Reviewed, UniProtKB/Swiss-Prot Q9S9U0 (CALSB_ARATH)

Names and origin

Protein names

Recommended name:
    Callose synthase 11
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 1

Gene names

Name:	CALS11
Synonyms:	GSL1
Ordered Locus Names:	At4g04970
ORF Names:	T32N4.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1768 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required the formation of the callose wall separating the tetraspores (interstitial wall), but not for the callose wall surrounding the pollen mother cells (peripheral wall). Functionally redudant to CALS12 (GSL5). During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.4
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Ubiquitous. Ref.4
Disruption phenotype	Plants develop collapsed and inviable pollen grains. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 48 family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	1,3-beta-glucan synthase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1768	1768	Callose synthase 11		PRO_0000334583
Regions
	Topological domain	1 – 308	308	Cytoplasmic Potential
	Transmembrane	309 – 329	21	Potential
	Topological domain	330 – 335	6	Extracellular Potential
	Transmembrane	336 – 356	21	Potential
	Topological domain	357 – 370	14	Cytoplasmic Potential
	Transmembrane	371 – 391	21	Potential
	Topological domain	392 – 421	30	Extracellular Potential
	Transmembrane	422 – 442	21	Potential
	Topological domain	443 – 480	38	Cytoplasmic Potential
	Transmembrane	481 – 501	21	Potential
	Topological domain	502 – 530	29	Extracellular Potential
	Transmembrane	531 – 551	21	Potential
	Topological domain	552 – 1341	790	Cytoplasmic Potential
	Transmembrane	1342 – 1362	21	Potential
	Topological domain	1363 – 1386	24	Extracellular Potential
	Transmembrane	1387 – 1407	21	Potential
	Topological domain	1408 – 1413	6	Cytoplasmic Potential
	Transmembrane	1414 – 1434	21	Potential
	Topological domain	1435 – 1481	47	Extracellular Potential
	Transmembrane	1482 – 1502	21	Potential
	Topological domain	1503 – 1508	6	Cytoplasmic Potential
	Transmembrane	1509 – 1529	21	Potential
	Topological domain	1530 – 1583	54	Extracellular Potential
	Transmembrane	1584 – 1604	21	Potential
	Topological domain	1605 – 1612	8	Cytoplasmic Potential
	Transmembrane	1613 – 1633	21	Potential
	Topological domain	1634 – 1649	16	Extracellular Potential
	Transmembrane	1650 – 1670	21	Potential
	Topological domain	1671 – 1673	3	Cytoplasmic Potential
	Transmembrane	1674 – 1694	21	Potential
	Topological domain	1695 – 1719	25	Extracellular Potential
	Transmembrane	1720 – 1740	21	Potential
	Topological domain	1741 – 1768	28	Cytoplasmic Potential
	Compositional bias	42 – 46	5	Poly-Ala
	Compositional bias	311 – 316	6	Poly-Leu
	Compositional bias	1593 – 1596	4	Poly-Phe

