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Protein

Beta-galactosidase 14

Gene

BGAL14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197Proton donorSequence analysis1
Active sitei268NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 14 (EC:3.2.1.23)
Short name:
Lactase 14
Gene namesi
Name:BGAL14
Ordered Locus Names:At4g38590
ORF Names:F20M13.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G38590.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_500006588732 – 887Beta-galactosidase 14Add BLAST856

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1
Glycosylationi785N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCU8.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SCU8. baseline and differential.
GenevisibleiQ9SCU8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G38590.2.

Structurei

3D structure databases

ProteinModelPortaliQ9SCU8.
SMRiQ9SCU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini752 – 838SUEL-type lectinPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
KOG0496. Eukaryota.
COG1874. LUCA.
HOGENOMiHOG000239919.
InParanoidiQ9SCU8.
PhylomeDBiQ9SCU8.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9SCU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKSSRIRMK SRTRYLIAIL LVISLCSKAS SHDDEKKKKG VTYDGTSLII
60 70 80 90 100
NGKRELLFSG SVHYPRSTPH MWPSIIDKAR IGGLNTIQTY VFWNVHEPEQ
110 120 130 140 150
GKYDFKGRFD LVKFIKLIHE KGLYVTLRLG PFIQAEWNHG GLPYWLREVP
160 170 180 190 200
DVYFRTNNEP FKEHTERYVR KILGMMKEEK LFASQGGPII LGQIENEYNA
210 220 230 240 250
VQLAYKENGE KYIKWAANLV ESMNLGIPWV MCKQNDAPGN LINACNGRHC
260 270 280 290 300
GDTFPGPNRH DKPSLWTENW TTQFRVFGDP PTQRTVEDIA FSVARYFSKN
310 320 330 340 350
GSHVNYYMYH GGTNFGRTSA HFVTTRYYDD APLDEFGLEK APKYGHLKHV
360 370 380 390 400
HRALRLCKKA LFWGQLRAQT LGPDTEVRYY EQPGTKVCAA FLSNNNTRDT
410 420 430 440 450
NTIKFKGQDY VLPSRSISIL PDCKTVVYNT AQIVAQHSWR DFVKSEKTSK
460 470 480 490 500
GLKFEMFSEN IPSLLDGDSL IPGELYYLTK DKTDYAWYTT SVKIDEDDFP
510 520 530 540 550
DQKGLKTILR VASLGHALIV YVNGEYAGKA HGRHEMKSFE FAKPVNFKTG
560 570 580 590 600
DNRISILGVL TGLPDSGSYM EHRFAGPRAI SIIGLKSGTR DLTENNEWGH
610 620 630 640 650
LAGLEGEKKE VYTEEGSKKV KWEKDGKRKP LTWYKTYFET PEGVNAVAIR
660 670 680 690 700
MKAMGKGLIW VNGIGVGRYW MSFLSPLGEP TQTEYHIPRS FMKGEKKKNM
710 720 730 740 750
LVILEEEPGV KLESIDFVLV NRDTICSNVG EDYPVSVKSW KREGPKIVSR
760 770 780 790 800
SKDMRLKAVM RCPPEKQMVE VQFASFGDPT GTCGNFTMGK CSASKSKEVV
810 820 830 840 850
EKECLGRNYC SIVVARETFG DKGCPEIVKT LAVQVKCEKK EGKQDEKKKK
860 870 880
EDKDEEEEDD EDDDEEEEEE DKENKDTKDM ENKNQDM
Length:887
Mass (Da):101,222
Last modified:July 10, 2007 - v2
Checksum:i58486CB4B949508D
GO

Sequence cautioni

The sequence AEE86951 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80523 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57L → F in CAB64750 (Ref. 1) Curated1
Sequence conflicti70H → D in CAB64750 (Ref. 1) Curated1
Sequence conflicti286V → A in CAB64750 (Ref. 1) Curated1
Sequence conflicti627K → E in CAB64750 (Ref. 1) Curated1
Sequence conflicti653A → G in CAB64750 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270310 mRNA. Translation: CAB64750.1.
AL035540 Genomic DNA. Translation: CAB37515.1. Sequence problems.
AL161593 Genomic DNA. Translation: CAB80523.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86951.1. Sequence problems.
PIRiT05687.
RefSeqiNP_195571.3. NM_120020.4.
UniGeneiAt.70242.

Genome annotation databases

EnsemblPlantsiAT4G38590.1; AT4G38590.1; AT4G38590.
GeneIDi830016.
GrameneiAT4G38590.1; AT4G38590.1; AT4G38590.
KEGGiath:AT4G38590.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270310 mRNA. Translation: CAB64750.1.
AL035540 Genomic DNA. Translation: CAB37515.1. Sequence problems.
AL161593 Genomic DNA. Translation: CAB80523.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86951.1. Sequence problems.
PIRiT05687.
RefSeqiNP_195571.3. NM_120020.4.
UniGeneiAt.70242.

3D structure databases

ProteinModelPortaliQ9SCU8.
SMRiQ9SCU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G38590.2.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ9SCU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G38590.1; AT4G38590.1; AT4G38590.
GeneIDi830016.
GrameneiAT4G38590.1; AT4G38590.1; AT4G38590.
KEGGiath:AT4G38590.

Organism-specific databases

TAIRiAT4G38590.

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
KOG0496. Eukaryota.
COG1874. LUCA.
HOGENOMiHOG000239919.
InParanoidiQ9SCU8.
PhylomeDBiQ9SCU8.

Miscellaneous databases

PROiQ9SCU8.

Gene expression databases

ExpressionAtlasiQ9SCU8. baseline and differential.
GenevisibleiQ9SCU8. AT.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGA14_ARATH
AccessioniPrimary (citable) accession number: Q9SCU8
Secondary accession number(s): F4JUE4, Q9SZN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.