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Protein

NADPH--cytochrome P450 reductase 1

Gene

ATR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and dichloroindophenol.UniRule annotation5 Publications

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotation1 PublicationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotation1 PublicationNote: Binds 1 FMN per monomer.UniRule annotation

Kineticsi

  1. KM=2.2 µM for NADPH (at pH 7.0 and 25 degrees Celsius)3 Publications
  2. KM=21.9 µM for NADPH (at pH 7.7 and 28 degrees Celsius)3 Publications
  3. KM=2.0 µM for NADPH (at pH 7.25 and 25 degrees Celsius)3 Publications
  4. KM=17 µM for cytochrome c (at pH 7.0 and 25 degrees Celsius)3 Publications
  5. KM=24.3 µM for cytochrome c (at pH 7.7 and 28 degrees Celsius)3 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei219FMNUniRule annotation1
    Binding sitei310NADPUniRule annotation1
    Binding sitei551NADPUniRule annotation1
    Binding sitei654NADPUniRule annotation1
    Binding sitei692FADUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi91 – 96FMNUniRule annotation6
    Nucleotide bindingi146 – 149FMNUniRule annotation4
    Nucleotide bindingi184 – 193FMNUniRule annotation10
    Nucleotide bindingi470 – 473FADUniRule annotation4
    Nucleotide bindingi488 – 490FADUniRule annotation3
    Nucleotide bindingi504 – 507FADUniRule annotation4
    Nucleotide bindingi612 – 613NADPUniRule annotation2
    Nucleotide bindingi618 – 622NADPUniRule annotation5

    GO - Molecular functioni

    GO - Biological processi

    • phenylpropanoid metabolic process Source: TAIR
    • response to abscisic acid Source: TAIR
    • response to oxidative stress Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Phenylpropanoid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciARA:AT4G24520-MONOMER.
    MetaCyc:MONOMER-2101.
    ReactomeiR-ATH-211897. Cytochrome P450 - arranged by substrate type.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase 1UniRule annotation (EC:1.6.2.4UniRule annotation)
    Short name:
    CPR 1UniRule annotation
    Short name:
    P450R 1UniRule annotation
    Gene namesi
    Name:ATR1
    Synonyms:AR1
    Ordered Locus Names:At4g24520
    ORF Names:F22K18.280
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G24520.

    Subcellular locationi

    • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 26LumenalUniRule annotationAdd BLAST25
    Transmembranei27 – 47HelicalUniRule annotationAdd BLAST21
    Topological domaini48 – 692CytoplasmicUniRule annotationAdd BLAST645

    GO - Cellular componenti

    • cytosol Source: TAIR
    • endoplasmic reticulum Source: TAIR
    • endoplasmic reticulum membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00004168392 – 692NADPH--cytochrome P450 reductase 1Add BLAST691

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylthreonineCombined sources1
    Modified residuei249PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9SB48.

    PTM databases

    iPTMnetiQ9SB48.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, flowers and siliques.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SB48. baseline and differential.
    GenevisibleiQ9SB48. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi13843. 2 interactors.
    STRINGi3702.AT4G24520.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SB48.
    SMRiQ9SB48.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini85 – 235Flavodoxin-likeUniRule annotationAdd BLAST151
    Domaini290 – 537FAD-binding FR-typeUniRule annotationAdd BLAST248

    Sequence similaritiesi

    Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
    In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
    Contains 1 FAD-binding FR-type domain.UniRule annotation
    Contains 1 flavodoxin-like domain.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000282027.
    InParanoidiQ9SB48.
    KOiK00327.
    OMAiISEQRGI.
    OrthoDBiEOG093604WR.
    PhylomeDBiQ9SB48.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_03212. NCPR. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin-like.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like_dom.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9SB48-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MTSALYASDL FKQLKSIMGT DSLSDDVVLV IATTSLALVA GFVVLLWKKT
    60 70 80 90 100
    TADRSGELKP LMIPKSLMAK DEDDDLDLGS GKTRVSIFFG TQTGTAEGFA
    110 120 130 140 150
    KALSEEIKAR YEKAAVKVID LDDYAADDDQ YEEKLKKETL AFFCVATYGD
    160 170 180 190 200
    GEPTDNAARF YKWFTEENER DIKLQQLAYG VFALGNRQYE HFNKIGIVLD
    210 220 230 240 250
    EELCKKGAKR LIEVGLGDDD QSIEDDFNAW KESLWSELDK LLKDEDDKSV
    260 270 280 290 300
    ATPYTAVIPE YRVVTHDPRF TTQKSMESNV ANGNTTIDIH HPCRVDVAVQ
    310 320 330 340 350
    KELHTHESDR SCIHLEFDIS RTGITYETGD HVGVYAENHV EIVEEAGKLL
    360 370 380 390 400
    GHSLDLVFSI HADKEDGSPL ESAVPPPFPG PCTLGTGLAR YADLLNPPRK
    410 420 430 440 450
    SALVALAAYA TEPSEAEKLK HLTSPDGKDE YSQWIVASQR SLLEVMAAFP
    460 470 480 490 500
    SAKPPLGVFF AAIAPRLQPR YYSISSSPRL APSRVHVTSA LVYGPTPTGR
    510 520 530 540 550
    IHKGVCSTWM KNAVPAEKSH ECSGAPIFIR ASNFKLPSNP STPIVMVGPG
    560 570 580 590 600
    TGLAPFRGFL QERMALKEDG EELGSSLLFF GCRNRQMDFI YEDELNNFVD
    610 620 630 640 650
    QGVISELIMA FSREGAQKEY VQHKMMEKAA QVWDLIKEEG YLYVCGDAKG
    660 670 680 690
    MARDVHRTLH TIVQEQEGVS SSEAEAIVKK LQTEGRYLRD VW
    Length:692
    Mass (Da):76,766
    Last modified:May 1, 2000 - v1
    Checksum:i7DD77E418CCF2FA6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti161Y → S in CAA46814 (PubMed:9235908).Curated1
    Sequence conflicti477 – 480SPRL → CQDW in CAA46814 (PubMed:9235908).Curated4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66016 mRNA. Translation: CAA46814.1.
    AL035356 Genomic DNA. Translation: CAA23011.1.
    AL161561 Genomic DNA. Translation: CAB79362.1.
    CP002687 Genomic DNA. Translation: AEE84919.1.
    AY054688 mRNA. Translation: AAK96879.1.
    BT008426 mRNA. Translation: AAP37785.1.
    PIRiT05582.
    RefSeqiNP_194183.1. NM_118585.4. [Q9SB48-1]
    UniGeneiAt.144.
    At.70123.

    Genome annotation databases

    EnsemblPlantsiAT4G24520.1; AT4G24520.1; AT4G24520. [Q9SB48-1]
    GeneIDi828554.
    GrameneiAT4G24520.1; AT4G24520.1; AT4G24520.
    KEGGiath:AT4G24520.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66016 mRNA. Translation: CAA46814.1.
    AL035356 Genomic DNA. Translation: CAA23011.1.
    AL161561 Genomic DNA. Translation: CAB79362.1.
    CP002687 Genomic DNA. Translation: AEE84919.1.
    AY054688 mRNA. Translation: AAK96879.1.
    BT008426 mRNA. Translation: AAP37785.1.
    PIRiT05582.
    RefSeqiNP_194183.1. NM_118585.4. [Q9SB48-1]
    UniGeneiAt.144.
    At.70123.

    3D structure databases

    ProteinModelPortaliQ9SB48.
    SMRiQ9SB48.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi13843. 2 interactors.
    STRINGi3702.AT4G24520.1.

    PTM databases

    iPTMnetiQ9SB48.

    Proteomic databases

    PaxDbiQ9SB48.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G24520.1; AT4G24520.1; AT4G24520. [Q9SB48-1]
    GeneIDi828554.
    GrameneiAT4G24520.1; AT4G24520.1; AT4G24520.
    KEGGiath:AT4G24520.

    Organism-specific databases

    TAIRiAT4G24520.

    Phylogenomic databases

    eggNOGiKOG1158. Eukaryota.
    COG0369. LUCA.
    HOGENOMiHOG000282027.
    InParanoidiQ9SB48.
    KOiK00327.
    OMAiISEQRGI.
    OrthoDBiEOG093604WR.
    PhylomeDBiQ9SB48.

    Enzyme and pathway databases

    BioCyciARA:AT4G24520-MONOMER.
    MetaCyc:MONOMER-2101.
    ReactomeiR-ATH-211897. Cytochrome P450 - arranged by substrate type.

    Miscellaneous databases

    PROiQ9SB48.

    Gene expression databases

    ExpressionAtlasiQ9SB48. baseline and differential.
    GenevisibleiQ9SB48. AT.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_03212. NCPR. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin-like.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like_dom.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNCPR1_ARATH
    AccessioniPrimary (citable) accession number: Q9SB48
    Secondary accession number(s): Q39035
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: May 1, 2000
    Last modified: November 30, 2016
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.