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Protein

Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic

Gene

GAPCP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in plastidial glycolytic pathway and plays a specific role in glycolytic energy production in non-green plastids and chloroplasts. Essential for breakdown of starch to form sucrose for export to non-photosynthetic tissues, and to generate primary metabolites for anabolic pathways such as fatty acid and amino acid synthesis. Plays an important role in plant development by providing substrates for the phosphorylated pathway of serine biosynthesis in roots. Plays a crucial role in pollen development. Functionally redundant with GAPCP2.2 Publications

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118NADBy similarity1
Binding sitei164NAD; via carbonyl oxygenBy similarity1
Active sitei236NucleophilePROSITE-ProRule annotation1
Sitei263Activates thiol group during catalysisBy similarity1
Binding sitei266Glyceraldehyde 3-phosphateBy similarity1
Binding sitei318Glyceraldehyde 3-phosphateBy similarity1
Binding sitei400NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 97NADBy similarity2

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: TAIR
  • NAD binding Source: InterPro
  • NADP binding Source: InterPro
  • zinc ion binding Source: TAIR

GO - Biological processi

  • amino acid homeostasis Source: TAIR
  • anther wall tapetum development Source: TAIR
  • carbohydrate metabolic process Source: TAIR
  • glucose metabolic process Source: InterPro
  • glycolytic process Source: UniProtKB-KW
  • pollen development Source: TAIR
  • primary root development Source: TAIR
  • response to cytokinin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.12. 399.
ReactomeiR-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic (EC:1.2.1.12)
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase of plastid 1
NAD-dependent glyceraldehydephosphate dehydrogenase chloroplastic 1
Gene namesi
Name:GAPCP1
Ordered Locus Names:At1g79530
ORF Names:T8K14.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G79530.

Subcellular locationi

GO - Cellular componenti

  • chloroplast stroma Source: UniProtKB-SubCell
  • membrane Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Gapcp1 and gapcp2 double mutants have severe dwarf phenotypes with arrested root development and male sterility. Pollen grains show shrunken and collapsed forms and cannot germinate.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi236C → G: Loss of activity. 1 Publication1
Mutagenesisi263H → A: Loss of activity. 1 Publication1
Mutagenesisi311K → A: No effect on the activity. 1 Publication1
Mutagenesisi318R → E: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 69ChloroplastCombined sourcesAdd BLAST69
ChainiPRO_000042240770 – 422Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplasticAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SAJ6.
PRIDEiQ9SAJ6.
ProMEXiQ9SAJ6.

PTM databases

iPTMnetiQ9SAJ6.
SwissPalmiQ9SAJ6.

Expressioni

Tissue specificityi

Expressed in shoot and root vasculature, leaf veins and vascular tissue of flowers and siliques.2 Publications

Inductioni

Repressed by darkness, but not by sucrose.1 Publication

Gene expression databases

GenevisibleiQ9SAJ6. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi29510. 2 interactors.
IntActiQ9SAJ6. 2 interactors.
STRINGi3702.AT1G79530.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SAJ6.
SMRiQ9SAJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni295 – 296Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
InParanoidiQ9SAJ6.
KOiK00134.
OMAiGIAKGFM.
OrthoDBiEOG09360F5Y.
PhylomeDBiQ9SAJ6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SAJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSSLLRSA ASYTVAAPRP DFFSSPASDH SKVLSSLGFS RNLKPSRFSS
60 70 80 90 100
GISSSLQNGN ARSVQPIKAT ATEVPSAVRR SSSSGKTKVG INGFGRIGRL
110 120 130 140 150
VLRIATSRDD IEVVAVNDPF IDAKYMAYML KYDSTHGNFK GSINVIDDST
160 170 180 190 200
LEINGKKVNV VSKRDPSEIP WADLGADYVV ESSGVFTTLS KAASHLKGGA
210 220 230 240 250
KKVIISAPSA DAPMFVVGVN EHTYQPNMDI VSNASCTTNC LAPLAKVVHE
260 270 280 290 300
EFGILEGLMT TVHATTATQK TVDGPSMKDW RGGRGASQNI IPSSTGAAKA
310 320 330 340 350
VGKVLPELNG KLTGMAFRVP TSNVSVVDLT CRLEKGASYE DVKAAIKHAS
360 370 380 390 400
EGPLKGILGY TDEDVVSNDF VGDSRSSIFD ANAGIGLSKS FVKLVSWYDN
410 420
EWGYSNRVLD LIEHMALVAA SH
Length:422
Mass (Da):44,831
Last modified:May 1, 2000 - v1
Checksum:i3160AF8CDC2B30B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007202 Genomic DNA. Translation: AAD30223.1.
CP002684 Genomic DNA. Translation: AEE36260.1.
AK117920 mRNA. Translation: BAC42558.1.
AF348583 mRNA. Translation: AAK15554.1.
PIRiF96826.
RefSeqiNP_178071.1. NM_106601.4.
UniGeneiAt.17116.

Genome annotation databases

EnsemblPlantsiAT1G79530.1; AT1G79530.1; AT1G79530.
GeneIDi844291.
GrameneiAT1G79530.1; AT1G79530.1; AT1G79530.
KEGGiath:AT1G79530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007202 Genomic DNA. Translation: AAD30223.1.
CP002684 Genomic DNA. Translation: AEE36260.1.
AK117920 mRNA. Translation: BAC42558.1.
AF348583 mRNA. Translation: AAK15554.1.
PIRiF96826.
RefSeqiNP_178071.1. NM_106601.4.
UniGeneiAt.17116.

3D structure databases

ProteinModelPortaliQ9SAJ6.
SMRiQ9SAJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29510. 2 interactors.
IntActiQ9SAJ6. 2 interactors.
STRINGi3702.AT1G79530.1.

PTM databases

iPTMnetiQ9SAJ6.
SwissPalmiQ9SAJ6.

Proteomic databases

PaxDbiQ9SAJ6.
PRIDEiQ9SAJ6.
ProMEXiQ9SAJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G79530.1; AT1G79530.1; AT1G79530.
GeneIDi844291.
GrameneiAT1G79530.1; AT1G79530.1; AT1G79530.
KEGGiath:AT1G79530.

Organism-specific databases

TAIRiAT1G79530.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
InParanoidiQ9SAJ6.
KOiK00134.
OMAiGIAKGFM.
OrthoDBiEOG09360F5Y.
PhylomeDBiQ9SAJ6.

Enzyme and pathway databases

BRENDAi1.2.1.12. 399.
ReactomeiR-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ9SAJ6.

Gene expression databases

GenevisibleiQ9SAJ6. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PP1_ARATH
AccessioniPrimary (citable) accession number: Q9SAJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-dependent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.