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Protein

Ubiquitin carboxyl-terminal hydrolase 26

Gene

UBP26

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Deubiquitinates H2BK143ub1 of histone H2B.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151Nucleophile
Active sitei359 – 3591Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT3G49600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 26 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 26
Short name:
AtUBP26
Ubiquitin thioesterase 26
Ubiquitin-specific-processing protease 26
Gene namesi
Name:UBP26
Synonyms:SUP32
Ordered Locus Names:At3g49600
ORF Names:T9C5.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G49600.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleolus Source: TAIR
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi115 – 1151C → S: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10671067Ubiquitin carboxyl-terminal hydrolase 26PRO_0000293492Add
BLAST

Proteomic databases

PaxDbiQ9SCJ9.
PRIDEiQ9SCJ9.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, stems, leaves and inflorescences.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G49600.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SCJ9.
SMRiQ9SCJ9. Positions 107-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 442337USPAdd
BLAST
Domaini503 – 59593DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini610 – 711102DUSP 2PROSITE-ProRule annotationAdd
BLAST
Domaini738 – 861124DUSP 3PROSITE-ProRule annotationAdd
BLAST
Domaini948 – 103184Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi392 – 43241Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 3 DUSP domains.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000090485.
InParanoidiQ9SCJ9.
KOiK11858.
OMAiHPGQKYY.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SCJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPNTRNKN KRQRPDAVDS SSQILRKIHE ANDVTDDDIN QLFMIWKPVC
60 70 80 90 100
QGCRVNTRDN PNCFCGLVPP LNGSRKSGLW QKTSEIIQSL GPDPTLDRRD
110 120 130 140 150
SESTPAGLTN LGATCYANSI LQCLYMNTAF REGVFSVEVH VLKQNPVLDQ
160 170 180 190 200
IARLFAQLHA SQKSFVDSDA FVKTLELDNG VQQDTHEFLT LLLSLLERCL
210 220 230 240 250
LHSGVKAKTI VQDLFSGSVS HVTTCSKCGR DSEASSKMED FYALELNVKG
260 270 280 290 300
LKSLDASLND YLSLEQLNGD NQYFCGSCNA RVDATRCIKL RTLPPVITFQ
310 320 330 340 350
LKRCIFLPKT TAKKKITSSF SFPQVLDMGS RLAESSQNKL TYDLSAVLIH
360 370 380 390 400
KGSAVNSGHY VAHIKDEKTG LWWEFDDEHV SELGKRPCNE ASSSTPQSES
410 420 430 440 450
NGTASSGNIT DGIQSGSSDC RSAIKSEVFS SSDAYMLMYS LRCDKQENQE
460 470 480 490 500
GQKENPIDIT KGEVKQLKGG YLPKHLSEWI NNMNAVFLES CKQYNLRKEK
510 520 530 540 550
ELNALTERRQ EVRTILSEAA VQSLEEQYFW ISTDWLRLWA DTTLPPALDN
560 570 580 590 600
TPLLCSHGKV HASKVNCMKR ISELAWIKLE SKFNGGPKLG KGDYCRDCLM
610 620 630 640 650
DGARMVVSSD SYRDRRTFMK SIANDVLSGK CEDGMYYISR AWLQQWIKRK
660 670 680 690 700
NLDAPTEADA GPTNAITCNH GELMPEQAPG AKRVVVPENF WSFLFEDALK
710 720 730 740 750
VMSEDTLDCT CFPVDSSQCC HCTEVLSEVA CFEDSLRTLK VKQRQNHEKL
760 770 780 790 800
ATGKGIPLTP QSRYFLLPSP WLVQWRIYIN MTGKNSSSAP EPERLDGVIN
810 820 830 840 850
TLKCKKHTRL LERLPELVCR RGSYFQKNPS TDKLTIIPEL DWKYFCDEWG
860 870 880 890 900
GLMENGISAF IEVGNTDQSS SPDVIDLEKD SSPDDNMDVD AQQLILRASP
910 920 930 940 950
EICEECIGER ESCELMQKLS YSEGDVFVCF VRGKEAPKAM LEASDSSFEV
960 970 980 990 1000
DRRTSKRSRR TNYGNLTSLK VSATTTVYQL KMMIWELLGV MKENQELHKG
1010 1020 1030 1040 1050
SKVIDQESAT LADMNIFPGD RLWVRDTEMH EHRDIADELC EKKPGAQDIE
1060
EGFRGTLLTG NISSEAC
Length:1,067
Mass (Da):119,934
Last modified:May 3, 2011 - v3
Checksum:iC0338D496C035573
GO

Sequence cautioni

The sequence CAB62464.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti935 – 9351E → G in AAG42764 (PubMed:11115897).Curated
Sequence conflicti989 – 9891G → E in AAG42764 (PubMed:11115897).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302674 mRNA. Translation: AAG42764.1.
AL132964 Genomic DNA. Translation: CAB62464.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78564.1.
PIRiT46237.
RefSeqiNP_566922.1. NM_114820.3.
UniGeneiAt.22294.

Genome annotation databases

EnsemblPlantsiAT3G49600.1; AT3G49600.1; AT3G49600.
GeneIDi824122.
KEGGiath:AT3G49600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302674 mRNA. Translation: AAG42764.1.
AL132964 Genomic DNA. Translation: CAB62464.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78564.1.
PIRiT46237.
RefSeqiNP_566922.1. NM_114820.3.
UniGeneiAt.22294.

3D structure databases

ProteinModelPortaliQ9SCJ9.
SMRiQ9SCJ9. Positions 107-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G49600.1.

Proteomic databases

PaxDbiQ9SCJ9.
PRIDEiQ9SCJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G49600.1; AT3G49600.1; AT3G49600.
GeneIDi824122.
KEGGiath:AT3G49600.

Organism-specific databases

TAIRiAT3G49600.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000090485.
InParanoidiQ9SCJ9.
KOiK11858.
OMAiHPGQKYY.

Enzyme and pathway databases

BioCyciARA:AT3G49600-MONOMER.

Miscellaneous databases

PROiQ9SCJ9.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
  2. "Control of DNA methylation and heterochromatic silencing by histone H2B deubiquitination."
    Sridhar V.V., Kapoor A., Zhang K., Zhu J., Zhou T., Hasegawa P.M., Bressan R.A., Zhu J.-K.
    Nature 447:735-738(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-115, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP26_ARATH
AccessioniPrimary (citable) accession number: Q9SCJ9
Secondary accession number(s): Q9FPS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: May 3, 2011
Last modified: July 22, 2015
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.