Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine-containing phosphotransfer protein 3

Gene

AHP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.4 Publications

GO - Molecular functioni

  • histidine phosphotransfer kinase activity Source: TAIR
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • phosphorelay signal transduction system Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cytokinin signaling pathway, Stress response, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-containing phosphotransfer protein 3
Gene namesi
Name:AHP3
Synonyms:ATHP2
Ordered Locus Names:At5g39340
ORF Names:MUL8.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G39340.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749291 – 155Histidine-containing phosphotransfer protein 3Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei82PhosphohistidinePROSITE-ProRule annotation1

Post-translational modificationi

Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additional intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9SAZ5.

Expressioni

Tissue specificityi

Strongly expressed in roots. Detected also in flowers, leaves, siliques and stems.2 Publications

Inductioni

By salt, cold and drought stress.1 Publication

Gene expression databases

GenevisibleiQ9SAZ5. AT.

Interactioni

Subunit structurei

Interacts with the B-type response regulators ARR1, ARR2 and ARR9. Binds to ETR1, AHK2, AHK3, AHK4, AHK5 and CKI1.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHK2Q9C5U23EBI-1100711,EBI-1100634
AHK3Q9C5U12EBI-1100711,EBI-1100653
ARR1Q940D03EBI-1100711,EBI-1100998
ARR14Q8L9Y33EBI-1100711,EBI-1100737
ARR16Q9SHC22EBI-1100711,EBI-1100982
ARR5Q9SB043EBI-1100711,EBI-1100883
ARR7Q9ZWS74EBI-1100711,EBI-1100917
ARR9O803665EBI-1100711,EBI-1100950
At1g59940Q29PQ82EBI-1100711,EBI-1100853

GO - Molecular functioni

  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi19181. 27 interactors.
IntActiQ9SAZ5. 26 interactors.
STRINGi3702.AT5G39340.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SAZ5.
SMRiQ9SAZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 147HPtPROSITE-ProRule annotationAdd BLAST108

Domaini

Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.

Sequence similaritiesi

Contains 1 HPt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9SAZ5.
KOiK14490.
OMAiCDSQNYE.
OrthoDBiEOG09360S25.
PhylomeDBiQ9SAZ5.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SAZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTLIAQLQR RFCDFTISLY HQGFLDDQFT ELKKLQDECS PDFVAEVVTL
60 70 80 90 100
FFEDCEKLIS NMARALDQTG NVDFKLVGSS VHQLKGSSSS VGAKRVKGLC
110 120 130 140 150
VTLKECCDSQ NYEGCVRCLQ QVDIEYKTLK AKLQDLFNLE QQIVQAGGRI

PQVDI
Length:155
Mass (Da):17,479
Last modified:July 5, 2004 - v2
Checksum:iF3B26DCFA3904E0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 7MDTLIAQ → MGTK in BAA37111 (PubMed:9804162).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012569 mRNA. Translation: BAA37111.1.
AB015143 mRNA. Translation: BAA36337.1.
AB009054 Genomic DNA. Translation: BAB11008.1.
CP002688 Genomic DNA. Translation: AED94422.1.
AY050944 mRNA. Translation: AAK93621.1.
AY091174 mRNA. Translation: AAM14113.1.
UniGeneiAt.25339.

Genome annotation databases

EnsemblPlantsiAT5G39340.1; AT5G39340.1; AT5G39340.
GrameneiAT5G39340.1; AT5G39340.1; AT5G39340.
KEGGiath:AT5G39340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012569 mRNA. Translation: BAA37111.1.
AB015143 mRNA. Translation: BAA36337.1.
AB009054 Genomic DNA. Translation: BAB11008.1.
CP002688 Genomic DNA. Translation: AED94422.1.
AY050944 mRNA. Translation: AAK93621.1.
AY091174 mRNA. Translation: AAM14113.1.
UniGeneiAt.25339.

3D structure databases

ProteinModelPortaliQ9SAZ5.
SMRiQ9SAZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19181. 27 interactors.
IntActiQ9SAZ5. 26 interactors.
STRINGi3702.AT5G39340.1.

Proteomic databases

PaxDbiQ9SAZ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G39340.1; AT5G39340.1; AT5G39340.
GrameneiAT5G39340.1; AT5G39340.1; AT5G39340.
KEGGiath:AT5G39340.

Organism-specific databases

TAIRiAT5G39340.

Phylogenomic databases

eggNOGiKOG4747. Eukaryota.
COG2198. LUCA.
HOGENOMiHOG000272680.
InParanoidiQ9SAZ5.
KOiK14490.
OMAiCDSQNYE.
OrthoDBiEOG09360S25.
PhylomeDBiQ9SAZ5.

Miscellaneous databases

PROiQ9SAZ5.

Gene expression databases

GenevisibleiQ9SAZ5. AT.

Family and domain databases

Gene3Di1.20.120.160. 1 hit.
InterProiIPR008207. Sig_transdc_His_kin_Hpt_dom.
[Graphical view]
PfamiPF01627. Hpt. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 1 hit.
PROSITEiPS50894. HPT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAHP3_ARATH
AccessioniPrimary (citable) accession number: Q9SAZ5
Secondary accession number(s): Q9ZWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.