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Protein

Respiratory burst oxidase homolog protein B

Gene

RBOHB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi184 – 1841CalciumBy similarity
Metal bindingi186 – 1861CalciumBy similarity
Metal bindingi188 – 1881CalciumBy similarity
Metal bindingi190 – 1901Calcium; via carbonyl oxygenBy similarity
Metal bindingi195 – 1951CalciumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi184 – 19512PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • NAD(P)H oxidase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • response to heat Source: TAIR
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G09090-MONOMER.
ARA:GQT-596-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Protein family/group databases

PeroxiBasei3283. AtRboh02.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein B (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHB
Short name:
AtRBOHB
Gene namesi
Name:RBOHB
Ordered Locus Names:At1g09090
ORF Names:F7G19.3, F7G19.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09090.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 297297CytoplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31821Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini319 – 38365ExtracellularSequence analysisAdd
BLAST
Transmembranei384 – 40421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini405 – 43935CytoplasmicSequence analysisAdd
BLAST
Transmembranei440 – 46021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini461 – 48222ExtracellularSequence analysisAdd
BLAST
Transmembranei483 – 50321Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini504 – 5118CytoplasmicSequence analysis
Transmembranei512 – 52918Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini530 – 659130ExtracellularSequence analysisAdd
BLAST
Transmembranei660 – 68021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini681 – 843163CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 843843Respiratory burst oxidase homolog protein BPRO_0000313754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SBI0.
PRIDEiQ9SBI0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9SBI0. baseline and differential.
GenevisibleiQ9SBI0. AT.

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G09090.2.

Structurei

3D structure databases

ProteinModelPortaliQ9SBI0.
SMRiQ9SBI0. Positions 83-246, 645-843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 20636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini215 – 25036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini336 – 495160Ferric oxidoreductaseAdd
BLAST
Domaini534 – 657124FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 1229EF-hand-like 1By similarity
Regioni148 – 15912EF-hand-like 2By similarityAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ9SBI0.
OMAiDHNPIKR.
OrthoDBiEOG093601IF.
PhylomeDBiQ9SBI0.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SBI0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MREEEMESSS EGETNKISRC KATGSDNPDE DYVEITLEVR DETINTMKAK
60 70 80 90 100
ATLRSVLSGR LKTMVKSLSF ASRRLDRSKS FGAMFALRGL RFIAKNDAVG
110 120 130 140 150
RGWDEVAMRF DKLAVEGKLP KSKFGHCIGM VESSEFVNEL FEALVRRRGT
160 170 180 190 200
TSSSITKTEL FEFWEQITGN SFDDRLQIFF DMVDKNLDGR ITGDEVKEII
210 220 230 240 250
ALSASANKLS KIKENVDEYA ALIMEELDRD NLGYIELHNL ETLLLQVPSQ
260 270 280 290 300
SNNSPSSANK RALNKMLSQK LIPTKDRNPV KRFAMNISYF FLENWKRIWV
310 320 330 340 350
LTLWISICIT LFTWKFLQYK RKTVFEVMGY CVTVAKGSAE TLKFNMALIL
360 370 380 390 400
LPVCRNTITW LRTKSKLIGS VVPFDDNINF HKVVAFGIAV GIGLHAISHL
410 420 430 440 450
ACDFPRLLHA KNVEFEPMKK FFGDERPENY GWFMKGTDGW TGVTMVVLML
460 470 480 490 500
VAYVLAQSWF RRNRANLPKS LKRLTGFNAF WYSHHLFVIV YVLLIVHGYF
510 520 530 540 550
VYLSKEWYHK TTWMYLAVPV LLYAFERLIR AFRPGAKAVK VLKVAVYPGN
560 570 580 590 600
VLSLYMSKPK GFKYTSGQYI YINCSDVSPL QWHPFSITSA SGDDYLSVHI
610 620 630 640 650
RTLGDWTSQL KSLYSKVCQL PSTSQSGLFI ADIGQANNIT RFPRLLIDGP
660 670 680 690 700
YGAPAQDYRN YDVLLLVGLG IGATPLISII RDVLNNIKNQ NSIERGTNQH
710 720 730 740 750
IKNYVATKRA YFYWVTREQG SLEWFSEVMN EVAEYDSEGM IELHNYCTSV
760 770 780 790 800
YEEGDARSAL ITMLQSLHHA KSGIDIVSGT RVRTHFARPN WRSVFKHVAV
810 820 830 840
NHVNQRVGVF YCGNTCIIGE LKRLAQDFSR KTTTKFEFHK ENF
Length:843
Mass (Da):96,390
Last modified:May 1, 2000 - v1
Checksum:iEBA11C6C74FED340
GO
Isoform 2 (identifier: Q9SBI0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     618-621: CQLP → TIFQ
     622-843: Missing.

Note: No experimental confirmation available.
Show »
Length:621
Mass (Da):71,204
Checksum:i60687CE142FF26F6
GO

Sequence cautioni

The sequence AAB70398 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAB70399 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei618 – 6214CQLP → TIFQ in isoform 2. 1 PublicationVSP_030130
Alternative sequencei622 – 843222Missing in isoform 2. 1 PublicationVSP_030131Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055354 Genomic DNA. Translation: AAC39476.1.
AC000106 Genomic DNA. Translation: AAB70398.1. Sequence problems.
AC000106 Genomic DNA. Translation: AAB70399.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28394.1.
BT005716 mRNA. Translation: AAO64136.1.
PIRiA86223.
B86223.
RefSeqiNP_172383.3. NM_100780.3.
NP_973799.1. NM_202070.1. [Q9SBI0-1]
UniGeneiAt.10379.
At.42241.

Genome annotation databases

EnsemblPlantsiAT1G09090.2; AT1G09090.2; AT1G09090. [Q9SBI0-1]
GeneIDi837430.
KEGGiath:AT1G09090.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055354 Genomic DNA. Translation: AAC39476.1.
AC000106 Genomic DNA. Translation: AAB70398.1. Sequence problems.
AC000106 Genomic DNA. Translation: AAB70399.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28394.1.
BT005716 mRNA. Translation: AAO64136.1.
PIRiA86223.
B86223.
RefSeqiNP_172383.3. NM_100780.3.
NP_973799.1. NM_202070.1. [Q9SBI0-1]
UniGeneiAt.10379.
At.42241.

3D structure databases

ProteinModelPortaliQ9SBI0.
SMRiQ9SBI0. Positions 83-246, 645-843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G09090.2.

Protein family/group databases

PeroxiBasei3283. AtRboh02.

Proteomic databases

PaxDbiQ9SBI0.
PRIDEiQ9SBI0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09090.2; AT1G09090.2; AT1G09090. [Q9SBI0-1]
GeneIDi837430.
KEGGiath:AT1G09090.

Organism-specific databases

TAIRiAT1G09090.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ9SBI0.
OMAiDHNPIKR.
OrthoDBiEOG093601IF.
PhylomeDBiQ9SBI0.

Enzyme and pathway databases

BioCyciARA:AT1G09090-MONOMER.
ARA:GQT-596-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

PROiQ9SBI0.

Gene expression databases

ExpressionAtlasiQ9SBI0. baseline and differential.
GenevisibleiQ9SBI0. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHB_ARATH
AccessioniPrimary (citable) accession number: Q9SBI0
Secondary accession number(s): O04020, O04021, Q84TJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.