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Protein

Sterol 14-demethylase

Gene

CYP51G1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.3 Publications

Miscellaneous

Decreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14-alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.2 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi433Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • methyltransferase activity Source: UniProtKB-KW
  • sterol 14-demethylase activity Source: TAIR

GO - Biological processi

  • sterol biosynthetic process Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Monooxygenase, Oxidoreductase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G11680-MONOMER
MetaCyc:AT1G11680-MONOMER
ReactomeiR-ATH-191273 Cholesterol biosynthesis
R-ATH-211976 Endogenous sterols

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 14-demethylase (EC:1.14.13.70)
Alternative name(s):
Cytochrome P450 51A2
Cytochrome P450 51G1
Short name:
AtCYP51
Obtusifoliol 14-demethylase
Protein EMBRYO DEFECTIVE 1738
Gene namesi
Name:CYP51G1
Synonyms:CYP51A2, EMB1738
Ordered Locus Names:At1g11680
ORF Names:F25C20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G11680
TAIRilocus:2027312 AT1G11680

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Lack of membrane integrity. Seedling lethality.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004130101 – 488Sterol 14-demethylaseAdd BLAST488

Proteomic databases

PaxDbiQ9SAA9

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings.1 Publication

Gene expression databases

ExpressionAtlasiQ9SAA9 baseline and differential
GenevisibleiQ9SAA9 AT

Interactioni

Protein-protein interaction databases

BioGridi22952, 1 interactor
STRINGi3702.AT1G11680.1

Structurei

3D structure databases

ProteinModelPortaliQ9SAA9
SMRiQ9SAA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
HOGENOMiHOG000042780
InParanoidiQ9SAA9
KOiK05917
OMAiAPIHSIM
OrthoDBiEOG09360A5D
PhylomeDBiQ9SAA9

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SAA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDSENKLL KTGLVIVATL VIAKLIFSFF TSDSKKKRLP PTLKAWPPLV
60 70 80 90 100
GSLIKFLKGP IIMLREEYPK LGSVFTVNLV HKKITFLIGP EVSAHFFKAS
110 120 130 140 150
ESDLSQQEVY QFNVPTFGPG VVFDVDYSVR QEQFRFFTEA LRVNKLKGYV
160 170 180 190 200
DMMVTEAEDY FSKWGESGEV DIKVELERLI ILTASRCLLG REVRDQLFDD
210 220 230 240 250
VSALFHDLDN GMLPISVLFP YLPIPAHRRR DRAREKLSEI FAKIIGSRKR
260 270 280 290 300
SGKTENDMLQ CFIESKYKDG RQTTESEVTG LLIAALFAGQ HTSSITSTWT
310 320 330 340 350
GAYLMRYKEY FSAALDEQKN LIAKHGDKID HDILSEMDVL YRCIKEALRL
360 370 380 390 400
HPPLIMLMRA SHSDFSVTAR DGKTYDIPKG HIVATSPAFA NRLPHIFKDP
410 420 430 440 450
DTYDPERFSP GREEDKAAGA FSYIAFGGGR HGCLGEPFAY LQIKAIWSHL
460 470 480
LRNFELELVS PFPEIDWNAM VVGVKGNVMV RYKRRQLS
Length:488
Mass (Da):55,495
Last modified:May 1, 2000 - v1
Checksum:i6B68225BC13A4419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014459 mRNA Translation: BAB61873.1
AC007296 Genomic DNA Translation: AAD30254.1
CP002684 Genomic DNA Translation: AEE28769.1
AY050860 mRNA Translation: AAK92797.1
AF410338 mRNA Translation: AAK95324.1
AY091203 mRNA Translation: AAM14142.1
AY084517 mRNA Translation: AAM61085.1
PIRiD86250
RefSeqiNP_172633.1, NM_101040.4
UniGeneiAt.22780

Genome annotation databases

EnsemblPlantsiAT1G11680.1; AT1G11680.1; AT1G11680
GeneIDi837712
GrameneiAT1G11680.1; AT1G11680.1; AT1G11680
KEGGiath:AT1G11680

Similar proteinsi

Entry informationi

Entry nameiCP511_ARATH
AccessioniPrimary (citable) accession number: Q9SAA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health