Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inosine-5'-monophosphate dehydrogenase 2

Gene

At1g16350

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi: XMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-5'-monophosphate dehydrogenase, Inosine-5'-monophosphate dehydrogenase 2 (At1g16350), Inosine-5'-monophosphate dehydrogenase (AXX17_At1g17130), Inosine-5'-monophosphate dehydrogenase 1 (IMPDH), Inosine-5'-monophosphate dehydrogenase (AXX17_At1g74260)
This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi316Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi318Potassium; via carbonyl oxygenUniRule annotation1
Binding sitei319IMPUniRule annotation1
Active sitei321Thioimidate intermediateUniRule annotation1
Metal bindingi321Potassium; via carbonyl oxygenUniRule annotation1
Active sitei417Proton acceptorUniRule annotation1
Binding sitei429IMPUniRule annotation1
Metal bindingi488Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi489Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
Metal bindingi490Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi264 – 266NADUniRule annotation3
Nucleotide bindingi314 – 316NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

  • GMP biosynthetic process Source: UniProtKB-KW
  • GTP biosynthetic process Source: GO_Central
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processGMP biosynthesis, Purine biosynthesis
LigandMetal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciARA:AT1G16350-MONOMER
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00601; UER00295

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase 2UniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenase 2UniRule annotation
Short name:
IMPD 2UniRule annotation
Short name:
IMPDH 2UniRule annotation
Gene namesi
Ordered Locus Names:At1g16350
ORF Names:F3O9.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G16350
TAIRilocus:2032775 AT1G16350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000936872 – 502Inosine-5'-monophosphate dehydrogenase 2Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9SA34
PRIDEiQ9SA34

PTM databases

iPTMnetiQ9SA34

Expressioni

Gene expression databases

ExpressionAtlasiQ9SA34 baseline and differential
GenevisibleiQ9SA34 AT

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi23444, 1 interactor
STRINGi3702.AT1G16350.1

Structurei

3D structure databases

ProteinModelPortaliQ9SA34
SMRiQ9SA34
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 225CBSUniRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni354 – 356IMP bindingUniRule annotation3
Regioni377 – 378IMP bindingUniRule annotation2
Regioni401 – 405IMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation

Keywords - Domaini

CBS domain

Phylogenomic databases

eggNOGiKOG2550 Eukaryota
COG0516 LUCA
HOGENOMiHOG000165752
KOiK00088
OMAiNLGSQNR
OrthoDBiEOG093607LZ
PhylomeDBiQ9SA34

Family and domain databases

CDDicd00381 IMPDH, 1 hit
HAMAPiMF_01964 IMPDH, 1 hit
InterProiView protein in InterPro
IPR005990 IMP_DH
IPR015875 IMP_DH/GMP_Rdtase_CS
IPR001093 IMP_DH_GMPRt
PfamiView protein in Pfam
PF00478 IMPDH, 1 hit
PIRSFiPIRSF000130 IMPDH, 1 hit
TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
PROSITEiView protein in PROSITE
PS00487 IMP_DH_GMP_RED, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SA34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFEDGFSA EKLFSQGYSY TYDDVIFLPH FIDFSTDAVS LSTRLSKRVP
60 70 80 90 100
LSIPCVASPM DTVSESHMAA AMAALGGIGI VHYNCDIDTQ ASVIRHAKSL
110 120 130 140 150
QVPIASDAVF KCPEHQIGSV DDFGPSSFVF VSQTGTLTPK LLGYVSKSEW
160 170 180 190 200
SSMKDDQKEV KIYDYMKSCE NKDYYVPWDI DLDKIEAVLE DKQKGFVVLE
210 220 230 240 250
KEGETVNVVT KDDVERVKGY PKLGSGTVGA DKKWMVGAAI GTRESDKERL
260 270 280 290 300
EHLVKAGANV VVLDSSQGNS IYQLEMIKYV KNTYPELDVV GGNVVTMYQA
310 320 330 340 350
ENLIKAGVDG LRVGMGSGSI CTTQEVCAVG RGQATAVYKV STLAAQHGVP
360 370 380 390 400
VIADGGISNS GHIVKALVLG ASTVMMGSFL AGSTEAPGAY EYRNGRRVKK
410 420 430 440 450
YRGMGSLEAM TKGSDQRYLG DTAKLKIAQG VVGAVADKGS VLKFIPYTMH
460 470 480 490 500
AVKQGFQDLG ASSLQSAHEL LRDNTLRLEA RTGAAQIEGG IHGLVSYEKK

SF
Length:502
Mass (Da):54,051
Last modified:May 1, 2000 - v1
Checksum:iFB87D84160818310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006341 Genomic DNA Translation: AAD34687.1
CP002684 Genomic DNA Translation: AEE29441.1
PIRiF86298
RefSeqiNP_173085.1, NM_101501.3
UniGeneiAt.41887

Genome annotation databases

EnsemblPlantsiAT1G16350.1; AT1G16350.1; AT1G16350
GeneIDi838204
GrameneiAT1G16350.1; AT1G16350.1; AT1G16350
KEGGiath:AT1G16350

Similar proteinsi

Entry informationi

Entry nameiIMDH2_ARATH
AccessioniPrimary (citable) accession number: Q9SA34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health