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Protein

Beta-galactosidase 9

Gene

BGAL9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei194 – 1941Proton donorSequence Analysis
Active sitei263 – 2631NucleophileSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G32810-MONOMER.
ARA:GQT-2715-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 9 (EC:3.2.1.23)
Short name:
Lactase 9
Gene namesi
Name:BGAL9
Ordered Locus Names:At2g32810
ORF Names:F24L7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G32810.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • plant-type cell wall Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 887857Beta-galactosidase 9PRO_5000065883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi463 – 4631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi485 – 4851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi496 – 4961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi527 – 5271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi785 – 7851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi881 – 8811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV3.
PRIDEiQ9SCV3.

Expressioni

Tissue specificityi

Ubiquitous, with higher expression levels in siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ9SCV3. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G32810.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SCV3.
SMRiQ9SCV3. Positions 36-755.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini791 – 87787SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV3.
OMAiGSKQEAH.
PhylomeDBiQ9SCV3.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SCV3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESIRTFSL QWRILSLIIA LLVYFPILSG SYFKPFNVSY DHRALIIAGK
60 70 80 90 100
RRMLVSAGIH YPRATPEMWS DLIAKSKEGG ADVVQTYVFW NGHEPVKGQY
110 120 130 140 150
NFEGRYDLVK FVKLIGSSGL YLHLRIGPYV CAEWNFGGFP VWLRDIPGIE
160 170 180 190 200
FRTDNEPFKK EMQKFVTKIV DLMREAKLFC WQGGPIIMLQ IENEYGDVEK
210 220 230 240 250
SYGQKGKDYV KWAASMALGL GAGVPWVMCK QTDAPENIID ACNGYYCDGF
260 270 280 290 300
KPNSRTKPVL WTEDWDGWYT KWGGSLPHRP AEDLAFAVAR FYQRGGSFQN
310 320 330 340 350
YYMYFGGTNF GRTSGGPFYI TSYDYDAPLD EYGLRSEPKW GHLKDLHAAI
360 370 380 390 400
KLCEPALVAA DAPQYRKLGS KQEAHIYHGD GETGGKVCAA FLANIDEHKS
410 420 430 440 450
AHVKFNGQSY TLPPWSVSIL PDCRHVAFNT AKVGAQTSVK TVESARPSLG
460 470 480 490 500
SMSILQKVVR QDNVSYISKS WMALKEPIGI WGENNFTFQG LLEHLNVTKD
510 520 530 540 550
RSDYLWHKTR ISVSEDDISF WKKNGPNSTV SIDSMRDVLR VFVNKQLAGS
560 570 580 590 600
IVGHWVKAVQ PVRFIQGNND LLLLTQTVGL QNYGAFLEKD GAGFRGKAKL
610 620 630 640 650
TGFKNGDLDL SKSSWTYQVG LKGEADKIYT VEHNEKAEWS TLETDASPSI
660 670 680 690 700
FMWYKTYFDP PAGTDPVVLN LESMGRGQAW VNGQHIGRYW NIISQKDGCD
710 720 730 740 750
RTCDYRGAYN SDKCTTNCGK PTQTRYHVPR SWLKPSSNLL VLFEETGGNP
760 770 780 790 800
FKISVKTVTA GILCGQVSES HYPPLRKWST PDYINGTMSI NSVAPEVHLH
810 820 830 840 850
CEDGHVISSI EFASYGTPRG SCDGFSIGKC HASNSLSIVS EACKGRNSCF
860 870 880
IEVSNTAFIS DPCSGTLKTL AVMSRCSPSQ NMSDLSF
Length:887
Mass (Da):99,199
Last modified:May 1, 2000 - v1
Checksum:i51FC80516B1C85EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270305 mRNA. Translation: CAB64745.1.
AC003974 Genomic DNA. Translation: AAC04500.2.
CP002685 Genomic DNA. Translation: AEC08744.1.
PIRiT00787.
RefSeqiNP_565755.1. NM_128841.4. [Q9SCV3-1]
UniGeneiAt.66358.
At.704.

Genome annotation databases

EnsemblPlantsiAT2G32810.1; AT2G32810.1; AT2G32810. [Q9SCV3-1]
GeneIDi817842.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270305 mRNA. Translation: CAB64745.1.
AC003974 Genomic DNA. Translation: AAC04500.2.
CP002685 Genomic DNA. Translation: AEC08744.1.
PIRiT00787.
RefSeqiNP_565755.1. NM_128841.4. [Q9SCV3-1]
UniGeneiAt.66358.
At.704.

3D structure databases

ProteinModelPortaliQ9SCV3.
SMRiQ9SCV3. Positions 36-755.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G32810.1.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ9SCV3.
PRIDEiQ9SCV3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32810.1; AT2G32810.1; AT2G32810. [Q9SCV3-1]
GeneIDi817842.

Organism-specific databases

GeneFarmi510. 90.
TAIRiAT2G32810.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV3.
OMAiGSKQEAH.
PhylomeDBiQ9SCV3.

Enzyme and pathway databases

BioCyciARA:AT2G32810-MONOMER.
ARA:GQT-2715-MONOMER.

Miscellaneous databases

PROiQ9SCV3.

Gene expression databases

ExpressionAtlasiQ9SCV3. baseline and differential.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGAL9_ARATH
AccessioniPrimary (citable) accession number: Q9SCV3
Secondary accession number(s): O48836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.