosidase 8
PRO_5000065882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi31 – 311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi258 – 2581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi475 – 4751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi766 – 7661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi807 – 8071N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV4.
PRIDEiQ9SCV4.

Expressioni

Tissue specificityi

Expressed in roots, flowers and siliques.2 Publications

Gene expression databases

ArrayExpressiQ9SCV4.
GenevestigatoriQ9SCV4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G28470.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SCV4.
SMRiQ9SCV4. Positions 31-735.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini766 – 85287SUEL-type lectin
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV4.
OMAiNTAQINS.
PhylomeDBiQ9SCV4.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SCV4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEIAAKMVKV RKMEMILLLI LVIVVAATAA NVTYDHRALV IDGKRKVLIS    50
GSIHYPRSTP EMWPELIQKS KDGGLDVIET YVFWSGHEPE KNKYNFEGRY 100
DLVKFVKLAA KAGLYVHLRI GPYVCAEWNY GGFPVWLHFV PGIKFRTDNE 150
PFKEEMQRFT TKIVDLMKQE KLYASQGGPI ILSQIENEYG NIDSAYGAAA 200
KSYIKWSASM ALSLDTGVPW NMCQQTDAPD PMINTCNGFY CDQFTPNSNN 250
KPKMWTENWS GWFLGFGDPS PYRPVEDLAF AVARFYQRGG TFQNYYMYHG 300
GTNFDRTSGG PLISTSYDYD APIDEYGLLR QPKWGHLRDL HKAIKLCEDA 350
LIATDPTITS LGSNLEAAVY KTESGSCAAF LANVDTKSDA TVTFNGKSYN 400
LPAWSVSILP DCKNVAFNTA KINSATESTA FARQSLKPDG GSSAELGSQW 450
SYIKEPIGIS KADAFLKPGL LEQINTTADK SDYLWYSLRT DIKGDETFLD 500
EGSKAVLHIE SLGQVVYAFI NGKLAGSGHG KQKISLDIPI NLVTGTNTID 550
LLSVTVGLAN YGAFFDLVGA GITGPVTLKS AKGGSSIDLA SQQWTYQVGL 600
KGEDTGLATV DSSEWVSKSP LPTKQPLIWY KTTFDAPSGS EPVAIDFTGT 650
GKGIAWVNGQ SIGRYWPTSI AGNGGCTESC DYRGSYRANK CLKNCGKPSQ 700
TLYHVPRSWL KPSGNILVLF EEMGGDPTQI SFATKQTGSN LCLTVSQSHP 750
PPVDTWTSDS KISNRNRTRP VLSLKCPIST QVIFSIKFAS FGTPKGTCGS 800
FTQGHCNSSR SLSLVQKACI GLRSCNVEVS TRVFGEPCRG VVKSLAVEAS 850
CS 852
Length:852
Mass (Da):93,210
Last modified:July 10, 2007 - v2
Checksum:i54DCFB09790EE045
GO

Sequence cautioni

The sequence AAD21482.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti568 – 5681V → M in CAB64744. 1 Publication
Sequence conflicti647 – 6482FT → TA in BAA13685. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ270304 mRNA. Translation: CAB64744.1.
AC006587 Genomic DNA. Translation: AAD21482.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08127.1.
D88744 mRNA. Translation: BAA13685.1.
PIRiC84685.
RefSeqiNP_850121.1. NM_179790.2. [Q9SCV4-1]
UniGeneiAt.20550.
At.66398.

Genome annotation databases

EnsemblPlantsiAT2G28470.1; AT2G28470.1; AT2G28470. [Q9SCV4-1]
GeneIDi817395.
KEGGiath:AT2G28470.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ270304 mRNA. Translation: CAB64744.1 .
AC006587 Genomic DNA. Translation: AAD21482.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC08127.1 .
D88744 mRNA. Translation: BAA13685.1 .
PIRi C84685.
RefSeqi NP_850121.1. NM_179790.2. [Q9SCV4-1 ]
UniGenei At.20550.
At.66398.

3D structure databases

ProteinModelPortali Q9SCV4.
SMRi Q9SCV4. Positions 31-735.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G28470.1-P.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbi Q9SCV4.
PRIDEi Q9SCV4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G28470.1 ; AT2G28470.1 ; AT2G28470 . [Q9SCV4-1 ]
GeneIDi 817395.
KEGGi ath:AT2G28470.

Organism-specific databases

GeneFarmi 508. 90.
TAIRi AT2G28470.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000239919.
InParanoidi Q9SCV4.
OMAi NTAQINS.
PhylomeDBi Q9SCV4.

Enzyme and pathway databases

BioCyci ARA:AT2G28470-MONOMER.
ARA:GQT-1273-MONOMER.

Gene expression databases

ArrayExpressi Q9SCV4.
Genevestigatori Q9SCV4.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutant."
    Hirayama T., Ishida C., Kuromori T., Obata S., Shimoda C., Yamamoto M., Shinozaki K., Ohto C.
    FEBS Lett. 413:16-20(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 647-852.
  5. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGAL8_ARATH
AccessioniPrimary (citable) accession number: Q9SCV4
Secondary accession number(s): Q96257, Q9SK11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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