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Protein

Cyanohydrin beta-glucosyltransferase

Gene

UGT85B1

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the cyanogenic glucoside dhurrin. Prevents the dissociation and release of toxic hydrogen cyanide. Mandelonitrile, p-hydroxymandelonitrile, benzyl alcohol, benzoic acid and geraniol, but not hydroquinone(1,4-benzenediol), alpha-terpinol, linalool or farnesol are utilized as acceptor substrates. UDP-glucose, but not UDP-xylose or UDP-glucuronic acid can be used as sugar donor.3 Publications

Catalytic activityi

UDP-D-glucose + (S)-4-hydroxymandelonitrile = UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside.

Enzyme regulationi

Inhibited by p-hydroxybenzaldehyde.

Kineticsi

  1. KM=0.14 mM for geraniol2 Publications
  2. KM=0.13 mM for beta-citronellol2 Publications
  3. KM=1.13 mM for nerol2 Publications
  4. KM=0.66 mM for 1-hexanol2 Publications
  5. KM=0.73 mM for cis-3-hexen-1-ol2 Publications
  6. KM=0.84 mM for mandelonitrile2 Publications
  7. KM=6.33 mM for 2-hydroxy-3-methoxybenzyl alcohol2 Publications
  8. KM=0.69 mM for 3-methyl-3-buten-1-ol2 Publications
  9. KM=10.3 mM for 3-methyl-2-buten-1-ol2 Publications
  10. KM=0.8 mM for UDP-glucose2 Publications

    Pathwayi: dhurrin biosynthesis

    This protein is involved in step 3 of the subpathway that synthesizes dhurrin from L-tyrosine.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Tyrosine N-monooxygenase (CYP79A1)
    2. 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1)
    3. Cyanohydrin beta-glucosyltransferase (UGT85B1)
    This subpathway is part of the pathway dhurrin biosynthesis, which is itself part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes dhurrin from L-tyrosine, the pathway dhurrin biosynthesis and in Secondary metabolite biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-523.
    BRENDAi2.4.1.85. 5768.
    SABIO-RKQ9SBL1.
    UniPathwayiUPA00757; UER00746.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyanohydrin beta-glucosyltransferase (EC:2.4.1.85)
    Alternative name(s):
    UDP-glucose-p-hydroxymandelonitrile glucosyltransferase
    Gene namesi
    Name:UGT85B1
    Synonyms:HMNGT
    OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
    Taxonomic identifieri4558 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeSorghinaeSorghum

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi201 – 2011R → A: 20-fold reduction in activity. 1 Publication
    Mutagenesisi391 – 3911S → A: 185-fold reduction in activity. 1 Publication
    Mutagenesisi410 – 4101E → A: 225-fold reduction in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Cyanohydrin beta-glucosyltransferasePRO_0000365608Add
    BLAST

    Post-translational modificationi

    The N-terminus is partially blocked.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ9SBL1. baseline and differential.

    Interactioni

    Protein-protein interaction databases

    STRINGi4558.Sb01g001220.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SBL1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi6 – 116Poly-Pro
    Compositional biasi136 – 1405Poly-Ala

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237564.
    KOiK13030.
    OMAiDISIWQE.
    OrthoDBiEOG093607YV.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SBL1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSNAPPPPT PHVVLVPFPG QGHVAPLMQL ARLLHARGAR VTFVYTQYNY
    60 70 80 90 100
    RRLLRAKGEA AVRPPATSSA RFRIEVIDDG LSLSVPQNDV GGLVDSLRKN
    110 120 130 140 150
    CLHPFRALLR RLGQEVEGQD APPVTCVVGD VVMTFAAAAA REAGIPEVQF
    160 170 180 190 200
    FTASACGLLG YLHYGELVER GLVPFRDASL LADDDYLDTP LEWVPGMSHM
    210 220 230 240 250
    RLRDMPTFCR TTDPDDVMVS ATLQQMESAA GSKALILNTL YELEKDVVDA
    260 270 280 290 300
    LAAFFPPIYT VGPLAEVIAS SDSASAGLAA MDISIWQEDT RCLSWLDGKP
    310 320 330 340 350
    AGSVVYVNFG SMAVMTAAQA REFALGLASC GSPFLWVKRP DVVEGEEVLL
    360 370 380 390 400
    PEALLDEVAR GRGLVVPWCP QAAVLKHAAV GLFVSHCGWN SLLEATAAGQ
    410 420 430 440 450
    PVLAWPCHGE QTTNCRQLCE VWGNGAQLPR EVESGAVARL VREMMVGDLG
    460 470 480 490
    KEKRAKAAEW KAAAEAAARK GGASWRNVER VVNDLLLVGG KQ
    Length:492
    Mass (Da):52,916
    Last modified:May 1, 2000 - v1
    Checksum:iABC6A57AC51E9BE7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF199453 mRNA. Translation: AAF17077.1.
    RefSeqiXP_002463518.1. XM_002463473.1.
    UniGeneiSbi.14307.

    Genome annotation databases

    EnsemblPlantsiSb01g001220.1; Sb01g001220.1; Sb01g001220.
    GeneIDi8060874.
    GrameneiSb01g001220.1; Sb01g001220.1; Sb01g001220.
    KEGGisbi:SORBI_01g001220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF199453 mRNA. Translation: AAF17077.1.
    RefSeqiXP_002463518.1. XM_002463473.1.
    UniGeneiSbi.14307.

    3D structure databases

    ProteinModelPortaliQ9SBL1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi4558.Sb01g001220.1.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiSb01g001220.1; Sb01g001220.1; Sb01g001220.
    GeneIDi8060874.
    GrameneiSb01g001220.1; Sb01g001220.1; Sb01g001220.
    KEGGisbi:SORBI_01g001220.

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237564.
    KOiK13030.
    OMAiDISIWQE.
    OrthoDBiEOG093607YV.

    Enzyme and pathway databases

    UniPathwayiUPA00757; UER00746.
    BioCyciMetaCyc:MONOMER-523.
    BRENDAi2.4.1.85. 5768.
    SABIO-RKQ9SBL1.

    Gene expression databases

    ExpressionAtlasiQ9SBL1. baseline and differential.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 2 hits.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHMNGT_SORBI
    AccessioniPrimary (citable) accession number: Q9SBL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 3, 2009
    Last sequence update: May 1, 2000
    Last modified: September 7, 2016
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.