UniProtKB - Q9SBL1 (HMNGT_SORBI)
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Protein
Cyanohydrin beta-glucosyltransferase
Gene
UGT85B1
Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Functioni
Involved in the biosynthesis of the cyanogenic glucoside dhurrin. Prevents the dissociation and release of toxic hydrogen cyanide. Mandelonitrile, p-hydroxymandelonitrile, benzyl alcohol, benzoic acid and geraniol, but not hydroquinone(1,4-benzenediol), alpha-terpinol, linalool or farnesol are utilized as acceptor substrates. UDP-glucose, but not UDP-xylose or UDP-glucuronic acid can be used as sugar donor.3 Publications
Catalytic activityi
UDP-alpha-D-glucose + (S)-4-hydroxymandelonitrile = UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside.3 Publications
Enzyme regulationi
Inhibited by p-hydroxybenzaldehyde.
Kineticsi
- KM=0.14 mM for geraniol2 Publications
- KM=0.13 mM for beta-citronellol2 Publications
- KM=1.13 mM for nerol2 Publications
- KM=0.66 mM for 1-hexanol2 Publications
- KM=0.73 mM for cis-3-hexen-1-ol2 Publications
- KM=0.84 mM for mandelonitrile2 Publications
- KM=6.33 mM for 2-hydroxy-3-methoxybenzyl alcohol2 Publications
- KM=0.69 mM for 3-methyl-3-buten-1-ol2 Publications
- KM=10.3 mM for 3-methyl-2-buten-1-ol2 Publications
- KM=0.8 mM for UDP-glucose2 Publications
: dhurrin biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes dhurrin from L-tyrosine.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Tyrosine N-monooxygenase (CYP79A1)
- 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1)
- Cyanohydrin beta-glucosyltransferase (UGT85B1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dhurrin from L-tyrosine, the pathway dhurrin biosynthesis and in Secondary metabolite biosynthesis.
GO - Molecular functioni
- cyanohydrin beta-glucosyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- dhurrin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-523 |
BRENDAi | 2.4.1.85 5768 |
SABIO-RKi | Q9SBL1 |
UniPathwayi | UPA00757; UER00746 |
Protein family/group databases
CAZyi | GT1 Glycosyltransferase Family 1 |
Names & Taxonomyi
Protein namesi | Recommended name: Cyanohydrin beta-glucosyltransferase (EC:2.4.1.853 Publications)Alternative name(s): UDP-glucose-p-hydroxymandelonitrile glucosyltransferase |
Gene namesi | Name:UGT85B1 Synonyms:HMNGT |
Organismi | Sorghum bicolor (Sorghum) (Sorghum vulgare) |
Taxonomic identifieri | 4558 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Sorghinae › Sorghum |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 201 | R → A: 20-fold reduction in activity. 1 Publication | 1 | |
Mutagenesisi | 391 | S → A: 185-fold reduction in activity. 1 Publication | 1 | |
Mutagenesisi | 410 | E → A: 225-fold reduction in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000365608 | 1 – 492 | Cyanohydrin beta-glucosyltransferaseAdd BLAST | 492 |
Post-translational modificationi
The N-terminus is partially blocked.
Structurei
3D structure databases
ProteinModelPortali | Q9SBL1 |
SMRi | Q9SBL1 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 6 – 11 | Poly-Pro | 6 | |
Compositional biasi | 136 – 140 | Poly-Ala | 5 |
Sequence similaritiesi
Belongs to the UDP-glycosyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG1192 Eukaryota COG1819 LUCA |
HOGENOMi | HOG000237564 |
KOi | K13030 |
OMAi | KEMMAGE |
OrthoDBi | EOG093607YV |
Family and domain databases
InterProi | View protein in InterPro IPR002213 UDP_glucos_trans |
Pfami | View protein in Pfam PF00201 UDPGT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SBL1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSNAPPPPT PHVVLVPFPG QGHVAPLMQL ARLLHARGAR VTFVYTQYNY
60 70 80 90 100
RRLLRAKGEA AVRPPATSSA RFRIEVIDDG LSLSVPQNDV GGLVDSLRKN
110 120 130 140 150
CLHPFRALLR RLGQEVEGQD APPVTCVVGD VVMTFAAAAA REAGIPEVQF
160 170 180 190 200
FTASACGLLG YLHYGELVER GLVPFRDASL LADDDYLDTP LEWVPGMSHM
210 220 230 240 250
RLRDMPTFCR TTDPDDVMVS ATLQQMESAA GSKALILNTL YELEKDVVDA
260 270 280 290 300
LAAFFPPIYT VGPLAEVIAS SDSASAGLAA MDISIWQEDT RCLSWLDGKP
310 320 330 340 350
AGSVVYVNFG SMAVMTAAQA REFALGLASC GSPFLWVKRP DVVEGEEVLL
360 370 380 390 400
PEALLDEVAR GRGLVVPWCP QAAVLKHAAV GLFVSHCGWN SLLEATAAGQ
410 420 430 440 450
PVLAWPCHGE QTTNCRQLCE VWGNGAQLPR EVESGAVARL VREMMVGDLG
460 470 480 490
KEKRAKAAEW KAAAEAAARK GGASWRNVER VVNDLLLVGG KQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF199453 mRNA Translation: AAF17077.1 |
RefSeqi | XP_002463518.1, XM_002463473.1 |
UniGenei | Sbi.14307 |
Genome annotation databases
EnsemblPlantsi | EER90516; EER90516; SORBI_3001G012400 |
GeneIDi | 8060874 |
Gramenei | EER90516; EER90516; SORBI_3001G012400 |
KEGGi | sbi:8060874 |
Similar proteinsi
Entry informationi
Entry namei | HMNGT_SORBI | |
Accessioni | Q9SBL1Primary (citable) accession number: Q9SBL1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 3, 2009 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 28, 2018 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |