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Protein

Sterol 14-demethylase

Gene

CYP51G1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.3 Publications

Miscellaneous

Decreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14-alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.2 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi433Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • methyltransferase activity Source: UniProtKB-KW
  • sterol 14-demethylase activity Source: TAIR

GO - Biological processi

  • sterol biosynthetic process Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Monooxygenase, Oxidoreductase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G11680-MONOMER.
MetaCyc:AT1G11680-MONOMER.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.
R-ATH-211976. Endogenous sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 14-demethylase (EC:1.14.13.70)
Alternative name(s):
Cytochrome P450 51A2
Cytochrome P450 51G1
Short name:
AtCYP51
Obtusifoliol 14-demethylase
Protein EMBRYO DEFECTIVE 1738
Gene namesi
Name:CYP51G1
Synonyms:CYP51A2, EMB1738
Ordered Locus Names:At1g11680
ORF Names:F25C20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G11680.
TAIRilocus:2027312. AT1G11680.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Lack of membrane integrity. Seedling lethality.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004130101 – 488Sterol 14-demethylaseAdd BLAST488

Proteomic databases

PaxDbiQ9SAA9.

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings.1 Publication

Gene expression databases

ExpressionAtlasiQ9SAA9. baseline and differential.
GenevisibleiQ9SAA9. AT.

Interactioni

Protein-protein interaction databases

BioGridi22952. 1 interactor.
STRINGi3702.AT1G11680.1.

Structurei

3D structure databases

ProteinModelPortaliQ9SAA9.
SMRiQ9SAA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000042780.
InParanoidiQ9SAA9.
KOiK05917.
OMAiAPIHSIM.
OrthoDBiEOG09360A5D.
PhylomeDBiQ9SAA9.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9SAA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDSENKLL KTGLVIVATL VIAKLIFSFF TSDSKKKRLP PTLKAWPPLV
60 70 80 90 100
GSLIKFLKGP IIMLREEYPK LGSVFTVNLV HKKITFLIGP EVSAHFFKAS
110 120 130 140 150
ESDLSQQEVY QFNVPTFGPG VVFDVDYSVR QEQFRFFTEA LRVNKLKGYV
160 170 180 190 200
DMMVTEAEDY FSKWGESGEV DIKVELERLI ILTASRCLLG REVRDQLFDD
210 220 230 240 250
VSALFHDLDN GMLPISVLFP YLPIPAHRRR DRAREKLSEI FAKIIGSRKR
260 270 280 290 300
SGKTENDMLQ CFIESKYKDG RQTTESEVTG LLIAALFAGQ HTSSITSTWT
310 320 330 340 350
GAYLMRYKEY FSAALDEQKN LIAKHGDKID HDILSEMDVL YRCIKEALRL
360 370 380 390 400
HPPLIMLMRA SHSDFSVTAR DGKTYDIPKG HIVATSPAFA NRLPHIFKDP
410 420 430 440 450
DTYDPERFSP GREEDKAAGA FSYIAFGGGR HGCLGEPFAY LQIKAIWSHL
460 470 480
LRNFELELVS PFPEIDWNAM VVGVKGNVMV RYKRRQLS
Length:488
Mass (Da):55,495
Last modified:May 1, 2000 - v1
Checksum:i6B68225BC13A4419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014459 mRNA. Translation: BAB61873.1.
AC007296 Genomic DNA. Translation: AAD30254.1.
CP002684 Genomic DNA. Translation: AEE28769.1.
AY050860 mRNA. Translation: AAK92797.1.
AF410338 mRNA. Translation: AAK95324.1.
AY091203 mRNA. Translation: AAM14142.1.
AY084517 mRNA. Translation: AAM61085.1.
PIRiD86250.
RefSeqiNP_172633.1. NM_101040.4.
UniGeneiAt.22780.

Genome annotation databases

EnsemblPlantsiAT1G11680.1; AT1G11680.1; AT1G11680.
GeneIDi837712.
GrameneiAT1G11680.1; AT1G11680.1; AT1G11680.
KEGGiath:AT1G11680.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCP511_ARATH
AccessioniPrimary (citable) accession number: Q9SAA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 2000
Last modified: June 7, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families