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Q9S9U0 (CALSB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 11
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 1
Gene names
Name:CALS11
Synonyms:GSL1
Ordered Locus Names:At4g04970
ORF Names:T32N4.8
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1768 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required the formation of the callose wall separating the tetraspores (interstitial wall), but not for the callose wall surrounding the pollen mother cells (peripheral wall). Functionally redudant to CALS12 (GSL5). During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.5

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Ubiquitous. Ref.5

Disruption phenotype

Plants develop collapsed and inviable pollen grains. Ref.5

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17681768Callose synthase 11
PRO_0000334583

Regions

Topological domain1 – 308308Cytoplasmic Potential
Transmembrane309 – 32921Helical; Potential
Topological domain330 – 3356Extracellular Potential
Transmembrane336 – 35621Helical; Potential
Topological domain357 – 37014Cytoplasmic Potential
Transmembrane371 – 39121Helical; Potential
Topological domain392 – 42130Extracellular Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 48038Cytoplasmic Potential
Transmembrane481 – 50121Helical; Potential
Topological domain502 – 53029Extracellular Potential
Transmembrane531 – 55121Helical; Potential
Topological domain552 – 1341790Cytoplasmic Potential
Transmembrane1342 – 136221Helical; Potential
Topological domain1363 – 138624Extracellular Potential
Transmembrane1387 – 140721Helical; Potential
Topological domain1408 – 14136Cytoplasmic Potential
Transmembrane1414 – 143421Helical; Potential
Topological domain1435 – 148147Extracellular Potential
Transmembrane1482 – 150221Helical; Potential
Topological domain1503 – 15086Cytoplasmic Potential
Transmembrane1509 – 152921Helical; Potential
Topological domain1530 – 158354Extracellular Potential
Transmembrane1584 – 160421Helical; Potential
Topological domain1605 – 16128Cytoplasmic Potential
Transmembrane1613 – 163321Helical; Potential
Topological domain1634 – 164916Extracellular Potential
Transmembrane1650 – 167021Helical; Potential
Topological domain1671 – 16733Cytoplasmic Potential
Transmembrane1674 – 169421Helical; Potential
Topological domain1695 – 171925Extracellular Potential
Transmembrane1720 – 174021Helical; Potential
Topological domain1741 – 176828Cytoplasmic Potential
Compositional bias42 – 465Poly-Ala
Compositional bias311 – 3166Poly-Leu
Compositional bias1593 – 15964Poly-Phe

Sequences

Sequence LengthMass (Da)Tools
Q9S9U0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 1F7C5E0CF74EFF0C

FASTA1,768205,526
        10         20         30         40         50         60 
MRRQRPSVAT ARDAPSLEVY NIIPIHDFLT EHPSLRYPEV RAAAAALRIV GDLPKPPFAD 

        70         80         90        100        110        120 
FTPRMDLMDW LGLLFGFQID NVRNQRENLV LHLANSQMRL QPPPRHPDGL DPTVLRRFRK 

       130        140        150        160        170        180 
KLLRNYTNWC SFLGVRCHVT SPIQSRHQTN AVLNLRRELL YVALYLLIWG ESANLRFMPE 

       190        200        210        220        230        240 
CLCYIFHHMA MELNKVLAGE FDDMTGMPYW PSFSGDCAFL KSVVMPIYKT VKTEVESSNN 

       250        260        270        280        290        300 
GTKPHSAWRN YDDINEYFWS KRALKSLKWP LDYTSNFFDT TPKSSRVGKT GFVEQRSFWN 

       310        320        330        340        350        360 
VYRSFDRLWI LLLLYLQAAI IVATSDVKFP WQDRDVEVAL LTVFISWAGL RLLQSVLDAS 

       370        380        390        400        410        420 
TQYSLVSRET YWLFIRLTLK FVVAVAWTVL FSVFYARIWS QKNKDGVWSR AANERVVTFL 

       430        440        450        460        470        480 
KVVFVYVIPE LLALVLFIVP CIRNWVEELN LGVVYFLTWW FYSKTFVGRG MREGLVDNVK 

       490        500        510        520        530        540 
YTLFWIIVLA TKFIFSYFLQ IRPLIAPTRA LLNLKDATYN WHEFFGSTHR IAVGMLWLPV 

       550        560        570        580        590        600 
ILVYLMDLQI WYSIYSSLVG ATIGLFSHLG EIRNIDQLRL RFQFFSSAMQ FNLKPEEHLL 

       610        620        630        640        650        660 
SPKATMLKKA RDAIHRLKLR YGIGQPFNKI ESSQVEATWF ALIWNEIILT FREEDLISDR 

