Q9S9K4 (ASPL2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartic proteinase-like protein 2
EC=3.4.23.-

Gene names

Ordered Locus Names:	At1g65240
ORF Names:	T23K8.15

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Ref.3.
Sequence similarities	Belongs to the peptidase A1 family.
Sequence caution	The sequence AAD26876.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 449	426	Aspartic proteinase-like protein 2		PRO_0000259444
	Propeptide	450 – 475	26	Removed in mature form Potential		PRO_0000259445
Sites
	Active site	92	1	By similarity
	Active site	303	1	By similarity
Amino acid modifications
	Lipidation	449	1	GPI-anchor amidated serine Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9S9K4 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 1DD9C1A5EF147BA0	FASTA	475	51,828
	10 20 30 40 50 60 MELRRKLCIV VAVFVIVIEF ASANFVFKAQ HKFAGKKKNL EHFKSHDTRR HSRMLASIDL 70 80 90 100 110 120 PLGGDSRVDS VGLYFTKIKL GSPPKEYHVQ VDTGSDILWI NCKPCPKCPT KTNLNFRLSL 130 140 150 160 170 180 FDMNASSTSK KVGCDDDFCS FISQSDSCQP ALGCSYHIVY ADESTSDGKF IRDMLTLEQV 190 200 210 220 230 240 TGDLKTGPLG QEVVFGCGSD QSGQLGNGDS AVDGVMGFGQ SNTSVLSQLA ATGDAKRVFS 250 260 270 280 290 300 HCLDNVKGGG IFAVGVVDSP KVKTTPMVPN QMHYNVMLMG MDVDGTSLDL PRSIVRNGGT 310 320 330 340 350 360 IVDSGTTLAY FPKVLYDSLI ETILARQPVK LHIVEETFQC FSFSTNVDEA FPPVSFEFED 370 380 390 400 410 420 SVKLTVYPHD YLFTLEEELY CFGWQAGGLT TDERSEVILL GDLVLSNKLV VYDLDNEVIG 430 440 450 460 470 WADHNCSSSI KIKDGSGGVY SVGADNLSSA PRLLMITKLL TILSPLIVMA FTSLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed: 16602701] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007230 Genomic DNA. Translation: AAD26876.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE34347.1.
IPI	IPI00544994.
PIR	E96676.
RefSeq	NP_176703.1. NM_105197.2.
UniGene	At.11165. At.35907.
3D structure databases
HSSP	HSSP built from PDB template 1FMX based on UniProtKB P07267.
ProteinModelPortal	Q9S9K4.
SMR	Q9S9K4. Positions 69-424.
ModBase	Search...
Protein family/group databases
MEROPS	A01.A37.
Proteomic databases
PRIDE	Q9S9K4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G65240.1; AT1G65240.1; AT1G65240.
GeneID	842831.
GenomeReviews	Gene locus AT1G65240 in contig CT485782_GR.
KEGG	ath:AT1G65240.
NMPDR	fig|3702.1.peg.5945.
Organism-specific databases
TAIR	At1g65240.
Phylogenomic databases
eggNOG	KOG1339.
GeneTree	EPGT00070000028262.
HOGENOM	HBG317822.
InParanoid	Q9S9K4.
OMA	YADESTS.
PhylomeDB	Q9S9K4.
ProtClustDB	CLSN2914670.
Gene expression databases
Genevestigator	Q9S9K4.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPL2_ARATH

Accession

Primary (citable) accession number: Q9S9K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	December 14, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program