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Protein

Alpha-glucan water dikinase 1, chloroplastic

Gene

GWD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the incorporation of phosphate into starch-like alpha-glucan, mostly at the C-6 position of glucose units. Acts as an overall regulator of starch mobilization. Required for starch degradation, suggesting that the phosphate content of starch regulates its degradability.1 Publication

Catalytic activityi

ATP + alpha-glucan + H2O = AMP + phospho-alpha-glucan + phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1004Tele-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

  • alpha-glucan, water dikinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cold acclimation Source: TAIR
  • response to symbiotic fungus Source: TAIR
  • starch catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G10760-MONOMER.
MetaCyc:AT1G10760-MONOMER.

Protein family/group databases

CAZyiCBM45. Carbohydrate-Binding Module Family 45.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucan water dikinase 1, chloroplastic (EC:2.7.9.4)
Alternative name(s):
Protein starch excess 1
Protein starch-related R1
Gene namesi
Name:GWD1
Synonyms:R1, SEX1
Ordered Locus Names:At1g10760
ORF Names:F20B24.19, T16B5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10760.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1268G → E in sex1-1; induces an excess of starch in leaves after a long period of darkness. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 75ChloroplastCombined sourcesAdd BLAST75
ChainiPRO_000002356576 – 1399Alpha-glucan water dikinase 1, chloroplasticAdd BLAST1324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76N-acetylvalineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SAC6.
PRIDEiQ9SAC6.

PTM databases

iPTMnetiQ9SAC6.

Expressioni

Developmental stagei

The level of protein does not vary in a circadian rhythm and is stable throughout day and night (at protein level).1 Publication

Gene expression databases

GenevisibleiQ9SAC6. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi22859. 5 interactors.
IntActiQ9SAC6. 1 interactor.
MINTiMINT-8331304.
STRINGi3702.AT1G10760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SAC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain contains the alpha-glucan binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the ATP binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHGM. Eukaryota.
ENOG410XUE8. LUCA.
HOGENOMiHOG000265165.
InParanoidiQ9SAC6.
KOiK08244.
OMAiCLKGWNQ.
OrthoDBiEOG093600AI.
PhylomeDBiQ9SAC6.

Family and domain databases

Gene3Di3.30.1490.20. 2 hits.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF01326. PPDK_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SAC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNSVVHNLL NRGLIRPLNF EHQNKLNSSV YQTSTANPAL GKIGRSKLYG
60 70 80 90 100
KGLKQAGRSL VTETGGRPLS FVPRAVLAMD PQAAEKFSLD GNIDLLVEVT
110 120 130 140 150
STTVREVNIQ IAYTSDTLFL HWGAILDNKE NWVLPSRSPD RTQNFKNSAL
160 170 180 190 200
RTPFVKSGGN SHLKLEIDDP AIHAIEFLIF DESRNKWYKN NGQNFHINLP
210 220 230 240 250
TERNVKQNVS VPEDLVQIQA YLRWERKGKQ MYNPEKEKEE YEAARTELRE
260 270 280 290 300
EMMRGASVED LRAKLLKKDN SNESPKSNGT SSSGREEKKK VSKQPERKKN
310 320 330 340 350
YNTDKIQRKG RDLTKLIYKH VADFVEPESK SSSEPRSLTT LEIYAKAKEE
360 370 380 390 400
QETTPVFSKK TFKLEGSAIL VFVTKLSGKT KIHVATDFKE PVTLHWALSQ
410 420 430 440 450
KGGEWLDPPS DILPPNSLPV RGAVDTKLTI TSTDLPSPVQ TFELEIEGDS
460 470 480 490 500
YKGMPFVLNA GERWIKNNDS DFYVDFAKEE KHVQKDYGDG KGTAKHLLDK
510 520 530 540 550
IADLESEAQK SFMHRFNIAA DLVDEAKSAG QLGFAGILVW MRFMATRQLV
560 570 580 590 600
WNKNYNVKPR EISKAQDRLT DLLQDVYASY PEYRELLRMI MSTVGRGGEG
610 620 630 640 650
DVGQRIRDEI LVIQRKNDCK GGIMEEWHQK LHNNTSPDDV VICQALMDYI
660 670 680 690 700
KSDFDLSVYW KTLNDNGITK ERLLSYDRAI HSEPNFRGEQ KDGLLRDLGH
710 720 730 740 750
YMRTLKAVHS GADLESAIQN CMGYQDDGEG FMVGVQINPV SGLPSGYPDL
760 770 780 790 800
LRFVLEHVEE KNVEPLLEGL LEARQELRPL LLKSHDRLKD LLFLDLALDS
810 820 830 840 850
TVRTAIERGY EQLNDAGPEK IMYFISLVLE NLALSSDDNE DLIYCLKGWQ
860 870 880 890 900
FALDMCKSKK DHWALYAKSV LDRSRLALAS KAERYLEILQ PSAEYLGSCL
910 920 930 940 950
GVDQSAVSIF TEEIIRAGSA AALSSLVNRL DPVLRKTANL GSWQVISPVE
960 970 980 990 1000
VVGYVIVVDE LLTVQNKTYD RPTIIVANRV RGEEEIPDGA VAVLTPDMPD
1010 1020 1030 1040 1050
VLSHVSVRAR NGKICFATCF DSGILSDLQG KDGKLLSLQP TSADVVYKEV
1060 1070 1080 1090 1100
NDSELSSPSS DNLEDAPPSI SLVKKQFAGR YAISSEEFTS DLVGAKSRNI
1110 1120 1130 1140 1150
GYLKGKVPSW VGIPTSVALP FGVFEKVISE KANQAVNDKL LVLKKTLDEG
1160 1170 1180 1190 1200
DQGALKEIRQ TLLGLVAPPE LVEELKSTMK SSDMPWPGDE GEQRWEQAWA
1210 1220 1230 1240 1250
AIKKVWASKW NERAYFSTRK VKLDHDYLCM AVLVQEVINA DYAFVIHTTN
1260 1270 1280 1290 1300
PSSGDSSEIY AEVVKGLGET LVGAYPGRSL SFICKKNNLD SPLVLGYPSK
1310 1320 1330 1340 1350
PIGLFIRRSI IFRSDSNGED LEGYAGAGLY DSVPMDEEDQ VVLDYTTDPL
1360 1370 1380 1390
ITDLSFQKKV LSDIARAGDA IEKLYGTAQD IEGVIRDGKL YVVQTRPQV
Length:1,399
Mass (Da):156,582
Last modified:January 4, 2005 - v2
Checksum:i1FE9285376B479EB
GO

