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Q9SCG9 (PLY1_CUPAR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pectate lyase 1

EC=4.2.2.2
Alternative name(s):
Major pollen allergen Cup a 1
Allergen=Cup a 1
OrganismCupressus arizonica (Arizona cypress) (Callitropsis arizonica)
Taxonomic identifier49011 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeHesperocyparis

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has pectate lyase activity By similarity.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Allergenic properties

Causes an allergic reaction in human. Ref.3

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
   DiseaseAllergen
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processpectin catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 367346Pectate lyase 1
PRO_0000212997

Sites

Active site2501 Potential
Metal binding1701Calcium By similarity
Metal binding1941Calcium By similarity
Metal binding1981Calcium By similarity

Amino acid modifications

Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 45 By similarity
Disulfide bond128 ↔ 147 By similarity
Disulfide bond306 ↔ 312 By similarity

Experimental info

Sequence conflict521S → L in CAC37790. Ref.1
Sequence conflict681E → D in CAC37790. Ref.1
Sequence conflict1081A → N in ABK78766. Ref.2
Sequence conflict1191D → V in CAB62551. Ref.3
Sequence conflict1331K → T in CAC37790. Ref.1
Sequence conflict2321K → I in CAC37790. Ref.1
Sequence conflict2931N → S in CAC37790. Ref.1
Sequence conflict3081T → S in CAC37790. Ref.1
Sequence conflict3191S → F in CAC37790. Ref.1
Sequence conflict3381D → E in CAC37790. Ref.1
Sequence conflict3671A → T in CAC37790. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9SCG9 [UniParc].

Last modified April 16, 2014. Version 2.
Checksum: 0138EE8653B1ABDB

FASTA36739,776
        10         20         30         40         50         60 
MASPCLVAVL VFLCAIVSCY SDNPIDSCWR GDSNWDQNRM KLADCVVGFG SSTMGGKGGE 

        70         80         90        100        110        120 
IYTVTSSEDN PVNPTPGTLR YGATREKALW IIFSQNMNIK LQMPLYVAGY KTIDGRGADV 

       130        140        150        160        170        180 
HLGNGGPCLF MRKASHVILH GLHIHGCNTS VLGDVLVSES IGVEPVHAQD GDAITMRNVT 

       190        200        210        220        230        240 
NAWIDHNSLS DCSDGLIDVT LGSTGITISN NHFFNHHKVM LLGHDDTYDD DKSMKVTVAF 

       250        260        270        280        290        300 
NQFGPNAGQR MPRARYGLVH VANNNYDQWN IYAIGGSSNP TILSEGNSFT APNESYKKEV 

       310        320        330        340        350        360 
TKRIGCETTS ACANWVWRST RDAFTNGAYF VSSGKAEDTN IYNSNEAFKV ENGNAAPQLT 


QNAGVVA 

« Hide

References

[1]"Cloning of Cupressus Arizonica major allergen."
Butteroni C., Di Felice G., Pini C.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pollen.
[2]"A modified protocol for RNA isolation from high polysaccharide containing Cupressus arizonica pollen. Applications for RT-PCR and phage display library construction."
Pico de Coana Y., Parody N., Fernandez-Caldas E., Alonso C.
Mol. Biotechnol. 44:127-132(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-367.
Tissue: Pollen.
[3]"Molecular cloning of major allergen from Cupressus arizonica pollen: Cup a 1."
Aceituno E., Del Pozo V., Minguez A., Arrieta I., Cortegano I., Cardaba B., Gallardo S., Rojo M., Palomino P., Lahoz C.
Clin. Exp. Allergy 30:1750-1758(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-367, ALLERGEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ278498 mRNA. Translation: CAC37790.2.
EF079863 mRNA. Translation: ABK78766.1.
AJ243570 mRNA. Translation: CAB62551.1.

3D structure databases

ProteinModelPortalQ9SCG9.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome256. Cup a 1.
3232. Cup a 1.0101.
895. Cup a 1.02.
CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00545; UER00824.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMSSF51126. SSF51126. 1 hit.
ProtoNetSearch...

Entry information

Entry namePLY1_CUPAR
AccessionPrimary (citable) accession number: Q9SCG9
Secondary accession number(s): A0T2M2, Q93XL6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: April 16, 2014
Last modified: June 11, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Allergens

Nomenclature of allergens and list of entries