       670        680        690        700        710        720 
EVELLELPPN CWNIRVIRWP CFLLCNELLL ALSQANELCD APDHWLWSKI CSSEYRRCAV 

       730        740        750        760        770        780 
MEAFDSIKFV ILKIVKNGTE EESILNRLFM EIDENVENEK ITEVYKLTVL LRIHEKLISL 

       790        800        810        820        830        840 
LERLMDPEKK VFRIVNILQA LYELCAWEFP KTRRSTPQLR QLGLAPISLE ADTELLFVNA 

       850        860        870        880        890        900 
INLPPLDDVV FYRQIRRVHT ILTSRDPMHN VPKNIEARER LAFFSNSLFM TMPQAPSVEK 

       910        920        930        940        950        960 
MMAFSVLTPY YDEEVMYRQE MLRAENEDGI STLFYLQRIY EDEWVNFLER MRREGAENEN 

       970        980        990       1000       1010       1020 
DIWSKKVRDL RLWASYRGQT LSRTVRGMMY YYSALKKLAF LDSASEMDIR MGTQIAPEAR 

      1030       1040       1050       1060       1070       1080 
RSYYTNDGGD NTLQPTPSQE ISRMASGITH LLKGSEYGSA MMKFTYVVAC QVYGQHKARG 

      1090       1100       1110       1120       1130       1140 
DHRAEEILFL MKNHDALRIA YVDEVDLGRG EVEYYSVLVK FDQQLQREVE IYRIRLPGPL 

      1150       1160       1170       1180       1190       1200 
KLGEGKPENQ NHALIFTRGD AIQTIDMNQD NHFEEALKMR NLLESFKTYY GIRKPTILGV 

      1210       1220       1230       1240       1250       1260 
REKVFTGSVS SLAWFMSAQE TSFVTLGQRV LANPLKVRMH YGHPDVFDRF WFVPRGGISK 

      1270       1280       1290       1300       1310       1320 
ASRVINISED IFAGFNCTLR GGNVTHHEYI QVGKGRDVGL NQISMFEAKV ASGNGEQALS 

      1330       1340       1350       1360       1370       1380 
RDVYRLGHRL DFFRMLSFFY TTVGYYFNTM LIVFTVYAFL WGRLYLALSG VEKIAKDRSS 

      1390       1400       1410       1420       1430       1440 
SNEALGAILN QQFIIQLGLF TALPMILENS LERGFLPAVW DFITMQLQLA SFFYTFSMGT 

      1450       1460       1470       1480       1490       1500 
RTHYFGRTIL HGGAKYRATG RGFVVEHKKF AENYRLYART HFIKAIELAI ILLVYAAYSP 

      1510       1520       1530       1540       1550       1560 
LAKSSFVYIL MTISSWFLIT SWIISPFLFN PSGFDWLKTV NDFDDFIAWL WSRGGLFTKA 

      1570       1580       1590       1600       1610       1620 
DQSWFTWWNE EQEHLKTTGV WGKLLEIILD LRFFFFQYSI VYHLRIAENR TSIGVYLISW 

      1630       1640       1650       1660       1670       1680 
GCIIGIVAIY ITTIYAQKRY SVKEHIKYRF IQFLVILLTV LVVVMMLQFT KLTVVDLLIS 

      1690       1700       1710       1720       1730       1740 
LLAFVPTGWG LISIAQVLKP FLLSTVVWDT VISVARFYDL FFGLIVMAPV ALLSWLPGFQ 

      1750       1760 
NMQTRILFNE AFSRGLQISI ILAGKKST

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1361-1768.
Strain: cv. Columbia.
[4]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[5]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, NOMENCLATURE, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF162444 Genomic DNA. Translation: AAD48971.1.
AL161502 Genomic DNA. Translation: CAB81039.1.
CP002687 Genomic DNA. Translation: AEE82451.1.
AY050990 mRNA. Translation: AAK93667.2.
IPIIPI00530909.
PIRE85062.
RefSeqNP_567278.1. NM_116736.1.
UniGeneAt.26533.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9S9U0.

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9S9U0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G04970.1; AT4G04970.1; AT4G04970.
GeneID825838.
GenomeReviewsGene locus AT4G04970 in contig CT486007_GR.
KEGGath:AT4G04970.
NMPDRfig|3702.1.peg.18310.

Organism-specific databases

TAIRAt4g04970.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
InParanoidQ9S9U0.
OMAYNWHEFF.
PhylomeDBQ9S9U0.
ProtClustDBCLSN2685951.

Gene expression databases

GenevestigatorQ9S9U0.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
KOK11000.
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALSB_ARATH
AccessionPrimary (citable) accession number: Q9S9U0
Secondary accession number(s): Q949N5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2000
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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