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Q9SCV0

- BGA12_ARATH

UniProt

Q9SCV0 - BGA12_ARATH

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Protein
Beta-galactosidase 12
Gene
BGAL12, At4g26140, F20B18.250
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei185 – 1851Proton donor Reviewed prediction
Active sitei254 – 2541Nucleophile Reviewed prediction

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT4G26140-MONOMER.
ARA:GQT-2664-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 12 (EC:3.2.1.23)
Short name:
Lactase 12
Gene namesi
Name:BGAL12
Ordered Locus Names:At4g26140
ORF Names:F20B18.250
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G26140.

Subcellular locationi

Secretedextracellular spaceapoplast Reviewed prediction

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Chaini28 – 728701Beta-galactosidase 12
PRO_5000065885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi255 – 2551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi380 – 3801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi450 – 4501N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCV0.
PRIDEiQ9SCV0.

Expressioni

Tissue specificityi

Ubiquitous, with higher expression levels in roots and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9SCV0.

Structurei

3D structure databases

ProteinModelPortaliQ9SCV0.
SMRiQ9SCV0. Positions 29-726.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCV0.
OMAiNSIINTC.
PhylomeDBiQ9SCV0.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02837. Glyco_hydro_2_N. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9SCV0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGLNFREKAW ILLGILCCSS LICSVKAIVT YDRKAVIING QRRILLSGSI    50
HYPRSTPEMW PDLIQKAKDG GLDVIQTYVF WNGHEPSPGQ YYFEDRYDLV 100
KFIKVVQQAG LYVHLRIGPY VCAEWNFGGF PVWLKYVPGM VFRTDNEPFK 150
AAMQKFTEKI VRMMKEEKLF ETQGGPIILS QIENEYGPIE WEIGAPGKAY 200
TKWVAEMAQG LSTGVPWIMC KQDDAPNSII NTCNGFYCEN FKPNSDNKPK 250
MWTENWTGWF TEFGGAVPYR PAEDIALSVA RFIQNGGSFI NYYMYHGGTN 300
FDRTAGEFIA TSYDYDAPLD EYGLPREPKY SHLKRLHKVI KLCEPALVSA 350
DPTVTSLGDK QEAHVFKSKS SCAAFLSNYN TSSAARVLFG GSTYDLPPWS 400
VSILPDCKTE YYNTAKVQVR TSSIHMKMVP TNTPFSWGSY NEEIPSANDN 450
GTFSQDGLVE QISITRDKTD YFWYLTDITI SPDEKFLTGE DPLLTIGSAG 500
HALHVFVNGQ LAGTAYGSLE KPKLTFSQKI KLHAGVNKLA LLSTAAGLPN 550
VGVHYETWNT GVLGPVTLNG VNSGTWDMTK WKWSYKIGTK GEALSVHTLA 600
GSSTVEWKEG SLVAKKQPLT WYKSTFDSPT GNEPLALDMN TMGKGQMWIN 650
GQNIGRHWPA YTARGKCERC SYAGTFTEKK CLSNCGEASQ RWYHVPRSWL 700
KPTNNLVIVL EEWGGEPNGI SLVKRTAK 728
Length:728
Mass (Da):81,533
Last modified:May 1, 2000 - v1
Checksum:i75EB099914A7BA65
GO
Isoform 2 (identifier: Q9SCV0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     418-419: Missing.
     624-638: STFDSPTGNEPLALD → VRETEESMNHDHQQS
     639-728: Missing.

Note: May be due to a competing acceptor splice site and to an intron retention. No experimental confirmation available.

Show »
Length:636
Mass (Da):71,357
Checksum:i11F0DC8FDB69283D
GO

Sequence cautioni

The sequence CAB39679.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB79469.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei418 – 4192Missing in isoform 2.
VSP_026465
Alternative sequencei624 – 63815STFDS…PLALD → VRETEESMNHDHQQS in isoform 2.
VSP_026466Add
BLAST
Alternative sequencei639 – 72890Missing in isoform 2.
VSP_026467Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ270308 mRNA. Translation: CAB64748.1.
AL049483 Genomic DNA. Translation: CAB39679.1. Sequence problems.
AL161564 Genomic DNA. Translation: CAB79469.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE85162.1.
CP002687 Genomic DNA. Translation: AEE85163.1.
AY099612 mRNA. Translation: AAM20463.1.
BT000267 mRNA. Translation: AAN15586.1.
PIRiT04269.
RefSeqiNP_194344.2. NM_118747.2. [Q9SCV0-2]
NP_849553.1. NM_179222.1. [Q9SCV0-1]
UniGeneiAt.2962.

Genome annotation databases

EnsemblPlantsiAT4G26140.1; AT4G26140.1; AT4G26140. [Q9SCV0-1]
GeneIDi828720.
KEGGiath:AT4G26140.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ270308 mRNA. Translation: CAB64748.1 .
AL049483 Genomic DNA. Translation: CAB39679.1 . Sequence problems.
AL161564 Genomic DNA. Translation: CAB79469.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE85162.1 .
CP002687 Genomic DNA. Translation: AEE85163.1 .
AY099612 mRNA. Translation: AAM20463.1 .
BT000267 mRNA. Translation: AAN15586.1 .
PIRi T04269.
RefSeqi NP_194344.2. NM_118747.2. [Q9SCV0-2 ]
NP_849553.1. NM_179222.1. [Q9SCV0-1 ]
UniGenei At.2962.

3D structure databases

ProteinModelPortali Q9SCV0.
SMRi Q9SCV0. Positions 29-726.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbi Q9SCV0.
PRIDEi Q9SCV0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G26140.1 ; AT4G26140.1 ; AT4G26140 . [Q9SCV0-1 ]
GeneIDi 828720.
KEGGi ath:AT4G26140.

Organism-specific databases

GeneFarmi 514. 90.
TAIRi AT4G26140.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000239919.
InParanoidi Q9SCV0.
OMAi NSIINTC.
PhylomeDBi Q9SCV0.

Enzyme and pathway databases

BioCyci ARA:AT4G26140-MONOMER.
ARA:GQT-2664-MONOMER.

Gene expression databases

Genevestigatori Q9SCV0.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF02837. Glyco_hydro_2_N. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
    Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
    Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGA12_ARATH
AccessioniPrimary (citable) accession number: Q9SCV0
Secondary accession number(s): Q8LPL0, Q9SZI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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