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Q9SCV3 (BGAL9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 9
Short name=Lactase 9
EC=3.2.1.23
Gene names
Name:BGAL9
Ordered Locus Names:At2g32810
ORF Names:F24L7.5
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secreted › extracellular space › apoplast Potential.

Tissue specificity

Ubiquitous, with higher expression levels in siliques. Ref.4 Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

plant-type cell wall

Inferred from direct assay. Source: TAIR

vacuolar membrane

Inferred from direct assay. Source: TAIR

   Molecular functionbeta-galactosidase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

sugar binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SCV3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 887857Beta-galactosidase 9
PRO_5000065883

Regions

Domain791 – 87787SUEL-type lectin

Sites

Active site1941Proton donor Potential
Active site2631Nucleophile Potential

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation4631N-linked (GlcNAc...) Potential
Glycosylation4851N-linked (GlcNAc...) Potential
Glycosylation4961N-linked (GlcNAc...) Potential
Glycosylation5271N-linked (GlcNAc...) Potential
Glycosylation7851N-linked (GlcNAc...) Potential
Glycosylation8811N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 51FC80516B1C85EC

FASTA88799,199
        10         20         30         40         50         60 
MAESIRTFSL QWRILSLIIA LLVYFPILSG SYFKPFNVSY DHRALIIAGK RRMLVSAGIH 

        70         80         90        100        110        120 
YPRATPEMWS DLIAKSKEGG ADVVQTYVFW NGHEPVKGQY NFEGRYDLVK FVKLIGSSGL 

       130        140        150        160        170        180 
YLHLRIGPYV CAEWNFGGFP VWLRDIPGIE FRTDNEPFKK EMQKFVTKIV DLMREAKLFC 

       190        200        210        220        230        240 
WQGGPIIMLQ IENEYGDVEK SYGQKGKDYV KWAASMALGL GAGVPWVMCK QTDAPENIID 

       250        260        270        280        290        300 
ACNGYYCDGF KPNSRTKPVL WTEDWDGWYT KWGGSLPHRP AEDLAFAVAR FYQRGGSFQN 

       310        320        330        340        350        360 
YYMYFGGTNF GRTSGGPFYI TSYDYDAPLD EYGLRSEPKW GHLKDLHAAI KLCEPALVAA 

       370        380        390        400        410        420 
DAPQYRKLGS KQEAHIYHGD GETGGKVCAA FLANIDEHKS AHVKFNGQSY TLPPWSVSIL 

       430        440        450        460        470        480 
PDCRHVAFNT AKVGAQTSVK TVESARPSLG SMSILQKVVR QDNVSYISKS WMALKEPIGI 

       490        500        510        520        530        540 
WGENNFTFQG LLEHLNVTKD RSDYLWHKTR ISVSEDDISF WKKNGPNSTV SIDSMRDVLR 

       550        560        570        580        590        600 
VFVNKQLAGS IVGHWVKAVQ PVRFIQGNND LLLLTQTVGL QNYGAFLEKD GAGFRGKAKL 

       610        620        630        640        650        660 
TGFKNGDLDL SKSSWTYQVG LKGEADKIYT VEHNEKAEWS TLETDASPSI FMWYKTYFDP 

       670        680        690        700        710        720 
PAGTDPVVLN LESMGRGQAW VNGQHIGRYW NIISQKDGCD RTCDYRGAYN SDKCTTNCGK 

       730        740        750        760        770        780 
PTQTRYHVPR SWLKPSSNLL VLFEETGGNP FKISVKTVTA GILCGQVSES HYPPLRKWST 

       790        800        810        820        830        840 
PDYINGTMSI NSVAPEVHLH CEDGHVISSI EFASYGTPRG SCDGFSIGKC HASNSLSIVS 

       850        860        870        880 
EACKGRNSCF IEVSNTAFIS DPCSGTLKTL AVMSRCSPSQ NMSDLSF

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed: 16267099] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ270305 mRNA. Translation: CAB64745.1.
AC003974 Genomic DNA. Translation: AAC04500.2.
CP002685 Genomic DNA. Translation: AEC08744.1.
IPIIPI00530466.
PIRT00787.
RefSeqNP_565755.1. NM_128841.4.
UniGeneAt.66358.
At.704.

3D structure databases

ProteinModelPortalQ9SCV3.
SMRQ9SCV3. Positions 65-151, 796-880.
ModBaseSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEQ9SCV3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32810.1; AT2G32810.1; AT2G32810.
GeneID817842.
GenomeReviewsGene locus AT2G32810 in contig CT485783_GR.
KEGGath:AT2G32810.
NMPDRfig|3702.1.peg.10360.

Organism-specific databases

GeneFarm510. 90.
TAIRAt2g32810.

Phylogenomic databases

eggNOGKOG0496.
GeneTreeEPGT00070000029152.
HOGENOMHBG318019.
InParanoidQ9SCV3.
OMAKDGAGFR.
PhylomeDBQ9SCV3.
ProtClustDBCLSN2688643.

Gene expression databases

ArrayExpressQ9SCV3.
GenevestigatorQ9SCV3.

Family and domain databases

InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR23421. Glyco_hydro_35. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. Gal_bind_like. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL9_ARATH
AccessionPrimary (citable) accession number: Q9SCV3
Secondary accession number(s): O48836
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: September 21, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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