Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase 14

Gene

BGAL14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei197 – 1971Proton donorSequence Analysis
Active sitei268 – 2681NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:GQT-2802-MONOMER.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 14 (EC:3.2.1.23)
Short name:
Lactase 14
Gene namesi
Name:BGAL14
Ordered Locus Names:At4g38590
ORF Names:F20M13.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G38590.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 887856Beta-galactosidase 14PRO_5000065887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi785 – 7851N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SCU8.
PRIDEiQ9SCU8.

Expressioni

Gene expression databases

GenevestigatoriQ9SCU8.

Structurei

3D structure databases

ProteinModelPortaliQ9SCU8.
SMRiQ9SCU8. Positions 41-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini752 – 83887SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCU8.
PhylomeDBiQ9SCU8.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9SCU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKSSRIRMK SRTRYLIAIL LVISLCSKAS SHDDEKKKKG VTYDGTSLII
60 70 80 90 100
NGKRELLFSG SVHYPRSTPH MWPSIIDKAR IGGLNTIQTY VFWNVHEPEQ
110 120 130 140 150
GKYDFKGRFD LVKFIKLIHE KGLYVTLRLG PFIQAEWNHG GLPYWLREVP
160 170 180 190 200
DVYFRTNNEP FKEHTERYVR KILGMMKEEK LFASQGGPII LGQIENEYNA
210 220 230 240 250
VQLAYKENGE KYIKWAANLV ESMNLGIPWV MCKQNDAPGN LINACNGRHC
260 270 280 290 300
GDTFPGPNRH DKPSLWTENW TTQFRVFGDP PTQRTVEDIA FSVARYFSKN
310 320 330 340 350
GSHVNYYMYH GGTNFGRTSA HFVTTRYYDD APLDEFGLEK APKYGHLKHV
360 370 380 390 400
HRALRLCKKA LFWGQLRAQT LGPDTEVRYY EQPGTKVCAA FLSNNNTRDT
410 420 430 440 450
NTIKFKGQDY VLPSRSISIL PDCKTVVYNT AQIVAQHSWR DFVKSEKTSK
460 470 480 490 500
GLKFEMFSEN IPSLLDGDSL IPGELYYLTK DKTDYAWYTT SVKIDEDDFP
510 520 530 540 550
DQKGLKTILR VASLGHALIV YVNGEYAGKA HGRHEMKSFE FAKPVNFKTG
560 570 580 590 600
DNRISILGVL TGLPDSGSYM EHRFAGPRAI SIIGLKSGTR DLTENNEWGH
610 620 630 640 650
LAGLEGEKKE VYTEEGSKKV KWEKDGKRKP LTWYKTYFET PEGVNAVAIR
660 670 680 690 700
MKAMGKGLIW VNGIGVGRYW MSFLSPLGEP TQTEYHIPRS FMKGEKKKNM
710 720 730 740 750
LVILEEEPGV KLESIDFVLV NRDTICSNVG EDYPVSVKSW KREGPKIVSR
760 770 780 790 800
SKDMRLKAVM RCPPEKQMVE VQFASFGDPT GTCGNFTMGK CSASKSKEVV
810 820 830 840 850
EKECLGRNYC SIVVARETFG DKGCPEIVKT LAVQVKCEKK EGKQDEKKKK
860 870 880
EDKDEEEEDD EDDDEEEEEE DKENKDTKDM ENKNQDM
Length:887
Mass (Da):101,222
Last modified:July 9, 2007 - v2
Checksum:i58486CB4B949508D
GO

Sequence cautioni

The sequence AEE86951.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80523.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571L → F in CAB64750 (Ref. 1) Curated
Sequence conflicti70 – 701H → D in CAB64750 (Ref. 1) Curated
Sequence conflicti286 – 2861V → A in CAB64750 (Ref. 1) Curated
Sequence conflicti627 – 6271K → E in CAB64750 (Ref. 1) Curated
Sequence conflicti653 – 6531A → G in CAB64750 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270310 mRNA. Translation: CAB64750.1.
AL035540 Genomic DNA. Translation: CAB37515.1. Sequence problems.
AL161593 Genomic DNA. Translation: CAB80523.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86951.1. Sequence problems.
PIRiT05687.
RefSeqiNP_195571.2. NM_120020.3.
UniGeneiAt.70242.

Genome annotation databases

EnsemblPlantsiAT4G38590.1; AT4G38590.1; AT4G38590.
GeneIDi830016.
KEGGiath:AT4G38590.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270310 mRNA. Translation: CAB64750.1.
AL035540 Genomic DNA. Translation: CAB37515.1. Sequence problems.
AL161593 Genomic DNA. Translation: CAB80523.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86951.1. Sequence problems.
PIRiT05687.
RefSeqiNP_195571.2. NM_120020.3.
UniGeneiAt.70242.

3D structure databases

ProteinModelPortaliQ9SCU8.
SMRiQ9SCU8. Positions 41-709.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ9SCU8.
PRIDEiQ9SCU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G38590.1; AT4G38590.1; AT4G38590.
GeneIDi830016.
KEGGiath:AT4G38590.

Organism-specific databases

GeneFarmi517. 90.
TAIRiAT4G38590.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9SCU8.
PhylomeDBiQ9SCU8.

Enzyme and pathway databases

BioCyciARA:GQT-2802-MONOMER.

Gene expression databases

GenevestigatoriQ9SCU8.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006104. Glyco_hydro_2_N.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
IPR000922. Lectin_gal-bd_dom.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF02140. Gal_Lectin. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGA14_ARATH
AccessioniPrimary (citable) accession number: Q9SCU8
Secondary accession number(s): F4JUE4, Q9SZN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2007
Last sequence update: July 9, 2007
Last modified: January 6, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.