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Protein

Vacuolar protein sorting-associated protein 28 homolog 2

Gene

VPS28-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28 homolog 2
Gene namesi
Name:VPS28-2
Ordered Locus Names:At4g05000
ORF Names:T32N4.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G05000.

Subcellular locationi

GO - Cellular componenti

  • ESCRT I complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Vacuolar protein sorting-associated protein 28 homolog 2PRO_0000120955Add
BLAST

Proteomic databases

PaxDbiQ9S9T7.
PRIDEiQ9S9T7.

Expressioni

Gene expression databases

GenevisibleiQ9S9T7. AT.

Interactioni

Subunit structurei

Component of the endosomal sorting required for transport complex I (ESCRT-I), composed of ELC, VPS28 and VPS37. Interacts with ELC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS37-1Q9SCP93EBI-3865335,EBI-3865264

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11153. 7 interactions.
IntActiQ9S9T7. 3 interactions.
STRINGi3702.AT4G05000.1.

Structurei

3D structure databases

ProteinModelPortaliQ9S9T7.
SMRiQ9S9T7. Positions 3-100, 115-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9999VPS28 N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini109 – 20597VPS28 C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation
Contains 1 VPS28 C-terminal domain.PROSITE-ProRule annotation
Contains 1 VPS28 N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3284. Eukaryota.
ENOG4111IQ4. LUCA.
HOGENOMiHOG000203818.
InParanoidiQ9S9T7.
KOiK12184.
OMAiGNFITVM.
OrthoDBiEOG09360Q4L.
PhylomeDBiQ9S9T7.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S9T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMEVKLWNDK REREMYENFA ELFAIIKATE KLEKAYIRDL INPSEYESEC
60 70 80 90 100
QKLIVHFKTL SATLKDTVPN IERFADTYKM DCPAALYRLV TSGLPATVEH
110 120 130 140 150
RATVAASTSN SASIVAECVQ NFITSMDSLK LNMVAVDQVY PLLSDLSASL
160 170 180 190 200
NKLSILPPDF EGKTKMKEWL SRLSKMGAAD ELTEQQSRQL HFDLESSYNS
210
FMAALPKAGN
Length:210
Mass (Da):23,689
Last modified:May 29, 2007 - v2
Checksum:i8011A0DAA55DE5FC
GO

Sequence cautioni

The sequence AAD48972 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAB81042 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162444 Genomic DNA. Translation: AAD48972.1. Different initiation.
AL161502 Genomic DNA. Translation: CAB81042.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE82456.1.
CP002687 Genomic DNA. Translation: AEE82457.1.
BT015071 mRNA. Translation: AAT71943.1.
BT015727 mRNA. Translation: AAU45225.1.
AK229295 mRNA. Translation: BAF01158.1.
PIRiH85062.
RefSeqiNP_567281.2. NM_116739.3.
NP_974513.1. NM_202784.2.
UniGeneiAt.33930.
At.72595.

Genome annotation databases

EnsemblPlantsiAT4G05000.1; AT4G05000.1; AT4G05000.
AT4G05000.2; AT4G05000.2; AT4G05000.
GeneIDi825842.
GrameneiAT4G05000.1; AT4G05000.1; AT4G05000.
AT4G05000.2; AT4G05000.2; AT4G05000.
KEGGiath:AT4G05000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162444 Genomic DNA. Translation: AAD48972.1. Different initiation.
AL161502 Genomic DNA. Translation: CAB81042.1. Different initiation.
CP002687 Genomic DNA. Translation: AEE82456.1.
CP002687 Genomic DNA. Translation: AEE82457.1.
BT015071 mRNA. Translation: AAT71943.1.
BT015727 mRNA. Translation: AAU45225.1.
AK229295 mRNA. Translation: BAF01158.1.
PIRiH85062.
RefSeqiNP_567281.2. NM_116739.3.
NP_974513.1. NM_202784.2.
UniGeneiAt.33930.
At.72595.

3D structure databases

ProteinModelPortaliQ9S9T7.
SMRiQ9S9T7. Positions 3-100, 115-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11153. 7 interactions.
IntActiQ9S9T7. 3 interactions.
STRINGi3702.AT4G05000.1.

Proteomic databases

PaxDbiQ9S9T7.
PRIDEiQ9S9T7.

Protocols and materials databases

DNASUi825842.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G05000.1; AT4G05000.1; AT4G05000.
AT4G05000.2; AT4G05000.2; AT4G05000.
GeneIDi825842.
GrameneiAT4G05000.1; AT4G05000.1; AT4G05000.
AT4G05000.2; AT4G05000.2; AT4G05000.
KEGGiath:AT4G05000.

Organism-specific databases

TAIRiAT4G05000.

Phylogenomic databases

eggNOGiKOG3284. Eukaryota.
ENOG4111IQ4. LUCA.
HOGENOMiHOG000203818.
InParanoidiQ9S9T7.
KOiK12184.
OMAiGNFITVM.
OrthoDBiEOG09360Q4L.
PhylomeDBiQ9S9T7.

Miscellaneous databases

PROiQ9S9T7.

Gene expression databases

GenevisibleiQ9S9T7. AT.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVP282_ARATH
AccessioniPrimary (citable) accession number: Q9S9T7
Secondary accession number(s): Q6DBF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.