Sequences

	Sequence		Length	Mass (Da)
	Q9S9U0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1F7C5E0CF74EFF0C	FASTA	1,768	205,526
	10 20 30 40 50 60 MRRQRPSVAT ARDAPSLEVY NIIPIHDFLT EHPSLRYPEV RAAAAALRIV GDLPKPPFAD 70 80 90 100 110 120 FTPRMDLMDW LGLLFGFQID NVRNQRENLV LHLANSQMRL QPPPRHPDGL DPTVLRRFRK 130 140 150 160 170 180 KLLRNYTNWC SFLGVRCHVT SPIQSRHQTN AVLNLRRELL YVALYLLIWG ESANLRFMPE 190 200 210 220 230 240 CLCYIFHHMA MELNKVLAGE FDDMTGMPYW PSFSGDCAFL KSVVMPIYKT VKTEVESSNN 250 260 270 280 290 300 GTKPHSAWRN YDDINEYFWS KRALKSLKWP LDYTSNFFDT TPKSSRVGKT GFVEQRSFWN 310 320 330 340 350 360 VYRSFDRLWI LLLLYLQAAI IVATSDVKFP WQDRDVEVAL LTVFISWAGL RLLQSVLDAS 370 380 390 400 410 420 TQYSLVSRET YWLFIRLTLK FVVAVAWTVL FSVFYARIWS QKNKDGVWSR AANERVVTFL 430 440 450 460 470 480 KVVFVYVIPE LLALVLFIVP CIRNWVEELN LGVVYFLTWW FYSKTFVGRG MREGLVDNVK 490 500 510 520 530 540 YTLFWIIVLA TKFIFSYFLQ IRPLIAPTRA LLNLKDATYN WHEFFGSTHR IAVGMLWLPV 550 560 570 580 590 600 ILVYLMDLQI WYSIYSSLVG ATIGLFSHLG EIRNIDQLRL RFQFFSSAMQ FNLKPEEHLL 610 620 630 640 650 660 SPKATMLKKA RDAIHRLKLR YGIGQPFNKI ESSQVEATWF ALIWNEIILT FREEDLISDR 670 680 690 700 710 720 EVELLELPPN CWNIRVIRWP CFLLCNELLL ALSQANELCD APDHWLWSKI CSSEYRRCAV 730 740 750 760 770 780 MEAFDSIKFV ILKIVKNGTE EESILNRLFM EIDENVENEK ITEVYKLTVL LRIHEKLISL 790 800 810 820 830 840 LERLMDPEKK VFRIVNILQA LYELCAWEFP KTRRSTPQLR QLGLAPISLE ADTELLFVNA 850 860 870 880 890 900 INLPPLDDVV FYRQIRRVHT ILTSRDPMHN VPKNIEARER LAFFSNSLFM TMPQAPSVEK 910 920 930 940 950 960 MMAFSVLTPY YDEEVMYRQE MLRAENEDGI STLFYLQRIY EDEWVNFLER MRREGAENEN 970 980 990 1000 1010 1020 DIWSKKVRDL RLWASYRGQT LSRTVRGMMY YYSALKKLAF LDSASEMDIR MGTQIAPEAR 1030 1040 1050 1060 1070 1080 RSYYTNDGGD NTLQPTPSQE ISRMASGITH LLKGSEYGSA MMKFTYVVAC QVYGQHKARG 1090 1100 1110 1120 1130 1140 DHRAEEILFL MKNHDALRIA YVDEVDLGRG EVEYYSVLVK FDQQLQREVE IYRIRLPGPL 1150 1160 1170 1180 1190 1200 KLGEGKPENQ NHALIFTRGD AIQTIDMNQD NHFEEALKMR NLLESFKTYY GIRKPTILGV 1210 1220 1230 1240 1250 1260 REKVFTGSVS SLAWFMSAQE TSFVTLGQRV LANPLKVRMH YGHPDVFDRF WFVPRGGISK 1270 1280 1290 1300 1310 1320 ASRVINISED IFAGFNCTLR GGNVTHHEYI QVGKGRDVGL NQISMFEAKV ASGNGEQALS 1330 1340 1350 1360 1370 1380 RDVYRLGHRL DFFRMLSFFY TTVGYYFNTM LIVFTVYAFL WGRLYLALSG VEKIAKDRSS 1390 1400 1410 1420 1430 1440 SNEALGAILN QQFIIQLGLF TALPMILENS LERGFLPAVW DFITMQLQLA SFFYTFSMGT 1450 1460 1470 1480 1490 1500 RTHYFGRTIL HGGAKYRATG RGFVVEHKKF AENYRLYART HFIKAIELAI ILLVYAAYSP 1510 1520 1530 1540 1550 1560 LAKSSFVYIL MTISSWFLIT SWIISPFLFN PSGFDWLKTV NDFDDFIAWL WSRGGLFTKA 1570 1580 1590 1600 1610 1620 DQSWFTWWNE EQEHLKTTGV WGKLLEIILD LRFFFFQYSI VYHLRIAENR TSIGVYLISW 1630 1640 1650 1660 1670 1680 GCIIGIVAIY ITTIYAQKRY SVKEHIKYRF IQFLVILLTV LVVVMMLQFT KLTVVDLLIS 1690 1700 1710 1720 1730 1740 LLAFVPTGWG LISIAQVLKP FLLSTVVWDT VISVARFYDL FFGLIVMAPV ALLSWLPGFQ 1750 1760 NMQTRILFNE AFSRGLQISI ILAGKKST

References

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[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1361-1768. Strain: cv. Columbia.
[3]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: GENE FAMILY AND NOMENCLATURE.
[4]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, NOMENCLATURE, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF162444 Genomic DNA. Translation: AAD48971.1. AL161502 Genomic DNA. Translation: CAB81039.1. AY050990 mRNA. Translation: AAK93667.2.
IPI	IPI00530909.
PIR	E85062.
RefSeq	NP_567278.1.
UniGene	At.26533
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Proteomic databases
PRIDE	Q9S9U0.
Genome annotation databases
GeneID	825838.
GenomeReviews	Gene locus AT4G04970 in contig CT486007_GR.
KEGG	ath:AT4G04970.
Organism-specific databases
TAIR	At4g04970.
Phylogenomic databases
OMA	Q9S9U0. SEMDIRM.
Family and domain databases
InterPro	IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF02364. Glucan_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALSB_ARATH

Accession

Primary (citable) accession number: Q9S9U0
Secondary accession number(s): Q949N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)