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Q9SCG9

- PLY1_CUPAR

UniProt

Q9SCG9 - PLY1_CUPAR

Protein

Pectate lyase 1

Gene
N/A
Organism
Cupressus arizonica (Arizona cypress) (Callitropsis arizonica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Has pectate lyase activity.By similarity

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

    Cofactori

    Binds 1 calcium ion.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi170 – 1701CalciumBy similarity
    Metal bindingi194 – 1941CalciumBy similarity
    Metal bindingi198 – 1981CalciumBy similarity
    Active sitei250 – 2501Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase 1 (EC:4.2.2.2)
    Alternative name(s):
    Major pollen allergen Cup a 1
    Allergen: Cup a 1
    OrganismiCupressus arizonica (Arizona cypress) (Callitropsis arizonica)
    Taxonomic identifieri49011 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeHesperocyparis

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei256. Cup a 1.
    3232. Cup a 1.0101.
    895. Cup a 1.02.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 367346Pectate lyase 1PRO_0000212997Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 45By similarity
    Disulfide bondi128 ↔ 147By similarity
    Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi306 ↔ 312By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00544. Pec_lyase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiSM00656. Amb_all. 1 hit.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9SCG9-1 [UniParc]FASTAAdd to Basket

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    MASPCLVAVL VFLCAIVSCY SDNPIDSCWR GDSNWDQNRM KLADCVVGFG    50
    SSTMGGKGGE IYTVTSSEDN PVNPTPGTLR YGATREKALW IIFSQNMNIK 100
    LQMPLYVAGY KTIDGRGADV HLGNGGPCLF MRKASHVILH GLHIHGCNTS 150
    VLGDVLVSES IGVEPVHAQD GDAITMRNVT NAWIDHNSLS DCSDGLIDVT 200
    LGSTGITISN NHFFNHHKVM LLGHDDTYDD DKSMKVTVAF NQFGPNAGQR 250
    MPRARYGLVH VANNNYDQWN IYAIGGSSNP TILSEGNSFT APNESYKKEV 300
    TKRIGCETTS ACANWVWRST RDAFTNGAYF VSSGKAEDTN IYNSNEAFKV 350
    ENGNAAPQLT QNAGVVA 367
    Length:367
    Mass (Da):39,776
    Last modified:April 16, 2014 - v2
    Checksum:i0138EE8653B1ABDB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521S → L in CAC37790. 1 PublicationCurated
    Sequence conflicti68 – 681E → D in CAC37790. 1 PublicationCurated
    Sequence conflicti108 – 1081A → N in ABK78766. (PubMed:19902388)Curated
    Sequence conflicti119 – 1191D → V in CAB62551. (PubMed:11122214)Curated
    Sequence conflicti133 – 1331K → T in CAC37790. 1 PublicationCurated
    Sequence conflicti232 – 2321K → I in CAC37790. 1 PublicationCurated
    Sequence conflicti293 – 2931N → S in CAC37790. 1 PublicationCurated
    Sequence conflicti308 – 3081T → S in CAC37790. 1 PublicationCurated
    Sequence conflicti319 – 3191S → F in CAC37790. 1 PublicationCurated
    Sequence conflicti338 – 3381D → E in CAC37790. 1 PublicationCurated
    Sequence conflicti367 – 3671A → T in CAC37790. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278498 mRNA. Translation: CAC37790.2.
    EF079863 mRNA. Translation: ABK78766.1.
    AJ243570 mRNA. Translation: CAB62551.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278498 mRNA. Translation: CAC37790.2 .
    EF079863 mRNA. Translation: ABK78766.1 .
    AJ243570 mRNA. Translation: CAB62551.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 256. Cup a 1.
    3232. Cup a 1.0101.
    895. Cup a 1.02.
    CAZyi PL1. Polysaccharide Lyase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00544. Pec_lyase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00807. AMBALLERGEN.
    SMARTi SM00656. Amb_all. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of Cupressus Arizonica major allergen."
      Butteroni C., Di Felice G., Pini C.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pollen.
    2. "A modified protocol for RNA isolation from high polysaccharide containing Cupressus arizonica pollen. Applications for RT-PCR and phage display library construction."
      Pico de Coana Y., Parody N., Fernandez-Caldas E., Alonso C.
      Mol. Biotechnol. 44:127-132(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-367.
      Tissue: Pollen.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-367, ALLERGEN.

    Entry informationi

    Entry nameiPLY1_CUPAR
    AccessioniPrimary (citable) accession number: Q9SCG9
    Secondary accession number(s): A0T2M2, Q93XL6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3