UGT85B1

Functionⁱ

Involved in the biosynthesis of the cyanogenic glucoside dhurrin. Prevents the dissociation and release of toxic hydrogen cyanide. Mandelonitrile, p-hydroxymandelonitrile, benzyl alcohol, benzoic acid and geraniol, but not hydroquinone(1,4-benzenediol), alpha-terpinol, linalool or farnesol are utilized as acceptor substrates. UDP-glucose, but not UDP-xylose or UDP-glucuronic acid can be used as sugar donor.3 Publications

Catalytic activityⁱ

UDP-alpha-D-glucose + (S)-4-hydroxymandelonitrile = UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside.3 Publications

Enzyme regulationⁱ

Inhibited by p-hydroxybenzaldehyde.

Kineticsⁱ

Pathwayⁱ: dhurrin biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes dhurrin from L-tyrosine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:

Tyrosine N-monooxygenase (CYP79A1)
4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1)
Cyanohydrin beta-glucosyltransferase (UGT85B1)

This subpathway is part of the pathway dhurrin biosynthesis, which is itself part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dhurrin from L-tyrosine, the pathway dhurrin biosynthesis and in Secondary metabolite biosynthesis.

GO - Molecular functionⁱ

cyanohydrin beta-glucosyltransferase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

dhurrin biosynthetic process Source: UniProtKB-UniPathway

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase

Molecular function

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCycⁱ	MetaCyc:MONOMER-523.
BRENDAⁱ	2.4.1.85. 5768.
SABIO-RKⁱ	Q9SBL1.
UniPathwayⁱ	UPA00757; UER00746.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT1. Glycosyltransferase Family 1.

CAZyⁱ

GT1. Glycosyltransferase Family 1.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cyanohydrin beta-glucosyltransferase (EC:2.4.1.853 Publications) Alternative name(s): UDP-glucose-p-hydroxymandelonitrile glucosyltransferase
Gene namesⁱ	Name:UGT85B1 Synonyms:HMNGT
Organismⁱ	Sorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifierⁱ	4558 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Sorghinae › Sorghum

Subcellular locationⁱ

Note:

In the presence of CYP79A1 and CYP71E1, moves toward the surface of the ER membranes in order to form a metabolon.

GO - Cellular componentⁱ

endoplasmic reticulum membrane Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	201	R → A: 20-fold reduction in activity. 1 Publication	1
Mutagenesisⁱ	391	S → A: 185-fold reduction in activity. 1 Publication	1
Mutagenesisⁱ	410	E → A: 225-fold reduction in activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000365608	1 – 492	Cyanohydrin beta-glucosyltransferaseAdd BLAST		492

Post-translational modificationⁱ

The N-terminus is partially blocked.

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9SBL1. baseline.

ExpressionAtlasⁱ

Q9SBL1. baseline.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9SBL1.
SMRⁱ	Q9SBL1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	6 – 11	Poly-Pro		6
Compositional biasⁱ	136 – 140	Poly-Ala		5

Sequence similaritiesⁱ

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1192. Eukaryota. COG1819. LUCA.
HOGENOMⁱ	HOG000237564.
KEGG Orthology (KO) More...KOⁱ	K13030.
OMAⁱ	KEMMAGE.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093607YV.

Family and domain databases

InterProⁱ	View protein in InterPro IPR002213. UDP_glucos_trans.
PANTHERⁱ	PTHR11926. PTHR11926. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00201. UDPGT. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9SBL1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGSNAPPPPT PHVVLVPFPG QGHVAPLMQL ARLLHARGAR VTFVYTQYNY 
        60         70         80         90        100
RRLLRAKGEA AVRPPATSSA RFRIEVIDDG LSLSVPQNDV GGLVDSLRKN 
       110        120        130        140        150
CLHPFRALLR RLGQEVEGQD APPVTCVVGD VVMTFAAAAA REAGIPEVQF 
       160        170        180        190        200
FTASACGLLG YLHYGELVER GLVPFRDASL LADDDYLDTP LEWVPGMSHM 
       210        220        230        240        250
RLRDMPTFCR TTDPDDVMVS ATLQQMESAA GSKALILNTL YELEKDVVDA 
       260        270        280        290        300
LAAFFPPIYT VGPLAEVIAS SDSASAGLAA MDISIWQEDT RCLSWLDGKP 
       310        320        330        340        350
AGSVVYVNFG SMAVMTAAQA REFALGLASC GSPFLWVKRP DVVEGEEVLL 
       360        370        380        390        400
PEALLDEVAR GRGLVVPWCP QAAVLKHAAV GLFVSHCGWN SLLEATAAGQ 
       410        420        430        440        450
PVLAWPCHGE QTTNCRQLCE VWGNGAQLPR EVESGAVARL VREMMVGDLG 
       460        470        480        490 
KEKRAKAAEW KAAAEAAARK GGASWRNVER VVNDLLLVGG KQ

Length:492

Mass (Da):52,916

Last modified:May 1, 2000 - v1

Checksum:ⁱABC6A57AC51E9BE7

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF199453 mRNA. Translation: AAF17077.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_002463518.1. XM_002463473.1.
UniGeneⁱ	Sbi.14307.

Genome annotation databases

GeneIDⁱ	8060874.
KEGGⁱ	sbi:SORBI_01g001220.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF199453 mRNA. Translation: AAF17077.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_002463518.1. XM_002463473.1.
UniGeneⁱ	Sbi.14307.

3D structure databases

ProteinModelPortalⁱ	Q9SBL1.
SMRⁱ	Q9SBL1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT1. Glycosyltransferase Family 1.

CAZyⁱ

GT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	8060874.
KEGGⁱ	sbi:SORBI_01g001220.

Phylogenomic databases

eggNOGⁱ	KOG1192. Eukaryota. COG1819. LUCA.
HOGENOMⁱ	HOG000237564.
KEGG Orthology (KO) More...KOⁱ	K13030.
OMAⁱ	KEMMAGE.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093607YV.

Enzyme and pathway databases

UniPathwayⁱ	UPA00757; UER00746.
BioCycⁱ	MetaCyc:MONOMER-523.
BRENDAⁱ	2.4.1.85. 5768.
SABIO-RKⁱ	Q9SBL1.

Gene expression databases

ExpressionAtlasⁱ	Q9SBL1. baseline.

ExpressionAtlasⁱ

Q9SBL1. baseline.

Family and domain databases

InterProⁱ	View protein in InterPro IPR002213. UDP_glucos_trans.
PANTHERⁱ	PTHR11926. PTHR11926. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00201. UDPGT. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HMNGT_SORBI
Accessionⁱ	Q9SBL1Primary (citable) accession number: Q9SBL1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
	Last sequence update:	May 1, 2000
	Last modified:	July 5, 2017
	This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9SBL1 (HMNGT_SORBI)

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Cyanohydrin beta-glucosyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dhurrin biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Enzyme regulationⁱ

Kineticsⁱ

Pathwayⁱ: dhurrin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