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Q9SAA9

- CP511_ARATH

UniProt

Q9SAA9 - CP511_ARATH

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Protein
Sterol 14-demethylase
Gene
CYP51G1, CYP51A2, EMB1738, At1g11680, F25C20.17
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.3 Publications

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.2 Publications

Cofactori

Heme group.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi433 – 4331Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. methyltransferase activity Source: UniProtKB-KW
  4. sterol 14-demethylase activity Source: TAIR

GO - Biological processi

  1. demethylation Source: GOC
  2. sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Monooxygenase, Oxidoreductase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G11680-MONOMER.
MetaCyc:AT1G11680-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 14-demethylase (EC:1.14.13.70)
Alternative name(s):
Cytochrome P450 51A2
Cytochrome P450 51G1
Short name:
AtCYP51
Obtusifoliol 14-demethylase
Protein EMBRYO DEFECTIVE 1738
Gene namesi
Name:CYP51G1
Synonyms:CYP51A2, EMB1738
Ordered Locus Names:At1g11680
ORF Names:F25C20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G11680.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Lack of membrane integrity. Seedling lethality.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Sterol 14-demethylase
PRO_0000413010Add
BLAST

Proteomic databases

PaxDbiQ9SAA9.
PRIDEiQ9SAA9.

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings.1 Publication

Gene expression databases

GenevestigatoriQ9SAA9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G11680.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9SAA9.
SMRiQ9SAA9. Positions 40-485.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.
InParanoidiQ9SAA9.
KOiK05917.
OMAiMIHTPHN.
PhylomeDBiQ9SAA9.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9SAA9-1 [UniParc]FASTAAdd to Basket

« Hide

MELDSENKLL KTGLVIVATL VIAKLIFSFF TSDSKKKRLP PTLKAWPPLV    50
GSLIKFLKGP IIMLREEYPK LGSVFTVNLV HKKITFLIGP EVSAHFFKAS 100
ESDLSQQEVY QFNVPTFGPG VVFDVDYSVR QEQFRFFTEA LRVNKLKGYV 150
DMMVTEAEDY FSKWGESGEV DIKVELERLI ILTASRCLLG REVRDQLFDD 200
VSALFHDLDN GMLPISVLFP YLPIPAHRRR DRAREKLSEI FAKIIGSRKR 250
SGKTENDMLQ CFIESKYKDG RQTTESEVTG LLIAALFAGQ HTSSITSTWT 300
GAYLMRYKEY FSAALDEQKN LIAKHGDKID HDILSEMDVL YRCIKEALRL 350
HPPLIMLMRA SHSDFSVTAR DGKTYDIPKG HIVATSPAFA NRLPHIFKDP 400
DTYDPERFSP GREEDKAAGA FSYIAFGGGR HGCLGEPFAY LQIKAIWSHL 450
LRNFELELVS PFPEIDWNAM VVGVKGNVMV RYKRRQLS 488
Length:488
Mass (Da):55,495
Last modified:May 1, 2000 - v1
Checksum:i6B68225BC13A4419
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014459 mRNA. Translation: BAB61873.1.
AC007296 Genomic DNA. Translation: AAD30254.1.
CP002684 Genomic DNA. Translation: AEE28769.1.
AY050860 mRNA. Translation: AAK92797.1.
AF410338 mRNA. Translation: AAK95324.1.
AY091203 mRNA. Translation: AAM14142.1.
AY084517 mRNA. Translation: AAM61085.1.
PIRiD86250.
RefSeqiNP_172633.1. NM_101040.3.
UniGeneiAt.22780.

Genome annotation databases

EnsemblPlantsiAT1G11680.1; AT1G11680.1; AT1G11680.
GeneIDi837712.
KEGGiath:AT1G11680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014459 mRNA. Translation: BAB61873.1 .
AC007296 Genomic DNA. Translation: AAD30254.1 .
CP002684 Genomic DNA. Translation: AEE28769.1 .
AY050860 mRNA. Translation: AAK92797.1 .
AF410338 mRNA. Translation: AAK95324.1 .
AY091203 mRNA. Translation: AAM14142.1 .
AY084517 mRNA. Translation: AAM61085.1 .
PIRi D86250.
RefSeqi NP_172633.1. NM_101040.3.
UniGenei At.22780.

3D structure databases

ProteinModelPortali Q9SAA9.
SMRi Q9SAA9. Positions 40-485.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G11680.1-P.

Proteomic databases

PaxDbi Q9SAA9.
PRIDEi Q9SAA9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G11680.1 ; AT1G11680.1 ; AT1G11680 .
GeneIDi 837712.
KEGGi ath:AT1G11680.

Organism-specific databases

GeneFarmi 1167. 94.
TAIRi AT1G11680.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000042780.
InParanoidi Q9SAA9.
KOi K05917.
OMAi MIHTPHN.
PhylomeDBi Q9SAA9.

Enzyme and pathway databases

BioCyci ARA:AT1G11680-MONOMER.
MetaCyc:AT1G11680-MONOMER.

Miscellaneous databases

PROi Q9SAA9.

Gene expression databases

Genevestigatori Q9SAA9.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Obtusifoliol 14alpha-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life."
    Kushiro M., Nakano T., Sato K., Yamagishi K., Asami T., Nakano A., Takatsuto S., Fujioka S., Ebizuka Y., Yoshida S.
    Biochem. Biophys. Res. Commun. 285:98-104(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
  7. "Arabidopsis cyp51 mutant shows postembryonic seedling lethality associated with lack of membrane integrity."
    Kim H.B., Schaller H., Goh C.H., Kwon M., Choe S., An C.S., Durst F., Feldmann K.A., Feyereisen R.
    Plant Physiol. 138:2033-2047(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. "Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity."
    O'Brien M., Chantha S.C., Rahier A., Matton D.P.
    Plant Physiol. 139:734-749(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCP511_ARATH
AccessioniPrimary (citable) accession number: Q9SAA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Decreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14-alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi