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Q9SAA9

- CP511_ARATH

UniProt

Q9SAA9 - CP511_ARATH

Protein

Sterol 14-demethylase

Gene

CYP51G1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.3 Publications

    Catalytic activityi

    A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.2 Publications

    Cofactori

    Heme group.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi433 – 4331Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. methyltransferase activity Source: UniProtKB-KW
    4. sterol 14-demethylase activity Source: TAIR

    GO - Biological processi

    1. demethylation Source: GOC
    2. sterol biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Methyltransferase, Monooxygenase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT1G11680-MONOMER.
    MetaCyc:AT1G11680-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sterol 14-demethylase (EC:1.14.13.70)
    Alternative name(s):
    Cytochrome P450 51A2
    Cytochrome P450 51G1
    Short name:
    AtCYP51
    Obtusifoliol 14-demethylase
    Protein EMBRYO DEFECTIVE 1738
    Gene namesi
    Name:CYP51G1
    Synonyms:CYP51A2, EMB1738
    Ordered Locus Names:At1g11680
    ORF Names:F25C20.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G11680.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. Golgi apparatus Source: TAIR
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Lack of membrane integrity. Seedling lethality.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Sterol 14-demethylasePRO_0000413010Add
    BLAST

    Proteomic databases

    PaxDbiQ9SAA9.
    PRIDEiQ9SAA9.

    Expressioni

    Tissue specificityi

    Expressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings.1 Publication

    Gene expression databases

    GenevestigatoriQ9SAA9.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G11680.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SAA9.
    SMRiQ9SAA9. Positions 40-485.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000042780.
    InParanoidiQ9SAA9.
    KOiK05917.
    OMAiMIHTPHN.
    PhylomeDBiQ9SAA9.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00465. EP450IV.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9SAA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELDSENKLL KTGLVIVATL VIAKLIFSFF TSDSKKKRLP PTLKAWPPLV    50
    GSLIKFLKGP IIMLREEYPK LGSVFTVNLV HKKITFLIGP EVSAHFFKAS 100
    ESDLSQQEVY QFNVPTFGPG VVFDVDYSVR QEQFRFFTEA LRVNKLKGYV 150
    DMMVTEAEDY FSKWGESGEV DIKVELERLI ILTASRCLLG REVRDQLFDD 200
    VSALFHDLDN GMLPISVLFP YLPIPAHRRR DRAREKLSEI FAKIIGSRKR 250
    SGKTENDMLQ CFIESKYKDG RQTTESEVTG LLIAALFAGQ HTSSITSTWT 300
    GAYLMRYKEY FSAALDEQKN LIAKHGDKID HDILSEMDVL YRCIKEALRL 350
    HPPLIMLMRA SHSDFSVTAR DGKTYDIPKG HIVATSPAFA NRLPHIFKDP 400
    DTYDPERFSP GREEDKAAGA FSYIAFGGGR HGCLGEPFAY LQIKAIWSHL 450
    LRNFELELVS PFPEIDWNAM VVGVKGNVMV RYKRRQLS 488
    Length:488
    Mass (Da):55,495
    Last modified:May 1, 2000 - v1
    Checksum:i6B68225BC13A4419
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014459 mRNA. Translation: BAB61873.1.
    AC007296 Genomic DNA. Translation: AAD30254.1.
    CP002684 Genomic DNA. Translation: AEE28769.1.
    AY050860 mRNA. Translation: AAK92797.1.
    AF410338 mRNA. Translation: AAK95324.1.
    AY091203 mRNA. Translation: AAM14142.1.
    AY084517 mRNA. Translation: AAM61085.1.
    PIRiD86250.
    RefSeqiNP_172633.1. NM_101040.3.
    UniGeneiAt.22780.

    Genome annotation databases

    EnsemblPlantsiAT1G11680.1; AT1G11680.1; AT1G11680.
    GeneIDi837712.
    KEGGiath:AT1G11680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014459 mRNA. Translation: BAB61873.1 .
    AC007296 Genomic DNA. Translation: AAD30254.1 .
    CP002684 Genomic DNA. Translation: AEE28769.1 .
    AY050860 mRNA. Translation: AAK92797.1 .
    AF410338 mRNA. Translation: AAK95324.1 .
    AY091203 mRNA. Translation: AAM14142.1 .
    AY084517 mRNA. Translation: AAM61085.1 .
    PIRi D86250.
    RefSeqi NP_172633.1. NM_101040.3.
    UniGenei At.22780.

    3D structure databases

    ProteinModelPortali Q9SAA9.
    SMRi Q9SAA9. Positions 40-485.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G11680.1-P.

    Proteomic databases

    PaxDbi Q9SAA9.
    PRIDEi Q9SAA9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G11680.1 ; AT1G11680.1 ; AT1G11680 .
    GeneIDi 837712.
    KEGGi ath:AT1G11680.

    Organism-specific databases

    GeneFarmi 1167. 94.
    TAIRi AT1G11680.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000042780.
    InParanoidi Q9SAA9.
    KOi K05917.
    OMAi MIHTPHN.
    PhylomeDBi Q9SAA9.

    Enzyme and pathway databases

    BioCyci ARA:AT1G11680-MONOMER.
    MetaCyc:AT1G11680-MONOMER.

    Miscellaneous databases

    PROi Q9SAA9.

    Gene expression databases

    Genevestigatori Q9SAA9.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00465. EP450IV.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Obtusifoliol 14alpha-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life."
      Kushiro M., Nakano T., Sato K., Yamagishi K., Asami T., Nakano A., Takatsuto S., Fujioka S., Ebizuka Y., Yoshida S.
      Biochem. Biophys. Res. Commun. 285:98-104(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
    7. "Arabidopsis cyp51 mutant shows postembryonic seedling lethality associated with lack of membrane integrity."
      Kim H.B., Schaller H., Goh C.H., Kwon M., Choe S., An C.S., Durst F., Feldmann K.A., Feyereisen R.
      Plant Physiol. 138:2033-2047(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    8. "Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity."
      O'Brien M., Chantha S.C., Rahier A., Matton D.P.
      Plant Physiol. 139:734-749(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCP511_ARATH
    AccessioniPrimary (citable) accession number: Q9SAA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Decreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14-alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3