Reviewed, UniProtKB/Swiss-Prot Q9S8P4 (RHRE_PEA)

Names and origin

Protein names

Recommended name:
    Rhicadhesin receptor
Alternative name(s):
    Germin-like protein

Gene names

Name:

GER1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative receptor for bacterial rhicadhesin, an attachment protein of rhizobiaceae.
Subcellular location	Secreted › extracellular space › apoplast. Secreted › cell wall.
Post-translational modification	Glycosylated.
Sequence similarities	Belongs to the germin family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.2
	Chain	21 – 217	197	Rhicadhesin receptor		PRO_0000010842
Sites
	Metal binding	107	1	Manganese By similarity
	Metal binding	109	1	Manganese By similarity
	Metal binding	114	1	Manganese By similarity
	Metal binding	153	1	Manganese By similarity
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 45		By similarity
Experimental info
	Sequence conflict	45	1	C → S AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9S8P4-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: FC0D0C7BA22ECCC7	FASTA	217	22,978
	10 20 30 40 50 60 MKLIAVLLLV VLATATTATA ADADALQDLC VADYASVILV NGFACKPASN VTAEDFFSNL 70 80 90 100 110 120 LVKQGATNNT FGSLVTGANV QRIPGLNTLG VSMARIDYAP GGLNPPHTHP RATEMVFVLE 130 140 150 160 170 180 GQLDVGFITT TNQLIAKTIA KGETFVFPKG LVHFQKNNGW EPATVIAGFN SQLPGTVNIP 190 200 210 LTLFNATPPV PDNVLTKAFQ IGTKEVQKIK SKFAPKK

References

[1]	"Identification of an oxalate oxidase activity in pea roots, and cloning of cDNAs encoding germin-like proteins." Wisniewski J.P., Bornemann S., Brewin N.J. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wisconsin Perfection. Tissue: Root.
[2]	"Purification and partial characterization of a glycoprotein from pea (Pisum sativum) with receptor activity for rhicadhesin, an attachment protein of Rhizobiaceae." Swart S., Logman T.J., Smit G., Lugtenberg B.J., Kijne J.W. Plant Mol. Biol. 24:171-183(1994) [PubMed: 8111015] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-45.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ250832 mRNA. Translation: CAB65369.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.15.1.1. 287.
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
SMART	SM00835. Cupin_1. 1 hit. [Graphical view]
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHRE_PEA

Accession

Primary (citable) accession number: Q9S8P4
Secondary accession number(s): Q9SC40

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	November 24, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)