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Protein

1-aminocyclopropane-1-carboxylate synthase 6

Gene

ACS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. Involved in bacterial flagellin-induced ethylene production.1 Publication

Miscellaneous

The stability of ACS proteins, and the regulation of such stability, play a central role in ethylene biosynthesis. The phosphorylation of serine residues on the C-terminus increases protein stability.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.1 Publication

Cofactori

Kineticsi

  1. KM=23 µM for AdoMet
  1. Vmax=120.60 µM/h/mg enzyme

pH dependencei

Optimum pH is 7.3.

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei96SubstrateBy similarity1

GO - Molecular functioni

  • 1-aminocyclopropane-1-carboxylate synthase activity Source: TAIR
  • identical protein binding Source: IntAct
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • cell division Source: TAIR
  • cellular response to iron ion Source: TAIR
  • defense response Source: UniProtKB-KW
  • ethylene biosynthetic process Source: TAIR
  • fruit ripening Source: UniProtKB-KW
  • phloem or xylem histogenesis Source: TAIR
  • response to auxin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to mechanical stimulus Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionLyase
Biological processEthylene biosynthesis, Fruit ripening, Plant defense
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi4.4.1.14 399
SABIO-RKQ9SAR0
UniPathwayiUPA00384; UER00562

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 6 (EC:4.4.1.14)
Short name:
ACC synthase 6
Alternative name(s):
S-adenosyl-L-methionine methylthioadenosine-lyase 6
Gene namesi
Name:ACS6
Synonyms:ACC6
Ordered Locus Names:At4g11280
ORF Names:F8L21.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G11280
TAIRilocus:2128298 AT4G11280

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi480S → D: 40-fold increase in ethylene production; when associated with D-483 and D-488. 1 Publication1
Mutagenesisi483S → D: 40-fold increase in ethylene production; when associated with D-480 and D-488. 1 Publication1
Mutagenesisi488S → D: 40-fold increase in ethylene production; when associated with D-480 and D-483. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239001 – 4951-aminocyclopropane-1-carboxylate synthase 6Add BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei280N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei480Phosphoserine1 Publication1
Modified residuei483Phosphoserine1 Publication1
Modified residuei488Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated on serine residue by MAP kinase (MPK6).1 Publication
May be processed at its C-terminus.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SAR0

PTM databases

iPTMnetiQ9SAR0

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Inductioni

By indole-3-acetic acid (IAA) and cycloheximide (CHX). By auxin. By treatment with ozone.3 Publications

Gene expression databases

ExpressionAtlasiQ9SAR0 baseline and differential
GenevisibleiQ9SAR0 AT

Interactioni

Subunit structurei

Homodimer and heterodimer. In vivo, the relevance of heterodimerization with other ACS enzymes is however unsure (By similarity). Interacts with GRF3.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi12029, 4 interactors
IntActiQ9SAR0, 4 interactors
STRINGi3702.AT4G11280.1

Structurei

3D structure databases

ProteinModelPortaliQ9SAR0
SMRiQ9SAR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256 Eukaryota
COG0436 LUCA
HOGENOMiHOG000011234
InParanoidiQ9SAR0
KOiK20772
OMAiQNTEWID
OrthoDBiEOG0936082N
PhylomeDBiQ9SAR0

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SAR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAFATEKKQ DLNLLSKIAS GDGHGENSSY FDGWKAYEEN PFHPIDRPDG
60 70 80 90 100
VIQMGLAENQ LCGDLMRKWV LKHPEASICT SEGVNQFSDI AIFQDYHGLP
110 120 130 140 150
EFRQAVAKFM EKTRNNKVKF DPDRIVMSGG ATGAHETVAF CLANPGDGFL
160 170 180 190 200
VPTPYYPGFD RDLRWRTGVN LVPVTCHSSN GFKITVEALE AAYENARKSN
210 220 230 240 250
IPVKGLLVTN PSNPLGTTLD RECLKSLVNF TNDKGIHLIA DEIYAATTFG
260 270 280 290 300
QSEFISVAEV IEEIEDCNRD LIHIVYSLSK DMGLPGLRVG IVYSYNDRVV
310 320 330 340 350
QIARKMSSFG LVSSQTQHLI AKMLSDEEFV DEFIRESKLR LAARHAEITT
360 370 380 390 400
GLDGLGIGWL KAKAGLFLWM DLRNLLKTAT FDSETELWRV IVHQVKLNVS
410 420 430 440 450
PGGSFHCHEP GWFRVCFANM DHKTMETALE RIRVFTSQLE EETKPMAATT
460 470 480 490
MMAKKKKKCW QSNLRLSFSD TRRFDDGFFS PHSPVPPSPL VRAQT
Length:495
Mass (Da):55,524
Last modified:July 19, 2004 - v2
Checksum:i7FA94D3EABBCB4AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56L → F in AAC32251 (Ref. 5) Curated1
Sequence conflicti433R → K in AAC63850 (PubMed:10080689).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL096882 Genomic DNA Translation: CAB51412.1
AL161531 Genomic DNA Translation: CAB81229.1
CP002687 Genomic DNA Translation: AEE82992.1
AF361097 mRNA Translation: AAK27237.1
AF428292 mRNA Translation: AAL16124.1
BT000487 mRNA Translation: AAN18056.1
U73786 mRNA Translation: AAC63850.1
U79524 mRNA Translation: AAC32251.1
PIRiT13019
RefSeqiNP_192867.1, NM_117199.2
UniGeneiAt.3654

Genome annotation databases

EnsemblPlantsiAT4G11280.1; AT4G11280.1; AT4G11280
GeneIDi826730
GrameneiAT4G11280.1; AT4G11280.1; AT4G11280
KEGGiath:AT4G11280

Similar proteinsi

Entry informationi

Entry namei1A16_ARATH
AccessioniPrimary (citable) accession number: Q9SAR0
Secondary accession number(s): O82719, Q9SUT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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