Sequence cautioni

The sequence AAD31337 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF17665 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAK49609 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK96541 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312027 mRNA. Translation: AAG47821.1.
AC007354 Genomic DNA. Translation: AAD31337.1. Sequence problems.
AC009398 Genomic DNA. Translation: AAF17665.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28643.1.
AF372893 mRNA. Translation: AAK49609.1. Different initiation.
AY052349 mRNA. Translation: AAK96541.1. Different initiation.
AY057722 mRNA. Translation: AAL15352.1.
PIRiB86241.
RefSeqiNP_563877.1. NM_100952.4.
UniGeneiAt.22136.

Genome annotation databases

EnsemblPlantsiAT1G10760.1; AT1G10760.1; AT1G10760.
GeneIDi837619.
GrameneiAT1G10760.1; AT1G10760.1; AT1G10760.
KEGGiath:AT1G10760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312027 mRNA. Translation: AAG47821.1.
AC007354 Genomic DNA. Translation: AAD31337.1. Sequence problems.
AC009398 Genomic DNA. Translation: AAF17665.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28643.1.
AF372893 mRNA. Translation: AAK49609.1. Different initiation.
AY052349 mRNA. Translation: AAK96541.1. Different initiation.
AY057722 mRNA. Translation: AAL15352.1.
PIRiB86241.
RefSeqiNP_563877.1. NM_100952.4.
UniGeneiAt.22136.

3D structure databases

ProteinModelPortaliQ9SAC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22859. 5 interactors.
IntActiQ9SAC6. 1 interactor.
MINTiMINT-8331304.
STRINGi3702.AT1G10760.1.

Protein family/group databases

CAZyiCBM45. Carbohydrate-Binding Module Family 45.

PTM databases

iPTMnetiQ9SAC6.

Proteomic databases

PaxDbiQ9SAC6.
PRIDEiQ9SAC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10760.1; AT1G10760.1; AT1G10760.
GeneIDi837619.
GrameneiAT1G10760.1; AT1G10760.1; AT1G10760.
KEGGiath:AT1G10760.

Organism-specific databases

TAIRiAT1G10760.

Phylogenomic databases

eggNOGiENOG410IHGM. Eukaryota.
ENOG410XUE8. LUCA.
HOGENOMiHOG000265165.
InParanoidiQ9SAC6.
KOiK08244.
OMAiCLKGWNQ.
OrthoDBiEOG093600AI.
PhylomeDBiQ9SAC6.

Enzyme and pathway databases

BioCyciARA:AT1G10760-MONOMER.
MetaCyc:AT1G10760-MONOMER.

Miscellaneous databases

PROiQ9SAC6.

Gene expression databases

GenevisibleiQ9SAC6. AT.

Family and domain databases

Gene3Di3.30.1490.20. 2 hits.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF01326. PPDK_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGWD1_ARATH
AccessioniPrimary (citable) accession number: Q9SAC6
Secondary accession number(s): Q93VD0
, Q940Z0, Q9FPP2, Q9SGX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the C-terminal domain, and the third partial reaction is catalyzed at an active site located on the N-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the C-terminal domain to that of the N-terminal domain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.