Q9SAA9 (CP511_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 14-demethylase
EC=1.14.13.70

Alternative name(s):
Cytochrome P450 51A2
Cytochrome P450 51G1
Short name=AtCYP51
Obtusifoliol 14-demethylase
Protein EMBRYO DEFECTIVE 1738

Gene names

Name:	CYP51G1
Synonyms:	CYP51A2, EMB1738
Ordered Locus Names:	At1g11680
ORF Names:	F25C20.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol. Ref.1 Ref.7 Ref.8
Catalytic activity	A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O. Ref.1 Ref.7
Cofactor	Heme group.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings. Ref.7
Disruption phenotype	Lack of membrane integrity. Seedling lethality. Ref.6 Ref.7 Ref.8
Miscellaneous	Decreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14-alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Methyltransferase Monooxygenase Oxidoreductase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sterol biosynthetic process Inferred from mutant phenotype Ref.8. Source: TAIR
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW sterol 14-demethylase activity Traceable author statement Ref.8. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Sterol 14-demethylase		PRO_0000413010
Regions
	Transmembrane	12 – 32	21	Helical; Potential
Sites
	Metal binding	433	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9SAA9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6B68225BC13A4419	FASTA	488	55,495
	10 20 30 40 50 60 MELDSENKLL KTGLVIVATL VIAKLIFSFF TSDSKKKRLP PTLKAWPPLV GSLIKFLKGP 70 80 90 100 110 120 IIMLREEYPK LGSVFTVNLV HKKITFLIGP EVSAHFFKAS ESDLSQQEVY QFNVPTFGPG 130 140 150 160 170 180 VVFDVDYSVR QEQFRFFTEA LRVNKLKGYV DMMVTEAEDY FSKWGESGEV DIKVELERLI 190 200 210 220 230 240 ILTASRCLLG REVRDQLFDD VSALFHDLDN GMLPISVLFP YLPIPAHRRR DRAREKLSEI 250 260 270 280 290 300 FAKIIGSRKR SGKTENDMLQ CFIESKYKDG RQTTESEVTG LLIAALFAGQ HTSSITSTWT 310 320 330 340 350 360 GAYLMRYKEY FSAALDEQKN LIAKHGDKID HDILSEMDVL YRCIKEALRL HPPLIMLMRA 370 380 390 400 410 420 SHSDFSVTAR DGKTYDIPKG HIVATSPAFA NRLPHIFKDP DTYDPERFSP GREEDKAAGA 430 440 450 460 470 480 FSYIAFGGGR HGCLGEPFAY LQIKAIWSHL LRNFELELVS PFPEIDWNAM VVGVKGNVMV RYKRRQLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Obtusifoliol 14alpha-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life." Kushiro M., Nakano T., Sato K., Yamagishi K., Asami T., Nakano A., Takatsuto S., Fujioka S., Ebizuka Y., Yoshida S. Biochem. Biophys. Res. Commun. 285:98-104(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of genes required for embryo development in Arabidopsis." Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S., Sweeney T.C., McElver J., Aux G., Patton D., Meinke D. Plant Physiol. 135:1206-1220(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
[7]	"Arabidopsis cyp51 mutant shows postembryonic seedling lethality associated with lack of membrane integrity." Kim H.B., Schaller H., Goh C.H., Kwon M., Choe S., An C.S., Durst F., Feldmann K.A., Feyereisen R. Plant Physiol. 138:2033-2047(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[8]	"Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity." O'Brien M., Chantha S.C., Rahier A., Matton D.P. Plant Physiol. 139:734-749(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB014459 mRNA. Translation: BAB61873.1. AC007296 Genomic DNA. Translation: AAD30254.1. CP002684 Genomic DNA. Translation: AEE28769.1. AY050860 mRNA. Translation: AAK92797.1. AF410338 mRNA. Translation: AAK95324.1. AY091203 mRNA. Translation: AAM14142.1. AY084517 mRNA. Translation: AAM61085.1.
IPI	IPI00548435.
PIR	D86250.
RefSeq	NP_172633.1. NM_101040.3.
UniGene	At.22780.
3D structure databases
ProteinModelPortal	Q9SAA9.
SMR	Q9SAA9. Positions 40-485.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G11680.1-P.
Proteomic databases
PaxDb	Q9SAA9.
PRIDE	Q9SAA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G11680.1; AT1G11680.1; AT1G11680.
GeneID	837712.
KEGG	ath:AT1G11680.
Organism-specific databases
GeneFarm	1167. 94.
TAIR	At1g11680.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000042780.
InParanoid	Q9SAA9.
KO	K05917.
OMA	WGDEGEI.
PhylomeDB	Q9SAA9.
ProtClustDB	CLSN2682182.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G11680-MONOMER.
Gene expression databases
Genevestigator	Q9SAA9.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP511_ARATH

Accession

Primary (citable) accession number: Q9SAA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program