Reviewed,
UniProtKB/Swiss-Prot Q9SCP2 (PEL12_ARATH)
Last modified
November 3, 2009.
Version 64.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Probable pectate lyase 12 EC=4.2.2.2 | ||||
| Gene names |
| ||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 483 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends. |
| Cofactor | Binds 1 calcium ion. Required for its activity By similarity. |
| Pathway | Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5. |
| Sequence similarities | Belongs to the polysaccharide lyase 1 family. |
| Caution | It is uncertain whether Met-1 or Met-2 is the initiator. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Lyase |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Cellular component | anchored to membrane Traceable author statement. Source: TAIR |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | Potential | ||||||
| Chain | 25 – 483 | 459 | Probable pectate lyase 12 | PRO_0000024876 | |||||
Sites | |||||||||
| Active site | 300 | 1 | Potential | ||||||
| Metal binding | 220 | 1 | Calcium By similarity | ||||||
| Metal binding | 244 | 1 | Calcium By similarity | ||||||
| Metal binding | 248 | 1 | Calcium By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 27 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 50 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 17 | 1 | L → F in AAM61400. Ref.2 | ||||||
| Sequence conflict | 21 – 22 | 2 | MV → KG in AAM61400. Ref.2 | ||||||
| Sequence conflict | 25 | 1 | M → I in AAM61400. Ref.2 | ||||||
| Sequence conflict | 28 | 1 | R → T in AAM61400. Ref.2 | ||||||
| Sequence conflict | 33 | 1 | I → V in AAM61400. Ref.2 | ||||||
Sequences
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References
| [1] | "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.  Tabata S.Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AL132958 Genomic DNA. Translation: CAB64222.1. Different initiation. AY084835 mRNA. Translation: AAM61400.1. Different initiation. | |
| IPI | IPI00541244. |
| PIR | T46165. |
| RefSeq | NP_566979.1. |
| UniGene | At.28000 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1JRG based on UniProtKB P29155. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | PL1. Polysaccharide Lyase Family 1. |
Genome annotation databases | |
| GeneID | 824485. |
| GenomeReviews | Gene locus AT3G53190 in contig BA000014_GR. |
| KEGG | ath:AT3G53190. |
| NMPDR | fig|3702.1.peg.16594. |
Organism-specific databases | |
| TAIR | At3g53190. |
Phylogenomic databases | |
| OMA | KAYSVEP. |
Enzyme and pathway databases | |
| BRENDA | 4.2.2.2. 302. |
Gene expression databases | |
| ArrayExpress | Q9SCP2. |
| Genevestigator | Q9SCP2. |
| GermOnline | AT3G53190. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view] |
| Gene3D | G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. |
| Pfam | PF00544. Pec_lyase_C. 1 hit. [Graphical view] |
| PRINTS | PR00807. AMBALLERGEN. |
| SMART | SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PEL12_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9SCP2 Secondary accession number(s): Q8LFH8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
