Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9SCU9 (BGA13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 13

Short name=Lactase 13
EC=3.2.1.23
Gene names
Name:BGAL13
Ordered Locus Names:At2g16730
ORF Names:T24I21.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Ubiquitous, with higher expression levels in roots, flowers and siliques. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Contains 1 SUEL-type lectin domain.

Sequence caution

The sequence AAD24606.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Traceable author statement PubMed 15517348. Source: TAIR

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 848821Beta-galactosidase 13
PRO_5000065886

Regions

Domain754 – 84390SUEL-type lectin

Sites

Active site2001Proton donor Potential
Active site2711Nucleophile Potential

Amino acid modifications

Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3761N-linked (GlcNAc...) Potential
Glycosylation3981N-linked (GlcNAc...) Potential
Glycosylation7821N-linked (GlcNAc...) Potential
Glycosylation7871N-linked (GlcNAc...) Potential
Glycosylation8171N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9SCU9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 1AF1AF48C1FA5F1B

FASTA84895,900
        10         20         30         40         50         60 
MKIHSSDHSW LLLAVLVILL SFSGALSSDD KEKKTKSVDK KKEVTYDGTS LIINGNRELL 

        70         80         90        100        110        120 
YSGSIHYPRS TPEMWPNIIK RAKQGGLNTI QTYVFWNVHE PEQGKFNFSG RADLVKFIKL 

       130        140        150        160        170        180 
IEKNGLYVTL RLGPFIQAEW THGGLPYWLR EVPGIFFRTD NEPFKEHTER YVKVVLDMMK 

       190        200        210        220        230        240 
EEKLFASQGG PIILGQIENE YSAVQRAYKE DGLNYIKWAS KLVHSMDLGI PWVMCKQNDA 

       250        260        270        280        290        300 
PDPMINACNG RHCGDTFPGP NKDNKPSLWT ENWTTQFRVF GDPPAQRSVE DIAYSVARFF 

       310        320        330        340        350        360 
SKNGTHVNYY MYHGGTNFGR TSAHYVTTRY YDDAPLDEFG LEREPKYGHL KHLHNALNLC 

       370        380        390        400        410        420 
KKALLWGQPR VEKPSNETEI RYYEQPGTKV CAAFLANNNT EAAEKIKFRG KEYLIPHRSI 

       430        440        450        460        470        480 
SILPDCKTVV YNTGEIISHH TSRNFMKSKK ANKNFDFKVF TESVPSKIKG DSFIPVELYG 

       490        500        510        520        530        540 
LTKDESDYGW YTTSFKIDDN DLSKKKGGKP NLRIASLGHA LHVWLNGEYL GNGHGSHEEK 

       550        560        570        580        590        600 
SFVFQKPVTL KEGENHLTML GVLTGFPDSG SYMEHRYTGP RSVSILGLGS GTLDLTEENK 

       610        620        630        640        650        660 
WGNKVGMEGE RLGIHAEEGL KKVKWEKASG KEPGMTWYQT YFDAPESQSA AAIRMNGMGK 

       670        680        690        700        710        720 
GLIWVNGEGV GRYWMSFLSP LGQPTQIEYH IPRSFLKPKK NLLVIFEEEP NVKPELIDFV 

       730        740        750        760        770        780 
IVNRDTVCSY IGENYTPSVR HWTRKNDQVQ AITDDVHLTA NLKCSGTKKI SAVEFASFGN 

       790        800        810        820        830        840 
PNGTCGNFTL GSCNAPVSKK VVEKYCLGKA ECVIPVNKST FEQDKKDSCP KVEKKLAVQV 


KCGRDKKN 

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270309 mRNA. Translation: CAB64749.1.
AC005825 Genomic DNA. Translation: AAD24606.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06532.1.
PIRE84543.
RefSeqNP_179264.2. NM_127225.2.
UniGeneAt.707.

3D structure databases

ProteinModelPortalQ9SCU9.
SMRQ9SCU9. Positions 43-353, 759-847.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9SCU9.
PRIDEQ9SCU9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G16730.1; AT2G16730.1; AT2G16730.
GeneID816174.
KEGGath:AT2G16730.

Organism-specific databases

GeneFarm515. 90.
TAIRAT2G16730.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9SCU9.
OMANETEIRY.
PhylomeDBQ9SCU9.
ProtClustDBCLSN2689764.

Enzyme and pathway databases

BioCycARA:AT2G16730-MONOMER.

Gene expression databases

GenevestigatorQ9SCU9.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF02140. Gal_Lectin. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGA13_ARATH
AccessionPrimary (citable) accession number: Q9SCU9
Secondary accession number(s): Q9SLE5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 1, 2000
Last modified: March 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names