Reviewed, UniProtKB/Swiss-Prot Q9S841 (PSBO2_ARATH)

Names and origin

Protein names

Recommended name:
    Oxygen-evolving enhancer protein 1-2, chloroplastic
      Short name=OEE1
Alternative name(s):
    33 kDa subunit of oxygen evolving system of photosystem II
    OEC 33 kDa subunit
    33 kDa thylakoid membrane protein
    Manganese-stabilizing protein 2
      Short name=MSP-2

Gene names

Name:	PSBO2
Ordered Locus Names:	At3g50820
ORF Names:	F18B3.100

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stabilizes the manganese cluster which is the primary site of water splitting. Regulates dephosphorylation and turnover of the PSII reaction center D1 protein. Ref.6 Ref.7
Subcellular location	Plastid › chloroplast thylakoid membrane. Note: Associated with the photosystem II complex.
Sequence similarities	Belongs to the psbO family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Domain	Transit peptide
Ligand	Manganese
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	photoinhibition Ref.7 Inferred from mutant phenotype. Source: TAIR photosystem II assembly Ref.6 Inferred from mutant phenotype. Source: TAIR photosystem II stabilization Ref.6 Inferred from mutant phenotype. Source: TAIR regulation of protein amino acid dephosphorylation Ref.7 Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR extrinsic to membrane Inferred from electronic annotation. Source: InterPro oxygen evolving complex Inferred from electronic annotation. Source: InterPro plastoglobule Inferred from direct assay. Source: TAIR thylakoid lumen Inferred from direct assay. Source: TAIR
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW poly(U) binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449424,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 57	57	Chloroplast By similarity
	Transit peptide	58 – 84	27	Thylakoid
	Chain	85 – 331	247	Oxygen-evolving enhancer protein 1-2, chloroplastic		PRO_0000029554

Sequences

	Sequence		Length	Mass (Da)
	Q9S841-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 08F837E651B1C2E7	FASTA	331	35,019
	10 20 30 40 50 60 MATSLQAAAT FLQPAKIAAS PSRNVHLRSN QTVGKSFGLD SSQARLTCSL HSDLKDFAGK 70 80 90 100 110 120 CSDAAKIAGF ALATSALVVS GAGAEGAPKR LTYDEIQSKT YMEVKGTGTA NQCPTIDGGS 130 140 150 160 170 180 ETFSFKAGKY TGKKFCFEPT SFTVKADSVS KNAPPDFQNT KLMTRLTYTL DEIEGPFEVG 190 200 210 220 230 240 SDGSVKFKEE DGIDYAAVTV QLPGGERVPF LFTVKQLEAS GKPESFSGKF LVPSYRGSSF 250 260 270 280 290 300 LDPKGRGGST GYDNAVALPA GGRGDEEELS KENVKNTAAS VGEITLKITK SKPETGEVIG 310 320 330 VFESLQPSDT DLGAKVPKDV KIQGVWYGQI E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences and map position of 31 Arabidopsis thaliana cDNAs encoding organellar polypeptides." Legen J., Misera S., Herrmann R.G., Altschmied L. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"The manganese-stabilizing protein is required for photosystem II assembly/stability and photoautotrophy in higher plants." Yi X., McChargue M., Laborde S., Frankel L.K., Bricker T.M. J. Biol. Chem. 280:16170-16174(2005) [PubMed: 15722336] [Abstract] Cited for: FUNCTION.
[7]	"The Arabidopsis PsbO2 protein regulates dephosphorylation and turnover of the photosystem II reaction centre D1 protein." Lundin B., Hansson M., Schoefs B., Vener A.V., Spetea C. Plant J. 49:528-539(2007) [PubMed: 17217465] [Abstract] Cited for: FUNCTION.
[8]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ145957 mRNA. Translation: CAB53092.1. AL049862 Genomic DNA. Translation: CAB42911.1. AY050362 mRNA. Translation: AAK91379.1. AY116934 mRNA. Translation: AAM51568.1. AY088799 mRNA. Translation: AAM67110.1.
IPI	IPI00519769.
PIR	T08403.
RefSeq	NP_190651.1.
UniGene	At.21117
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9S841. 1 interaction.
2-D gel databases
SWISS-2DPAGE	Q9S841.
Proteomic databases
PRIDE	Q9S841.
ProMEX	Q9S841.
Genome annotation databases
GeneID	824246.
GenomeReviews	Gene locus AT3G50820 in contig BA000014_GR.
KEGG	ath:AT3G50820.
NMPDR	fig|3702.1.peg.16332.
Organism-specific databases
TAIR	At3g50820.
Phylogenomic databases
OMA	Q9S841. GKANDCP.
Gene expression databases
ArrayExpress	Q9S841.
GermOnline	AT3G50820. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002628. PSII_MSP. [Graphical view]
Pfam	PF01716. MSP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSBO2_ARATH

Accession

Primary (citable) accession number: Q9S841

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)